Search Results (40)

Snails at hydrothermal vents rely on symbiotic bacteria for nutrition; however, the specifics of these associations in adapting to such extreme environments remain underexplored. This study investigated the community structure and metabolic potential of bacteria associated with two Indian Ocean vent snails, Chrysomallon squamiferum and Gigantopelta aegis. Using microscopic, phylogenetic, and metagenomic analyses, this study examines bacterial communities inhabiting the foot and gland tissues of these snails. G. aegis exhibited exceptionally low bacterial diversity (Shannon index 0.14–0.18), primarily Gammaproteobacteria (99.9%), including chemosynthetic sulfur-oxidizing Chromatiales using Calvin–Benson–Bassham cycle and methane-oxidizing Methylococcales in the glands. C. squamiferum hosted significantly more diverse symbionts (Shannon indices 1.32–4.60). Its black variety scales were dominated by Campylobacterota (67.01–80.98%), such as Sulfurovum, which perform sulfur/hydrogen oxidation via the reductive tricarboxylic acid cycle, with both Campylobacterota and Gammaproteobacteria prevalent in the glands. The white-scaled variety of C. squamiferum had less Campylobacterota but a higher diversity of heterotrophic bacteria, including Delta-/Alpha-Proteobacteria, Bacteroidetes, and Firmicutes (classified as Desulfobacterota, Pseudomomonadota, Bacteroidota, and Bacillota in GTDB taxonomy). In C. squamiferum, Gammaproteobacteria, including Chromatiales, Thiotrichales, and a novel order “Endothiobacterales,” were chemosynthetic, capable of oxidizing sulfur, hydrogen, or iron, and utilizing the Calvin–Benson–Bassham cycle for carbon fixation. Heterotrophic Delta- and Alpha-Proteobacteria, Bacteroidetes, and Firmicutes potentially utilize organic matter from protein, starch, collagen, amino acids, thereby contributing to the holobiont community and host nutrition accessibility. The results indicate that host species and intra-species variation, rather than the immediate habitat, might shape the symbiotic microbial communities, crucial for the snails’ adaptation to vent ecosystems. Full article

The objective of the study is to demonstrate how cognitive chief executive officers (CEOs) influence corporate social and environmental performance under the moderating impact of innovation, supervisors and cash holdings. Significantly, we have formulated cognitive CEOs using data envelope analysis while considering the specific attributes of the incumbent CEO (by considering the age, tenure, goodwill, education and tacit knowledge of CEOs). The research approach aims to elucidate that cognitive CEOs strongly invigorate social and environmental performance. However, the moderating role of corporate innovation weakens this connection, whereas the moderating role of supervisors invigorates this relationship. In contrast, cash hoarding deters social and environmental performance through its moderating effectiveness. Conclusively, theoretical contribution illuminates the stakeholder theory frame of reference while emphasizing the identification of corporate social and environmental performance. Specifically, the role of cognitive CEOs has been signified as a promoter of such strategies, which indicate their orientation toward social responsibility. Empirical underpinnings illustrate the impact of corporate innovation, supervisors and cash holdings, which asymmetrically influence social and environmental performance. The recommendations of the research results refer to the characterization of the optimal attributes of cognitive CEOs that are essential to enhance social and environmental performance. Full article

The first few days of the second Trump presidency saw a flurry of executive orders targeting immigrants, the transgender community, blacks, and federal government employees. The resulting “shocks” to the system are unprecedented, causing many to question the continuation of American democracy. These actions are a few of many policy preferences developed by conservatives and captured in “Project 2025”, spearheaded by the Heritage Foundation and with the overall intent of restructuring the federal government to suit authoritarian aims. Using a theoretical framework regarding policy entrepreneurs (PEs), I argue that conservative PEs (CPEs), under the aegis of the Republican Party and a second Trump term, are encouraging a shift away from a federal democratic republic to an authoritarian regime. CPEs of interest include elected officials, think tanks, media outlets, interest groups, and political operatives; collectively, these entities constitute a network of mostly unnoticed conservative political professionals using the mechanisms of policy entrepreneurship to damage American democracy. Overall, the actions CPEs take in terms of setting the agenda, controlling the electoral process, and informing the media indicate an eventual abandonment of democratic norms, an inclination towards the establishment of an authoritarian regime, and acceptance of said regime by a significant portion of the American people. Full article

This study evaluates the economic, social, environmental, and institutional impacts of the Geographical Indication (GI) of the Indication of Origin (IO) type in the Abaíra Microregion, Bahia, Brazil, and its alignment with the Sustainable Development Goals (SDGs), particularly SDGs 2, 4, 8, 12, and 16. The research focuses on assessing the long-term sustainability effects of the Abaíra GI on local communities and territories after its recognition in 2014. Using an exploratory, qualitative approach, this study applies the Economic, Social, and Environmental Impact Assessment Methodology for GIs developed by EMBRAPA, along with semi-structured interviews and field visits with local producers and cooperative members. The analysis highlights the importance of post-GI sustainability assessment and identifies significant impacts on local economic development, cultural preservation, and environmental management. The research reveals improvements in product visibility and commercial value, as well as the adoption of sustainable and innovative practices by local cooperatives and producers. Among the key findings are the strengthening of local traditions, enhanced product quality, and increased market access despite challenges such as rural exodus. When compared with Embrapa Grape and Wine’s methodology, this study proposes a comprehensive framework for evaluating other GIs. Additionally, the results show that support from SEBRAE and educational institutions through training and technical assistance has positively impacted territorial conditions, contributing to the broader goals of sustainable development. Full article

Many biological markers of normal and disease states can be detected in saliva. The benefits of saliva collection for research include being non-invasive, ease of frequent sample collection, saving time, and being cost-effective. A small volume (≈1 mL) of saliva is enough for these analyses that can be collected in just a few minutes. For “dry” saliva paper matrices, additional drying times (about 30 min) may be needed, but this can be performed at room temperature without the need for freezers and specialized equipment. Together, these make saliva an ideal choice of body fluid for many clinical studies from diagnosis to monitoring measurable biological substances in hospital settings, remote, and other general locations including disaster areas. For these reasons, we have been using saliva (dry as well as wet) from astronauts participating in short- and long-duration space missions for over two decades to conduct viral, stress, and immunological studies. We have also extended the use of saliva to space analogs including bed rest, Antarctica, and closed-chamber studies. Saliva is a biomarker-rich and easily accessible body fluid that could enable larger and faster public health screenings, earlier disease detection, and improved patient outcomes. This review summarizes our lessons learned from utilizing saliva in spaceflight research and highlights the advantages and disadvantages of saliva in clinical diagnostics. Full article

Background: In patients with atrial fibrillation and contraindications for oral anticoagulation, in which an increased risk of stroke remains, a left atrial appendage exclusion should be considered for elimination, because the left atrial appendage is the most common site of thrombus. The aim of this study is to present the first-in-human study results of the Sierra Aegis Left Atrial Appendage Ligation System, a new epicardial-only left atrial appendage closure system. Methods: This study was a prospective, first-in-human, single-center study evaluating the effectiveness and safety of the Sierra Aegis Left Atrial Appendage Ligation System device for epicardial left atrial appendage closure. Seven patients (mean age: 57.3 ± 10.6 years, 71.4% male) were qualified for a left atrial appendage closure because of an increased risk of bleeding with the need for lifelong anticoagulation pharmacology due to an increased risk of stroke. The patients’ preoperative and intraoperative characteristics were collected. Patients were observed during their 1-month, 3-month, 6-month, and 1-year follow-up. Results: The mean procedure time was 21.2 ± 8.2 min. All patients spent 3 days in the hospital including monitoring, the performance of preoperative CT scans, and anatomical evaluation. No tamponade, bleeding, thrombus, or left atrial appendage leakage were observed during the procedure or in-hospital stay. During the 1-month, 3-month, 6-month, and 1-year follow-up visits, none of the patients reported any complications. No tamponade, leakage, or left atrial appendage thrombus were observed. Conclusions: This first-in-human study regarding Sierra use for left atrial appendage closure shows promising results regarding the effectiveness and safety of the Sierra device for use in humans. Full article

Human exploration of the solar system will expose crew members to galactic cosmic radiation (GCR), with a potential for adverse health effects. GCR particles (protons and ions) move at nearly the speed of light and easily penetrate space station walls, as well as the human body. Previously, we have shown reactivation of latent herpesviruses, including herpes simplex virus, Varicella zoster virus, Epstein–Barr virus, and cytomegalovirus (CMV), during stays at the International Space Station. Given the prevalence of latent CMV and the known propensity of space radiation to cause alterations in many cellular processes, we undertook this study to understand the role of GCR in reactivating latent CMV. Latently infected Kasumi cells with CMV were irradiated with ¹³⁷Cs gamma rays, 150 MeV protons, 600 MeV/n carbon ions, 600 MeV/n iron ions, proton ions, and simulated GCR. The CMV copy number increased significantly in the cells exposed to radiation as compared with the non-irradiated controls. Viral genome sequencing did not reveal significant nucleotide differences among the compared groups. However, transcriptome analysis showed the upregulation of transcription of the UL49 ORF, implicating it in the switch from latent to lytic replication. These findings support our hypothesis that GCR may be a strong contributor to the reactivation of CMV infection seen in ISS crew members. Full article

In the context of the Catholic Reformation serious concerns were expressed about the affective potency of naturalistic depictions of beautiful, sensuous figures in religious art. In theological discourse similar anxieties had long been articulated about potential contiguities between elevating, licit desire for an extraordinarily beautiful divinity and base, illicit feeling. In the later fifteenth and early sixteenth centuries, in the decades preceding the Council of Trent, a handful of writers, thinkers and artists asserted a positive connection between spirituality and sexuality. Leonardo da Vinci, and a group of painters working under his aegis in Lombardy, were keenly aware of painting’s capacity to evoke feeling in a viewer. Pictures they produced for domestic devotion featured knowingly sensuous and unusually epicene beauties. This article suggests that this iconography daringly advocated the value of pleasurable sensation to religiosity. Its popularity allows us to envisage beholders who were neither mired in sin, nor seeking to divorce themselves from the physical realm, but engaging afresh with age-old dialectics of body and soul, sexuality and spirituality. Full article

Under the aegis of the National Infectious Diseases Act, the virus transitioned from a category 2 menace to category 5, analogous to seasonal influenza. For this classification to be appropriate, a comprehensive assessment of the immune status of the Japanese population must be conducted to ensure adequacy. The current study has investigated the protracted immune responses of healthcare workers (HCWs) to SARS-CoV-2 in Japan. One year subsequent to the systematically implemented SARS-CoV-2 vaccination campaign among HCWs, humoral and cellular immune responses were sustained at levels as high as or higher than those immediately following the third booster vaccination. Persisting immunity has highlighted the resilience and lasting memory exhibited in HCW defense against the virus, suggesting that the classification of SARS-CoV-2 infection as a category 5 in Japan has appeared judicious. Full article

Over recent decades, the hilly and gully regions of the northern Loess Plateau in Shaanxi province have grappled with severe soil erosion and a precarious ecological milieu. Shaped by urbanization policies, this locale has encountered a gamut of issues, including an imbalance in human–environment dynamics and the degradation of ecological integrity. Consequently, the comprehension of how urban expansion impacts the optimization of regional landscape configurations, the alignment of human–environment interactions in the Loess Plateau’s hilly and gully domains, and the mitigation of urban ecological challenges assumes paramount importance. Leveraging data from land use remote sensing monitoring, alongside inputs from natural geography and socio-economic spheres, and employing methodologies such as landscape pattern indices, we conduct an exhaustive analysis of Zichang City’s urban fabric from 1980 to 2020. Furthermore, employing the CLUE-S model, we undertake multifaceted scenario simulations to forecast urban expansion in Zichang City through to 2035. Our findings delineate two distinct phases in Zichang City’s urban expansion trajectory over the past four decades. From 1980 to 2000, urban construction land in Zichang City experienced a phase of methodical and steady growth, augmenting by 64.98 hectares, alongside a marginal decrease in the landscape shape index (LSI) by 0.02 and a commensurate increase in the aggregation index (AI) by 1.17. Conversely, from 2000 to 2020, urban construction land in Zichang City witnessed an epoch of rapid and haphazard expansion, doubling in expanse, marked by a notable escalation in LSI (2.45) and a corresponding descent in the AI (2.85). The precision of CLUE-S model simulations for Zichang City’s land use alterations registers at 0.88, fulfilling the exigent demand for further urban expansion and land use change prognostication. Under the aegis of the natural development scenario, the augmentation of urban construction land in Zichang City primarily encroaches upon grassland, farmland, and woodland, effectuating an increase of 159.81 hectares. Conversely, under the ambit of urbanization development, urban construction land contends predominantly with farmland, grassland, and woodland, heralding an augmentation of 520.42 hectares. Lastly, under the mantle of ecological protection, urban construction land expansion predominantly encroaches upon grassland, farmland, and woodland, resulting in an augmentation of 4.27 hectares. Through a nuanced analysis of the spatiotemporal evolution of urban expansion and scenario-based simulations, this study endeavors to furnish multi-faceted, scenario-driven, and policy-centric insights for regional planning, urban spatial delineation, and regional ecological safeguarding. Full article

How Nature discovered genetic coding is a largely ignored question, yet the answer is key to explaining the transition from biochemical building blocks to life. Other, related puzzles also fall inside the aegis enclosing the codes themselves. The peptide bond is unstable with respect to hydrolysis. So, it requires some form of chemical free energy to drive it. Amino acid activation and acyl transfer are also slow and must be catalyzed. All living things must thus also convert free energy and synchronize cellular chemistry. Most importantly, functional proteins occupy only small, isolated regions of sequence space. Nature evolved heritable symbolic data processing to seek out and use those sequences. That system has three parts: a memory of how amino acids behave in solution and inside proteins, a set of code keys to access that memory, and a scoring function. The code keys themselves are the genes for cognate pairs of tRNA and aminoacyl-tRNA synthetases, AARSs. The scoring function is the enzymatic specificity constant, k_cat/k_M, which measures both catalysis and specificity. The work described here deepens the evidence for and understanding of an unexpected consequence of ancestral bidirectional coding. Secondary structures occur in approximately the same places within antiparallel alignments of their gene products. However, the polar amino acids that define the molecular surface of one are reflected into core-defining non-polar side chains on the other. Proteins translated from base-paired coding strands fold up inside out. Bidirectional genes thus project an inverted structural duality into the proteome. I review how experimental data root the scoring functions responsible for the origins of coding and catalyzed activation of unfavorable chemical reactions in that duality. Full article

A well-structured digital database is essential for any national priority project as it can provide real-time data analysis and facilitate quick decision making. In recent times, particularly after the COVID-19 pandemic, invasive fungal infections (IFIs) have emerged as a significant public health challenge in India, affecting vulnerable population, including immunocompromised individuals. The lack of comprehensive and well-structured data on IFIs has hindered efforts to understand their true burden and optimize patient care. To address this critical knowledge gap, the ICMR has undertaken a Pan-India pioneer initiative to develop a network of Advanced Mycology Diagnostic research centres in different geographical zones of the country (ICMR-MycoNet). Under the aegis of this project, a clinical registry on IFIs in the ICUs is initiated. This process paper presents a detailed account of the steps involved in the establishment of a web-based data entering and monitoring platform to capture data electronically, ensuring robust and secure data collection and management. This system not only allows participating ICMR-MycoNet centres to enter patient information directly into the database using standardized Case Report Form (CRF) but also includes data validation checks to ensure the accuracy and completeness of entered data. It is complemented by a real-time, web-based, and adaptable data visualization platform. This registry aims to provide crucial epidemiological insights, promote evidence-based hospital infection control programs, and ultimately improve patient outcomes in the face of this formidable healthcare challenge. Full article

This essay documents my three-decade-long journey of connections and resultant transformations between scholarly knowledge and artistic production in my work. In reinvestigating my history with stage and visual arts, I trace the relationship between traditionally ‘alien’ practices and academic understandings of societal and political mass violence and invite the reader to reconsider what academia stands for in order to engage with borderless histories of conflict, violence, and displacement. This essay dwells on how artistic engagement is both a personal and a profoundly political process through which the experience of violence is communicated through thoughts, emotions, hopes, and expressions of trauma. There are also significant ethical concerns present concerning the portrayal of violence, death, and suffering, which the paper discusses under the aegis of ethics of witnessing as responsibility. Full article

The analysis of the 2002 Prestige tanker accident showed how public misinformation can worsen marine pollution incidents, rendering their management suboptimal as these evolve, thus raising the issue of appropriately informing and educating coastal and island populations who are at risk. Two decades later, developments in electronic platforms, including Geographic Information Systems (GIS), the Automatic Identification System (AIS) for ship signal transmission, and social media, provide a set of means for public monitoring of such incidents, creating the possibility to antagonise effectively erroneous or malevolent information, which can hinder efficient actions for containing marine pollution risks even without active training of the populations concerned. The authors, in the framework of the development of the Marine Coastal Observatory and Risk Management project “AEGIS+”, have developed E-S.A.V.E., an online innovative platform that (a) meets the needs of different users as revealed by a survey run across groups of them, (b) uses a suitable Geographic Information System (GIS) environment, (c) cooperates with public authorities, for the reliable update of automated systems, and (d) employs an artificial intelligence (AI)-supported tool for social media monitoring; the platform also provides educational resources and information on national and international resources on marine environmental protection and sustainable maritime logistics. Full article

Since the inception of China’s reform and opening-up policy, the rapidly advancing process of urbanization and the primacy accorded to urban development policies have imparted increasingly profound ramifications on rural domains. Nonetheless, antecedent research has predominantly fixated on urban sprawl, overlooking the spatial metamorphosis of rural settlements and the prospective developmental trajectories within the policy paradigm. Consequently, this inquiry endeavors to scrutinize the evolution of the spatial configuration of rural settlements in She County from the advent of reform and opening-up (1980–2020) utilizing remote sensing data. In tandem, through scenario delineation and the utilization of the CLUE-S model, it aspires to prognosticate the evolving trends in the spatial arrangements of rural settlements in She County by 2035. The empirical findings divulge that (1) The temporal progression of rural settlement spatial configurations in She County over the preceding four decades can be delineated into two discernible phases. From 1980 to 2000, alterations in the number, extent, and spatial morphological attributes of rural settlements remained circumscribed. While the count of rural settlements registered a diminution (by 3), the aggregate extent experienced a marginal augmentation (by 8.45%), concomitant with a gradual gravitation towards regular boundaries, manifesting a stochastic distribution throughout the investigation expanse. Conversely, from 2000 to 2020, the quantity and extent of rural settlements in She County underwent a precipitous augmentation (92 and 36.37%, respectively), characterized by irregular peripheries. (2) The CLUE-S model achieved an overall precision of 0.929, underscoring its applicability in emulating fluctuations in rural settlements. (3) Within the new-type urbanization scenario, the cumulative expanse of rural settlements witnessed a decline of 35.36% compared to the natural development scenario, marked by substantial conversions into grassland and urban land usage. Furthermore, orchestrated planning and directive measures have propelled the consolidation of rural settlements in She County, engendering a more equitable and standardized layout. Under the aegis of the ecological conservation scenario, the total rural settlement area recorded a 0.38% reduction vis-à-vis the natural development scenario, primarily entailing competitive coexistence with arable land, grassland, and urban land usage in spatial terms. Full article