Search Results (39,859)

This study investigates the functional role of OsHSBP1, a heat shock factor-binding protein, in regulating abiotic stress tolerance in rice, with the aim of enhancing climate resilience in the elite indica cultivar Bacthom 7 (BT7). Using Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9) genome editing, we generated transgene-free homozygous knockout lines targeting OsHSBP1 and evaluated their physiological, biochemical, and agronomic responses under heat stress. Mutant lines exhibited markedly improved tolerance to both stresses, with survival rates reaching 43–46% under heat stress, compared to near-zero in wildtype plants. Enhanced tolerance was associated with significantly increased catalase and peroxidase activities and reduced oxidative damage, including lower malondialdehyde content and decreased superoxide accumulation. Despite these stress-related advantages, the knockout lines showed minimal differences in key agronomic traits under normal growing conditions, with comparable plant height, tillering ability, grain yield, and amylose content relative to the wildtype. These results demonstrate that OsHSBP1 functions as a negative regulator of abiotic stress tolerance in rice, and its knockout enhances resilience without compromising yield potential. The study highlights OsHSBP1 as a promising target for precision breeding of climate-resilient rice cultivars. Full article

Adventitious shoot regeneration is an essential prerequisite for the application of biotechnological tools such as CRISPR-Cas in woody fruit crops. Nonetheless, many Prunus species exhibit strong recalcitrance to in vitro regeneration. Light quality has emerged as an important environmental factor influencing morphogenic responses under in vitro conditions. In this study, the effect of different LED light spectra on adventitious shoot regeneration was evaluated in three peach-related genotypes: the commercial peach cultivar ‘Siroco 5’ (Prunus persica L.) and the hybrid rootstocks ‘GF677’ and ‘Garnem’ (P. persica × P. dulcis). Callus explants derived from the basal region of in vitro proliferation cultures were exposed for 30 days to five LED light treatments: white (control), blue, red + far-red, mixed (red + far-red + blue), and sequential LED light. Regeneration efficiency was assessed through the frequency of organogenic callus formation (FOC), the number of regenerated shoots per explant, the organogenic rate, and the fresh weight of the regenerated explants. While FOC was consistently high across genotypes and light treatments, shoot regeneration was significantly influenced by both genotype and light spectrum. The hybrid rootstocks exhibited a higher regeneration capacity than the commercial cultivar under most conditions. Red + far-red LED light promoted the highest regeneration efficiency across all of the genotypes, particularly enhancing shoot regeneration and fresh weight in ‘Siroco 5’. These results demonstrate that LED light spectrum modulation, especially red + far-red, is an effective strategy to optimize adventitious shoot regeneration in peach cultivar and hybrid rootstock genotypes, providing a robust basis for future applications in micropropagation and genetic improvement programs. Full article

Background/Objectives: Alterations of the phosphatidylinositol 3-kinase catalytic subunit alpha gene (PIK3CA) are identified in approximately 2–4% of non-small cell lung cancer (NSCLC) cases; however, their biological and clinical relevance in NSCLC remains incompletely understood. This study aimed to comprehensively characterize the clinical and molecular features, as well as outcomes, of patients with PIK3CA-altered NSCLC across different disease stages. Methods: We conducted a retrospective multicenter analysis of 62 patients with histologically confirmed early-stage or advanced NSCLC-harboring PIK3CA alterations (mutations and/or gene amplifications) treated between 2015 and 2022 at three Italian institutions. Demographic, clinical, pathological, and molecular variables were systematically collected and analyzed. Results: PIK3CA mutations accounted for the majority of alterations (90.3%), while amplifications represented 9.7%. The most frequent mutations involved exon 9 (66.1%), predominantly E545K and E542K, followed by exon 20 (16.1%). Most patients were current or former smokers, and concomitant oncogenic alterations were detected in 59.7% of cases, most commonly KRAS mutations. A history of prior malignancy was reported in 24.6% of cases. In the metastatic setting, adenocarcinoma histology was associated with significantly longer overall survival (OS) compared with non-adenocarcinoma histologies (18.4 vs. 5.5 months; p = 0.02). Patients with PD-L1–negative tumors demonstrated a numerically longer OS than those with PD-L1–positive tumors; however, this difference did not reach statistical significance (19.1 vs. 5.4 months; p = 0.05). No statistically significant survival differences were observed according to specific PIK3CA mutation subtypes or treatment strategies. Conclusions: PIK3CA-altered NSCLC represents a molecularly heterogeneous and clinically understudied subgroup, frequently characterized by co-occurring oncogenic alterations. In this study, no definitive prognostic or predictive role for PIK3CA alterations could be established. Nevertheless, these findings provide a descriptive real-world characterization of this molecular subset and support the need for validation in larger, prospectively designed, molecularly stratified studies. Full article

Titanium alloys are widely used as bone graft materials due to their excellent corrosion resistance and biocompatibility. Implant failure can result from long-term exposure to body fluids and inflammation-induced pH decreases, both of which compromise the material’s corrosion resistance and mechanical stability. To address this issue, porous Ti-6Al-4V alloy was selected in this work. Immersion tests were conducted in Hank’s solution with different pH values (3, 5, and 7) for 90 days to simulate the in vivo microenvironment under various physiological conditions. The degradation behavior of porous Ti-6Al-4V alloy during the 90-day immersion period was systematically investigated using a combination of characterization techniques. The results indicated that TiO₂, Ca₃(PO₄)₂, and Ca(H₂PO₄)₂ phases were formed on the surface of the after 90 days of immersion. Massive dissolution of TiO₂ was observed in solutions with high H⁺ concentration (low pH). Ion release tests revealed that the concentration of titanium ions released was significantly higher in acidic solutions, suggesting that the passive film formed on porous Ti-6Al-4V alloy was unstable and prone to dissolution under acidic conditions. Consequently, a large amount of corrosion products accumulated on the specimen surfaces immersed in acidic solutions for a long duration. Moreover, the compression properties of the samples deteriorated after immersion. Specifically, the compressive strength decreased by 12.68 MPa, 11.67 MPa, and 5.84 MPa for sample immersed in solutions with pH = 3, 5, and 7, respectively. The significant reduction in compressive performance of the alloy in high H⁺ concentration solutions was attributed to the decreased compactness caused by ion release. The fracture mode of the porous Ti-6Al-4V alloy after immersion was identified as a mixed mode of ductile and brittle fracture. Full article

In order to solve the problems of low calorific value and pipeline corrosion caused by high concentration of CO₂ in oil-associated gas, and promote the resource utilization of associated gas, this study used validated grand canonical Monte Carlo (GCMC) and molecular dynamics (MD) simulation to investigate the adsorption characteristics of 11 different topological structures (straight-channel MFI/BEA, cage-channel LTA/FAU/CHA) and cation types (Ca²⁺, Na⁺, H⁺) of commercial zeolites for CO₂ and alkanes (CH₄, C₂H₆, C₃H₈) at 0%~90% RH. The results showed that the CO₂ adsorption capacity of all zeolites decreased with increasing humidity, but straight-channel zeolites (ZSM5-300, BETA-25) had excellent moisture resistance, with only a 20.8% and 30.6% decrease in capacity at 90% RH, respectively. The performance of cage-channel zeolite drops sharply under high humidity. Topology structure and cation synergistically regulate separation efficiency, maintaining stable diffusion order in straight channels. Ca²⁺ enhances dry state capacity but is prone to hydrophilic failure. The adsorption heat of CO₂ on straight-channel zeolite is 25–38 kJ/mol, resulting in lower regeneration energy consumption. ZSM5-300 is preferred for PSA (CH₄/CO₂ kinetic separation coefficient of 809.52 at 90% RH), and NaFAU is preferred for TSA (CO₂ adsorption capacity of 3.6 mmol/g and selectivity of 502.6 at 90% RH). This study clarifies the core structure-activity relationship and provides key theoretical support for the decarbonization of oil-associated gas. Full article

Ocimum (basil) is a globally significant medicinal and culinary herb. While its bioactive secondary metabolites are well-studied, the medicinal potential of its abundant primary metabolites (amino acids, vitamins, carbohydrates, steroids) remains largely unexplored. To address this gap, we employed an integrated multi-omics strategy. First, UPLC-MS/MS-based metabolomics quantified primary metabolites across six distinct Ocimum accessions (Ocimum × africanum, Ocimum tenuiflorum, Ocimum gratissimum). Profiling identified 291 primary metabolites, revealing significant interspecific variation, with 273 differential accumulated metabolites (DAMs). Subsequent network pharmacology analysis of 61 high-impact DAMs predicted 516 potential targets. Protein–protein interaction refinement yielded 28 core targets, predominantly integrins (ITGB1, ITGB3, ITGA4, ITGA2B, ITGAV) and kinases (IGF1R, PIK3CA, SRC). Enrichment analysis implicated these targets in focal adhesion, ECM-receptor interaction, and PI3K-Akt signaling pathways. Molecular docking confirmed strong potential binding (binding energy < −7 kcal/mol) between key tripeptides (e.g., Met-Ser-Tyr, Phe-Cys-Gln) and integrin subunits. Antioxidant assays (DPPH, ABTS, FRAP) further showed significant genotypic variation. This study systematically deciphers the primary metabolome of Ocimum and, through a multi-omics approach, reveals novel integrin-mediated mechanisms underpinning its potential therapeutic value, providing a foundation for developing basil-based nutraceuticals and pharmaceuticals. Full article

Al-Musk Lake, an artificial waterbody of 2.9 km² formed by illegal dumping of 9.5 million cubic meters of raw sewage near Jeddah, Saudi Arabia, remains a significant subsurface environmental hazard after drainage activities in 2010. The current research employs a multidisciplinary approach, integrating geological mapping, aeromagnetic and electromagnetic surveys, Landsat imagery, and chemical analyses, to investigate contamination migration and accumulation. The objective is to delineate subsurface contamination pathways and assess their impact on soil and groundwater quality. Frequency-domain electromagnetic (FDEM) surveys identified areas of high apparent conductivity (up to 200 mS/m at 2000 kHz), indicative of deep contamination saturation. Chemical analysis of water and soil samples revealed distressing levels of heavy metals, Na⁺ up to 2400 mg/L, Ca²⁺ up to 3648 mg/L, and Fe up to 4150 mg/L, far exceeding irrigation safe standards. Findings locate two at-risk areas several kilometers from the lake, where contaminants accumulate through basement depressions controlled by faults. These pose immediate risks to adjacent residential areas and expanding agricultural belts. In short, subsurface contamination continues to spread westward. Short-term remedies include halting agricultural activities, treating in-storage water, and paving infiltration zones. A larger-scale geophysical survey, along with denser geochemical sampling and analysis, is necessary to guide long-term remediation and to protect public health. Full article

The manufacturing process of ordinary Portland cement (OPC) is highly resource-intensive and significantly contributes to global CO₂ emissions, thereby exacerbating global warming. In this context, researchers are progressively adopting geopolymer concrete owing to its environmentally friendly production process. However, cracks in OPC and geopolymer concrete structures can substantially reduce their lifespan by exposing reinforcement to the external environment, resulting in concrete deterioration. To mitigate these issues, the self-healing capability of concrete presents an innovative solution to restore structural integrity and minimise maintenance costs. This research delineates various healing techniques and their efficacy for geopolymer concrete, including crystalline admixture, fibres, bacteria, and enzymes. This study primarily examines geopolymer compositions to assess the self-healing efficiency of different healing agents. As many healing agents, including crystalline admixtures and enzyme-based systems, were originally developed for OPC-based concrete and remain underexplored in geopolymers, parallel investigations on OPC systems are also conducted to enable a comparative understanding of the underlying healing mechanisms. The current state of research indicates that crystalline admixture was unable to facilitate crack healing within the geopolymer matrix unless an additional 10% Ca(OH)₂ was incorporated into the binder. The inclusion of fibres embedded with healing agents markedly improved the healing efficiency, achieving a crack width of up to 800 µm when utilised with natural fibres and bacteria. The integration of an optimal quantity of various healing agents enhances the compressive, split tensile, and flexural strength of the concrete. The optimal dosages for the crystalline admixture ranged from 1% to 1.5% by weight of the binder, while the concentration of bacteria ranged from 10⁵ to 10⁷ cells/mL. Furthermore, this review delineates the practical applications and limitations of various healing agents. By integrating appropriate healing agents into geopolymer concrete, this research aims to advance a sustainable approach to durable infrastructure. Full article

Most transient receptor potential (TRP) channels are Ca²⁺-permeable non-selective cation channels that function as polymodal receptors activated by a wide variety of stimuli, including natural compounds such as pungent substances, physical stimuli, lipids, intracellular signaling molecules, and ions. Their physiological roles are diverse, including sensory perception, ion transport, and intracellular signaling. Similarly, Piezo channels, which are also Ca²⁺-permeable non-selective cation channels, are activated by mechanical stimuli such as membrane stretching and contribute to touch sensation, blood flow regulation, and bladder-filling sensation, among other functions. While research on non-selective cation channels in relation to energy metabolism has primarily focused on TRP channels expressed in primary afferent neurons, studies over the past decade have revealed the important roles of TRP and Piezo channels in brown adipocytes. In this review, we highlight evidence regarding the contributions of TRPV2 and Piezo1 to brown adipocyte differentiation and thermogenesis and briefly summarize recent advances regarding other TRP channels expressed in brown adipocytes. Furthermore, we propose a conceptual framework in which a “modal shift” in TRP/Piezo channels, defined as developmental stage-dependent changes in their functional properties, may contribute to the regulation of brown adipocytes’ functions. Full article

As a major retrovirus threatening global poultry farming, Avian Leukosis Virus Subgroup J (ALV-J) has expanded its host range since discovery, extending from conventional broilers to layer chickens and native breeds. Its diverse oncogenic manifestations, including myeloid leukemia, hemangiomas, and tumors of immune and visceral organs, have led to increased mortality, reduced productivity, and substantial economic losses in the poultry industry. Based on the current body of literature, this review summarizes and synthesizes advances in the etiological characteristics, infection and pathogenic mechanisms, host resistance, and research progress in prevention and control of ALV-J. Accumulating evidence indicates that viral evolution driven by mutations and recombination—particularly in the env gene and LTR regions—plays a central role in host range expansion, tumor diversity, and immune evasion. Current studies consistently demonstrate that host resistance to ALV-J is a multifactorial process involving genetic polymorphism, innate immune responses, and cellular autonomous defense systems. In this context, recent advances in disease-resistant breeding highlight CRISPR-Cas9-mediated gene editing as a promising strategy for blocking viral entry or replication. Despite these advances, major gaps remain, including an incomplete understanding of virus–host interaction networks, limited insight into co-infection-mediated synergistic pathogenicity, the absence of effective vaccines, and insufficient large-scale epidemiological surveillance and purification systems. Addressing these challenges will be critical for the development of integrated prevention strategies and the sustainable control of ALV-J in poultry production. Full article

Edible films are increasingly recognized as promising sustainable packaging alternatives, but often face challenges such as poor mechanical strength, limited barrier properties, and low oxidative stability. This study aimed to enhance the physicochemical performance of collagen fiber–starch composite films by incorporating polyphenols (including tannic acid (TA), caffeic acid (CA), and their oxidized forms, OTA and OCA) as natural cross-linkers and antioxidants. Results showed that the addition of 0.1% TA increased the tensile strength by approximately 45% compared to the control, while simultaneously reducing the water vapor permeability from 1.32 to 1.26 g·mm/kPa·h·m², with TA outperforming CA due to its higher molecular weight and stronger intermolecular interactions. Oxidized polyphenols further improved the mechanical and water vapor barrier properties via quinone-induced covalent cross-linking, thereby forming a denser film network. The films also exhibited enhanced UV–visible light shielding, with nearly complete ultraviolet blockage (transmittance is close to zero in the 200–280 nm range). Non-oxidized polyphenols showed higher antioxidant activity in the ABTS and reducing power assays, while release kinetics analysis revealed the highest release rate in 50% ethanol, indicating a pronounced solvent-dependent behavior. Specifically, films with 0.1% TA exhibited an ABTS radical scavenging activity of over 80%, significantly higher than the control. Overall, polyphenols effectively improve film performance through cross-linking and structural modification, offering a theoretical foundation for designing active packaging for targeted food systems. Full article

Micro-expressions (MEs) are involuntary facial movements that reveal genuine emotions, holding significant value in fields like deception detection and psychological diagnosis. However, micro-expression recognition (MER) is fundamentally challenged by the entanglement of subtle emotional motions with identity-specific features. Traditional methods, such as those based on Robust Principal Component Analysis (RPCA), attempt to separate identity and motion components through fixed preprocessing and coarse decomposition. However, these methods can inadvertently remove subtle emotional cues and are disconnected from subsequent module training, limiting the discriminative power of features. Inspired by the Bruce–Young model of facial cognition, which suggests that facial identity and expression are processed via independent neural routes, we recognize the need for a more dynamic, learnable disentanglement paradigm for MER. We propose LFD-TCMEN, a novel network that introduces an end-to-end learnable feature disentanglement framework. The network is synergistically optimized by a multi-task objective unifying orthogonality, reconstruction, consistency, cycle, identity, and classification losses. Specifically, the Disentangle Representation Learning (DRL) module adaptively isolates pure motion patterns from subject-specific appearance, overcoming the limitations of static preprocessing, while the Temporal-Complemented Motion Enhancement (TCME) module integrates purified motion representations—highlighting subtle facial muscle activations—with optical flow dynamics to comprehensively model the spatiotemporal evolution of MEs. Extensive experiments on CAS(ME)³ and DFME benchmarks demonstrate that our method achieves state-of-the-art cross-subject performance, validating the efficacy of the proposed learnable disentanglement and synergistic optimization. Full article

This study investigates the thermodynamic and experimental aspects of producing a chromium–manganese ligature under high-temperature smelting conditions using low-grade iron–manganese ore and ferrosilicochrome (FeSiCr) dust as both a reducing agent and a chromium source. Thermodynamic modeling of the multicomponent Fe–Cr–Mn–Si–Al–Ca–Mg–O system was carried out using the HSC Chemistry 10 and FactSage 8.4 software packages to substantiate the temperature regime, reducing agent consumption, and conditions for the formation of a stable metal–slag system. The calculations indicated that efficient reduction of manganese oxides and formation of the metallic phase are achieved at a smelting temperature of 1600 °C with a reducing agent consumption of approximately 50 kg. Experimental smelting trials conducted in a laboratory Tammann furnace under the calculated parameters confirmed the validity of the thermodynamic predictions and demonstrated the feasibility of obtaining a concentrated chromium–manganese ligature. The resulting metallic product exhibited a high total content of alloying elements and had the following chemical composition (wt.%): Fe 35.41, Cr 41.10, Mn 8.15, and Si 4.31. SEM–EDS microstructural analysis revealed a uniform distribution of chromium and manganese within the metallic matrix, indicating stable reduction behavior and favorable melt crystallization conditions. The obtained results demonstrate the effectiveness of an integrated thermodynamic–experimental approach for producing chromium–manganese ligatures from low-grade mineral raw materials and industrial by-products and confirm the potential applicability of the proposed process for complex steel alloying. Full article

Driven by rapid socioeconomic progress and changing lifestyles, the global burden of cardiovascular diseases (CVDs) continues to escalate, with surging morbidity and mortality rates imposing a severe threat to public health. Clinical treatments are focused on the alleviation of treatments, highlighting the need for a deeper understanding of CVDs pathogenesis and the development of targeted therapies. Recent studies have identified imbalances in intracellular Ca²⁺ homeostasis as a key pathological mechanism in the progression of CVDs. Notably, sarcoplasmic/endoplasmic reticulum Ca²⁺-ATPase 2 (SERCA2), a membrane protein encoded by the ATP2A2 gene and ranging from 97 to 115 kDa in molecular weight, plays a pivotal role in regulating intracellular Ca²⁺ levels. Extensive evidence links abnormal SERCA2 function to various CVDs, including heart failure, cardiac hypertrophy, atherosclerosis, and diabetic cardiomyopathy. This review systematically explores the regulatory mechanisms of SERCA2 expression and its functional regulation—including transcriptional regulation, post-translational modifications, and protein–protein interactions—and further investigates its pathological roles in cardiovascular diseases as well as its potential as a therapeutic target. By synthesizing current knowledge, this article aims to provide new insights for future basic research and establish a theoretical foundation for clinical applications. Full article

Pathogens causing candidiasis encompass a diverse group of ascomycetous yeasts that have become essential models for studying fungal adaptability, pathogenicity, and host–pathogen interactions. Although many candidiasis-promoting species exist as commensals within host microbiota, several have acquired virulence traits that enable opportunistic infections, positioning them as a leading cause of invasive fungal disease in humans. Deciphering the molecular and genetic determinants that underpin the biology of organisms responsible for candidiasis has long been a central objective in medical and molecular mycology. However, research progress has been constrained by intrinsic biological challenges, including noncanonical codon usage and the absence of a complete sexual cycle in diploid species, which have complicated traditional genetic manipulation. CRISPR-Cas9 genome editing has overcome many of these limitations, providing a precise, efficient, and versatile framework for targeted genomic modification. This system has facilitated functional genomic studies ranging from single-gene deletions to high-throughput mutagenesis, yielding new insights into the mechanisms governing virulence, antifungal resistance, and stress adaptation. Since its initial application in Candida albicans, CRISPR-Cas9 technology has been refined and adapted for other clinically and industrially relevant species, including Nakaseomyces glabratus (formerly referred to as Candida glabrata), Candida parapsilosis, and Candida auris. The present work provides an overview of the evolution of genetic approaches employed in research directed against candidiasis-associated species, with a particular focus on the development and optimization of CRISPR-based systems. It highlights how recent advancements have improved the genetic tractability of these pathogens and outlines emerging opportunities for both fundamental and applied studies in fungal biology. Full article