Search Results (212)

Soybean (Glycine max) is a protein-rich legume crop that plays an important role in achieving food security. The aim of this study was to isolate soybean-nodulating rhizobia from Côte d’Ivoire soils and evaluate their potential as efficient strains in order to develop local bioinoculants. For this objective, 38 composite soil samples were collected from Côte d’Ivoire’s five major climatic zones. These soils were used as substrate to trap the nodulating rhizobia using the promiscuous soybean variety R2-231. A total of 110 bacterial strains were isolated and subsequently identified. The analysis of ITS (rDNA16S-23S), glnII and recA sequences revealed a relatively low genetic diversity of these native rhizobia. Moreover, the ITS phylogeny showed that these were scattered into two Bradyrhizobium clades dominated by the B. elkanii supergroup, with ca. 75% of all isolates. Concatenated glnII-recA sequence phylogeny confirmed that the isolates belong in the majority to ‘B. brasilense’, together with B. vignae and some putative genospecies of Bradyrhizobium that needs further elucidation. The core gene phylogeny was found to be incongruent with nodC and nifH phylogenies, probably due to lateral gene transfer influence on the symbiotic genes. The diversity and composition of the Bradyrhizobium species varied significantly among different sampling sites, and the key explanatory variables identified were carbon (C), magnesium (Mg), nitrogen (N), pH, and annual precipitation. Based on both shoot biomass and leaf relative chlorophyll content, three isolates consistently showed a higher symbiotic effectiveness than the exotic inoculant strain Bradyrhizobium IRAT-FA3, demonstrating their potential to serve as indigenous elite strains as bioinoculants. Full article

Neisseria gonorrhoeae is the causative agent of the sexually transmitted infection gonorrhea. Preventative vaccines or novel treatments based on a better understanding of the molecular basis of N. gonorrhoeae infection are required as resistance to current antibiotics is widespread. Toxin–antitoxin (TA) systems modulate bacterial physiology by interfering with vital cellular processes; type II TA systems, where both toxin and antitoxin are proteins, are the best-studied. Bioinformatics analysis revealed genes encoding an uncharacterized type II HicAB TA system in the N. gonorrhoeae strain FA1090 chromosome, which were also present in >83% of the other gonococcal genome sequences examined. Gonococcal HicA overproduction inhibited bacterial growth in Escherichia coli, an effect that could be counteracted by the co-expression of HicB. Kill/rescue assays showed that this effect was bacteriostatic rather than bactericidal. The site-directed mutagenesis of key histidine and glycine residues (Gly22, His24, His29) abolished HicA-mediated growth arrest. N. gonorrhoeae FA1090∆hicAB and complemented derivatives that expressed IPTG-inducible hicA, hicB, or hicAB, respectively, grew as wild type, except for IPTG-induced FA1090∆hicAB::hicA. RT-PCR demonstrated that hicAB are transcribed in vitro under the culture conditions used. The deletion of hicAB had no effect on biofilm formation. Our study describes the first characterization of a HicAB TA system in N. gonorrhoeae. Full article

Fatty fish, such as mackerel (Scomber scombrus), are recommended as part of a healthy diet, providing essential fatty acids (FA). Fried fish is appreciated for its attributes, including a crispy texture, golden crust, and pleasant taste. However, frying increases the fat content and the caloric value of food. This study evaluated the use of pre-frying edible coatings on mackerel fillets aiming to: (i) reduce oil absorption, (ii) minimize water loss, preserving fish succulence, and (iii) prevent fat oxidation. For this purpose, alginate- and carrageenan-based coatings were supplemented with extracts of Pelvetia canaliculata (Pc), a seaweed with high potential as a source of bioactive compounds. The fried fillets were analysed for colour, texture, moisture, ash, lipid content, and FA profile. No significant differences were observed for colour and textural parameters. Fillets coated with Pc-supplemented carrageenan showed the highest moisture (an increase of 3%) and the lowest fat content (a decrease of 7,5%) compared to the control (fried uncoated fillets). Coated fillets also exhibited reduced saturated FA and increased monounsaturated FA. In general, linoleic acid (C18:2) decreased markedly, while the values for docosahexaenoic acid (C22:6, n-3) remained stable (11–12% of total FA). Moreover, the n3/n6 ratio and atherogenic indices (AI) were improved in the coated fillets. Full article

Alkali-activated binders (AAB) are a suitable and sustainable alternative to ordinary Portland cement (OPC), with reductions in natural resource usage and environmental emissions in regions where the necessary industrial residues are available. Despite its potential, the lack of mix design methods still limits its applications. This paper proposes a systematic parametric validation for AAB mix design applied to pastes and concretes, valorizing steel slag as precursors. The composed binders are based on coal fly ash (FA) and Basic Oxygen Furnace (BOF) steel slag. These precursors were activated with sodium silicate (Na₂SiO₃) and sodium hydroxide (NaOH) alkaline solutions. A parametric investigation was performed on the mix design parameters, sweeping the (i) alkali content from 6% to 10%, (ii) silica modulus (SiO₂/Na₂O) from 0.75 to 1.75, and (iii) ash-to-slag ratios in the proportions of 75:25 and 50:50, using parametric intervals retrieved from the literature. These variations were analyzed using response surface methodology (RSM) to develop a mechanical model of the compressive strength of the hardened paste. Flowability, yield stress, and setting time were evaluated. Statistical analyses, ANOVA and the Duncan test, validated the model and identified interactions between variables. The concrete formulation design was based on aggregates packing analysis with different paste contents (from 32% up to 38.4%), aiming at self-compacting concrete (SCC) with slump flow class 1 (SF1). The influence of the curing condition was evaluated, varying with ambient and thermal conditions, at 25 °C and 65 °C, respectively, for the initial 24 h. The results showed that lower silica modulus (0.75) achieved the highest compressive strength at 80.1 MPa (28 d) for pastes compressive strength, densifying the composite matrix. The concrete application of the binder achieved SF1 fluidity, with 575 mm spread, 64.1 MPa of compressive strength, and 26.2 GPa of Young’s modulus in thermal cure conditions. These findings demonstrate the potential for developing sustainable high-performance materials based on parametric design of AAB formulations and mix design. Full article

Monolepta hieroglyphica Motschulsky, a major agricultural pest in China, causes considerable economic damage to crops, such as maize. In this study, a Bacillus thuringiensis (Bt) strain was discovered to exhibit insecticidal activity against M. hieroglyphica. A novel Bt gene, vpb4Fa1, with toxicity against both adults and larvae of M. hieroglyphica was cloned. The Vpb4Fa1 protein causes damage to the midgut of adult M. hieroglyphica, disrupting their normal growth and development and ultimately leading to death. To further enhance the insecticidal activity of the vpb4Fa1 gene, a random mutation library was established. A total of 75 mutants with amino acid mutations were generated, among which 7 mutants demonstrated significantly enhanced activity relative to the wild-type gene. Notably, three mutants, C9, 6C2, and 6A7, exhibited the highest activity, with LC₅₀ values for adult M. hieroglyphica of 10.21, 9.45, and 9.83 µg/g, respectively. The mutants C9, 6C2, and 6A7 each harbored nine, three, and six amino acid mutations, respectively, mainly located in Domains I, II, and III. The novel insecticidal gene vpb4Fa1 and its mutants offer valuable genetic resources for the biological control of M. hieroglyphica and the development of Bt transgenic maize. Full article

This study explores the determinants of financial literacy (FL) and the relationship between FL, financial inclusion (FI), and financial well-being (FW-B) in the Ecuadorian banking industry. It also tests the moderating role of digital literacy (DL) on the relationship between FL-FI, FL-FW-B, and FI-FW-B. This study employs a self-designed online questionnaire with a structural equation model to prove the relationship between variables. Among 321 collected data, the final valid dataset consisted of 294 registers. The main findings revealed that (i) financial behavior (FB), financial attitudes (FA), and financial skills (FS) have a significant and positive influence over FL, (ii) FL positively affects FI and FW-B, (iii) FI has a positive and significant relationship with FW-B, and (iv) DL does not moderate the relationship between variables, given DL depends on socio-economic factors (especially educational aspects) and the degree of technology and innovation adopted by digital banking customers. Study results are aligned with previous studies in the United States, India, Greece, and Finland. This study contributes to the research by offering a complete view of the importance of transcendence of the presence of FL in educational programs to improve the FI, financial development, and FW-B of banking customers. The study limitation is the absence of the FL index in the Ecuadorian environment. For future research, the study recommends performing a longitudinal study of FL and FI and including different statuses and categories to test the econometric model. Full article

Integrin-linked kinase (ILK) is a key scaffolding protein between extracellular matrix protein and the cytoskeleton and has been implicated previously in the pathogenesis of renal damage. However, its involvement in renal mitochondrial dysfunction remains to be elucidated. We studied the role of ILK and its downstream regulations in renal damage and mitochondria function both in vivo and vitro, using a folic acid (FA)-induced kidney disease model. Wild type (WT) and ILK conditional-knockdown (cKD-ILK) mice were injected with a single intraperitoneal dose of FA and studied after 15 days of chronic renal damage progression. Human Kidney tubular epithelial cells (HK2) were transfected with specific siRNAs targeting ILK, glycogen synthase kinase 3-β (GSK3β), or CCAAT/enhancer binding protein-β (C/EBPβ). The expressions and activities of renal ILK, GSK3β, C/EBPβ, mitochondrial oxidative phosphorylation enzymes, and mitochondrial membrane potential were assessed. Additionally, the expression of markers for fibrosis fibronectin (FN) and collagen 1 (COL1A1), for autophagy p62 and cytosolic light chain 3 (LC3B) isoforms II and I, and mitochondrial homeostasis marker carnitine palmitoyl-transferase 1A (CPT1A) were evaluated using immunoblotting, RT-qPCR, immunofluorescence, or colorimetric assays. FA upregulated ILK expression, leading to the decrease of GSK3β activity, increased tubular fibrosis, and produced mitochondrial dysfunction, both in vivo and vitro. These alterations were fully or partially reversed upon ILK depletion, mitigating FA-induced renal damage. The signaling axis composed by ILK, GSK3β, and C/EBPβ regulated CPT1A transcription as the limiting factor in the FA-based impaired mitochondrial activity. We highlight ILK as a potential therapeutical target for preserving mitochondrial function in kidney injury. Full article

Introduction: Obstructive Sleep Apnea Syndrome (OSAS) is a sleep-related breathing disorder common in children, often linked to craniofacial anomalies like retrognathic mandibles in Class II malocclusions. Functional appliances (FAs) have been proposed as non-invasive treatments to improve OSAS symptoms by modifying upper airway dimensions. Objective: this systematic review evaluates the effectiveness of functional appliances in improving upper airway structures in children with OSAS. Materials and Methods: the study was conducted according to PRISMA guidelines, analyzing studies published between 2004 and 2024 on PubMed, Scopus, Web of Science, Embase, and Cochrane Library databases. Inclusion criteria focused on growing patients (≤14 years) with OSAS and Class II skeletal malocclusions treated with FAs. Results: Of 1298 articles screened, four studies met the inclusion criteria. All studies reported a significant increase in upper airway dimensions of approximately 21% after treatment with FAs. Specifically, the cephalometric and tomographic evaluations revealed a clear enlargement of the superior posterior airway space of about 0.5 mm and a consequent improvement of the respiratory function. Discrepancies arose regarding changes in soft palate length and hyoid bone position, perhaps due to the measurement methods’ variation. Conclusions: Functional appliances appear effective in improving upper airway dimensions and alleviating OSAS symptoms in children. However, the limited number of studies, small sample sizes, and short follow-up periods emphasize the need for further research to confirm long-term efficacy and standardize evaluation protocols. Full article

Transition-metal ion copper(II) (Cu(II)) has drawn increasing attention as a small-molecular cancer theranostic agent. However, delivering a sufficient dosage of Cu(II) to the tumor site and integrating multiple imaging modalities to achieve precise and effective cancer theranostics remains a critical challenge. Herein, an emerging Cu(II)-based nanocomposite has been synthesized for targeted tumor computed tomography (CT)/magnetic resonance (MR) dual-mode imaging and chemodynamic therapy (CDT). Briefly, 2-picolinic acid (PA-COOH), polyethylene glycol (PEG)-linked folic acid (FA), and fluorescein isothiocyanate (FI) were sequentially conjugated with polyethylenimine (PEI.NH₂) and then in situ fabrication of gold nanoparticles (Au NPs) occurred within the PEI.NH₂ internal cavity. After acetylation of PEI.NH₂ terminal amines and Cu(II) complexation, the Cu(II)-based nanocomposites FA-Au/Cu(II) PENPs with a mean diameter of 2.87 nm were generated. The synthesized FA-Au/Cu(II) PENPs showed favorable stability of colloidal dispersion, sustainable Cu(II) release properties in a pH-dependent manner, and Fenton-like catalytic activity specifically. With the FA-mediated targeting pathway, FA-Au/Cu(II) PENPs can specifically accumulate in cancer cells with high expression of FA receptors. Meanwhile, the complementary CT/MR dual-mode imaging in vitro and in vivo can be afforded by FA-Au/Cu(II) PENPs based on the excellent X-ray attenuation properties of Au NPs and the applicable r₁ relaxivity (0.7378 mM⁻¹s⁻¹) of Cu(II). Notably, the Cu(II)-mediated CDT mechanism enables FA-Au/Cu(II) PENPs to elicit the generation of toxic hydroxyl radicals (·OH), depletion of glutathione (GSH), promotion of lipid peroxidation (LPO), and induction of cancer cell apoptosis in vitro, and further demonstrates remarkable anti-tumor efficacy in a xenograft tumor model. With the illustrated targeted theranostic capacity of FA-Au/Cu(II) PENPs towards tumors, this Cu(II)-based nanocomposite paradigm inspires the construction of advanced theranostic nanoplatforms incorporating alternative transition metal ions. Full article

Montbeliarde dairy cattle were introduced in Ecuador with the aim of ameliorating the production performance of other cattle breeds. The aim was to analyze the reproductive performance, demographic structure, inbreeding, and genetic diversity of Montbeliarde cattle obtained by absorption crossing. Official pedigree records from the Ecuadorian Montbeliarde Association were used. The total population was divided into seven populations: (i) historical (all individuals), (ii) 1999–2003, (iii) 2004–2008, (iv) 2009–2013, (v) 2014–2018, (vi) 2019–2023 (current), and (vii) reference (individuals with known parents). Demographic structure variables analyzed: pedigree completeness index (PCI), number of equivalents (GEqu), complete (GCom) and maximum (GMax) generations, and generation interval (GI). Inbreeding-derived variables analyzed: inbreeding (F), inbreeding increment (ΔF), average relatedness (AR), co-ancestry (C), non-random mating (α), effective population size (Ne), and genetic conservation index (GCI). Gene origin probability/genetic diversity variables analyzed: number of founders (f), effective number of founders (fe) and ancestors (fa), number of equivalent genomes (fg), fe/fa and fg/fa ratio, and DG losses. The databases were analyzed by ENDOG, POPREP, and CPC software. PCI in the historical population compared to the current population decreased from 72.95 to 65.87% (sire/dam pathway), while the GI decreased from 7.17 to 3.08 years when the historical and current populations were compared. Dam reproductive efficiency increased over time. Moreover, F = ~1%, AR = 2.06%, ΔF = 0.22%, C = 1.03%, α = −0.0077, GCI = 3.12, and Ne = 58 values were obtained. Gene origin probability in the reference population was f = 439, fa = 37, fe = 71.64, fg = 23.43, and fg/fe = 0.33, showing a GD loss due to unequal contribution of founders (0.55%) and bottleneck and genetic drift (4.25%). In conclusion, the Ecuadorian Montbeliarde cattle population displayed a relatively low diversity and high genetic relationship. Inbreeding levels increased while Ne values decreased over time. The introduction of new purebred bloodlines could be important to minimize the inbreeding levels ensuring the long-term conservation of this breed and minimizing GD loss. Full article

Two Fe-based hybrids, [SiO₂@NP(Ph)₂/Fe^II/PP₃] and [SiO₂@NP(t-Bu)₂/Fe^II/PP₃], were synthesized using the double-ligand approach by covalently grafting NP ligands onto the surface of SiO₂. Both catalytic systems were evaluated for H₂ production through formic acid dehydrogenation (FADH), revealing important efficiency without requiring additional additives and/or co-catalysts. During the continuous addition of FA, [SiO₂@NP(Ph)₂/Fe^II/PP₃] and [SiO₂@NP(t-Bu)₂/Fe^II/PP₃] demonstrated great stability, achieving total TONs = 20,636 and 20,854, respectively. FT-IR and Raman spectroscopy provided insights into the role of NP ligands, such as NP(Ph)₂ and NP(t-Bu)₂, on the assembly and structural configuration of active hybrid Fe catalysts and their ability to dehydrogenate formic acid. Additional studies, including in situ mapping of the solution potential (E_h) of the catalytic reaction and an Arrhenius study of the activation energy (E_a), revealed a correlation between E_a and H₂ production rates: the system [SiO₂@NP(Ph)₂/Fe^II/PP₃] with an E_a = 29.4 KJ/mol shows an H₂ production rate of 58 mL-H₂/min, while [SiO₂@NP(t-Bu)₂/Fe^II/PP₃] with a E_a = 50.6 KJ/mol shows an H₂ production rate of 55 mL-H₂/min. This is the first example of a heterogeneous FADH system where the original strategy of a “double-ligand” has been demonstrated for homogeneous FADH catalytic systems. Herein we demonstrate that we can engineer a decrease in the activation barrier Ea via two synergistic steps: (i) via grafting of the NP ligand onto SiO₂ and (ii) using PP₃ as double ligand. This strategy leads to a boost in the H₂ production efficiency of [SiO₂@NP(Ph)₂/Fe^II/PP₃] as a heterogeneous catalyst, which for the first time has been shown to be able to outperform the parental reference/homogenous catalyst [Fe^II/PP₃]. Full article

Water and its management have played a pivotal role in the evolution of organisms and civilizations, fulfilling essential roles in personal use, industry, irrigation, and drinking from ancient times to the present. This study seeks to evaluate groundwater quality for irrigation and drinking in the Northern Peloponnese region, specifically the wells of Loutraki and Schinos areas and the springs of the Gerania Mountains (Mts.), using geo-environmental indices and ionic ratios. For the first time, geo-environmental indices have been applied to a region where groundwater serves multiple purposes, addressing the challenge of understanding their dynamics to optimize their application in environmental science and groundwater pollution research. To achieve this, 68 groundwater samples from the study area were utilized, and a total of 25 geo-environmental indices were calculated to assess water quality. These indices examined: (i) drinking suitability (NPI, RI, PIG, WQI, and WPI), (ii) irrigation suitability (SAR, KR, %Na, PS, MAR, RSC, SSP, TH, PI, IWQI, and TDS), (iii) potentially toxic element (PTE) loadings (Cd, HEI, and HPI), and (iv) major hydrogeochemical processes, expressed as ionic ratios (Ca/Mg, Ca/SO₄, Ca/Na, Cl/NO₃, Cl/HCO₃, and Si/NO₃). Data processing involved descriptive statistics, hydrogeochemical bivariate plots, Spearman correlation coefficients, and multivariate statistical analyses, including factor analysis (FA) and R-mode hierarchical cluster analysis (HCA). Results revealed that all groundwater samples (100%) from the Loutraki area and the Gerania Mts. were of good quality for both drinking and irrigation purposes. In contrast, groundwater from the Schinos area exhibited lower quality, with most samples (93.9%) considered suitable only for irrigation. The deterioration in the coastal aquifer of the Schinos area is attributed to elevated concentrations of Cl⁻, Na⁺, NO_3⁻, As, and Cr resulting from salinization and relatively limited anthropogenic influences. The study highlights that relying on individual geo-environmental indices can yield misleading results due to their dependence on factors such as researcher expertise, methodological choices, and the indices’ inherent limitations. Consequently, this research emphasizes the necessity of combining indices to enhance the reliability, accuracy, and robustness of groundwater quality assessments and hydrogeochemical evaluations. Last but not least, the findings demonstrate that calculating all available geo-environmental indices is unnecessary. Instead, selecting a subset of indices that either reflect the impact of specific elemental concentrations or can be effectively integrated with others is sufficient. This streamlined approach addresses challenges in optimizing geo-environmental index applications and contributes to improved groundwater resource management. Full article

Background/Objectives: Salter–Harris II (SH-II) distal tibia fractures are the most common physeal ankle fractures in children; however, indications for surgical management remain controversial, and patient-reported outcomes for different management strategies are unknown. The purpose of the current study is to compare differences in clinical and patient-reported outcomes following operative and non-operative management of this injury. Methods: We performed a retrospective cohort study of pediatric patients who were treated at a single institution for SH-II distal tibia fractures between 2013 and 2020. Variables included age, gender, operative versus non-operative treatment, and premature physeal closure (PPC). Patients were also contacted for patient-reported outcome scores (PROs), which included the visual analog scale foot and ankle (VAS-FA) and the PROMIS pediatric mobility instrument obtained at a minimum of 2 years post-injury. Results: Demographic and clinical information was obtained for 46 patients. Our cohort was 52% male with mean age of 11.9 years at injury. At 6 months, the rate of PPC in our cohort was 25%, with no differences between operative and non-operative patients (29% vs. 24%, p = 0.80). A total of 15 of the 46 patients provided PROs, with an average follow-up time of 5.1 years (range: 2.9–9.1). VAS-FA and PROMIS pediatric mobility scores were similar between operative and non-operative patients. Conclusions: This pilot study suggests no differences in PROs following operative and non-operative management for SH-II distal tibia fractures; however, future studies with larger cohort sizes and longer follow-up times are needed to further examine these outcomes. Full article

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by self-antibody production and widespread inflammation affecting various body tissues. This disease is driven by the breakdown of immune tolerance, which promotes the activation of autoreactive B and T cells. A key feature of SLE is dysregulation in antigen presentation, where antigen-presenting cells (APCs) play a central role in perpetuating immune responses. Dendritic cells (DCs) are highly specialized for antigen presentation among APCs. At the same time, myeloid-derived suppressor cells (MDSCs) can also express MHC-II molecules, although their role in SLE is less understood. Utilizing the SLE model, MRL/MpJ-Fas^lpr/J, we determined the presence of different phenotypes of DCs and MDSCs expressing MHC-II in secondary lymphoid organs, along with the gene expression of ICOSL, CD80 and CD86 in the spleen. Our study determined that the most abundant population of APCs in secondary lymphoid organs corresponds to cDC CD103⁻CD11b⁺ MHC-II⁺ throughout SLE development. Additionally, ICOSL expression increased over time, becoming more preponderant in week 16 in the SLE model, which could indicate that it is a crucial pathway for the development and progression of the pathology. In week 16, we observed a positive correlation between M-MDSC MHC-II and IFN-γ-producing CD4⁺ T cells. Full article

Background: Stroke ranks as the second leading cause of death and the third leading cause of disability in adults worldwide. While an unhealthy diet is an independent risk factor for stroke, its association with disordered eating behaviours on stroke remains overlooked. This exploratory study aimed to evaluate the prevalence and severity of addictive-like eating behaviours in stroke patients and their association with the main vascular stroke risk factors. Methods: First-ever minor or moderate stroke patients with the ability to complete self-report questionnaires were included. Addictive-like eating was assessed using the Yale Food Addiction Scale 2.0 (YFAS 2.0). The variables of interest were: (i) the proportion of patients meeting the diagnosis of food addiction (FA); (ii) FA symptoms count and severity; (iii) addictive-like eating profile severity. Their association with four main vascular risk factors (obesity, hypertension, dyslipidemia and diabetes) were tested using univariate and multivariate analyses. Results: Over a 4-month period, 101 patients (mean (standard deviation (SD)), 62.8 (13.7) years; males: 60.4%) were consecutively screened with the YFAS 2.0. Overall, 5% of the sample endorsed an FA diagnosis, and 38.6% screened positive for at least one of the symptom criteria, with the most frequently endorsed symptom being “Inability to cut down”. Dyslipidemia was the only vascular risk factor associated with the FA diagnosis (p = 0.043, 95% CI [−0.21; 0.01]). However, the severity of the addictive profile was associated with dyslipidemia (p = 0.016, 95% CI [−2.16; −0.21]) and diabetes (p = 0.038, 95% CI [−1.77; 0.25]), but only independently with dyslipidemia (p = 0.05; OR = 1.25; 95% CI [1.00; 1.56]). There were significant associations between the number of vascular risk factors and the severity of Time spent, Tolerance, and Use despite adverse consequences of FA symptoms, both in univariate and multivariate analyses (all p < 0.05). The number of vascular risk factors and total number of FA symptoms were significantly associated in univariate analyses (p = 0.007) but not after adjusting for age (p = 0.055) or sex (p = 0.083). Conclusions: This study highlighted the potential importance of addictive-like behaviours in the secondary prevention of stroke. However, larger and longer-term studies investigating addictive-like eating in diverse samples of stroke patients are warranted to achieve precision medicine. Full article