Search Results (12)

Prescriptions for fuel management are universally applied across the forest types in British Columbia, Canada, to reduce the fire behaviour potential in the wildland–urban interface. Fuel thinning treatments have been advocated as a means of minimizing the likelihood of crown fire development in conifer forests. We hypothesized that these types of prescriptions are inappropriate for the coastal rainforests of the Whistler region of the province. Our study examined the impact of fuel thinning treatments in four stands located in the Whistler community forest. We measured several in-stand microclimatic variables beginning with snow melt in the spring up to the height of fire danger in late summer, at paired thinned and unthinned stand locations. We found that the thinning led to warmer, drier, and windier fire environments. The difference in mean soil moisture, ambient air temperature, and relative humidity between thinned and unthinned stands was significant in the spring with approximate p-values of 0.000217, 9.40 × 10⁻⁵, and 4.33 × 10⁻⁸, respectively, though there were no discernible differences in the late summer. The difference in mean solar radiation, average wind speed, and average cross wind between thinned and unthinned locations are significant in the spring and late summer (with approximate p-values for spring of 9.54 × 10⁻⁷, 0.02101, 1.92 × 10⁻⁹, and for late summer of 2.45 × 10⁻⁷, 4.08 × 10⁻⁶, and 2.45 × 10⁻⁵, respectively). Full article

Human Lyme borreliosis (LB) represents a multisystem disorder that can progress in stages. The causative agents are transmitted by hard ticks of the Ixodes ricinus complex that have been infected with the spirochete Borrelia burgdorferi sensu lato. Today, LB is considered the most important human tick-borne illness in the Northern Hemisphere. The causative agent was identified and successfully isolated in 1982 and, shortly thereafter, antibiotic treatment was found to be safe and efficacious. Since then, various in vitro studies have been conducted in order to improve our knowledge of the activity of antimicrobial agents against B. burgdorferi s. l. The full spectrum of in vitro antibiotic susceptibility has still not been defined for some of the more recently developed compounds. Moreover, our current understanding of the in vitro interactions between B. burgdorferi s. l. and antimicrobial agents, and their possible mechanisms of resistance remains very limited and is largely based on in vitro susceptibility experiments on only a few isolates of Borrelia. Even less is known about the possible mechanisms of the in vitro persistence of spirochetes exposed to antimicrobial agents in the presence of human and animal cell lines. Only a relatively small number of laboratory studies and cell culture experiments have been conducted. This review summarizes what is and what is not known about the in vitro susceptibility of B. burgdorferi s. l. It aims to shed light on the known unknowns that continue to fuel current debates on possible treatment resistance and mechanisms of persistence of Lyme disease spirochetes in the presence of antimicrobial agents. Full article

The glycoproteins alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) have long been approved as biomarkers for diagnosing and monitoring tumors. International Reference Preparations (IRPs) have been around since 1975. Nevertheless, manufacturer-dependent differences have been reported, indicating a lack of harmonization. This paper analyzes data from 15 external quality assessment (EQA) surveys conducted worldwide between 2018 and 2022. The aim was to gain insight into the longitudinal development of manufacturer-dependent differences for CEA and AFP. In each survey, participating laboratories received two samples with different tumor marker levels. Inter- and intra-assay variability was analyzed and the mean 80% and 90% of the manufacturer collectives were compared to the evaluation criteria of the German Medical Association (RiliBÄK). The median EQA results for CEA revealed manufacturer-dependent differences between the highest and lowest collective of up to 100%; for AFP, the median differences mostly remained below 40%. The coefficients of variation were predominantly low for both markers. We concluded that the current assays for AFP and CEA detection are better harmonized than previously reported. The assays displayed a good robustness; however, a narrowing of the current assessment limits in EQA schemes could further enhance the quality of laboratory testing. Full article

Subalpine fir mortality and stand decline are increasingly evident in British Columbia (B.C.). This long-term study confirmed Dryocoetes confusus to be the major disturbance agent in high-elevation subalpine forests, killing over two-thirds of subalpine fir in eleven one-hectare study plots. D. confusus infestations in mature stands can be described as early-, mid-, or late-phase. The transition from the early- through late-phase is characterized by a lowered stem density as high levels of D. confusus attack and remove the largest trees, while other mortality factors kill smaller trees. Initially, live subalpine fir density and D. confusus activity varied among plots. By a final assessment, very little difference was observed in live stems per hectare. Mortality from all factors ranged from 0.5% to 5% annually, reaching as high as 80% in-stand mortality with >6 times more dead than live volume. When subalpine fir density was reduced to <400 sph, the D. confusus attack rate declined. Cumulative mortality increased the average gap size in plots from 11 m² to 18 m². Our study also showed that D. confusus might be able to switch to a univoltine life cycle, taking advantage of warmer and longer growing seasons that, in part, could explain the rapid increase in mortality in stands. Full article

Babesiosis is attracting increasing attention as a worldwide emerging zoonosis. The first case of human babesiosis in Europe was described in the late 1950s and since then more than 60 cases have been reported in Europe. While the disease is relatively rare in Europe, it is significant because the majority of cases present as life-threatening fulminant infections, mainly in immunocompromised patients. Although appearing clinically similar to human babesiosis elsewhere, particularly in the USA, most European forms of the disease are distinct entities, especially concerning epidemiology, human susceptibility to infection and clinical management. This paper describes the history of the disease and reviews all published cases that have occurred in Europe with regard to the identity and genetic characteristics of the etiological agents, pathogenesis, aspects of epidemiology including the eco-epidemiology of the vectors, the clinical courses of infection, diagnostic tools and clinical management and treatment. Full article

Due to globally rising numbers of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections, resources for real-time reverse-transcription polymerase chain reaction (rRT-PCR)-based testing have been exhausted. In order to meet the demands of testing and reduce transmission, SARS-CoV-2 antigen-detecting rapid diagnostic tests (Ag-RDTs) are being considered. These tests are fast, inexpensive, and simple to use, but whether they detect potentially infectious cases has not been well studied. We evaluated three lateral flow assays (RIDA^®QUICK SARS-CoV-2 Antigen (R-Biopharm), SARS-CoV-2 Rapid Antigen Test (Roche)), and NADAL^® COVID-19 Ag Test (Nal von Minden GmbH, Regensburg, Germany) and one microfluidic immunofluorescence assay (SARS-CoV-2 Ag Test (LumiraDx GmbH, Cologne, Germany)) using 100 clinical samples. Diagnostic rRT-PCR and cell culture testing as a marker for infectivity were performed in parallel. The overall Ag-RDT sensitivity for rRT-PCR-positive samples ranged from 24.3% to 50%. However, for samples with a viral load of more than 6 log₁₀ RNA copies/mL (22/100), typically seen in infectious individuals, Ag-RDT positivity was between 81.8% and 100%. Only 51.6% (33/64) of the rRT-PCR-positive samples were infectious in cell culture. In contrast, three Ag-RDTs demonstrated a more significant correlation with cell culture infectivity (61.8–82.4%). Our findings suggest that large-scale SARS-CoV-2 Ag-RDT-based testing can be considered for detecting potentially infective individuals and reducing the virus spread. Full article

An experimental fire was conducted in one-year-old mulched (masticated) boreal fuels, where all aboveground biomass was mulched with no stems removed or left standing. Typical mulching practices remove remnant biomass; leaving biomass in situ reduces overall management input. While fuel quantities were not explicitly reduced, availability of fuels to fire was reduced. Infrared imagery was obtained to quantify rate of spread and intensity to a 1 m resolution. In-stand totalizing heat flux sensors allowed for the observation of energy release near the surface. When compared with the pre-treatment fuel-type M-2 (mixedwood, 50% conifer), rates of spread were reduced 87% from an expected 8 m min⁻¹ to observed values 1.2 m min⁻¹. Intensity was also reduced from 5000 kWm⁻¹ to 650kWm⁻¹ on average. Intermittent gusts caused surges of fire intensity upwards of 5000 kW m⁻¹ as captured by the infrared imagery. With reference to a logging slash fuel type, observed spread rates declined by 87% and intensity 98%. Independent observations of energy release rates from the radiometers showed similar declines. As mulching is a prevalent fuel management technique in Alberta, Canada, future studies will contribute to the development of a fire behaviour prediction model. Full article

A 3.6 ha experimental fire was conducted in a black spruce peatland forest that had undergone thinning the year prior. After 50 m of spread in a natural stand at 35–60 m min⁻¹, the crown fire (43,000 kW m⁻¹ intensity using Byram’s method) encountered the 50% stem removal treatment; spread rates in the treatment were 50–60 m min⁻¹. Fuel consumption in the control (2.75 kg m⁻²) was comparable to the treatment (2.35 kg m⁻²). Proxy measurements of fire intensity using in-stand heat flux sensors as well as photogrammetric flame heights had detected intensity reductions to 30–40% of the control. Crown fuel load reductions (compensated by higher surface fuel load) appear to be the most significant contributor to the decline in intensity, despite drier surface fuels in the treatment. The burn depth of 5 cm in moss and organic soil did not differ between control and treatment. These observations point to the limited effectiveness (likely reductions in crown fire intensity but not spread rate) of stem removal in boreal black spruce fuel types with high stem density, low crown base height and high surface fuel load. The observed fire behaviour impacts differ from drier conifer forests across North America. Full article

Mechanized cut-to-length forest operations often rely on the use of brush mats created from harvesting debris (tree limbs, tops, and foliage) to reduce soil disturbances as a result of in-stand machine traffic. These brush mats, placed directly on the forest floor within machine operating trails, distribute loads of timber harvesting and extraction machinery to a greater area, thereby reducing peak pressures exerted to the ground and rutting for maintaining technical trafficability of operating trails. Forest biomass has also been promoted as a source of green and renewable energy, to reduce carbon emissions from energy production. However, to maintain sufficient quality of biomass for bioenergy operations (high heating value and low ash content), brush needs to be free of contaminants such as mineral soil. This constraint eliminates the possibility of the dual use of brush, first as a soil protective layer on machine operating trails and afterwards for bioenergy generation. Leaving machine operating trails uncovered will cause machine loads to be fully and directly applied to the soil, thus increasing the likelihood of severe soil disturbance, tree growth impediment and reducing trail trafficability. The main objective of this study was to quantify the effect of varying machine operating trail spacing and width on the amount of brush required for soil protection. This was achieved by creating five model forest stands (four mature and one immature), commonly found in New Brunswick, Canada, and using their characteristics as input in the Biomass Opportunity and Supply Model (BiOS) from FPInnovations. BiOS provided several key biomass related outputs allowing the determination of the amount of biomass available for soil protection, which was the main focus of this research. The simulation results showed that regardless of trail area tested, all four mature forest stands were able to support uniform distribution of 20 kg m⁻² brush mats (green mass) throughout their entire trail network during clear-cut operations but not during partial harvests. From the three factors assessed (brush amount, trail width, and trail spacing), trail width had the highest effect on the required brush amount for trail protection, which in turn has a direct impact on the amount of brush that could be used for bioenergy generation. Full article

New techniques have recently appeared that can extend the advantages of grapple yarding to fast-growing plantations. The most promising technique consists of an excavator-base un-guyed yarder equipped with new radio-controlled grapple carriages, fed by another excavator stationed on the cut-over. This system is very productive, avoids in-stand traffic, and removes operators from positions of high risk. This paper presents the results of a long-term study conducted on 12 different teams equipped with the new technology, operating in the fast-growing black wattle (Acacia mangium Willd) plantations of Sarawak, Malaysia. Data were collected continuously for almost 8 months and represented 555 shifts, or over 55,000 cycles—each recorded individually. Production, utilization, and machine availability were estimated, respectively at: 63 m³ per productive machine hour (excluding all delays), 63% and 93%. Regression analysis of experimental data yielded a strong productivity forecast model that was highly significant, accounted for 50% of the total variability in the dataset and was validated with a non-significant error estimated at less than 1%. The figures reported in this study are especially robust, because they were obtained from a long-term study that covered multiple teams and accumulated an exceptionally large number of observations. Full article

Using biological fertilizers and pesticides based on beneficial soil microbes in order to reduce mineral fertilizers and chemical pesticides in conventional agriculture is still a matter of debate. In this regard, a European research project seeks to elucidate the role of root-endophytic fungi and to develop molecular tools to trace and quantify these fungi in the rhizosphere and root tissue. To do this, the draft genome sequence of the biocontrol fungus Trichoderma virens (T. virens) was screened for simple sequence repeats (SSRs) and primers were developed for 12 distinct loci. Primers were evaluated using a global collection of ten isolates where an average of 7.42 alleles per locus was detected. Nei’s standard genetic distance ranged from 0.18 to 0.27 among the isolates, and the grand mean of haploid diversity in AMOVA analysis was 0.693 ± 0.019. Roots of tomato plants were inoculated with different strains and harvested six weeks later. Subsequent PCR amplification identified root-endophytic strains and co-colonization of roots by different strains. Markers were applied to qPCR to quantify T. virens strains in root tissue and to determine their identity using allele-specific melting curve analysis. Thus, the root-endophytic lifestyle of T. virens was confirmed, strains in roots were quantified and simultaneous colonization of roots by different strains was observed. Full article

Fire severity varies with forest composition and structure, reflecting micrometeorology and the fuel complex, but their respective influences are difficult to untangle from observation alone. We quantify the differences in fire weather between different forest types and the resulting differences in modeled fire behavior. Collection of in-stand weather data proceeded during two summer periods in three adjacent stands in northern Portugal, respectively Pinus pinaster (PP), Betula alba (BA), and Chamaecyparis lawsoniana (CL). Air temperature, relative humidity and wind speed varied respectively as CL < PP < BA, PP < CL < BA, and CL < BA < PP. Differences between PP and the other types were greatest during the warmest and driest hours of the day in a sequence of 10 days with high fire danger. Estimates of daytime moisture content of fine dead fuels and fire behavior characteristics for this period, respectively, from Behave and BehavePlus, indicate a CL < BA < PP gradient in fire potential. High stand density in CL and BA ensured lower wind speed and higher fuel moisture content than in PP, limiting the likelihood of an extreme fire environment. However, regression tree analysis revealed that the fire behavior distinction between the three forest types was primarily a function of the surface fuel complex, and more so during extreme fire weather conditions. Full article