Search Results (91)

Tubastraea coccinea and T. tagusensis, commonly known as sun corals, are two species of stony corals (Scleractinia, Dendrophylliidae) native to the Indo-Pacific region (T. coccinea) and the Galapagos Islands (T. tagusensis), respectively. They are considered highly invasive species, particularly in the Western Atlantic Ocean, due to high adaptability to various ecological conditions and notable resilience. Given their demonstrated invasiveness, it is important to delve into their physiology and the molecular bases supporting their resilience. However, to date, only a few molecular tools are available for the study of these organisms. The primary objective of the present study was the development of an efficient RNA extraction protocol for Tubastraea coccinea and T.a tagusensis samples collected off Ilha Grande Bay, Rio de Janeiro (Brazil). The quantity of isolated RNA was evaluated using NanoDrop, while its purity and quality were determined by evaluating the A260/A280 and A260/230 ratios. Subsequently, based on genes known for T. coccinea, two housekeeping genes and seven stress response-related genes were isolated and characterized, for the first time for both species, using a molecular approach. An interactomic analysis was also conducted, which revealed functional interactions among these genes. This study represents the first report on gene networks in Tubastraea spp., opening new perspectives for understanding the chemical ecology and the cellular mechanisms underlying the invasiveness of these species. The results obtained will be useful for ecological conservation purposes, contributing to the formulation of strategies to limit their further expansion. Full article

The combustion of ammonia with oxygen presents a promising pathway for global energy transformation using carbon dioxide-neutral energy solutions and carbon capture. Ammonia, a carbon-free fuel, offers several benefits, owing to its non-explosive nature, high octane rating, and ease of storage and distribution. However, challenges such as low flammability and excessive nitrogen oxide (NO_x) emissions must be addressed. This paper explores the recent advances in ammonia oxy-combustion and highlights recent experimental and numerical research on NO_x emission traits, combustion, and flame propagation across various applications, including gas furnaces, internal combustion engines, and boilers. Furthermore, this review discusses the diverse approaches to overcoming the challenges of ammonia combustion, including oxygen enrichment, fuel blending, plasma assistance, preheating, multiple injections, and burner design modifications. By summarizing the advancements in ammonia oxy-combustion investigation, this paper aims to provide valuable insights that can serve as reference information for prospective ammonia oxy-combustion research and applications toward the transition to sustainable energy. Full article

A revision of the submarine instability processes offshore the Campania region is presented herein based on the literature data and Multibeam bathymetric and seismic profiles previously acquired by the CNR ISMAR of Naples (Italy). Among others, the objectives and perspectives of this research include the following: the chrono-stratigraphic framework of the submarine instability events and their correlation with the trigger geological processes, including the seismicity, the volcanism and the tectonic activity; density reversal has not been detected as a control factor; the implementation of technologies and database for the acquisition and the processing of morpho-bathymetric, seismo-stratigraphic and sedimentological data in the submarine slopes of Campania, characterized by submarine gravitational instabilities. Other main tasks include producing thematic geomorphological maps of the submarine slopes associated with instability phenomena. The principles of slope stability have been revised to be independent of the slope height. In submarine slopes mainly composed of sand, the stability depends on the slope inclination angle concerning the horizontal (β), equal or minor to the internal friction angle of loose sand (ϕ). Based on this research, it can be outlined that the submarine instability processes offshore of Campania mainly occur along the flanks of volcanic edifices, both emerged (Ischia) and submerged (Pentapalummo, Nisida, Miseno, Procida Channel), on steep, tectonically-controlled sedimentary slopes, (southern slope of Sorrento Peninsula, slope of the Policastro Gulf), and on ramps with a low gradient that surround wide continental shelves (Gulf of Salerno). Full article

Background/Objectives: Prostatic lymphoma is a rare malignant tumour that frequently causes urinary tract obstruction. It is uncommon for patients to present with systemic features or B-symptoms. As a result, it is often diagnosed incidentally during surgical lower urinary tract symptoms (LUTS) treatment. This systematic review aims to identify any common clinical features of prostatic lymphoma diagnosed incidentally during surgical LUTS treatment and summarise disease treatment and outcomes. Methods: The study protocol was registered with Prospective Register of Systematic Reviews (PROSPERO). A search was performed across the following electronic databases: MEDLINE, Embase, Web of Science, and Cochrane Database of Systematic Reviews. Full texts of eligible studies were analysed and data were extracted. The review was performed in accordance with PRISMA guidelines. Results: A total of 24 case reports compromising 25 cases were included. The median (IQR) age was 67 (61–73) years. All patients reported LUTS as their primary complaint, and the median duration of LUTS prior to diagnosis was 17 (4–44) months. Serum prostate-specific antigen (PSA) was normal in 10 cases and prostatomegaly present on imaging in 16 cases. A total of 10 different subtypes of lymphoma were reported. Extra-prostatic involvement was reported in eight patients. Chemotherapy, with or without adjuvant radiotherapy, was the mainstay of lymphoma treatment. The majority of articles reported positive outcomes, with complete remission in 17 cases. Conclusions: Prostatic lymphoma is a difficult clinical diagnosis due to its similar presentation to benign prostatic hyperplasia (BPH). Although rare, prostatic lymphoma may need to be considered as a diagnosis in patients with an atypical presentation of BPH. Prognosis is often favourable after prompt referral to haematology or oncology. Full article

Plastics play a key role in every sector of the economy, being used in the manufacturing of products in the fields of health, food packaging, and agriculture. Their mismanagement poses a serious threat to ecosystems and, in general, to human life. For this reason, particular attention has been paid in the last decade to the use of biodegradable polymers (BPs) as an alternative to classic plastics. In this study, we aimed to identify bacterial strains able to colonize the surface of five BPs: poly(butylene succinate) (PBS), poly(butylene succinate-co-butylene adipate) (PBSA), poly(ε-caprolactone), (PCL), poly(3-hydroxybutyrate) (PHB), and poly(lactic acid) (PLA). For this experiment, mesocosms were designed ad hoc to mimic the conditions in which the polymers can be found in marine environments: i. suspended in the water column; ii. laying over gravel; and iii. under gravel. Four bacterial samples were taken (3, 4, 10, and 12 months from the start of the experiment) from five BPs incubated in the above-mentioned three conditions. Our results demonstrated that bacteria belonging to the Proteobacteria, Actinobacteria, Firmicutes, Bacillota, Bacteroidota, and Cyanobacteria phyla were the most frequent colonizers of the surfaces of the five polymers under analysis, and could be responsible for their degradation, resulting in the evolution of strategies to degrade plastics through the secretion of specific enzymes. Full article

Introduction: Future evolutions of artificial intelligence (AI) will support autonomous surgery, conducted without the need for human decision making and implementation, but we have not yet achieved this level of technology. Presently, the predominant applications of AI in urological surgery are achieved using the tool of computer vision. This review aims to summarise potential intra-operative AI tools for urologists. Method: A systematic search was conducted through Scopus, PubMed, Embase, and Medline by two independent reviewers, with a third to resolve any conflicts. As a rule, only original articles describing the use or potential use of artificial intelligence intra-operatively in urologic surgery were included. A total of 60 articles were reviewed. Key content and findings: There is significant research investigating the ability to diagnose bladder tumours using AI assistance at the time of cystoscopy, with studies showing the ability to also grade tumour based on appearance and differentiate between carcinoma in situ and indeterminate lesions. With the aid of AI, kidney stones can accurately be identified and diagnosed morphologically intra-operatively. Various studies show the ability to overlay 2D and 3D anatomical models on a surgeon’s screen, as well as correctly identify important anatomical landmarks and surgical instruments, with AI support. All types of intra-operative data can be analysed with AI to assess surgeon performance, predict post-operative outcomes such as continence post prostatectomy, and recognise complications such as bleeding and ischemia. Conclusions: AI holds great potential for urologists during surgery to improve safety, diagnostic accuracy, identification of anatomical structures and surgical instruments, assessment of the surgeon for self-evaluation, and prediction of post-operative outcomes. Before the use of AI as an aid during surgery becomes standard practice, more prospective studies are needed to evaluate its real-world application, feasibility, and costs. Full article

The common octopus (Octopus vulgaris), among coleoid cephalopods, has evolved the most complex nervous system and sophisticated behaviors. Historically, O. vulgaris was a key animal model for neurophysiology research, and today, it is studied for its genomic innovations. However, unlike other models, there is no octopus farming for research, so specimens must be collected from the wild. This study assessed the impact of fishing on octopuses used in research, considering those caught using artisanal pots in the ‘Regno di Nettuno’ Marine Protected Area, Ischia (NA). To evaluate fishing stress, we identified morphological stress indicators such as chromatophore pattern and posture, and three potential molecular markers, estrogen receptor (ER), catalase (CAT), and heat shock protein (HSP70). We measured the percentage of stress signals shown by fished specimens and analyzed their differential gene expression. The transcriptional levels of octopuses caught using traps were compared to control specimens acclimated in captivity. Results indicated fluctuations in gene expression due to fishing stress. These findings suggest that an acclimation period after the stress event of fishing is crucial for ensuring the welfare of octopuses used in research, thus enhancing the quality of physiological and ethological studies. Full article

The growing need to address natural and human-induced disasters while protecting territory remains a key focus for the scientific community. Effective emergency management, especially during wildfires, requires coordinated responses to safeguard lives and assets. This study develops hazard maps to aid emergency planning in Italy and estimate territorial resilience indicators. Focusing on wildfire ignition hazards in Ischia, the study uses a probabilistic model based on fifteen years of wildfire data (2009–2023). By analyzing ignition points and employing a Poisson distribution, it correlates ignition probabilities with vegetation types. The hazard maps reveal that wildfire risk is primarily influenced by the wildland–urban interface and vegetation characteristics, emphasizing the need to integrate territorial and urban factors into wildfire forecasting. The findings also suggest areas for refining the model to enhance risk mitigation strategies. Full article

Background: stereotactic ablative body radiotherapy (SABR) is a disruptive radiation therapy technique which is increasingly used for the treatment of urologic cancers. The aim of this narrative review is to provide an overview on the current landscape of SABR in urologic cancers and highlight advancements on the horizon. Methods: a narrative review of the contemporary role of SABR in urologic cancers is conducted. Results: in localised prostate cancer, SABR boasts excellent tumour control and biochemical control, with acceptable GU and GI toxicity. Its comparison to laparoscopic radical prostatectomy is currently ongoing. SABR appears to be practical for metastasis-directed therapy in metastatic prostate cancer, with good local control and a low toxicity profile, either alone or in combination with ADT. In localised RCC, SABR offers adequate local control with a modest impact on renal function in patients unfit for surgical management. Its role in metastatic RCC is much more established, where it has been shown to be superior to conventional radiotherapy. Emerging evidence suggests that SABR has a role in delaying systemic therapy whilst maintaining QOL and overall survival. Intriguingly, in metastatic prostate cancer and metastatic RCC, SABR results in a cytoreductive and immunomodulatory ‘abscopal effect’, a focus of current investigations. Conclusions: SABR has emerged as a safe, effective, and feasible treatment for urologic cancers. Urologists should be aware of its increasing use in localised prostate cancer and metastatic RCC, with good oncological outcomes combined with acceptable toxicity. In addition, SABR holds promise for both metastatic prostate cancer and localised RCC treatment in terms of toxicity and oncological outcomes. Full article

This paper aims to provide a framework for guiding organizations toward Agile practices for project management. Through a case study analysis conducted in an information technology company, the paper aims to explore the interplay between current organization structure and project management approaches, the set of competencies, and the cultural change required for a transition toward Agile transformation. The paper provides a framework comprising four main blocks necessary for guiding organizations toward Agile practices for successful project management. The framework proposed demonstrates that for organizations to be nimble and to improve teamwork performance, it is necessary to undertake a set of changes in culture, competencies, structure, and approaches. The findings highlight the importance of a structured approach to Agile transformation, with particular emphasis on top management support and continuous learning. By formalizing Agile initiatives through structured approaches and continuous engagement, organizations can foster a shared sense of purpose among team members, ensuring sustained commitment and facilitating smoother transitions. Full article

We conducted large-depth Ground-Penetrating Radar investigations of the seismogenic Casamicciola fault system at the volcanic island of Ischia, with the aim of constraining the source characteristics of this active and capable fault system. On 21 August 2017, a shallow (hypocentral depth of 1.2 km), moderate (M_d = 4.0) earthquake hit the island, causing severe damage and two fatalities. This was the first damaging earthquake recorded on the volcanic island of Ischia from the beginning of the instrumental era. Our survey was performed using the Loza low-frequency (15–25 MHz) GPR system calibrated by TDEM results. The data highlighted variations in the electromagnetic signal due to the presence of contacts, i.e., faults down to a depth larger than 100 m below the surface. These signal variations match with the position of the synthetic and antithetic active fault system bordering the Casamicciola Holocene graben. Our study highlights the importance of employing large-depth Ground-Penetrating Radar geophysical techniques for investigating active fault systems not only in their shallower parts, but also down to a few hundred meters’ depth, providing a contribution to the knowledge of seismic hazard studies on the island of Ischia and elsewhere. Full article

Epiphytic diatoms growing in Mediterranean seagrass meadows, particularly those of the genus Cocconeis, are abundant and ecologically significant, even in naturally acidified environments. One intriguing aspect of some benthic diatoms is their production of an unidentified cell-death-promoting compound, which induces destruction of the androgenic gland in Hippolyte inermis Leach, 1816, a shrimp exhibiting protandric hermaphroditism, principally under normal environmental pH levels. The consumption of Cocconeis spp. by this shrimp is vital for maintaining the stability of its natural populations. Although many attempts have been made to reveal the identity of the apoptotic compound, it is still unknown. In this study, we strategically integrated a bioactivity-based fractionation, a metabolomic approach, and two different experimental avenues to identify potential apoptotic metabolites from Cocconeis scutellum var. parva responsible for the sex reversal in H. inermis. Our integrated analysis uncovered two potential candidate metabolites, one putatively identified as a lysophosphatidylglycerol (LPG) (16:1) and the other classified as a fatty acid ester. This is the first time LPG (16:1) has been reported in C. scutellum var. parva and associated with cell-death processes. These candidate metabolites mark substantial progress in elucidating the factors responsible for triggering the removal of the androgenic gland in the early post-larval phases of H. inermis. Full article

Marine sponges represent a good source of natural metabolites for biotechnological applications in the pharmacological, cosmeceutical, and nutraceutical fields. In the present work, we analyzed the biotechnological potential of the alien species Haliclona (Halichoclona) vansoesti de Weerdt, de Kluijver & Gomez, 1999, previously collected in the Mediterranean Sea (Faro Lake, Sicily). The bioactivity and chemical content of this species has never been investigated, and information in the literature on its Caribbean counterpart is scarce. We show that an enriched extract of H. vansoesti induced cell death in human melanoma cells with an IC₅₀ value of 36.36 µg mL⁻¹, by (i) triggering a pro-inflammatory response, (ii) activating extrinsic apoptosis mediated by tumor necrosis factor receptors triggering the mitochondrial apoptosis via the involvement of Bcl-2 proteins and caspase 9, and (iii) inducing a significant reduction in several proteins promoting human angiogenesis. Through orthogonal SPE fractionations, we identified two active sphingoid-based lipid classes, also characterized by nuclear magnetic resonance and mass spectrometry, as the main components of two active fractions. Overall, our findings provide the first evaluation of the anti-cancer potential of polar lipids isolated from the marine sponge H. (Halichoclona) vansoesti, which may lead to new lead compounds with biotechnological applications in the pharmaceutical field. Full article

The protandric shrimp Hippolyte inermis is the only known marine invertebrate whose sex determination is strongly influenced by the composition of its food. In H. inermis, a sex reversal is triggered by the ingestion of diatoms of the genus Cocconeis associated with leaves of the seagrass Posidonia oceanica. These diatoms contain compounds that promote programmed cell death (PCD) in H. inermis and also in human cancer cells. Transcriptomic analyses suggested that ferroptosis is the primary trigger of the shrimp’s sex reversal, leading to the rapid destruction of the androgen gland (AG) followed by a chain of apoptotic events transforming the testes into ovaries. Here, we propose a molecular approach to detect the effects of compounds stimulating the PCD. An RNA extraction method, suitable for young shrimp post-larvae (five days after metamorphosis; PL₅ stage), was established. In addition, six genes involved in apoptosis, four involved in ferroptosis, and seven involved in the AG switch were mined from the transcriptome, and their expression levels were followed using real-time qPCR in PL₅ fed on Cocconeis spp., compared to PL₅ fed on a basic control feed. Our molecular approach, which detected early signals of sex reversal, represents a powerful instrument for investigating physiological progression and patterns of PCD in marine invertebrates. It exemplifies the physiological changes that may start a few days after the settlement of post-larvae and determine the life destiny of an individual. Full article