Search Results (16)

The enhancement of flavonoid content and antioxidant capacity in plants remains a significant area of focus in the investigation of plant-derived functional foods. This study systematically investigated the impact of exogenous zinc sulfate (5 mM ZnSO₄) stress on flavonoid content and antioxidant capacity in finger millet (Eleusine coracana L.) sprouts, along with its underlying molecular mechanisms. The results demonstrated that treatment with 5 mM ZnSO₄ significantly increased the flavonoid content in sprouts, reaching a maximum value of 5.59 μg/sprout on the 6th day of germination. ZnSO₄ stress significantly enhanced the activities of PAL, 4CL, and C4H, while also considerably upregulating the expression levels of flavonoid-biosynthesis-related genes. Physiological indicators revealed that ZnSO₄ stress increased the contents of malondialdehyde, hydrogen peroxide, and superoxide anion in the sprouts, while inhibiting sprout growth. As a stress response, ZnSO₄ stress enhances the antioxidant system by increasing antioxidant capacity (ABTS, DPPH, and FRAP), antioxidant enzyme activity (POD and SOD), and related gene expression (POD, CAT, and APX) in sprouts. This study provides experimental evidence for ZnSO₄ stress to improve flavonoid accumulation and antioxidant capacity in finger millet sprouts and provides important theoretical and practical guidance for the development of high-quality functional foods. Full article

Finger millet (Eleusine coracana L.) is a nutrient-dense cereal with high flavonoid content, yet the mechanisms regulating its secondary metabolite biosynthesis remain underexplored. Various exogenous stimuli can readily activate the enzymatic pathways and gene expression associated with flavonoid biosynthesis in plants, which are regulated by developmental cues. Research has established that methyl jasmonate (MeJA) application enhances secondary metabolite production in plant systems. This investigation examined MeJA’s influence on flavonoid accumulation and physiological responses in finger millet sprouts to elucidate the molecular mechanisms underlying MeJA-mediated flavonoid accumulation. The findings revealed that MeJA treatment significantly suppressed sprout elongation while enhancing the biosynthesis of total flavonoids and phenolic compounds. MeJA treatment triggered oxidative stress responses, with hydrogen peroxide and superoxide anion concentrations increasing 1.84-fold and 1.70-fold compared to control levels at 4 days post-germination. Furthermore, the antioxidant defense mechanisms in finger millet were upregulated following treatment, resulting in significant enhancement of catalase and peroxidase enzymatic activities and corresponding transcript abundance. MeJA application augmented the activities of key phenylpropanoid pathway enzymes—phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H)—and upregulated their respective gene expression. At 4 days post-germination, EcPAL and EcC4H transcript levels were elevated 3.67-fold and 2.61-fold, respectively, compared to untreated controls. MeJA treatment significantly induced the expression of downstream structural genes and transcriptional regulators. This study provides a deeper understanding of the mechanism of flavonoid accumulation in foxtail millet induced by MeJA, and lays a foundation for exogenous conditions to promote flavonoid biosynthesis in plants. Full article

Root endophytic microbial communities play a key role in plant health and productivity, yet the extent to which these communities vary across different crop species remains underexplored. This study aimed to investigate the root endophytic bacterial diversity of two important crops—hybrid tomato (Lycopersicon esculentum Mill. var. TNAU CO3) and finger millet (Eleusine coracana L. var. TNAU CO13)—to understand how crop-specific microbiomes contribute to agricultural sustainability and productivity. Targeted 16S rDNA amplicon sequencing was performed on tomato and finger millet root samples. A total of 165,772 and 181,327 16S rRNA gene sequences were generated from tomato and finger millet roots, respectively. These sequences were processed to identify amplicon sequence variants (ASVs), which were then classified taxonomically to assess community composition and functional prediction. Across the two hybrid crops, 1400 ASVs were detected in tomato and 1838 in finger millet. Proteobacteria (52.61–62.09%) were the dominant phylum in both, followed by Actinobacteria, Firmicutes, Bacteroidota, unidentified bacteria, Myxococcota, Verrucomicrobiota, Acidobacteriota, Fusobacteriota, and Chloroflexi. Finger millet roots harbored a more diverse and robust microbial assemblage, particularly enriched with nitrogen-fixing and oxidative stress-mitigating bacteria. In contrast, tomato roots showed a higher abundance of phosphate-solubilizing and biofilm-forming taxa, potentially enhancing resilience to environmental stress. These findings highlight the crop-specific nature of endophytic bacterial communities and their diverse functional capabilities. By revealing distinct microbial profiles in tomato and finger millet, this work provides a valuable foundation for developing strategies to optimize soil health, crop performance, and abiotic stress tolerance. Full article

Germination is an effective measure to regulate the accumulation of secondary metabolites in plants. In this study, we optimized the germination conditions of finger millet by response surface methodology. Meanwhile, physiological characteristics and gene expression were measured to investigate the mechanism of flavonoid accumulation in finger millet at the germination stage. The results showed that when germination time was 5.7 d, germination temperature was 31.2 °C, and light duration was 17.5 h, the flavonoid content of millet sprouts was the highest (7.0 μg/sprout). The activities and relative gene expression of key enzymes for flavonoid synthesis (phenylalanine ammonia-lyase, 4-coumarate-coenzyme a ligase, and cinnamate 4-hydroxylase) were significantly higher in finger millet sprouts germinated at 3 and 5 d compared with that in ungerminated seeds (p < 0.05). In addition, germination enhanced the activities of four antioxidant enzymes (catalase, peroxidase, superoxide dismutase, and ascorbate peroxidase) and up-regulated the gene expression of PAL and APX. Germination increased malondialdehyde content in sprouts, which resulted in cell damage. Subsequently, the antioxidant capacity of the sprouts was enhanced through the activation of antioxidant enzymes and the up-regulation of their gene expression, as well as the synthesis of active substances, including flavonoids, total phenolics, and anthocyanins. This process served to alleviate germination-induced cellular injury. These findings provide a research basis for the regulation of finger millet germination and the enhancement of its nutritional and functional properties. Full article

Finger millet (Eleusine coracana (L.) Gaertn.) is an annual allotetraploid that belongs to the grass family Poaceae subfamily Chloridoideae. Using less productive cultivars, biotic and abiotic stresses affect the yield and productivity of finger millet in Ethiopia. This research was aimed at investigating the acidity/Al tolerance of 328 finger millet accessions and 15 cultivars from Ethiopia and Zimbabwe. Prior to screening the accessions, optimization was performed on 15 cultivars and 15 accessions under three Al concentrations (0, 75, and 100 µM), and, afterward, 100 µM of Al concentration was selected as the threshold level. Root length (RL) and shoot length (SL) were recorded after 10 days of treatment. Accessions 215836, 215845, and 229722 and cultivars Urji, Bareda, and Axum were found Al-tolerant, while cultivars Tadesse, Padet, and Kumsa and accessions 212462, 215804, and 238323 were found Al-susceptible. ANOVA on RL indicated that the variance due to environment (42.3) was higher than genotypic variance (0.37). Whereas, the ANOVA on SL indicated the variance due to environment was not significant, and genotypic variance (0.18) was higher than environmental (0.02). RL was highly affected due to Al stress, while no distinct and visible symptoms were observed on SL. Furthermore, the screening of 328 accessions under 100 µM and the control resulted in Al-tolerant (n = 20), intermediate (225), and Al-susceptible (83). The results of the present study reveal that the presence of acid-tolerant accessions can be used as inputs for breeders to improve the productivity of finger millet in acidic areas. Full article

Finger millet (Eleusine coracana (L.) Gaertn.) is one of the most important cereal crops for people in Asia and Africa. It supplies energy and nutrients for all people, particularly poor people in these continents. Phosphorus (P) is an essential macronutrient for plant growth and grain quality, so an adequate amount of P is required for healthy plant life. There is considerable evidence that P deficiency affects the growths and yields of all cereals, including finger millet. The present study aimed at identifying the quantitative trait loci (QTL) for various agro-morphological and P-content traits under low P (LP) and high P (HP) conditions through linkage mapping. Two cultivars, IE-2606 (low-P-susceptible) and PR-202 (low-P-tolerant) were used to develop 100 recombinant inbred lines (RILs). In total, 55 QTL, associated with various agro-morphological (primary root length (PRL), shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW), root dry weight (RDW), and root hair density (RHD)) and P-content traits (inorganic phosphate content in shoots (PiS), inorganic phosphate content in roots (PiR), and total P content in shoots (TPS)) were detected under LP conditions. Similarly, 37 QTL for various agro-morphological (PRL, RFW, SDW, and root hair length (RHL)) and P-content traits (PiS, PiR, TPS, and total P content in roots (TPR)) were detected under HP conditions. Biotic and abiotic stress-responsive candidate genes linked to a few QTL were also identified. The identified QTL associated with agro-morphological and P-content traits in finger millet under P deficiency could be used in breeding programs to develop finger millet with better P use efficiency (PUE). Furthermore, improving finger millet growth and yield under LP soil will help to reduce Pi application and eutrophication, which may help to improve the lives of poor farmers and maintain sustainable environments in Asia and African countries. Full article

Increasing within-season dry spells in Southern Africa in recent years have generated growing interest in conservation agriculture (CA) to secure crop yields, especially under rainfed systems. This study aimed to evaluate the effects of CA on finger millet’s (Eleusine coracana (L.) Gaertn) growth, yield and water use efficiency on nutrient-depleted sandy soils. Five treatments, namely (conventional tillage (control), conventional tillage + mulch (partial CA1), reduced tillage only (partial CA2), reduced tillage + mulching (partial CA3) and reduced tillage + mulching + intercropping (full CA)) were evaluated over two consecutive cropping seasons (2015/16 and 2016/17) on-farm in the village of Chidora in Hwedza District, southeast Zimbabwe. All mulched treatments had 15–32% more soil water content over the two growing seasons compared to the control. The higher soil water content under the mulched treatments significantly improved finger millet growth and development during both seasons as evidenced by the lower number of days to emergence (3 days less), greater shoot biomass, higher number of productive tillers and higher number of fingers produced. The full CA treatment achieved the best finger millet grain yield of 1.07 and 1.29 t ha⁻¹ during the 2015/16 and 2016/17 seasons, respectively. Full CA, partial CA3 and partial CA1 increased finger millet grain yield by 70%, 14% and 17% during the 2015/16 cropping season compared to the control. During the 2016/17 cropping season, a similar trend in finger millet grain yield was observed. Full CA was also among the most efficient methods in terms of water utilization (WUE), especially during the 2015/16 season. We concluded that CA, particularly when practiced in full, was more effective at offsetting the water limitations imposed by intra-seasonal dry spells on finger millet and significantly improved productivity. Full article

Finger millet (Eleusine coracana (L.) Gaertn) is a highly nutritious crop, predominantly grown in the semi-arid tropics of the world. Finger millet has a niche market opportunity due to its human health benefits and being rich in calcium, iron and dietary fiber and gluten-free. Ethiopia is the center of the genetic diversity of the crop. However, the productivity of finger millet in the country is low (<2.4 tons ha⁻¹) compared with its potential yield (6 tons ha⁻¹). The yield gap in Ethiopia is due to a range of biotic and abiotic stresses and socio-economic constraints that are yet to be systemically documented and prioritized to guide future production and improved variety development and release. The objective of this study was to document finger millet production opportunities, constraints and farmer-preferred traits in Ethiopia as a guide to variety design in improvement programs. A participatory rural appraisal (PRA) study was undertaken in six selected districts of the Southern Nation Nationalities People Region (SNNPR) and Oromia Region in Ethiopia. Data were collected from 240 and 180 participant farmers through a semi-structured questionnaire and focus group discussion, respectively. Finger millet was the most important crop in the study areas grown mainly for a combination of uses, including for food, feed and cash (reported by 38.8% of respondent farmers), food and feed (14.5%), food and cash (13.7%), food (11.5%) and food, cash, feed and construction material (9.7%). Hand weeding was used by 59.2% of the respondent farmers, followed by hand weeding and chemical herbicides (40.8%). Finger millet was mainly planted as a sole crop (reported by 97.0% respondents), mixed (1.7%) and sole and mixed (1.3%). About 75.6% of respondent farmers only practiced finger millet rotation with other crops. Respondent farmers indicated their source of fresh seed was from the Bureau of Agriculture (49.1%), farmer-to-farmer seed exchange (22.1%), own saved seed (7.5%), local producers (7.5%), research institutions (5.8%), unknown sources (4.1%), local market (3.5%) and cooperatives (0.42%). The total cost of finger millet production per hectare was calculated at 1249 USD with a total income of 2139 USD/ha, making a benefit to cost ratio of 1.71:1.00 and indicating the relatively low yield gains using the currently grown varieties. The main constraints to finger millet production in the study areas were drought stress (reported by 41.3% respondents), lack of improved varieties (12.9%), lack of financial resources (11.3%), small land holdings (10.8%), limited access to seed (10.0%), a shortage of fertilizers (5.4%), poor soil fertility (4.6%), shortage of draught power (1.3%), labour shortages (1.3%) and high labour costs (1.3%). The most important farmer-preferred traits in a finger millet variety were high grain yield, compact head shape, ‘enjera’-making quality, high marketability and early maturity, resolved through principal component analysis. The above-mentioned production constraints and farmer-preferred traits are strategic drivers to enhance finger millet productivity and need to be incorporated into Ethiopia’s finger millet breeding and technology development. Full article

Finger millet is an important drought-tolerant and grain-nutrient dense food crop grown in semi-arid regions in Asia and Africa. The forage is used as a source of dry roughage for feeding livestock. In this study, the finger millet diversity panel (310 accessions and four controls) representing the global collection of the finger millet germplasm conserved at the ICRISAT genebank was assessed for forage quality and diversity in the years 2018 and 2019. Results of the study suggested that finger millet can generate stover yield ranging from 2890 to 10,779 kg ha⁻¹. Finger millet forage contained 6.47% to 8.15% of crude protein, >90% of dry matter content, 11.47% to 14.17% of ash content, 62.36% to 67.73% of neutral detergent fiber (NDF), 33.07% to 38.37% of acid detergent fiber (ADF), 3.95% to 4.80% of acid detergent lignin (ADL), 6.18% to 6.89% of metabolizable energy (ME) and 45.21% to 49.09% of in vitro organic matter digestibility (IVOMD) with the grain yield of 810 to 3698 kg ha⁻¹ at maturity stage. The per se performance between the races, regions, and biological status has been performed and differed significantly for important traits. The 314 accessions were grouped into five clusters based on the performance for food-feed traits. Except for crude protein, there was a positive relationship between forage quality-positive traits and grain yield, indicating that agronomic and forage quality traits could be improved simultaneously. The top 10 promising accessions for important forage quality traits and accessions with multiple forage quality traits were identified. This study provides a detailed understanding of the variability that exists in forage quality traits in crop residues and their association with agronomic traits in the finger millet germplasm. The identified top-performing accessions would be the key genetic resources for developing dual-purpose cultivars and the information from this study will be useful for future finger millet food-feed trait improvement. Full article

Improving iron (Fe) concentration in staple grain crops could help reduce Fe-deficiency anaemia in communities dependent on plant-based diets. Co-application of nitrogen (N) and zinc (Zn) fertilizers has been reported to improve both yield and grain Zn concentration of crops in smallholder farming systems. This study was conducted to determine if similar effects are observed for grain Fe concentration. Field experiments were conducted in two years, in two contrasting agro-ecologies in Zimbabwe, on maize (Zea mays L.), cowpea (Vigna unguiculata [L.] Walp) and two finger millet (Eleusine coracana (L.) Gaertn.) “seed pools”. The two finger millet “seed pools” were collected during previous farmer surveys to represent “high” and “low” Fe concentrations. All plots received foliar Fe-ethylene diamine tetra-acetic acid (EDTA) fertilizer and one of seven N treatments, representing mineral or organic N sources, and combinations thereof. Higher grain yields were observed in larger N treatments. Grain Fe concentration increased according to species: maize < finger millet < cowpea but varied widely according to treatment. Significant effects of N-form on grain Fe concentration were observed in the low finger millet “seed pool”, for which mineral N fertilizer application increased grain Fe concentration to a greater extent than other N forms, but not for the other species. Whilst good soil fertility management is essential for yield and grain quality, effects on grain Fe concentration are less consistent than reported previously for Zn. Full article

Fusarium is one of the most diverse fungal genera affecting several crops around the world. This study describes the phylogeny of Fusarium species associated with grains of sorghum and finger millet from different parts of Ethiopia. Forty-two sorghum and 34 finger millet grain samples were mycologically analysed. All of the sorghum and more than 40% of the finger millet grain samples were contaminated by the Fusarium species. The Fusarium load was higher in sorghum grains than that in finger millet grains. In addition, 67 test isolates were phylogenetically analysed using EF-1α and β-tubulin gene primers. Results revealed the presence of eight phylogenetic placements within the genus Fusarium, where 22 of the isolates showed a close phylogenetic relation to the F. incarnatum–equiseti species complex. Nevertheless, they possess a distinct shape of apical cells of macroconidia, justifying the presence of new species within the Fusarium genus. The new species was the most dominant, represented by 33% of the test isolates. The current work can be seen as an important addition to the knowledge of the biodiversity of fungal species that exists within the Fusarium genus. It also reports a previously unknown Fusarium species that needs to be investigated further for toxin production potential. Full article

Technological change has been the major driving force for increasing agricultural productivity and promoting agriculture development in developing countries. To improve the agricultural productivity and farmers’ livelihoods, several agricultural technologies (improved crop varieties and related agricultural practices) were introduced by various agencies to the farmers in the Rift Valley of Ethiopia. Thus, the objective of this study is to identify these technologies, and evaluate their characteristics and sustainability. The data were collected from farmers, agricultural extension workers, and agricultural experts, through a series of focus group discussions, key informant interviews, and farm observations, selected through purposive and random sampling techniques. Results showed that extension systems, social networks, or research projects were the agencies that introduced the technologies to the farmers. Haricot beans (Phaseolus vulgaris L.) and early and mid-maturing maize (Zea mays L.), as well as agricultural practices like row-sowing, banding fertilizer application, intercropping, and traditional rainwater-harvesting, were found to be in continuous use by the farmers. In contrast, the use of extra-early-maturing maize, sorghum (Sorghum bicolor L.) and finger millet (Eleusine coracana L.), as well as the use of related practices, including harvesting maize at physiological maturity, seed priming and fertilizer microdosing, were the technologies that were discontinued at the time of pursuing this study. Most of the continuing technologies had a high potential for reducing the vulnerability of the rain-fed agriculture to rainfall variability. Regardless of sources, the national extension system supported technologies that were integrated into the system only. Most of the discontinued technologies were found to be introduced by the research projects. These technologies were not brought into the attention of policy-makers for their integration into the extension system. The farmers also disliked a few of them for unfitting the existing socioeconomic setting. Whereas, the technologies that were introduced by the social networks were found to be widely used by the farmers, though they were not supported by the extension system. This is because most such technologies offer better yield and income. For instance, social networks have popularized haricot beans and hybrid maize because of their higher benefits to farmers. Farmers consider both socioeconomic and agroecological conditions for selecting and using technologies, whereas the extension system centers on existing agroecological conditions for recommending and supporting agricultural technologies. Consideration of both socioeconomic and agroecological settings would increase the prospect of a technology for sustainable adoption. Overall, rainfall variability, high price and poor access to improved seeds, farmers’ poor economic conditions, and the inadequate linkage between extension systems, social networks and research projects, remain critical factors influencing the sustainable use of agricultural technologies. It is, thus, commendable that policymakers should consider local socioeconomic and agroecological settings in recommending and supporting agricultural technologies besides instituting a strong consortium of extension systems, research institutes, research projects, social networks and farmers for improved agricultural technology development, extension system and sustainable adoption. Full article

Low forage quality of available perennial warm-season grasses during mid-summer through late summer affects the production of stocker cattle in the U.S. Southern Great Plains (SGP). Finger millet (Eleusine coracana Gaertn L.), which is a drought tolerant annual grass, could be a promising forage for the SGP. This field study assessed the adaptability and forage characteristics of 11 finger millet accessions originally sourced (1964–1981) from different parts of the world. Results of this study suggested that finger millet can generate forage yields ranging from 5.0 to 12.3 Mg ha⁻¹ 165 days after planting. Finger millet forage contained 105 to 156 g kg⁻¹ crude protein, 598 to 734 g kg⁻¹ neutral detergent fiber, 268 to 382 g kg⁻¹ acid detergent fiber, 597 to 730 g kg⁻¹ in vitro true digestibility, and 387 to 552 g kg⁻¹ neutral detergent fiber digestibility. Ten of the 11 accessions flowered and produced grains with yields varying from 60 to 1636 kg ha⁻¹. Overall, finger millet has the potential to serve as an alternative crop for the production of forage and possibly grain in the SGP. Further research needs to be focused on developing strategies for agronomic management and evaluating the capacity of finger millet under different grazing and hay production settings in the SGP. Full article

The present study examined the influence of malting on the phenolic composition of two cultivars of finger millet using an ultra-performance liquid chromatography mass spectrometer. Total polyphenols and antioxidant activities of the grains were also evaluated using sorghum as an external reference. Catechin, epicatechin, quercetin, taxifolin, and hesperitin were isolated flavonoids, whereas protocatechuic acid was the phenolic acid detected in finger millet malt. Increases in the content of catechin, epicatechin, and protocatechuic acid were observed for 72 h and 96 h for brown finger millet and sorghum malt. Complete loss of taxifolin and hesperitin were observed with the malting period for finger millet cultivars. A similar loss was noted in the proanthocyanidin A1/A2 and catechin content of sorghum with malting time. The grain malt exhibited 2,2-diphenyl-1-picrylhydrazyl,2,2′-azinobis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging and iron reducing activities. Increased ABTS and iron reducing activity with malting time were observed for the finger millet cultivars. The study demonstrates the presence of hesperitin in finger millet, and also shows that 72 h and 96 h of malting enhanced the catechin, epicatechin, and protocatechuic acid content, in addition to the antioxidant activity of the grain. Full article

This study evaluates the antioxidant potential and free radical scavenging ability of whole grain finger millet. The crude extracts were obtained by cold maceration using 80% ethanol and methanol. Concentrated extracts were subjected to radicals of 2,2’-diphenyl-1-picrylhydrazyl hydrate (DPPH), hydrogen peroxide, nitric oxide (NO) and malondialdehyde, while the antioxidant constituents evaluated includes total phenol and flavonoid. The results revealed that whole grain finger millet contained 14.65 ± 0.41 and 38.19 ± 0.60 mg QUE/g sample of flavoniods, 36.46 ± 1.09 and 67.10 ± 1.17 mg GAE/g sample of total phenolics and 17.61 ± 0.70 and 35.89 ± 1.32 mg AAE/g sample of total antioxidant capacity for methanol and ethanol respectively. Ethanolic extract of whole finger millet scavenged above 50% hyrogen peroxide, NO and DPPH radicals. Hence, it terminated up to 8.97–65.16% lipid peroxidation reaction chain that may result from auto-oxidation, photo-oxidation and any other oxidation stress that may occur in plant and animal tissues. Therefore, pharmocology attention has to be given to finger millet which showed an effective antioxdants and antiradical capacities. Also, cultivation of finger millet should be encouraged by farmers. Full article