Search Results (17)

The agrarian continent of Africa has many fruits with nutritional, medicinal and spiritual values. Regardless, Africa leads the statistics of poor healthcare globally. Two major challenges in Africa’s healthcare system are poor access and the high cost of medical healthcare. Among others, the effects of such challenges include low responsiveness to medical treatment and a high mortality rate. However, it seems the nosophobia that accompanied the global mortality rate during the COVID-19 pandemic may have triggered a spiritually influenced alternative. One of the traditional alternatives was a subscription to Garcinia Kola, popularly known as Bitter Kola (BK). This article, majoring in spiritual and not psychological influence, raised a hypothetical question: does spirituality influence Africans’ traditional response to COVID-19? To answer this question, Sunnyside in Pretoria was chosen as a demography to investigate the hypothesis. Data were collected via mixed research methods. There were 16 qualitative respondents, including sellers, herbalists and clergies, and 75 consumers as quantitative respondents under probability sampling. The results analysed using Excel and Python’s regression analysis demonstrated strong connections between consumers’ spiritual motivations, the sales period, the sales rate, and the swift traditional response to the pandemic and related illnesses. The outcome validated the influence of spirituality on 60.9% of quantitative respondents and showed how 25–72% responded to COVID-19 symptoms with BK. Likewise, 87.5% of qualitative respondents consumed BK via indigenous spiritual knowledge in response to the pandemic. Subsequently, this article discussed the benefits, limitations and lessons of spiritual influence on BK consumption in the post-COVID-19 era. Full article

There are various herbicides which were used in the agriculture industry. Atrazine (ATZ) is a chlorinated triazine herbicide that consists of a ring structure, known as the triazine ring, along with a chlorine atom and five nitrogen atoms. ATZ is a water-soluble herbicide, which makes it capable of easily infiltrating into majority of the aquatic ecosystems. There are reports of toxic effects of ATZ on different systems of the body but, unfortunately, majority of these scientific reports were documented in animals. The herbicide was reported to enter the body through various routes. The toxicity of the herbicide can cause deleterious effects on the respiratory, reproductive, endocrine, central nervous system, gastrointestinal, and urinary systems of the human body. Alarmingly, few studies in industrial workers showed ATZ exposure leading to cancer. We embarked on the present review to discuss the mechanism of action of ATZ toxicity for which there is no specific antidote or drug. Evidence-based published literature on the effective use of natural products such as lycopene, curcumin, Panax ginseng, Spirulina platensis, Fucoidans, vitamin C, soyabeans, quercetin, L-carnitine, Telfairia occidentalis, vitamin E, Garcinia kola, melatonin, selenium, Isatis indigotica, polyphenols, Acacia nilotica, and Zingiber officinale were discussed in detail. In the absence of any particular allopathic drug, the present review may open the doors for future drug design involving the natural products and their active compounds. Full article

Trypanosomiasis is a serious disease that affects both humans and animals, causing social and economic losses. Efforts to find new therapeutic approaches are warranted to improve treatment options. Therefore, the purpose of this communication includes the phytochemical screening of a methanolic extract of Garcinia kola nuts and the in vivo evaluation of its biological activity against rats infected with Trypanosoma brucei brucei and treated with 4 different concentrations of the extract (0.01, 0.1, 1, and 10 mg/kg). Treatment with suramin served as a positive control, while the negative control received no drug. Since the general toxicity of the extract could be ruled out, efficacy was evaluated based on physiological changes, such as induction of trypanosome parasitemia, influence on body temperature, and body weight. Survival was assessed during this study. Physical parameters, behavioral characteristics, and various hematological indices were also monitored. Based on the (patho)physiological and behavioral parameters (e.g., no parasitemia, no increase in body temperature, an increase in body weight, no loss of condition, no alopecia, and no gangrene), the efficacy of the extract was evident, which was also confirmed by 100% survival, while in the negative control, all rats died during the observation period. Since overall very similar results were obtained as a result of treatment with the established suramin, the in vivo antitrypanosomal activity of a methanolic extract of G. kola nuts on rats can be demonstrated in this communication. This opens the way, for example, for further development of drug formulations based on this methanolic extract. Full article

Seeds and bark of Garcinia kola Heckel (Clusiaceae) are popular products in West and Central Africa. Despite the tree’s economic and cultural importance, little is known about its phenotypic and genotypic variation. This study characterised the morphological and genetic diversity of G. kola in South Cameroon, searching for traits and populations that might be used for domestication. Morphological assessment and amplified fragment length polymorphism (AFLP) markers were applied to characterise diversity among geographic populations from Central and South regions, and between managed and wild trees. AFLP-SURV and analysis of molecular variance results indicated that a major part of genetic diversity is harboured within populations rather than between them. Bayesian analysis, principal component analysis and t-SNE identified three clusters where Ebolowa emerged as the transition population combining features from both regions. Trees from the South demonstrated a higher incidence of domestication-related traits, showing higher genetic diversity compared to the Central region. This suggests that individuals from the South might be more suitable for selection as “elite trees” in future breeding strategies for the species. No significant differences in phenotype and genotype were revealed between wild and managed populations, suggesting G. kola is still in the early stages of its domestication process. Full article

Garcinia biflavonoid 1 (GB1) is one of the active chemical components of Garcinia kola and is reported to be capable of reducing the intracellular lipid deposition, which is the most significant characteristic of non-alcoholic fatty liver disease. However, its bioactive mechanism remains elusive. In the current study, the lipid deposition was induced in HepG2 cells by exposure to oleic acid and palmitic acid (OA&PA), then the effect of GB1 on lipid metabolism and oxidative stress and the role of regulating PPARα in these cells was investigated. We found that GB1 could ameliorate the lipid deposition by reducing triglycerides (TGs) and upregulate the expression of PPARα and SIRT6, suppressing the cell apoptosis by reducing the oxidative stress and the inflammatory factors of ROS, IL10, and TNFα. The mechanism study showed that GB1 had bioactivity in a PPARα-dependent manner based on its failing to improve the lipid deposition and oxidative stress in PPARα-deficient cells. The result revealed that GB1 had significant bioactivity on improving the lipid metabolism, and its potential primary action mechanism suggested that GB1 could be a potential candidate for management of non-alcoholic fatty liver disease. Full article

To achieve sustainability, it is necessary to use proper treatment methods to reduce the pollutant loads of receiving waters. This study investigates the coagulative reduction of turbidity, COD, BOD and colour from aquaculture wastewater (AW) using a novel Garcinia kola seeds coagulant (GKC). This coagulant was obtained from extraction of Garcinia kola seeds and analysed for its spectral and morphological characteristics through FTIR and SEM. The kinetics of coagulation-flocculation were also investigated in terms of total dissolved and suspended solids (TDSP). The seeds had 11.27% protein and 68.33% carbohydrate, showing usability in adsorption/charges neutralisation as a coagulant to reduce particles. Maximal turbidity reduction = 81.93%, COD = 75.03%, BOD = 72.84% and colour = 56.69% at 0.3 g GKC/L, pH 2, 60 min and 303 K were achieved. Von Smoluchowski’s second-order peri-kinetics theory was used to fit the results, giving R² > 0.9. At a coagulation order (α) of 2, the reaction rate (K_C) and half-life (${\tau }_{S1/2}$) were 0.0003 L/g·min and 25.3 min at the optimal conditions. The sorption data better fit the Lagergren compared to the Ho adsorption model. Furthermore, the net cost of using GKC to handle 1 L of AW (including electricity and material costs) was calculated to be 1.57 EUR, and the costs of 0.3 g/L GKC preparation and energy were 0.27 and 1.30 EUR, respectively. In summary, these seeds can be used to pre-treat AW. Full article

The rate of landcover change linked to deforestation and forest degradation in tropical environments has continued to surge despite a series of forest governance policy instruments over the years. These informed the launch of one of the most important international policies called Reducing Emission from Deforestation and Forest Degradation Plus (REDD+) to combat forest destruction. REDD+ assumes that communities will have increased assets to natural capital which will enhance their livelihood portfolio and mitigate the effects of climate variability and change across biomes. The aim of this study is to ascertain the livelihoods impacts of forest carbon protection within the context of REDD+ in Cross River State, Nigeria. Six forest communities were chosen across three agroecological zones of the State. Anchored on the Sustainable Livelihood Framework, a set of questionnaires were administered to randomly picked households. The results indicate that more than half of the respondents aligned with financial payment and more natural resources as the perceived benefits of carbon protection. More so, a multinomial logistic regression showed that income was the main factor that influenced respondent’s support for forest carbon protection. Analysis of income trends from the ‘big seven’ non-timber forest resources in the region showed increase in Gnetum africanum, Bushmeat, Irvingia gabonensis, Garcinia kola, while carpolobia spp., Randia and rattan cane revealed declining income since inception of REDD+. The recorded increase in household income was attributed to a ban in logging. It is recommended that the forest communities should be more heavily involved in the subsequent phases of the project implementation to avoid carbon leakages. Full article

In a tropical rainforest environment, different storage strategies are often adopted in the preservation of primary processed food crops, such as maize, sorghum, etc., after drying and dehulling to increase shelf-life. For breadfruit seeds (Treculia Africana), the current challenge is identifying the most appropriate short-term storage and packaging methods that can retain the quality of stored products and extend shelf-life. In this regard, we compared the performance of a plastic container, a weaved silo bag and a locally developed silo bin for the short-term storage of parboiled, dehulled and dried breadfruit seeds treated with locally sourced and affordable alligator pepper (Zingiberaceaeaframomum melegueta) and bitter kola (garcinia) powder as preservatives. We show that the concentration of CO₂ was lower in the silo bin treated with 150 g alligator pepper and higher in the silo bag-treated with 100 g bitter kola nut. A higher CO₂ concentration resulted in limited oxygen availability, higher water vapor, and a higher heat release rate. Non-treated bag storage had the highest average mold count of 1.093 × 10³ CFU/mL, while silo bin-stored breadfruit treated with 150 g of alligator pepper had the lowest mold count of 2.6 × 10² CFU/mL. The storage time and botanical treatments influenced both the crude protein and crude fiber content. Average insect infestations were low (0–4.5) in the silo bin with breadfruits treated with alligator pepper powder, as the seeds seemed to continue to desorb moisture in storage, unlike in other treatments. The obtained results revealed the high potential of alligator pepper (Zingiberaceaeaframomum melegueta) as a botanical insecticide in preventing insect infestation and mold growth in stored breadfruit instead of using synthetic insecticide. An aluminum silo bin with alligator pepper powder is recommended to store dried and dehulled breadfruit seeds as a baseline for other tropical crops. Full article

Approximately 400 Garcinia species are distributed around the world. Previous studies have reported the extracts from bark, seed, fruits, peels, leaves, and stems of Garcinia mangostana, G. xanthochymus, and G. cambogia that were used to treat adipogenesis, inflammation, obesity, cancer, cardiovascular diseases, and diabetes. Moreover, the hypoglycemic effects and underlined actions of different species such as G. kola, G. pedunculata, and G. prainiana have been elucidated. However, the anti-hyperglycemia of G. linii remains to be verified in this aspect. In this article, the published literature was collected and reviewed based on the medicinal characteristics of the species Garcinia, particularly in diabetic care to deliberate the known constituents from Garcinia and further focus on and isolate new compounds of G. linii (Taiwan distinctive species) on various hypoglycemic targets including α-amylase, α-glucosidase, 5′-adenosine monophosphate-activated protein kinase (AMPK), insulin receptor kinase, peroxisome proliferator-activated receptor gamma (PPARγ), and dipeptidyl peptidase-4 (DPP-4) via the molecular docking approach with Gold program to explore the potential candidates for anti-diabetic treatments. Accordingly, benzopyrans and triterpenes are postulated to be the active components in G. linii for mediating blood glucose. To further validate the potency of those active components, in vitro enzymatic and cellular function assays with in vivo animal efficacy experiments need to be performed in the near future. Full article

Drug-resistant-diarrhoeagenic bacteria are currently emerging healthcare challenge. This study investigated the effects of Vernonia amygdalina, Garcinia kola, tetracycline and metronidazole combinations on such bacteria. Agar well diffusion method was employed to determine the inhibitory effects of the herbal extracts on diarrhoeagenic bacteria while Time-Kill Assay was used to determine bactericidal effects of the extracts against test isolates. Interactions between plant extracts and antibiotics were investigated using Checkerboard assay. Minimum inhibitory concentrations of the extracts against the bacterial isolates ranged between 3.125–50 mg/mL, while those of tetracycline and metronidazole ranged from 30–50 μg/mL. Synergism was observed against B. cereus and S. aureus for metronidazole + aqueous G. kola at all ratios. Generally, the combinations aqueous G. kola + ethanolic G. kola and aqueous G. kola + ethanolic V. amygdalina showed more pronounced synergism against the Staphylococcus aureus than B. cereus isolates with the fractional inhibition concentration (FIC) indices ranging from 0.32–0.95. Synergism of tetracycline + crude extracts and metronidazole combinations were more pronounced on the test isolates and especially on the Gram-negative organisms with FIC indices ranging from 0.41–0.91. Conclusion: The herbal extracts combinations and extracts–antibiotics combinations are synergistic on diarrhoeagenic bacteria at defined combination ratios. Full article

Garcinia kola Heckel (Clusiaceae), known as bitter kola, is a multipurpose tree indigenous to West and Central Africa. This highly preferred species is called “wonder plant” because all of its parts can be used as medicine. Its seeds, the most valued product of the tree, are commonly eaten to prevent/cure gastric disorders and for their typical astringent taste. There is a vast evidence that bioactive components of the seeds can serve as alternative medicine to treat/prevent severe illnesses such as malaria, hepatitis and immune-destructive diseases. Despite the species’ pharmaceutical potential and its high preference by West and Central African communities, G. kola is still at the beginning of its domestication process. Even though, there are numerous scientific articles published on species‘ biological activities, it is a difficult task to find basic information on its diversity, distribution, genetics, silvicultural management or botany. Therefore, in this very first review published on G. kola, we summarize all relevant information known about the species, target some of the challenges connected with its cultivation and propose a leading direction for future research and domestication process. Full article

We assessed the bioactivity of G. kola seeds on Streptococcus pyogenes, Staphylococcus aureus, Plesiomonas shigelloides and Salmonella typhimurium. The crude ethyl acetate, ethanol, methanol, acetone and aqueous extracts were screened by the agar-well diffusion method and their activities were further determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays. The extracts were fractionated by Thin Layer Chromatography (TLC). Bioautography was used to assess the activity of the possible classes of compounds present in the more active extracts. Column chromatography was used to purify the active compounds from the mixture, while GC-MS was used to identify the phytocomponents of the fractions. The inhibition zone diameters of the extracts ranged from 0–24 ± 1.1 mm, while MIC and MBC values ranged between 0.04–1.25 mg/mL and 0.081–2.5 mg/mL, respectively. The chloroform/ethyl acetate/formic acid (CEF) solvent system separated more active compounds. The MIC of the fractions ranged between 0.0006–2.5 mg/mL. CEF 3 (F3), CEF 11 (F11) and CEF 12 (F12) revealed the presence of high levels of linoleic acid, 1,2-benzenedicarboxylic acid and 2,3-dihydro-3,5-dihydroxy-6-methyl ester, respectively. The results obtained from this study justify the use of this plant in traditional medicine and provide leads which could be further exploited for the development of new and potent antimicrobials. Full article

We assessed the anti-Listerial activities of crude n-hexane and aqueous extracts of Garcinia kola seeds against a panel of 42 Listeria isolates previously isolated from wastewater effluents in the Eastern Cape Province of South Africa and belonging to Listeria monocytogenes, Listeria grayi and Listeria ivanovii species. The n-hexane fraction was active against 45% of the test bacteria with zones of inhibition ranging between 8–17 mm, while the aqueous fraction was active against 29% with zones of inhibition ranging between 8–11 mm. The minimum inhibitory concentrations (MIC) were within the ranges of 0.079–0.625 mg/mL for the n-hexane extract and 10 to >10 mg/mL for the aqueous extract. The rate of kill experiment carried out for the n-hexane extract only, revealed complete elimination of the initial bacterial population for L. grayi (LAL 15) at 3× and 4× MIC after 90 and 60 min; L. monocytogenes (LAL 8) at 3× and 4× MIC after 60 and 15 min; L. ivanovii (LEL 18) at 3× and 4× MIC after 120 and 15 min; L. ivanovii (LEL 30) at 2, 3 and 4× MIC values after 105, 90 and 15 min exposure time respectively. The rate of kill activities were time- and concentration-dependant and the extract proved to be bactericidal as it achieved a more than 3log₁₀ decrease in viable cell counts after 2 h exposure time for all of the four test organisms at 3× and 4× MIC values. The results therefore show the potential presence of anti-Listerial compounds in Garcinia kola seeds that can be exploited in effective anti-Listerial chemotherapy. Full article

The aim of this study was to identify sources of cheap starting materials for the synthesis of new drugs against Helicobacter pylori. Solvent-extracts of selected medicinal plants; Combretum molle, Sclerocarya birrea, Garcinia kola, Alepidea amatymbica and a single Strychnos species were investigated against 30 clinical strains of H. pylori alongside a reference control strain (NCTC 11638) using standard microbiological techniques. Metronidazole and amoxicillin were included in these experiments as positive control antibiotics. All the plants demonstrated anti-H. pylori activity with zone diameters of inhibition between 0 and 38 mm and 50% minimum inhibitory concentration (MIC₅₀) values ranging from 0.06 to 5.0 mg/mL. MIC₅₀ values for amoxicillin and metronidazole ranged from 0.001 to 0.63 mg/mL and 0.004 to 5.0 mg/mL respectively. The acetone extracts of C. molle and S. birrea exhibited a remarkable bactericidal activity against H. pylori killing more than 50% of the strains within 18 h at 4× MIC and complete elimination of the organisms within 24 h. Their antimicrobial activity was comparable to the control antibiotics. However, the activity of the ethanol extract of G. kola was lower than amoxicillin (P < 0.05) as opposed to metronidazole (P > 0.05). These results demonstrate that S. birrea, C. molle and G. kola may represent good sources of compounds with anti-H. pylori activity. Full article