Search Results (10)

It is well known that carbonyl compounds play an important role in air pollution and the formation of secondary pollutants, such as peroxyacetyl nitrates (PAN). Additionally, airborne carbonyls have been described as cytotoxic, mutagenic and carcinogenic. In this research, several carbonyl compounds, including aldehydes and ketones, as well as ozone, were monitored during a campaign conducted in July and September-October 2023 at Golf Ciudad Real, a golf course located in a non-industrial area of a south-central province in Spain. Extraction and analysis were carried out following procedures outlined by Radiello^®. Analyses were performed using HPLC-DAD and UV-Visible spectrophotometry. Ozone shows seasonal variation (temperature-dependent) concentrations displaying lower values in September/October. Among all the identified carbonyls, butanal was the most abundant, accounting for 40% of the total concentration. The C₁/C₂ and C₂/C₃ ratios were also calculated to provide information about the main emissions sources of the analyzed carbonyl compounds, indicating that mainly anthropogenic sources contribute to air quality in the area. The data were further supported by Quantitative Structure-Activity Relationship (QSAR) models using the ProtoPRED online server, which employs in silico methods based on European Chemicals Agency (ECHA) regulations to assess the (eco)toxicity of the measured carbonyl compounds. Full article

The rapid progress of the modern world has resulted in new materials and products created at an accelerating pace. As such, nanoparticles have widespread applications and often find their way into the aquatic ecosystem. In the case of freshwater ecosystems, one of the commonly used bioindicators species used for pollution assessment is Daphnid magna. The Organization for Economic Co-operation and Development (OECD), and other organizations such as the European Chemicals Agency (ECHA) and Environmental Protection Agency (EPA), have set guidelines for acute toxicity testing in daphnids that are severely lacking in terms of information on the characteristics of the exposure vessel when studying the adverse effects of nanoparticles (NPs). Understanding the toxicity mechanisms of nanomaterials is imperative given the scarcity of information on their adverse effects. Furthermore, miniaturization of nanotoxicity assays can reduce the number of daphnids used, as well as the cost and nanomaterial waste, and provide results even at the individual animal level with enhanced reproducibility of testing. In this study, the impact of the exposure vessel on the observed physiological changes of daphnids was investigated for a silver nano ink. Exposures in eleven commercially available vessels; nine made of plastic and two made of glass were compared for 24 h. The effect of surface to volume ratio of the exposure vessel and the animal number or “crowding” during exposure was investigated in the context of miniaturizing biomarker assays as alternatives to traditional experimental setups in Daphnid magna. Toxicity curves showed differences depending on the vessel used, while a novel feeding rate assay and the activity of key enzymes were assessed as physiology endpoints. Full article

Building on our “Critical Inventory”, we analyse the need for the regulation of PVC plastics in the EU and its member states. To this end, we checked the three phases of the life cycle of PVC plastics: production, use and end-of-life. In the production phase, we focus on the economic relationships between PVC and chlor-alkali electrolysis, in particular, the dependence on the chlorine market and PVC sales. For the use phase, the health and environmental risks posed by many PVC additives are particularly relevant. The European Chemicals Agency (ECHA) has submitted well-founded proposals for the regulation of individual or defined groups of substances (e.g., ortho-phthalates), which we support. Problems that put a ban on the agenda stem in particular from the end-of-life phase of PVC plastics (PVC compounds), especially in the construction sector. Due to their long service life, a stock of around 160 million tonnes of PVC products in the EU has built up, increasingly finding its way into the waste management sector. Currently, there are no waste management infrastructures or facilities capable of disposing of these amounts. Without a phasing-out the production of virgin PVC (“PVC ban”), these quantities will continue to increase. We, therefore, come to the conclusion that post-consumer PVC should be collected separately and canalized into a disposal infrastructure designed for chlorine recovery. The European PVC industry “would welcome to make this separate collection mandatory”. Including the associated costs, PVC will probably lose its status as a particularly economically favourable plastic. Full article

In recent decades, nanotechnology has been rapidly advancing in various fields of human activity, including veterinary medicine. The review presents up-to-date information on recent advancements in nanotechnology in the field and an overview of the types of nanoparticles used in veterinary medicine and animal husbandry, their characteristics, and their areas of application. Currently, a wide range of nanomaterials has been implemented into veterinary practice, including pharmaceuticals, diagnostic devices, feed additives, and vaccines. The application of nanoformulations gave rise to innovative strategies in the treatment of animal diseases. For example, antibiotics delivered on nanoplatforms demonstrated higher efficacy and lower toxicity and dosage requirements when compared to conventional pharmaceuticals, providing a possibility to solve antibiotic resistance issues. Nanoparticle-based drugs showed promising results in the treatment of animal parasitoses and neoplastic diseases. However, the latter area is currently more developed in human medicine. Owing to the size compatibility, nanomaterials have been applied as gene delivery vectors in veterinary gene therapy. Veterinary medicine is at the forefront of the development of innovative nanovaccines inducing both humoral and cellular immune responses. The paper provides a brief overview of current topics in nanomaterial safety, potential risks associated with the use of nanomaterials, and relevant regulatory aspects. Full article

The proposal by the European Chemicals Agency (ECHA) to ban over 12,000 per- and polyfluoroalkyl substances (PFAS) has sparked a debate about potential consequences for the economy, industry, and the environment. Although some PFAS are known to be harmful, a blanket ban may lead to significant problems in attempting to replace PFAS-based materials for environmental transition, as well as in medical devices and everyday products. Alternative materials may potentially be less safe, as a rush to replace PFAS would reduce the time needed for toxicological analyses. Studies have shown that PFAS exhibit a diverse range of mechanisms of action, biopersistence, and bioaccumulation potential, and should thus not be treated as a single group. This is particularly true for the class of fluoropolymers. A targeted approach that considers the specific risks and benefits of each chemical may be more effective. Moreover, the proposed ban may also have unintended consequences for the environment as PFAS use is also associated with benefits such as reducing greenhouse-gas emissions and improving energy efficiency. Policymakers must carefully weigh up the potential consequences before making a final decision on the ban. Full article

This review focuses on the role of magnetic nanoparticles (MNPs), their physicochemical properties, their potential applications, and their association with the consequent toxicological effects in complex biologic systems. These MNPs have generated an accelerated development and research movement in the last two decades. They are solving a large portion of problems in several industries, including cosmetics, pharmaceuticals, diagnostics, water remediation, photoelectronics, and information storage, to name a few. As a result, more MNPs are put into contact with biological organisms, including humans, via interacting with their cellular structures. This situation will require a deeper understanding of these particles’ full impact in interacting with complex biological systems, and even though extensive studies have been carried out on different biological systems discussing toxicology aspects of MNP systems used in biomedical applications, they give mixed and inconclusive results. Chemical agencies, such as the Registration, Evaluation, Authorization, and Restriction of Chemical substances (REACH) legislation for registration, evaluation, and authorization of substances and materials from the European Chemical Agency (ECHA), have held meetings to discuss the issue. However, nanomaterials (NMs) are being categorized by composition alone, ignoring the physicochemical properties and possible risks that their size, stability, crystallinity, and morphology could bring to health. Although several initiatives are being discussed around the world for the correct management and disposal of these materials, thanks to the extensive work of researchers everywhere addressing the issue of related biological impacts and concerns, and a new nanoethics and nanosafety branch to help clarify and bring together information about the impact of nanoparticles, more questions than answers have arisen regarding the behavior of MNPs with a wide range of effects in the same tissue. The generation of a consolidative framework of these biological behaviors is necessary to allow future applications to be manageable. Full article

On 20 October 2020, the Working Group “Exposure Models” of the Europe Regional Chapter of the International Society of Exposure Science (ISES Europe) organised an online workshop to discuss the theoretical background of models for the assessment of occupational exposure to chemicals. In this report, participants of the workshop with an active role before and during the workshop summarise the most relevant discussion points and conclusions of this well-attended workshop. ISES Europe has identified exposure modelling as one priority area for the strategic development of exposure science in Europe in the coming years. This specific workshop aimed to discuss the main challenges in developing, validating, and using occupational-exposure models for regulatory purposes. The theoretical background, application domain, and limitations of different modelling approaches were presented and discussed, focusing on empirical “modifying-factor” or “mass-balance-based” approaches. During the discussions, these approaches were compared and analysed. Possibilities to address the discussed challenges could be a validation study involving alternative modelling approaches. The wider discussion touched upon the close relationship between modelling and monitoring and the need for better linkage of the methods and the need for common monitoring databases that include data on model parameters. Full article

In the last years, the evaluation and validation of exposure modelling tools for inhalation exposure assessment at workplaces received new and highly increased attention by different stakeholders. One important study in this regard is the ETEAM (Evaluation of Tier 1 Exposure Assessment Models) project that evaluated exposure assessment tools under the European REACH regulation (Registration, Evaluation, Authorisation and Restriction of Chemicals), (but next to the ETEAM project—as a project publicly funded by the German Federal Institute for Occupational Safety and Health (BAuA)—it is a rather new development that research groups from universities in Europe, but also internationally, investigated this issue. These other studies focused not only on REACH tier 1 tools but also investigated other tools and aspects of tool validity. This paper tries to summarise the major findings of studies that explored the different issues of tool validity by focusing on the scientific outcomes and the exposure on the science community. On the other hand, this publication aims to provide guidance on the choice and use of tools, addressing the needs of tool users. The consequences of different stakeholders under REACH are discussed from the results of the validation studies. The major stakeholders are: (1) REACH registrants or applicants for REACH authorisations, meaning those companies, consortia or associations who are subject to REACH; (2) Evaluating authorities within the scope of REACH, meaning the ECHA (European Chemicals Agency) secretariat and committees, but also the competent authorities of the member states or the European Union; (3) Developers of the different models and tools; (4) Users of the different models and tools. Full article

Engineered nanomaterials (ENMs) based on CeO₂ and TiO₂ differ in their effects on the unicellular green alga Raphidocelis subcapitata but these effects do not reflect the physicochemical parameters that characterize such materials in water and other test media. To determine whether interactions with algae can predict the ecotoxicity of ENMs, we studied the attachment of model compounds (three subtypes of CeO₂ and five subtypes of TiO₂) to algal cells by light microscopy and electron microscopy. We correlated our observations with EC₅₀ values determined in growth inhibition assays carried out according to the Organisation for Economic Co-operation and Development (OECD) test guideline 201. Light microscopy revealed distinct patterns of ENM attachment to algal cells according to the type of compound, with stronger interactions leading to greater toxicity. This was confirmed by electron microscopy, which allowed the quantitative assessment of particle attachment. Our results indicate that algal extracellular polymeric substances play an important role in the attachment of ENMs, influencing the formation of agglomerates. The attachment parameters in short-term tests predicted the toxicity of CeO₂ and TiO₂ ENMs and can be considered as a valuable tool for the identification of sets of similar nanoforms as requested by the European Chemicals Agency in the context of grouping and read-across. Full article

Coke production was classified as carcinogenic to humans by the International Agency for Research on Cancer. Besides polycyclic aromatic hydrocarbons, coke oven workers may be exposed to benzene and other volatile organic compounds (VOCs). The aim of this study was to assess the exposure to several VOCs in 49 coke oven workers and 49 individuals living in the same area by determining urinary mercapturic acids. Active tobacco smoking was an exclusion criterion for both groups. Mercapturic acids were investigated by a validated isotopic dilution LC-MS/MS method. Linear models were built to correct for different confounding variables. Urinary levels of N-acetyl-S-phenyl-L-cysteine (SPMA) (metabolite of benzene), N-acetyl-S-(2-hydroxy-1/2-phenylethyl)-L-cysteine (PHEMA) (metabolite of styrene), N-acetyl-S-(2-cyanoethyl)-L-cysteine (CEMA) (metabolite of acrylonitrile), N-acetyl-S-[1-(hydroxymethyl)-2-propen-1-yl)-L-cysteine and N-acetyl-S-(2-hydroxy-3-buten-1-yl)-L-cysteine (MHBMA) (metabolites of 1,3-butadiene) were 2–10 fold higher in workers than in controls (p < 0.05). For SPMA, in particular, median levels were 0.02 and 0.31 µg/g creatinine in workers and controls, respectively. Among workers, coke makers were more exposed to PHEMA and SPMA than foremen and engine operators. The comparison with biological limit values shows that the exposure of workers was within 20% of the limit values for all biomarkers, moreover three subjects exceeded the restrictive occupational limit value recently proposed by the European Chemicals Agency (ECHA) for SPMA. Full article