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27 pages, 15147 KB  
Article
Aerodynamic Performance of Steam Turbine Blades with Influence of Tip Seal Leakage Flow
by Lihua Cao, Dacai Li, Lei Wang, Heyong Si and Zhongbin Zhang
Processes 2026, 14(17), 2728; https://doi.org/10.3390/pr14172728 - 26 Aug 2026
Viewed by 335
Abstract
Tip seal leakage in shrouded steam turbines can significantly affect the aerodynamic performance of downstream blade rows. An unsteady three-dimensional numerical model of a 1.5-stage high-pressure steam turbine is established using ANSYS CFX (ANSYS2021) with the SST k–ω turbulence model. Tip seal clearances [...] Read more.
Tip seal leakage in shrouded steam turbines can significantly affect the aerodynamic performance of downstream blade rows. An unsteady three-dimensional numerical model of a 1.5-stage high-pressure steam turbine is established using ANSYS CFX (ANSYS2021) with the SST k–ω turbulence model. Tip seal clearances of 1.0, 1.5, and 1.9 mm are investigated using the Q-criterion and vorticity transport equation to characterize leakage-vortex evolution and its interaction with the mainstream. The results show that increasing tip seal clearance strengthens leakage flow and expands its interaction region. The expansion term exhibits a relatively stronger influence on vorticity variation near the seal teeth, whereas the vortex stretching term plays a significant role in leakage-vortex evolution near the seal inlet, outlet, and cavity. Leakage vortices interact with the rotor wake, intensifying velocity gradients and aerodynamic loss, with pronounced flow distortion near 85% rotor span. The disturbance is further transported to the downstream stator, causing marked variations in flow angle, circumferential velocity, and static pressure in the upper-span region. At 95% and 99% blade heights, pronounced differences in suction-surface static pressure occur within the forward 80% of the chord length. These findings clarify the aerodynamic consequences of tip seal leakage and its downstream effects. Full article
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23 pages, 11310 KB  
Article
Performance Enhancement of the Passive Heat Exchanger in the MNTZV-159 Metal Hydride Storage System Using Triply Periodic Minimal Surface (TPMS) Structures
by Šimon Hudák, Marián Lázár, Gabriela Ižaríková, Tomáš Brestovič, Natália Jasminská, Peter Čurma, Romana Dobáková and Peter Milenovský
Materials 2026, 19(16), 3539; https://doi.org/10.3390/ma19163539 - 20 Aug 2026
Viewed by 286
Abstract
Low thermal conductivity of metal hydride beds significantly limits the hydrogen absorption kinetics and performance of metal hydride storage systems. This study presents a new design of a passive internal heat exchanger for a certified MNTZV-159 low-pressure hydrogen storage tank using the Triply [...] Read more.
Low thermal conductivity of metal hydride beds significantly limits the hydrogen absorption kinetics and performance of metal hydride storage systems. This study presents a new design of a passive internal heat exchanger for a certified MNTZV-159 low-pressure hydrogen storage tank using the Triply Periodic Minimal Surface (TPMS) structures. The parametric improvement in the design of a cylindrical Diamond TPMS-based geometry was performed by applying various cell dimensions, arc counts, and wall thicknesses while maintaining the original volume of the heat exchanger. The analysed configuration was subsequently evaluated through three-dimensional numerical heat-transfer simulations conducted in ANSYS CFX. Compared with the original finned heat exchanger, the TPMS-based design reduced the average metal hydride temperature from 107.7 °C to 86.2 °C and the maximum temperature from 130.9 °C to 114.0 °C. The improved temperature uniformity enhanced the heat removal from the hydride bed and created more favourable conditions for hydrogen absorption. The results demonstrated that TPMS structures constitute a promising solution for improving passive thermal management in metal hydride hydrogen storage systems while maintaining the storage capacity of the vessel. Full article
(This article belongs to the Special Issue Hydrides for Energy Storage: Materials, Technologies and Applications)
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29 pages, 7081 KB  
Article
Application of an Off-Design Transient Simulation Framework for Pump-as-Turbine in OpenFOAM: Validation and Flow Analysis
by Tomas Valldeperas, Raúl Martínez-Cuenca, Diego Benedetti, Jacopo C. Alberizzi and Massimiliano Renzi
Energies 2026, 19(16), 3777; https://doi.org/10.3390/en19163777 - 11 Aug 2026
Viewed by 264
Abstract
Pump-as-Turbine (PaT) systems represent a cost-effective solution for hydraulic energy recovery in existing water networks and industrial processes. However, the prediction of their performance in turbine mode remains challenging, especially under off-design conditions where unsteady flow structures and internal losses strongly affect the [...] Read more.
Pump-as-Turbine (PaT) systems represent a cost-effective solution for hydraulic energy recovery in existing water networks and industrial processes. However, the prediction of their performance in turbine mode remains challenging, especially under off-design conditions where unsteady flow structures and internal losses strongly affect the machine efficiency. In this work, transient CFD simulations of a real industrial centrifugal pump operating as a turbine are performed using OpenFOAM and ANSYS CFX and compared with available experimental data. The investigated operating range extends from 0.7QBEP to 1.3QBEP. A mesh independence analysis is first carried out using the Grid Convergence Index method, leading to the selection of a mid-size computational mesh as a compromise between accuracy and computational cost. The transient OpenFOAM results show close agreement with the ANSYS CFX predictions over the complete operating range. Both numerical frameworks reproduce the experimental hydraulic-efficiency trend and the location of the BEP, while systematic deviations in hydraulic head and mechanical power are mainly attributed to the geometrical and physical simplifications adopted in the common computational model. The local pressure coefficient monitored at the tongue region shows that both the mean pressure level and the fluctuation amplitude increase with flow rate, indicating stronger transient behavior under high-flow conditions. Beyond the global performance comparison, the flow field is analyzed using Qcrit iso-surfaces, mean circumferential velocity, the swirl-intensity parameter Sint, relative velocity fields at the PaT operational leading edge, and volute head-loss evaluation. The results show that part-load operation is characterized by strong outlet vortical structures and high residual swirl intensity, while the BEP region corresponds to reduced outlet rotational content. Under overload conditions, the outlet swirl remains limited, but the volute head loss increases significantly, becoming a dominant contributor to the efficiency drop. The study demonstrates that PaT performance cannot be interpreted from outlet swirl alone, but results from the combined effect of residual rotational structures, tongue-region unsteadiness, impeller incidence conditions, and volute dissipation. Full article
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27 pages, 3614 KB  
Article
Comprehensive Design and Structural Verification of a Tubular Steel Metal–Hydride Storage Vessel for Hydrogen Separation and Storage
by Lukáš Tóth, Filip Duda, Ivan Mihálik, Viktória Rajťúková and Anton Hovana
Energies 2026, 19(16), 3768; https://doi.org/10.3390/en19163768 - 11 Aug 2026
Viewed by 354
Abstract
Hydrogen storage and separation remain major technical challenges limiting the broader implementation of hydrogen-based energy systems. Metal–hydride alloys offer a promising solution because they enable reversible hydrogen storage within their crystal structure and can selectively absorb hydrogen from multicomponent gas mixtures. However, the [...] Read more.
Hydrogen storage and separation remain major technical challenges limiting the broader implementation of hydrogen-based energy systems. Metal–hydride alloys offer a promising solution because they enable reversible hydrogen storage within their crystal structure and can selectively absorb hydrogen from multicomponent gas mixtures. However, the practical application of metal–hydride systems requires storage vessels that combine sufficient mechanical strength with effective heat removal, as hydrogen absorption is accompanied by significant heat generation that can reduce the reaction rate and usable storage capacity. This study addresses hydrogen storage within the crystal structure of metal alloys and introduces the potential of metal–hydride (MH) alloys for hydrogen separation from gas mixtures. It subsequently presents the structural design and strength assessment of a low-pressure, double-walled, tubular steel MH storage vessel intended for hydrogen storage in a MnTiVFeZr-based alloy. Structural simulations were performed in ANSYS 2025 R2 Static Structural at three operating pressures: 3, 5, and 7 MPa. For all three simulated pressure conditions, the gravimetric hydrogen storage capacity of the alloy was 0.992 ± 0.016 wt.%. Following the selection of the most suitable design with an operating pressure of 3 MPa, an analytical calculation was performed to verify the results obtained from the numerical analysis. The storage vessel was subsequently manufactured and subjected to experimental strength validation using the test procedures specified in the STN EN 13322-2 standard. The design of the low-pressure tubular steel MH storage vessel also incorporates an efficient thermal management system based on a combination of active and passive cooling modules. The passive cooling module takes the form of an internal heat-transfer enhancement element, which is inserted into the primary storage vessel together with the MH alloy. The active cooling module uses a coolant flowing around the outer wall of the primary vessel. The optimal design of the aluminium passive cooling module was selected from four variants based on a steady-state temperature-field analysis conducted in ANSYS CFX. The selected module was subsequently manufactured and integrated into the proposed storage vessel. The vessel equipped with the passive cooling element was then subjected to experimental temperature measurements during hydrogen absorption by the MH alloy. The experimentally obtained data were compared with the numerical simulation results to evaluate the temperature fields within the vessel and the heat dissipation from the core of the MH storage system during hydrogen absorption. The main contribution of this work is the development of a mechanically validated and thermally managed tubular metal–hydride vessel that integrates structural design, numerical optimisation, manufacturing, and full-scale experimental testing within a single methodology. The proposed approach provides a practical basis for the further development and scaling of low-pressure metal–hydride systems for hydrogen storage, purification, and separation applications. Full article
(This article belongs to the Section A5: Hydrogen Energy)
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36 pages, 18078 KB  
Article
CFD Investigation of Cavitation Effects on High-Speed Propeller Performance
by Adrian Popa, Alecu Toma, Octavian-Narcis Volintiru, Daniel Mărășescu, Doru Coșofreț, Florențiu Deliu and Ciprian Popa
Appl. Sci. 2026, 16(15), 7843; https://doi.org/10.3390/app16157843 - 6 Aug 2026
Viewed by 305
Abstract
Cavitation represents a critical phenomenon affecting the performance, durability, and acoustic signature of high-speed propellers. This study extends a previous non-cavitating analysis of the same propeller by investigating cavitation onset, evolution and performance change for a 140 mm diameter, three-blade fixed-pitch high-speed propeller. [...] Read more.
Cavitation represents a critical phenomenon affecting the performance, durability, and acoustic signature of high-speed propellers. This study extends a previous non-cavitating analysis of the same propeller by investigating cavitation onset, evolution and performance change for a 140 mm diameter, three-blade fixed-pitch high-speed propeller. The Rayleigh-Plesset cavitation model, implemented in ANSYS CFX 24.2 within a two-phase Eulerian framework coupled with SST turbulence closure, was used to simulate 72 operational combinations spanning six advance velocities (0–10 m/s) and twelve rotational speeds (300–3600 RPM), with nine representative cases analyzed in detail. Results show that onset thresholds are strongly velocity-dependent: at zero advance velocity (bollard-pull condition, v = 0 m/s) cavitation inception occurs between 1800 and 2000 RPM, whereas at high advance velocity (v = 10 m/s) localized tip-vortex cavitation appears at rotational speeds as low as 300 RPM despite a nominally favourable global cavitation number (σ = 1.96), demonstrating that the global cavitation number alone cannot predict the onset of cavitation. Cavitation consistently initiates at blade tip leading edges, evolving from attached sheet cavitation to supercavitation with vapor fractions exceeding 95% at maximum conditions. At the critical design point, propulsive efficiency reaches η = 38.6% (T = 244.04 N, Q = 12.08 Nm). A direct comparison with the non-cavitating baseline reveals that this effect is regime-dependent: cavitation reduces predicted thrust by 7–11% under bollard pull conditions (v = 0 m/s, 1800–3600 RPM), partially attributable to active-blade-area loss; at moderate advance velocities (v = 2–6 m/s) the two predictions nearly coincide, while at high advance ratio (v = 8–10 m/s) cavitating thrust matches or exceeds the non-cavitating prediction, by up to 88% at v = 10 m/s and 3600 RPM. These findings define indicative operational envelopes, identify blade tip protection as essential for erosion mitigation and provide practical design guidance for high-speed propellers in fast vessels, rescue craft and autonomous surface vehicles. Full article
(This article belongs to the Special Issue Advances in Marine Propulsion Systems and Hydrodynamic Performance)
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22 pages, 4873 KB  
Article
Comparative Analysis of Direct Drop-In Fluid Replacement for a Centrifugal Compression System
by Jordan Dickenson, James R. Bull, Jovana Radulovic and James M. Buick
Processes 2026, 14(15), 2412; https://doi.org/10.3390/pr14152412 - 27 Jul 2026
Viewed by 328
Abstract
The phasing out of high-GWP refrigerants and the growing diversity of working fluids used across heat pumps, refrigeration systems, and closed-cycle power applications have made drop-in fluid replacement a question of significant practical interest. Centrifugal compressors are designed around the thermophysical properties of [...] Read more.
The phasing out of high-GWP refrigerants and the growing diversity of working fluids used across heat pumps, refrigeration systems, and closed-cycle power applications have made drop-in fluid replacement a question of significant practical interest. Centrifugal compressors are designed around the thermophysical properties of a specific fluid, and the performance penalty is incurred when working fluid is replaced without redesigning the impeller. This study presents a CFD comparison of direct drop-in fluid replacement in a fixed geometry centrifugal compression system. Eight working fluids that span the property range relevant to current drop-in substitutions are evaluated: air, nitrogen, argon, carbon dioxide, R22, R134a, R1234yf, and R1234ze(E). A reference centrifugal impeller was reconstructed in ANSYS BladeGen, meshed in ANSYS TurboGrid using the Automatic Topology and Meshing method, and simulated in ANSYS CFX (2024 R2) as a single periodic passage with Frozen Rotor interfaces and Spalart–Allmaras turbulence closure. Performance maps were generated for each fluid across a range of rotational speeds and mass flow rates, with a common inlet reference condition applied across all cases to isolate the influence of fluid properties from an inlet state. The resulting dataset enables a like-for-like comparison of pressure ratio, efficiency, and shaft power requirement, providing a basis for assessing the aerodynamic implications of drop-in fluid substitution in centrifugal compression systems. Air, nitrogen, argon and carbon dioxide achieved similar peak efficiencies (~88%) and comparable pressure ratios (PR), indicating they can be used as drop-in substitutes without performance loss. Refrigerants R1234yf and R1234ze(E) matched R134a in efficiency (peak ~90%) while offering higher pressure ratios and significantly lower power requirements at peak efficiency. At 20,000 RPM and a mass flow rate of 2 kg/s, compared to a PR of 1.45 for air, nitrogen, carbon dioxide and argon achieved PRs of 1.4, 1.9 and 2.4, respectively. At the same settings, R134a and R1234 refrigerants reached PRs of 5 and 6, respectively. The power requirement was ~8 × 104 W for air and similar fluids, and ~11 × 104 W for refrigerants. Full article
(This article belongs to the Special Issue Fluid Dynamics and Thermodynamic Studies in Gas Turbine)
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27 pages, 3592 KB  
Article
Mitigating Particle Erosion in Axial-Flow Turbines Through Air Injection at the Inlet Rotor Section
by José Gustavo Coelho, Rafael de Almeida, Hermeson Conceição Wanzeler and André Luiz Amarante Mesquita
Processes 2026, 14(13), 2218; https://doi.org/10.3390/pr14132218 - 7 Jul 2026
Viewed by 369
Abstract
This study presents a computational analysis of degradation caused by cavitation and hydro-abrasive erosion in a low-head axial microturbine (H=4m), incorporating strategic air injection as a passive mitigation technique. Using Computational Fluid Dynamics (CFD) within ANSYS CFX 2025 [...] Read more.
This study presents a computational analysis of degradation caused by cavitation and hydro-abrasive erosion in a low-head axial microturbine (H=4m), incorporating strategic air injection as a passive mitigation technique. Using Computational Fluid Dynamics (CFD) within ANSYS CFX 2025 R2, the study investigates hydrodynamic performance and the spatial distribution of surface wear across the runner blades. The turbine geometry was developed from aerofoil profiles mapped onto cylindrical coordinates, using a structured three-dimensional mesh with localized refinement to ensure grid independence. Physical modeling employed the Shear Stress Transport (SST) turbulence model, with cavitation dynamics governed by the Rayleigh–Plesset equation and sediment transport modeled using a Lagrangian framework incorporating the Finnie erosion model. The numerical framework showed good agreement with reference characteristic curves, confirming its predictive accuracy. The results indicate that vapor cavities form predominantly on the suction side, whereas solid particle erosion highly concentrated on the pressure side of the blades, where the outer 20% of the span accounts for over 91% of the total erosion intensity. Parametric assessments of controlled air injection revealed a highly non-linear mitigation response, identifying IAVF 2 as the optimal air-injection case. This configuration reduced integrated erosion by 0.95% and maximum localized erosion by 6.17%. In contrast, excessive air volumes accelerated material removal due to localized flow distortion. The findings indicate that carefully controlled air injection is a viable strategy for extending the operational lifespan of small-scale hydropower assets. Full article
(This article belongs to the Special Issue CFD Simulation of Fluid Machinery)
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22 pages, 4763 KB  
Article
Determination of Added-Mass Coefficients in Eccentrically Confined Square Cylinders Using Deforming-Mesh and Immersed-Boundary Methods
by Bruno Oettinger-Barrientos, Armando Blanco-Alvarez and Gonzalo Tampier
Appl. Sci. 2026, 16(11), 5239; https://doi.org/10.3390/app16115239 - 23 May 2026
Viewed by 346
Abstract
Accurate prediction of hydrodynamic forces on confined oscillating structures is essential in applications related to nuclear engineering, energy systems, offshore devices, and mechanical components subjected to flow-induced vibrations. In this work, two computational fluid dynamics (CFD) methodologies implemented in ANSYS CFX are compared [...] Read more.
Accurate prediction of hydrodynamic forces on confined oscillating structures is essential in applications related to nuclear engineering, energy systems, offshore devices, and mechanical components subjected to flow-induced vibrations. In this work, two computational fluid dynamics (CFD) methodologies implemented in ANSYS CFX are compared to determine the added-mass coefficients for a square cross-section cylinder confined within a square container: a deforming-mesh method (DMM) and an immersed-boundary method (IBM). Unlike previous studies restricted either to concentric square cylinders or to eccentric configurations treated with potential flow, the present study addresses eccentric confined configurations by solving the incompressible Navier–Stokes equations and focuses primarily on the prediction of added mass under strong confinement. Horizontal, vertical, and combined eccentric displacements are analyzed in detail. Mesh-independence, domain-size sensitivity, and temporal-convergence analyses are performed. Results show that both methods provide closely matching added-mass predictions over a wide range of eccentricities, with relative differences typically below 1% for moderate eccentricities, although discrepancies increase under extreme confinement. Relative to the concentric configuration, the added-mass coefficient increases by about 44% for the most eccentric vertical case and by about 87% for the most eccentric corner-approach case. Force decomposition and pressure-field analysis show that this increase is governed primarily by pressure-induced inertial effects, whereas viscous shear plays a secondary role under the conditions considered. From a practical standpoint, the immersed-boundary method reduced the computational time by approximately 92% in the most demanding case. Full article
(This article belongs to the Special Issue Mathematical and Numerical Methods in Fluid Engineering)
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47 pages, 9057 KB  
Article
Numerical Investigation of Hydrodynamic–Power Take-Off Coupling in a Modified FOWC Using an Orifice-Based Turbine Surrogate
by A. H. Samitha Weerakoon, Ali Alkhabbaz and Mohsen Assadi
J. Mar. Sci. Eng. 2026, 14(10), 934; https://doi.org/10.3390/jmse14100934 - 18 May 2026
Viewed by 476
Abstract
This study presents a comprehensive numerical investigation of a modified backward bent duct buoy (BBDB) floating oscillating water column (FOWC) system, with emphasis on coupled hydrodynamic response and power take-off (PTO) representation. A fully integrated computational framework is developed using SIEMENS STAR-CCM+, ANSYS [...] Read more.
This study presents a comprehensive numerical investigation of a modified backward bent duct buoy (BBDB) floating oscillating water column (FOWC) system, with emphasis on coupled hydrodynamic response and power take-off (PTO) representation. A fully integrated computational framework is developed using SIEMENS STAR-CCM+, ANSYS AQUA and ANSYS CFX, and three-dimensional CFD, incorporating free-surface wave modeling (VOF), six-degree-of-freedom (6-DOF) body motion, and mooring system interaction under realistic offshore wave conditions (Hs = 3.0 m, T = 9.0 s). A key contribution of this work is the development of an orifice-based PTO surrogate calibrated to replicate turbine-equivalent pressure-drop behavior. Comparative analysis demonstrates that the selected 0.30D orifice reproduces turbine response with deviations below 10% in pressure and flow characteristics, while maintaining superior numerical stability. Hydrodynamic analysis confirms that the modified BBDB-FOWC exhibits stable and bounded motion, with dominant heave-driven response and controlled pitch behavior. The influence of viscous damping is quantified through free-decay analysis and incorporated into the coupled simulations. Results show that damping enhances pressure development by ~25% and flow throughput by ~20%, leading to a significant increase in energy extraction potential. Dimensionless analysis further reveals that the system operates in a turbulent, inertia-dominated regime, governed by nonlinear oscillatory flow dynamics. The combined results demonstrate that the proposed methodology enables accurate, stable, and computationally efficient modeling of floating OWC systems with realistic PTO behavior. The findings provide a scalable framework for future optimization and support the development of high-performance offshore wave energy converters. Full article
(This article belongs to the Special Issue Wave-Driven Ocean Modelling and Engineering)
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22 pages, 17825 KB  
Article
Design and Performance Analysis of a Micro-Axial Compressor for Downhole Boosting
by Jianyi Liu and Jiali Zhu
Appl. Sci. 2026, 16(9), 4294; https://doi.org/10.3390/app16094294 - 28 Apr 2026
Viewed by 587
Abstract
Downhole boosting technology breaks the physical limitations of conventional surface boosting by enhancing pressure at the wellbore bottom, with micro-axial compressors serving as its core compression module. However, traditional axial compressors, when miniaturized, suffer from severe end losses and easy instability, failing to [...] Read more.
Downhole boosting technology breaks the physical limitations of conventional surface boosting by enhancing pressure at the wellbore bottom, with micro-axial compressors serving as its core compression module. However, traditional axial compressors, when miniaturized, suffer from severe end losses and easy instability, failing to adapt to downhole space constraints and the efficient pressurization demands of low-permeability, low-pressure, and small-flow reservoirs. To address this, this study designed a compact micro-axial compressor. CFturbo was used for parametric blade design and optimization, while ANSYS CFX 2025 (with the SST turbulence model) conducted numerical simulations. A “simulation–diagnosis–optimization–validation” closed-loop strategy was adopted to adjust the blade’s leading-edge shape, camber line, and thickness distribution, combined with grid independence verification and inter-stage matching optimization. The results show that at the design speed (60,000 rpm), the compressor achieves a pressure ratio of 1.57 and an isentropic efficiency of 83.6%. It also maintains stable performance at 55,000 rpm (off-design speed), with excellent inter-stage aerodynamic matching and controllable leakage losses. This compressor meets downhole operational needs, providing technical support for developing low-permeability, low-pressure, small-flow reservoirs. Full article
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24 pages, 17063 KB  
Article
Aerodynamic Effect of Gurney Flaps on NREL Phase VI Wind Turbine Blade
by Asaad Hanoon, Ziaul Huque, Raghava Rao Kommalapati, Mst Sumaiya Akter Snigdha, Khadiza Akter Keya and Kenneth Oluwatobi Fadamiro
Wind 2026, 6(2), 19; https://doi.org/10.3390/wind6020019 - 21 Apr 2026
Viewed by 983
Abstract
As the population increases, the demand for power continues to rise. As fossil fuel resources reduce, wind energy emerges as a sustainable alternative and helps address adverse effects of global warming and environmental pollution caused by fossil fuels. Thus, this study focuses on [...] Read more.
As the population increases, the demand for power continues to rise. As fossil fuel resources reduce, wind energy emerges as a sustainable alternative and helps address adverse effects of global warming and environmental pollution caused by fossil fuels. Thus, this study focuses on increasing the efficiency of wind turbines by improving their energy conversion. In this study, the NREL Phase VI wind turbine blade was modified by adding a Gurney flap at trailing edge along the entire span. Computational fluid dynamics simulations using ANSYS CFX 19.2 were performed on the modified blades to evaluate their aerodynamic performance. Three different flap lengths were investigated with six wind speeds varying from 5 m/s to 20 m/s. The results obtained were compared with those from NREL Phase VI original shape and a blade equipped with a winglet. Computational domain was divided into a rotating cylindrical region and a stationary rectangular part. The aerodynamic parameters calculated include torque, thrust, and normal and tangential forces coefficients. At low velocities, the addition of a Gurney flap had an insignificant impact on torque and thrust, whereas at medium to high wind speeds, significant increases were observed on torque, indicating more power production. Full article
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15 pages, 670 KB  
Article
Genomic and Plasmid Distribution of Clinically Relevant Antibiotic Resistance Genes in Pets and Free-Roaming Dogs in an Urban Area of Central Mexico
by María Daniela Frade-Pérez, Rosa Martha Pérez-Serrano, Sergio Francisco Cornelio-Martínez, María Laura González-Dávalos, Yezenia Rubio-Venegas, Enrique Flores-Gasca, Alfredo Varela-Echavarría and María Ofelia Mora-Izaguirre
Pets 2026, 3(1), 15; https://doi.org/10.3390/pets3010015 - 15 Mar 2026
Viewed by 1588
Abstract
Dogs, especially as pets but also an increasing number of stray dogs, share environments with humans, facilitating the transfer of antibiotic resistance genes (ARGs) between genetic compartments, with zoonotic and public health implications that must be addressed within One Health. In this cross-sectional [...] Read more.
Dogs, especially as pets but also an increasing number of stray dogs, share environments with humans, facilitating the transfer of antibiotic resistance genes (ARGs) between genetic compartments, with zoonotic and public health implications that must be addressed within One Health. In this cross-sectional comparative study, we explored the distribution of seven selected clinically relevant ARGs in both genomic DNA (gDNA) and plasmid DNA (pDNA), and the phenotypic resistance profile of the cultivable microbiota, between pet dogs (PeDs, n = 12) and free-roaming dogs (FRDs, n = 10) in Mexico. Tetracycline resistance genes (tetQ, tetW, and tetM) predominated in both compartments (40% to 100%), suggesting the presence of a core tetracycline-associated resistome. In contrast, plasmid-associated differences were group-specific: in pDNA cfxA was enriched in FRDs (90%) and tetK in PeDs (42%), whereas blaTEM-1 and ermC were absent in two dog populations. Cultivable bacteria from both groups exhibited phenotypic multidrug resistance, particularly by β-lactams, macrolides, lincosamides, and tetracyclines. FRDs also harbored pathogenic–zoonotic bacteria such as Yersinia enterocolitica, Campylobacter jejuni, and Enterococcus faecalis. Our findings indicated that FRDs and PeDs harbor substantial resistomes, with differences in plasmid-associated ARGs, revealing a transfer potential related to environmental exposure. Full article
(This article belongs to the Topic Research on Companion Animal Nutrition)
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35 pages, 18152 KB  
Article
Empirical Energy Dissipation Model for Variable-Slope Three-Section Stepped Spillways Validated Through Dimensional Analysis and CFD Simulation
by Luis Antonio Yataco-Pastor, Ana Cristina Ybaceta-Valdivia, Yoisdel Castillo Alvarez, Reinier Jiménez Borges, Luis Angel Iturralde Carrera, José R. García-Martínez and Juvenal Rodríguez-Reséndiz
Fluids 2026, 11(3), 78; https://doi.org/10.3390/fluids11030078 - 13 Mar 2026
Viewed by 1357
Abstract
Energy dissipation in stepped weirs depends on the complex interaction between geometry, flow regime, and surface aeration. The research proposes a dimensionless empirical model (RE3T) to predict the overall energy dissipation in three-section stepped weirs with variable slopes. The formulation integrates dimensional analysis [...] Read more.
Energy dissipation in stepped weirs depends on the complex interaction between geometry, flow regime, and surface aeration. The research proposes a dimensionless empirical model (RE3T) to predict the overall energy dissipation in three-section stepped weirs with variable slopes. The formulation integrates dimensional analysis based on the Vaschy–Buckingham theorem, controlled physical experimentation, and three-dimensional numerical simulations using CFD employing the RANS–SST turbulence model implemented in ANSYS CFX. Eighteen numerical simulations were performed covering seven geometric configurations and four hydraulic inlet conditions, covering slug, transitional, and skimming flow regimes. The CFD model was previously validated by comparison with a physical scale model, obtaining a discrepancy of only 0.38% in relative energy dissipation. The validated dataset was then used to calibrate an empirical multiplicative correlation composed of eight dimensionless groups associated with sectional slopes, number of steps, overall geometric ratio, and upstream Froude number. The proposed model achieved a coefficient of determination R2 = 0.81, with relative errors generally less than 1% and a maximum deviation of 2.34%. The statistical indicators (RMSE, MAE, and bias) confirm the absence of significant systematic trends within the defined domain of validity. The results show that the Froude number and the slopes of the sections are the variables with the greatest influence on overall dissipation. The RE3T formulation is a physically consistent and computationally efficient predictive tool for the design and analysis of stepped weirs with variable slopes, extending the scope of traditional correlations developed for uniform slopes. Full article
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22 pages, 13217 KB  
Article
Analysis of the Gas–Liquid Two-Phase Flow Characteristics of Multistage Centrifugal Pumps Under Different Rotational Speeds
by Yongfei Yang, Lu Chen, Weidong Shi, Linwei Tan, Yupeng Cao, Rui Zhou, Yu Lu and Chunhui Ma
Water 2026, 18(6), 652; https://doi.org/10.3390/w18060652 - 10 Mar 2026
Cited by 2 | Viewed by 879
Abstract
Performance deterioration and unstable operation are common when multistage centrifugal pumps handle gas–liquid mixtures. Here, we investigate a two-stage centrifugal pump over a wide speed range and inlet gas volume fractions (IGVFs) using experiments and CFD. The two-phase flow is simulated with a [...] Read more.
Performance deterioration and unstable operation are common when multistage centrifugal pumps handle gas–liquid mixtures. Here, we investigate a two-stage centrifugal pump over a wide speed range and inlet gas volume fractions (IGVFs) using experiments and CFD. The two-phase flow is simulated with a Eulerian–Eulerian two-fluid approach (liquid as the continuous phase; gas as a dispersed bubbly phase with a representative bubble diameter of 0.3 mm). Turbulence is closed using the SST k–ω model for the liquid phase and the built-in dispersed-phase turbulence treatment in ANSYS CFX. Transient pressure signals are analyzed in the time and frequency domains (FFT) to assess how rotational speed affects void-fraction distribution, overall performance, and the dominant unsteady components within the adopted modeling framework. The results show that IGVF primarily controls gas accumulation in the impeller passages: as IGVF increases, the gas phase evolves from dispersed bubbles to a central core, whereas speed mainly alters the detailed morphology via centrifugal effects. Similarity-law scaling is strongly speed-dependent in this pump: agreement is better at higher speeds and deteriorates at lower speeds where viscous effects become more influential. The dominant unsteady content also changes with speed, shifting from low-speed broadband features associated with gas redistribution to high-speed periodic components linked to blade–vane rotor–stator interaction (RSI). In addition, the downstream stage exhibits more uniform void fraction and more regular periodic signatures, consistent with an inter-stage flow-rectification effect. These observations provide practical guidance for hydraulic design and variable-speed operation of multistage pumps under gas entrainment. Full article
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16 pages, 2196 KB  
Article
Extracellular Metabolite Profiling in CO2-Fixing Bacterium Rhodobacter sphaeroides Under Autotrophic Conditions
by Yu Rim Lee, Suhyeon Hong, Young-Hwan Chu, Soo Youn Lee and Sangmin Lee
Metabolites 2026, 16(3), 156; https://doi.org/10.3390/metabo16030156 - 26 Feb 2026
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Abstract
Background/Objectives: Rhodobacter sphaeriids is considered a promising biomanufacturing platform due to its capacity to convert CO2 into value-added products. To enhance the yield of CO2-derived products, understanding extracellular metabolite dynamics during autotrophic growth is essential. However, the extracellular metabolite [...] Read more.
Background/Objectives: Rhodobacter sphaeriids is considered a promising biomanufacturing platform due to its capacity to convert CO2 into value-added products. To enhance the yield of CO2-derived products, understanding extracellular metabolite dynamics during autotrophic growth is essential. However, the extracellular metabolite profiles of R. sphaeroides under autotrophic conditions have not been reported. Methods: In this study, we performed a comprehensive analysis of extracellular metabolites produced under autotrophic conditions using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) and liquid chromatography time-of-flight mass spectrometry (LC-TOFMS). Results: A total of 62 putative metabolites were detected, of which 23 were measured above the quantification limit. Metabolites involved in glycolysis and gluconeogenesis constituted the largest proportion of extracellular metabolites, with lactic acid exhibiting the highest accumulation levels. To investigate the transcriptional changes associated with metabolite accumulation, we analyzed gene expression and observed the downregulation of glycolytic genes, including pgi, gapB, and lctB, whereas cfxA, encoding fructose-1,6-bisphosphate aldolase, was upregulated under autotrophic conditions compared to heterotrophic conditions. Conclusions: These results suggest that the carbon assimilation metabolic flux in R. sphaeroides shifts toward the CBB cycle and lactic acid overflow metabolism under autotrophic conditions. Collectively, these findings provide new insights into metabolic regulation during autotrophic growth and offer a basis for reducing extracellular byproduct formation and improving CO2-based biological production in R. sphaeroides. Full article
(This article belongs to the Section Microbiology and Ecological Metabolomics)
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