Search Results (54)

Circulating tumor cell enrichment and enumeration are advancing early detection of cancer, monitoring of therapy response, and even next-generation therapies. Efficiently capturing rare cells from complex biological fluids is essential in both diagnostic and therapeutic applications. EpCAM-positive tumor cells are specifically captured by utilizing covalently immobilized anti-EpCAM monoclonal antibodies onto the surface of chemically modified glass microbeads. To maximize the capture efficiency, bead geometry, immobilization conditions, flow rate, and anticoagulant dosage were systematically optimized. An in vitro flow-capture system was designed and used to evaluate the capture efficiency of the proposed technology by utilizing HTC116 colon cancer cell-spiked model media. The effect of substrate surface pretreatment was characterized by goniometry, while the capture performance was monitored by flow cytometry and fluorescent microscopy. The specific capture ability of the bioactive microbead substrate reached over 130,000 cells in the laboratory-scale cartridge (V(cartridge) = 2.6 cm³; m(bead) = 4 g). This capture efficiency suggests a promising rare-cell capture utilization of the proposed technology and may be used for research, diagnostic, and therapeutic purposes. In this paper, we reported on the development and feasibility test of a high-performance bioactive glass-microbead cell capture substrate. Due to the relevance and novelty of the reported results, with further development, the versatile platform technology presented could be readily implemented to capture tumor cells from complex biological samples and represent an additional complementary tool to existing cancer diagnostics and therapies. Full article

The quantity of construction demolition waste (CDW) has been increasing due to the demolition of many old buildings throughout the world. So far, all the statistics indicate that there is a very large generation of CDW, which increases annually. The increasing amount CDW in landfills will cause a scarcity of landfill space and will also increase pollution and cost due to transportation. Recycled brick aggregate concrete (RBAC) incorporating polystyrene (EPS) aggregate beads has emerged as an alternative lightweight material with numerous obvious sustainable benefits, suitable for a future circular economy. The goal of this paper is to assess the feasibility of obtaining lightweight aggregate concrete of structural grade with recycled brick aggregate (RBA) as a coarse aggregate and the incorporation of polystyrene beads in a certain percentage by conducting an experimental study on the dry and apparent density, compressive strength, split-tensile strength and elasticity modulus. In addition, the effects of the w/c ratio and cement content on these properties were studied to provide useful information for the performance optimization of this concrete with RBA and polystyrene (EPS) beads. The properties were investigated for two cement contents, 400 and 360 kg/m³, and two ratios between water and cement, 0.43 and 0.39, respectively. The RBAC mixtures containing EPS beads in 15%, 25% and 35% replacement percentages were evaluated through a comprehensive test program based on the European standards. The results showed that, in general, the use of polystyrene (EPS) beads decreased the mechanical properties of the recycled brick aggregate concrete; however, the outcome indicates the potential for producing lightweight concrete of different grades, including structural classes. It was found that the developed lightweight concrete presents a uniform distribution of the polystyrene granules in the hardened volume of concrete. Also, it was found that the recycled brick aggregate with a 16 mm maximum size did not negatively influence the uniform distribution of the EPS beads, avoiding concentrations of beads. With the increase in the percentage of EPS beads, the properties of the recycled brick aggregate concrete were found to be less sensitive to the water-to-cement ratio. Full article

The characteristics of a variable polarity plasma arc (VPPA) and the keyhole behavior significantly influence weld formation. This study investigates the impact mechanism of welding current on weld formation by examining both arc thermal-force output and keyhole behavior through a combination of numerical analysis and experimental methods. A three-dimensional transient arc model with alternating loading of electrode negative (EN) and electrode positive (EP) polarity arcs is developed based on magnetohydrodynamics and is enhanced by user-defined scalars (UDS). The analysis of the arc characteristics reveals that the arc in the EN phase exhibits a larger arc penetration force and keyhole digging effect, while a divergence of the arc occurs in the EP phase. The thermal force of the arc exhibits periodic variation with changes in arc polarity. EN and EP arcs associated with “critical current difference” have minimal thermal fluctuations, minimal fluctuations in the keyhole dimensions (the keyhole long-axis size and keyhole area fluctuation ranges are 4.5–5.2 mm and 78–83 mm², respectively), and the best keyhole stability and weld bead formation. Otherwise, the fluctuation of the keyhole long-axis size and keyhole area can be very large, which may lead to an unstable keyhole molten pool and poor weld formation. Full article

Background/Objectives: The PowerMag system (PM) is a platform for the isolation of circulating tumor cells (CTCs) by the depletion of CD45⁺-leukocytes. However, an EpCAM⁻CD45⁻ cell population is present in large numbers in the cell filtrates collected by PM. This lowers the purity of the CTCs and negatively impacts their molecular characterization. The aims of this study are to characterize the cellular properties of the EpCAM⁻CD45⁻ cells and to upgrade the system to improve CTC purity. Methods: A real-time RT-PCR assay, Liu’s stain analysis, and Annexin V (AnxV) binding assay were used to define the cellular properties of the EpCAM⁻CD45⁻ cells. An upgraded system was developed to remove the EpCAM⁻CD45⁻ cells and improve the CTC purity. Clinical blood samples were used to evaluate the performance of the system. Results: The EpCAM⁻CD45⁻ cells were defined as apoptotic cells, which displayed apoptotic body-like morphology and elicited AnxV binding activity. AnxV beads developed in-house can effectively bind and remove EpCAM⁻CD45⁻ cells from the cell filtrates. An improved generation of a CTCs isolation platform, designated as PM II, was developed by integration of AnxV beads into the workflow to remove the apoptotic cells. PM II recovered CTCs with improved CTC purity by effective removal of the background apoptotic cells. The improved performance of PM II allowed for direct profiling of cancer-related gene mutations by next-generation sequencing without cell picking and further purification. Conclusions: PM II holds great promise as a platform for isolating CTCs with improved purity and for exploring its application in cancer diagnosis and monitoring in a clinical setting. Full article

Expanded polystyrene (EPS) bead–lightweight soil composites are a new type of artificial geotechnical material with low density and high strength. We applied EPS bead–lightweight soil in this project, replacing partial cement with fly ash to reduce construction costs. EPS beads were used as a lightweight material and cement and fly ash as curing agents in the raw soil were used to make EPS lightweight soil mixed with fly ash. The EPS bead proportions were 0.5%, 1%, 1.5%, and 2%; the total curing agent contents were 10%, 15%, 20%, and 25%; and the proportions of fly ash replacing cement were 0%, 15%, 30%, 45%, and 60%, respectively. Unconfined compressive strength (UCS) and scanning electron microscopy (SEM) tests were conducted. The results showed that the EPS content, total curing agent content, and proportion of fly ash replacing cement had a significant impact on the UCS of the lightweight soil. This decreased with an increase in EPS content and decrease in total curing agent content and decreased with increased proportions of fly ash replacing cement. When the proportion of fly ash replacing cement was not too high, the strength of the lightweight soil decreased less, and its performance still met engineering needs. At the same time, the soil can also consume fly ash and reduce environmental pollution. EPS lightweight soil mixed with fly ash still has advantages, and it is recommended to keep the proportion of fly ash replacing cement less than 30%. The failure patterns for lightweight soil mainly include splitting failure, oblique shear failure, and bulging failure, which are related to the material mix ratio. Full article

Stress-inducible heat shock protein 70 (Hsp70), which functions as a molecular chaperone and is frequently overexpressed in different cancer cell types, is present on the cell surface of tumor cells and is actively released into the circulation in free and extracellular lipid vesicle-associated forms. Since the exact pathomechanism of endometriosis has not yet been elucidated (although it has been associated with the development of endometrial and ovarian cancer), we asked whether extracellular Hsp70 and circulating endometriotic cells (CECs) reflect the presence and development of endometriosis. Therefore, circulating levels of free and lipid microvesicle-associated Hsp70 were measured using the Hsp70-exo ELISA, and the presence of circulating CECs in the peripheral blood of patients with endometriosis was determined using membrane Hsp70 (mHsp70) and EpCAM monoclonal antibody (mAb)-based bead isolation approaches. Isolated CECs were further characterized by immunofluorescence using reagents directed against cytokeratin (epithelial marker), CD45 (leukocyte marker), CD105/CD44 (mesenchymal stemness markers) and by comparative RNA analysis. Similar to the situation in patients with cancer, the levels of circulating Hsp70 were elevated in the blood of patients with histologically proven endometriosis compared to a healthy control cohort, with significantly elevated Hsp70 levels in endometriosis patients with lesions outside the uterine cavity. Moreover, CECs could be isolated using the cmHsp70.1 mAb-based, and to a lesser extent EpCAM mAb-based, bead approach in all patients with endometriosis, with the highest counts obtained using the mHsp70-targeting procedure in patients with extra-uterine involvement. The longevity in cell culture and the expression of the cytokeratins CD105 and CD44, together with differentially expressed genes related to epithelial-to-mesenchymal transition (EMT), revealed similarities between mHsp70-expressing CECs and circulating tumor cells (CTCs) and suggest a mesenchymal stem cell origin. These findings support the involvement of mHsp70-positive stem cell-like cells in the development of endometriotic lesions. In summary, elevated levels of Hsp70 and CECs in the circulation could serve as liquid biopsy markers for endometriosis with extra-uterine involvement and help to elucidate the underlying pathomechanism of the disease. Full article

This study investigates a sustainable coating method for modified expanded polystyrene (MEPS) beads to improve the thermal insulation of lightweight concrete intended for wall application. The method employed in this study is based on a novel coating technique that represents a significant advancement in modifying Expanded Polystyrene (EPS) beads for enhanced lightweight concrete. This study experimentally assessed the energy-saving capabilities of MEPS concrete in comparison to control groups of uncoated EPS beads and normal concrete by analysing early-stage temperature, thermal conductivity, specific heat capacity, heat flux, and thermal diffusivity. The thermal conductivity of MEPS concrete is approximately 40% lower than that of normal concrete, demonstrating its usefulness in enhancing insulation. The heat flux calculated for MEPS concrete is significantly reduced (approximately 35%), and it has a 20% lower specific heat capacity than ordinary concrete, indicating a reduction in energy transfer through the material and, thus, potential energy-efficiency benefits. Furthermore, the study discovered that all test objects have very low thermal diffusivity values (less than 0.5 × 10⁻⁶ m²/s), indicating a slower heat transport through the material. The sustainable coating method utilized fly ash-enhanced thermal efficiency and employed recycled materials, hence decreasing the environmental impact. MEPS concrete provides a practical option for creating sustainable and comfortable buildings through the promotion of energy-efficient wall construction. Concrete incorporating coated EPS can be a viable option for constructing walls where there is a need to balance structural integrity and adequate insulation. Full article

The heterogeneity of circulating tumor cells has a significant impact on the diagnosis, treatment, and monitoring of cancer. Research on the subtypes of circulating tumor cells can bring better treatment outcomes for cancer patients. Here, we proposed a microfluidic chip for the magnetic capture of subtypes of circulating tumor cells from the whole blood and phenotypic profiling by stacking laminar flow vertically. Circulating tumor cells were sorted and captured by the three-dimensional regulation of both magnetic fields in the vertical direction and flow fields in the lateral direction. Using EpCAM-magnetic beads, we achieved sorting and sectional capture of target cells in whole blood and analyzed the surface expression levels of the captured cells, confirming the functionality of the microfluidic chip in sorting and capturing subtypes of circulating tumor cells. This microfluidic chip can also aid in the subsequent subtype analysis of other rare cells. Full article

In this study, polyurethane-polystyrene composites (RPURF-EPS) were obtained with the co-expansion method. This method consists of utilizing the heat of the exothermic reaction of polyurethane (PUR) formation to expand polystyrene beads (PSBs). The materials were obtained using polyurethane systems based on the selected blowing agents, such as cyclopentane, a mixture of fluorocarbons and water. The analysis of the foaming process was carried out using a special device called FOAMAT. The characteristic start, rise, gelation and curing times were defined. The rise profile, the reaction temperature, the pressure and the dielectric polarization were measured. The influence of selected blowing agents on the cell structure and physical–mechanical properties of reference rigid polyurethane foam (RPURF) and RPURF-EPS, such as apparent density, compressive strength and thermal conductivity, were evaluated. Based on the research, the blowing agents that have the most beneficial influence on the properties and structure of the composites and that provide the most efficient expansion of PSBs in a light porous composite were found. Full article

Plastics represent an integral part of our everyday lives, with various functions from packaging materials to insulation layers in our buildings. Pure expanded polystyrene (EPS) is a good example of a fully recyclable material. However, once polluted with other materials or substances, EPS becomes a serious environmental burden. In this work, waste EPS for the production of greener building composites with balanced properties and utility value was investigated. Natural aggregate (2/4 mm) was substituted with corresponding fractions of a thermoplastic alternative in portions of 25, 50, 75, and 100 vol.%. The comprehensive experimental investigation evaluated physical and mechanical properties, heat transport and accumulation, and water absorption characteristics. Due to the uniformly distributed plastic particles in the hardened cement-based matrix, the data revealed an important reduction in the dead weight of produced mortars, which also reduced thermal conductivity by up to 47%. On one hand, lightweight mortars showed partially reduced mechanical resistivity; on the other hand, the EPS bead-modified structure turned out to be effective in liquid water transport reduction. Full article

The expansion of China’s highways and railways, as well as the growing demand for them, has focused attention on the impact of traffic loads on foundation settling, uneven deformation, and ground cracking. These effects have garnered considerable research attention, with particular emphasis placed on integrating innovative materials into the soil matrix. This investigation involved loading experiments utilizing a combination of lightweight soil, expanded polystyrene (EPS), and cement. Consolidation tests assessed the extent of deformation and settlement, incorporating varying proportions of EPS and cement. The test results show that when subjected to confined conditions, the stress–strain relationship curve assumes a hyperbolic shape closely linked to the e-p curve. This shape effectively captures the unique structural characteristics exhibited by lightweight soils. As the size of the EPS particles and the applied stress increase, a corresponding rise in the strain of the specimens is observed. Simultaneously, as the strain magnitude increases, the elastic modulus experiences a decline. Additionally, it is noted that this trend further increases as the doping of the cement with EPS particles increases. When the EPS volume ratio and cement mix ratio remain constant across different specimens, there is a decrease in structural strength as the size of the EPS increases. In lightweight soil, settlement can occur rapidly, with approximately 95% of total consolidation deformation happening within a few minutes, which suggests that the settlement is instantaneous and primarily consolidation settlement. The structural strength of lightweight soil shows a negative correlation with the size of EPS, implying that larger EPS size may lead to a reduction in strength. Therefore, it is recommended to consistently use EPS beads with a diameter of 3–4 mm during construction. Full article

Additive manufacturing by 3D printing has emerged as a promising construction method offering numerous advantages, including reduced material usage and construction waste, faster build times, and optimized architectural forms. One area where 3D printing’s potential remains largely unexplored is in combination with lightweight materials, especially lightweight gypsum. This research paper explores the potential of combining 3D printing technology with lightweight gypsum-based composites to extend the relatively limited gypsum application possibilities in the construction industry. The study investigates the use of expanded polystyrene (EPS) beads as an aggregate in gypsum composites, focusing on the printability of the mixture and hardened state mechanical properties in various print directions. Mechanical tests reveal that 3D printing can reduce the compressive strength of the EPS–gypsum composite by between 3% and 32%, and the flexural strength by up to 22%, depending on testing direction. However, the technology opens up new production possibilities for applications where such strength can be sufficient. The study describes that a slight increase in the water-to-gypsum (W/G) ratio in 3D-printed mortars enhances homogeneity and reduces porosity, resulting in improved structural uniformity and therefore higher flexural and compressive strength values. Furthermore, the paper discusses the mechanical anisotropy observed in 3D-printed samples. The combination of 3D printing technology and lightweight gypsum offers the potential for sustainable construction practices by reusing waste materials and creating lightweight, thermally and acoustically insulative, as well as architecturally diverse building components. Full article

The innovation inherent to employing expanded polystyrene (EPS) beads lies in its transformative impact on traditional concrete practices. Through the incorporation of EPS beads in concrete mixtures, a novel approach emerges that significantly alters the material’s characteristics, and opens up new avenues for construction and design. Studying the shear behavior of RC beams made with EPS beads is essential for advancing knowledge, improving design practices, ensuring structural integrity, and promoting the effective and responsible use of innovative materials in construction. This research experimentally investigated the effect of using EPS beads and pozzolana aggregate (PA) on the shear behavior of the RC beams. A total of 27 simply supported rectangular beams were cast, using three novel distinct mix designs, and were subjected to two-point load testing until failure. These three mixes were categorized as follows: a control mix, a mix with only EPS, and a mix with EPS, along with an additive. The ultimate failure load was experimentally recorded for all specimens, and the influence of the temperature (300 °C and 600 °C) on the RC beams made with EPS was examined. The findings revealed a reduction in the concrete compressive strength and density in the beams containing EPS and EPS with superplasticizers of (21.7%, 24.9%) and (11.3%, 16.2%), respectively. Additionally, EPS played a significant role in diminishing the ultimate shear capacity of the beams, compared to the control beams, by about 19.4%. However, the addition of a superplasticizer along with the EPS helped to maintain the beam capacity, to some extent. Conversely, the beams exposed to a temperature of 300 °C exhibited an almost similar capacity to that of the control beams without heating. Nevertheless, at 600 °C, the beams displayed a noticeable decrease in the ultimate load capacity, compared to the unheated control beams. Full article

Limited investigations have evaluated the effect of expanded polystyrene (EPS) beads on the structural lightweight concrete properties. EPS offers many features compared to natural or artificial lightweight aggregates including the elimination of aggregate saturation prior to concrete batching, ability to be fabricated on site, consistency in size and quality, and reduced cost. The main objective of this paper is to assess the suitability of finite element (FE) modeling based on deterministic and stochastic approaches to predict the shear strength behavior of reinforced concrete (RC) beams containing EPS additions. Test results showed that the experimental load-deflection properties recorded at failure can be well reproduced using both FE approaches. Nevertheless, the damaged-zone distribution and crack patterns that occur during the loading stages of RC beams cannot be approximated using the deterministic FE approach. In contrast, the stochastic method was quite suitable as it accounted for the concrete heterogeneity and altered spatial mechanical properties (such as compressive strength, splitting tensile strength, and Young’s modulus) due to EPS additions. Such data can be of interest to civil engineers seeking to predict the failure patterns and performance of structural lightweight members while reducing the time and resources needed to account for the concrete’s strength variability during experimental testing. Full article

Heat shock protein 70 (Hsp70) is frequently overexpressed in many different tumor types. However, Hsp70 has also been shown to be selectively presented on the plasma membrane of tumor cells, but not normal cells, and this membrane form of Hsp70 (mHsp70) could be considered a universal tumor biomarker. Since viable, mHsp70-positive tumor cells actively release Hsp70 in lipid micro-vesicles, we investigated the utility of Hsp70 in circulation as a universal tumor biomarker and its potential as an early predictive marker of therapeutic failure. We have also evaluated mHsp70 as a target for the isolation and enumeration of circulating tumor cells (CTCs) in patients with different tumor entities. Circulating vesicular Hsp70 levels were measured in the peripheral blood of tumor patients with the compHsp70 ELISA. CTCs were isolated using cmHsp70.1 and EpCAM monoclonal antibody (mAb)-based bead approaches and characterized by immunohistochemistry using cytokeratin and CD45-specific antibodies. In two out of 35 patients exhibiting therapeutic failure two years after initial diagnosis of non-metastatic breast cancer, progressively increasing levels of circulating Hsp70 had already been observed during therapy, whereas levels in patients without subsequent recurrence remained unaltered. With regards to CTC isolation from patients with different tumors, an Hsp70 mAb-based selection system appears superior to an EpCAM mAb-based approach. Extracellular and mHsp70 can therefore serve as a predictive biomarker for therapeutic failure in early-stage tumors and as a target for the isolation of CTCs in various tumor diseases. Full article