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Search Results (212)

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Keywords = A-68 iceberg

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18 pages, 620 KiB  
Article
The Relationship Between Mood, Competitive Anxiety, and Injuries: A Longitudinal Analysis in High-Performance Female Volleyball Players
by Ana Boladeras, Laura Gil-Caselles, Isabel Moreno-Fernández, Joel Guillén-Cots, Alejo Garcia-Naveira, Roberto Ruiz-Barquín and Aurelio Olmedilla-Zafra
Appl. Sci. 2025, 15(13), 7585; https://doi.org/10.3390/app15137585 - 7 Jul 2025
Viewed by 367
Abstract
Background: Athletic success depends not only on physical performance and psychological well-being but also on the effective emotional regulation of adaptive processes to prevent sports injuries in stressful situations such as competition. This study analyzes the relationship between emotional state, pre-competitive anxiety, and [...] Read more.
Background: Athletic success depends not only on physical performance and psychological well-being but also on the effective emotional regulation of adaptive processes to prevent sports injuries in stressful situations such as competition. This study analyzes the relationship between emotional state, pre-competitive anxiety, and injury incidence in female volleyball players throughout an entire competitive season. Methods: The POMS and CSAI-2 questionnaires were administered before 16 league matches to 21 high-performance players (mean age = 29.66 ± 5.19 years). Results: Players exhibited an iceberg mood profile with low levels of pre-competitive anxiety. Negative moods were higher at the start and decreased over time, while positive moods increased as matches progressed (p < 0.05). Anxiety remained stable throughout. Additionally, 28.6% of players experienced injuries; injured players showed higher cognitive anxiety, lower self-confidence, and emotional instability, suggesting a potential psychological risk profile for injury, even though group differences did not reach statistical significance. Significant correlations were found between Total Mood Disturbance (TMD) and cognitive anxiety (r = 0.49, p < 0.05) and between vigor and self-confidence (r = 0.52, p < 0.01), indicating a bidirectional relationship. A bidirectional relationship between mood and anxiety was confirmed, highlighting the POMS Total Mood Disturbance (TMD) index as a global emotional indicator. Conclusions: These findings suggest that managing worries, self-confidence, and emotional balance are key factors in injury prevention, emphasizing the importance of regularly monitoring and managing psychological indicators as part of injury prevention strategies. Full article
(This article belongs to the Special Issue Applied Sports Performance Analysis)
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27 pages, 2343 KiB  
Article
Leveraging Social Innovation Tools for Advancing Innovative Technologies Towards a Just Energy Transition in Greece
by Paraskevi Giourka, Vasiliki Palla, Ioannis-Athanasios Zornatzis, Komninos Angelakoglou and Georgios Martinopoulos
Energies 2025, 18(13), 3435; https://doi.org/10.3390/en18133435 - 30 Jun 2025
Viewed by 241
Abstract
This study investigates the social and economic dimensions of Greece’s energy transition, focusing on the distinct contexts of mainland Western Macedonia and Insular Greece. Utilizing social innovation tools, including the Stakeholder Persona and the Iceberg Model, the research reveals significant regional variations in [...] Read more.
This study investigates the social and economic dimensions of Greece’s energy transition, focusing on the distinct contexts of mainland Western Macedonia and Insular Greece. Utilizing social innovation tools, including the Stakeholder Persona and the Iceberg Model, the research reveals significant regional variations in stakeholder concerns, priorities, and awareness levels regarding energy transition issues. Findings indicate that while Insular Greece prioritizes energy security and public acceptance of renewable energy solutions, mainland Greece emphasizes job security and economic diversification. The study highlights the necessity for tailored energy transition strategies that address local needs and foster community engagement. Furthermore, it underscores the importance of enhancing awareness and understanding of methodologies such as Life Cycle Assessment and Life Cycle Cost Analysis to empower stakeholders in making informed decisions. Integrating insights from various layers of the Iceberg Model, this research provides a framework for developing innovative technologies and policies that support a fair and sustainable energy transition in Greece, ensuring that no community is left behind in the global shift towards sustainability. This comprehensive approach seeks to mitigate environmental impacts but also to create economic opportunities that align with each community’s social and cultural fabric. Full article
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16 pages, 4568 KiB  
Article
Study of Numerical Modeling Method for Precooling of Spherical Horticultural Produce Stacked Symmetrically in Vented Package
by Xifang Wang, Zhongyang Fan, Chuanhui Zhu and Hongbin Liu
Symmetry 2025, 17(6), 810; https://doi.org/10.3390/sym17060810 - 22 May 2025
Viewed by 308
Abstract
Numerical simulation has become a pivotal tool for analyzing airflow dynamics and temperature patterns during the precooling of postharvest horticultural products stacked in vented package. In this study, a three-dimensional mathematical model for iceberg lettuces stacked symmetrically in plastic crate was developed. The [...] Read more.
Numerical simulation has become a pivotal tool for analyzing airflow dynamics and temperature patterns during the precooling of postharvest horticultural products stacked in vented package. In this study, a three-dimensional mathematical model for iceberg lettuces stacked symmetrically in plastic crate was developed. The influence of the physical model at different gap sizes on the simulation accuracy was studied by assessing wall drag coefficient, airflow distribution, and heat transfer efficiency. The results show a reasonable decrease in the drag coefficient with an increasing gap size to 6 mm in terms of airflow distribution inside the plastic crate; any further increase in gap size and the average airflow velocity in both windward and leeward sides decreases rapidly. Interestingly, the gap size exhibited a limited impact on heat transfer characteristics during the cooling process. Thus, 6 mm was found to be the optimal distance to ensure good accuracy in simulation results and reduce the complexity of grid division. The numerical model was verified by experimental data. Moreover, the validation confirms good consistency between the simulated predictions and experimental measurements. This study provides a theoretical basis for establishing a reliable numerical model for the precooling of agricultural produce stacked symmetrically in a vented package. Full article
(This article belongs to the Section Engineering and Materials)
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16 pages, 1815 KiB  
Article
Cultural Representation in Chinese University English Language Teaching Textbooks
by Hui Zhang and Nur Rasyidah Mohd Nordin
Educ. Sci. 2025, 15(5), 601; https://doi.org/10.3390/educsci15050601 - 13 May 2025
Viewed by 996
Abstract
Globalization enhances communication among people from different linguistic and cultural backgrounds. As cultural artifacts, English language teaching textbooks are crucial media for learners’ intercultural education, especially under the concept of English as a lingua franca. This study investigated the cultural elements in Chinese [...] Read more.
Globalization enhances communication among people from different linguistic and cultural backgrounds. As cultural artifacts, English language teaching textbooks are crucial media for learners’ intercultural education, especially under the concept of English as a lingua franca. This study investigated the cultural elements in Chinese university English textbooks via the approach of content analysis. Specifically, a set of Chinese English language teaching textbooks was selected as the sample and the audio and video materials were transcribed into texts as the data. The results were displayed by the counts of cultural elements. The data revealed that international cultures and source cultures were highlighted, and the textbooks were not oriented toward native-speakers’ English. Moreover, the distribution of cultural categories across the textbooks remains imbalanced, with cultural products occupying the largest proportion, and cultural perspectives displayed the least. This is mainly attributed to the fact that cultural perspectives are implicit cultures under the surface of the iceberg, and notably, they are sporadically reflected by the cultural products, practices, and persons. The findings provide suggestions that writers include balanced cultural elements in compiling English language teaching textbooks, and teachers scrutinize cultural representation and design intercultural activities. Full article
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21 pages, 1711 KiB  
Review
Proposal for a Green Business Model for Biofortified Foods in the Municipality of Chocontá, Cundinamarca
by Angie Tatiana Ortega-Ramírez, Luisa Fernanda Escobar Toro and Miriam Reyes Tovar
Agriculture 2025, 15(9), 991; https://doi.org/10.3390/agriculture15090991 - 2 May 2025
Viewed by 482
Abstract
Historically, agriculture has been a key driver of rural development. Therefore, outlining strategies that enhance agricultural production for economic sustenance, quality of life, and the durability of natural resources puts us on the right path to ensure sustainability. This is the focus of [...] Read more.
Historically, agriculture has been a key driver of rural development. Therefore, outlining strategies that enhance agricultural production for economic sustenance, quality of life, and the durability of natural resources puts us on the right path to ensure sustainability. This is the focus of the green business model proposal, which aims to provide farmers with tools to strengthen their daily activities while preserving the ecosystem, ensuring that future generations can enjoy its benefits. Opening a marketing channel under green business guidelines for iceberg lettuce as a biofortified food crop in the municipality of Chocontá in Cundinamarca, Colombia, is an innovative approach to addressing food security issues. Currently, 24.8% of households in 23 cities consume two meals a day or fewer. This proposal also seeks to influence crop rotation in the municipality, helping to mitigate soil degradation in the area. Full article
(This article belongs to the Special Issue Sustainability and Energy Economics in Agriculture—2nd Edition)
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18 pages, 525 KiB  
Article
The Overlap of Allergic Disorders and Upper Gastrointestinal Symptoms: Beyond Eosinophilic Esophagitis
by Oksana Wojas, Edyta Krzych-Fałta, Paulina Żybul, Marta Żalikowska-Gardocka, Tomasz Ilczuk, Konrad Furmańczyk, Bolesław Samoliński and Adam Przybyłkowski
Nutrients 2025, 17(8), 1355; https://doi.org/10.3390/nu17081355 - 16 Apr 2025
Viewed by 1348
Abstract
Eosinophilic esophagitis (EoE) is a chronic disease which clinically presents with symptoms related to esophageal dysfunction, while pathologically it is characterized by eosinophilic infiltration of esophageal epithelium. Most patients with EoE present with food and/or inhalant allergy symptoms. The results of animal model [...] Read more.
Eosinophilic esophagitis (EoE) is a chronic disease which clinically presents with symptoms related to esophageal dysfunction, while pathologically it is characterized by eosinophilic infiltration of esophageal epithelium. Most patients with EoE present with food and/or inhalant allergy symptoms. The results of animal model studies and genetic studies, as well as the efficacy of elimination diets in managing the symptoms, suggest an atopic background of the disease. The aim of this study was to evaluate the prevalence of EoE in a group of patients with upper gastrointestinal symptoms and food and/or inhalant allergies and to assess the influence of drugs used in type I allergies on the results of endoscopic, histopathological, and immunohistochemical tests. Methods: This was a prospective observational study. Patients with inhalant/food allergies and upper esophageal symptoms constituted the study group while patients without allergies who were diagnosed with dyspepsia or irritable bowel syndrome constituted the control group. All study group subjects underwent allergy testing, including prick testing and blood tests. All participants underwent a gastroscopy with specimen collection. Esophageal specimens were stained for eotaxin-1 and desmoglein-1. Results: Based on histopathology results, eosinophilic esophagitis was found in 9 of the 73 patients from the study group. All patients with EoE presented with multimorbidity and were diagnosed with at least one allergic disease in addition to EoE. Positive staining for CCL-11 was found in 56 (78%) patients in the study group, including all patients with EoE while only 3 (17%) individuals from the control group showed positive staining. The presence of DSG-1 in esophageal specimens was detected in 6 (7%) subjects from the study group in contrast to 14 (78%) subjects from the control group. DSG-1 was not found in any of the specimens of patients diagnosed with EoE. Conclusions: EoE is a rare disease, usually accompanied by allergic multimorbidity. Positive staining for eotaxin-1 and negative staining for desmoglein-1 in patients with esophageal symptoms and allergies but who did not meet EoE diagnostic criteria could be indicative of subclinical course of the disease or a masking effect of corticosteroids. It is now vitally important for both researchers and practicing clinicians to recognize that eosinophilic esophagitis (EoE) is not a homogeneous disease but rather consists of multiple subtypes (phenotypes). The so-called “classic” form of EoE—defined by current diagnostic criteria as the presence of more than 15 eosinophils per high power field on histopathological examination—appears to represent only the tip of the iceberg. There is an urgent need for further research in order to refine endoscopic techniques, expand the scope of histopathological assessments, and identify novel biomarkers to better define the distinct phenotypes of eosinophilic esophagitis. Full article
(This article belongs to the Section Nutritional Immunology)
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16 pages, 1024 KiB  
Article
Global Archaeal Diversity Revealed Through Massive Data Integration: Uncovering Just Tip of Iceberg
by Antonios Kioukis, Antonio Pedro Camargo, Pavlos Pavlidis, Ioannis Iliopoulos, Nikos C Kyrpides and Ilias Lagkouvardos
Microorganisms 2025, 13(3), 598; https://doi.org/10.3390/microorganisms13030598 - 5 Mar 2025
Viewed by 1100
Abstract
The domain of Archaea has gathered significant interest for its ecological and biotechnological potential and its role in helping us to understand the evolutionary history of Eukaryotes. In comparison to the bacterial domain, the number of adequately described members in Archaea is [...] Read more.
The domain of Archaea has gathered significant interest for its ecological and biotechnological potential and its role in helping us to understand the evolutionary history of Eukaryotes. In comparison to the bacterial domain, the number of adequately described members in Archaea is relatively low, with less than 1000 species described. It is not clear whether this is solely due to the cultivation difficulty of its members or, indeed, the domain is characterized by evolutionary constraints that keep the number of species relatively low. Based on molecular evidence that bypasses the difficulties of formal cultivation and characterization, several novel clades have been proposed, enabling insights into their metabolism and physiology. Given the extent of global sampling and sequencing efforts, it is now possible and meaningful to question the magnitude of global archaeal diversity based on molecular evidence. To do so, we extracted all sequences classified as Archaea from 500 thousand amplicon samples available in public repositories. After processing through our highly conservative pipeline, we named this comprehensive resource the ‘Global Archaea Diversity’ (GAD), which encompassed nearly 3 million molecular species clusters at 97% similarity, and organized it into over 500 thousand genera and nearly 100 thousand families. Saline environments have contributed the most to the novel taxa of this previously unseen diversity. The majority of those 16S rRNA gene sequence fragments were verified by matches in metagenomic datasets from IMG/M. These findings reveal a vast and previously overlooked diversity within the Archaea, offering insights into their ecological roles and evolutionary importance while establishing a foundation for the future study and characterization of this intriguing domain of life. Full article
(This article belongs to the Special Issue Earth Systems: Shaped by Microbial Life)
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11 pages, 1209 KiB  
Article
Association of Escherichia coli O157:H7 Density Change with Hydrogen Peroxide but Not Carbohydrate Concentration in the Leaf Content of Different Lettuce Types and Spinach
by Maria T. Brandl, Sui S. T. Hua and Siov B. L. Sarreal
Foods 2025, 14(4), 709; https://doi.org/10.3390/foods14040709 - 19 Feb 2025
Cited by 1 | Viewed by 623
Abstract
Leafy greens injuries occur from farm to table, causing leakage of cellular contents that promote the multiplication of foodborne pathogens and impose oxidative stress. Fresh beverages made from blended uncooked fruit and vegetables have become a popular food. The effect of cellular contents [...] Read more.
Leafy greens injuries occur from farm to table, causing leakage of cellular contents that promote the multiplication of foodborne pathogens and impose oxidative stress. Fresh beverages made from blended uncooked fruit and vegetables have become a popular food. The effect of cellular contents of different leafy greens on the multiplication of the important pathogen Escherichia coli O157:H7 (EcO157) under temperature abuse was investigated. Leafy greens consisted of spinach and different lettuce types (romaine, iceberg, butterhead, green leaf, and red leaf). Fructose, glucose, and sucrose concentrations in the leaves were quantified by HPLC. H2O2 concentration was measured via a peroxidase-based assay. Young leaves of iceberg, romaine, and green leaf lettuce held significantly greater total amounts of the three carbohydrates than middle-aged leaves. Except for iceberg and red leaf lettuce, all middle-aged leaves contained greater H2O2 than young leaves. EcO157 density change in leaf contents over 5 h incubation related neither to individual nor total carbohydrate concentration but was negatively associated with H2O2 concentration (regression analysis; p < 0.05). Given the common use of antioxidants to maintain the organoleptic aspects of homogenized produce beverages and certain fresh-cut produce, the antimicrobial effect of reactive oxygen species may be important to preserve in ensuring their microbial safety. Full article
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18 pages, 6889 KiB  
Article
Machine Learning-Based Detection of Icebergs in Sea Ice and Open Water Using SAR Imagery
by Zahra Jafari, Pradeep Bobby, Ebrahim Karami and Rocky Taylor
Remote Sens. 2025, 17(4), 702; https://doi.org/10.3390/rs17040702 - 19 Feb 2025
Cited by 1 | Viewed by 1066
Abstract
Icebergs pose significant risks to shipping, offshore oil exploration, and underwater pipelines. Detecting and monitoring icebergs in the North Atlantic Ocean, where darkness and cloud cover are frequent, is particularly challenging. Synthetic aperture radar (SAR) serves as a powerful tool to overcome these [...] Read more.
Icebergs pose significant risks to shipping, offshore oil exploration, and underwater pipelines. Detecting and monitoring icebergs in the North Atlantic Ocean, where darkness and cloud cover are frequent, is particularly challenging. Synthetic aperture radar (SAR) serves as a powerful tool to overcome these difficulties. In this paper, we propose a method for automatically detecting and classifying icebergs in various sea conditions using C-band dual-polarimetric images from the RADARSAT Constellation Mission (RCM) collected throughout 2022 and 2023 across different seasons from the east coast of Canada. This method classifies SAR imagery into four distinct classes: open water (OW), which represents areas of water free of icebergs; open water with target (OWT), where icebergs are present within open water; sea ice (SI), consisting of ice-covered regions without any icebergs; and sea ice with target (SIT), where icebergs are embedded within sea ice. Our approach integrates statistical features capturing subtle patterns in RCM imagery with high-dimensional features extracted using a pre-trained Vision Transformer (ViT), further augmented by climate parameters. These features are classified using XGBoost to achieve precise differentiation between these classes. The proposed method achieves a low false positive rate of 1% for each class and a missed detection rate ranging from 0.02% for OWT to 0.04% for SI and SIT, along with an overall accuracy of 96.5% and an area under curve (AUC) value close to 1. Additionally, when the classes were merged for target detection (combining SI with OW and SIT with OWT), the model demonstrated an even higher accuracy of 98.9%. These results highlight the robustness and reliability of our method for large-scale iceberg detection along the east coast of Canada. Full article
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20 pages, 2315 KiB  
Article
Fulvic Acid, Amino Acids, and Vermicompost Enhanced Yield and Improved Nutrient Profile of Soilless Iceberg Lettuce
by Beyza Keskin, Yelderem Akhoundnejad, Hayriye Yildiz Dasgan and Nazim S. Gruda
Plants 2025, 14(4), 609; https://doi.org/10.3390/plants14040609 - 18 Feb 2025
Cited by 1 | Viewed by 1654
Abstract
Soilless cultivation systems are sustainable innovations in modern agriculture, promoting high efficiency per unit area, supporting food sustainability, and addressing the growing demand for high-quality produce with minimal environmental impact. This study evaluates the effects of fulvic acid, amino acid, and vermicompost biostimulants [...] Read more.
Soilless cultivation systems are sustainable innovations in modern agriculture, promoting high efficiency per unit area, supporting food sustainability, and addressing the growing demand for high-quality produce with minimal environmental impact. This study evaluates the effects of fulvic acid, amino acid, and vermicompost biostimulants on the growth, yield, and nutrient profile of soilless-grown iceberg lettuce (Lactuca sativa var. capitata) in floating culture under controlled glasshouse conditions. Two experiments were conducted to determine the most effective concentrations and combinations of biostimulants. In the first experiment, varying doses of fulvic acid (40 and 80 ppm), amino acid (75 and 100 ppm), and vermicompost (1 and 2 mL L−1) were tested alongside a control. Optimal doses were identified based on their positive effects on lettuce growth and yield. The second experiment examined combinations of fulvic acid, amino acid, and vermicompost extract compared to a control. Biostimulants improved lettuce growth, nutrient uptake, and antioxidants. Vermicompost boosted root biomass and leaf area, while fulvic acid and amino acid reduced nitrates and increased dry matter. Fulvic acid and vermicompost resulted in the highest yield (17.15 kg/m2, 18.2% increase), and the combined treatment maximized antioxidants, increasing vitamin C by 17.16%, total phenols by 52.54%, and flavonoids by 52.38%. These findings highlight the potential of biostimulants as eco-friendly solutions for optimizing lettuce production in soilless systems. Full article
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26 pages, 7065 KiB  
Article
Water Surface Temperature Dynamics of the Three Largest Ice-Contact Lakes in the Patagonia Icefield over the Last 20 Years
by Shaochun Zhao, Hongyan Sun, Jie Cheng and Guoqing Zhang
Water 2025, 17(3), 385; https://doi.org/10.3390/w17030385 - 30 Jan 2025
Viewed by 943
Abstract
The Patagonia Icefield, the largest ice mass in the Southern Hemisphere outside Antarctica, has experienced significant growth and expansion of ice-contact lakes in recent decades, with lake surface water temperature (LSWT) being one of the key influencing factors. LSWT affects glacier melting at [...] Read more.
The Patagonia Icefield, the largest ice mass in the Southern Hemisphere outside Antarctica, has experienced significant growth and expansion of ice-contact lakes in recent decades, with lake surface water temperature (LSWT) being one of the key influencing factors. LSWT affects glacier melting at the waterline and accelerates glacier mass loss. However, the observations of ice-contact LSWT are often limited to short-term, site-based field measurements, which hinders long-term, whole-lake monitoring. This study examines LSWT for the three largest ice-contact lakes in the Patagonia Icefield—Lake Argentino, Lake Viedma, and Lake O’Higgins, each exceeding 1000 km2—and the three largest nearby non-ice-contact lakes for comparison using MODIS data between 2002 and 2022. In 2022, the mean LSWTs for Lake Argentino, Lake Viedma, and Lake O’Higgins were 7.2, 7.0, and 6.4 °C, respectively. In summer, ice-contact lakes exhibited wider LSWT ranges and more pronounced cooling near glacier termini and warming farther away compared to other seasons, demonstrating glacier melt cooling and its seasonal variability. Over the past 20 years, both Lake Viedma and Lake O’Higgins showed a warming rate of +0.20 °C dec−1, p > 0.1, with slower warming near the glacier, reflecting glacier contact suppression on the LSWT trend. Conversely, Lake Argentino displayed a significant warming rate of +0.43 °C dec−1 (p < 0.05), with faster rates near the glacier terminus, possibly linked to a prolonged and large (>64 km2) iceberg accumulation event from March 2010 to October 2011 in Glacier Upsala’s fjord. Iceberg mapping shows that larger events caused more pronounced short-term (24 days) LSWT cooling in Lake Argentino’s ice-proximal region. This study highlights the role of glacier–lake interactions including calving events in regulating ice-contact lake water temperature. Full article
(This article belongs to the Section Hydrology)
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8 pages, 1690 KiB  
Proceeding Paper
Physicochemical and Functional Value of Lettuce: Effect of Mulching Technique Used During Production on Postharvest Storage
by Roxana E. González, Melisa Lanza Volpe and Verónica C. Soto Vargas
Biol. Life Sci. Forum 2024, 40(1), 12; https://doi.org/10.3390/blsf2024040012 - 20 Jan 2025
Viewed by 712
Abstract
Lettuce is one of the most important vegetable crops cultivated worldwide. Mulch or mulching is used to protect the soil surface, create a physical barrier, and provide a more suitable environment for crops. The aim of our work was to evaluate the effect [...] Read more.
Lettuce is one of the most important vegetable crops cultivated worldwide. Mulch or mulching is used to protect the soil surface, create a physical barrier, and provide a more suitable environment for crops. The aim of our work was to evaluate the effect of different mulching techniques and test their effect on the morpho-physiological performance and nutritive value of Iceberg lettuce plants, and their changes during postharvest storage. Mulching soil treatments had a significant impact on biometric measurements such as yield, physicochemical values, and functional value in lettuce heads. Organic mulch had the best result, improving the bioactive compounds in lettuce. Full article
(This article belongs to the Proceedings of The 5th International Electronic Conference on Foods)
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17 pages, 3522 KiB  
Article
A Formal Fuzzy Concept-Based Approach for Association Rule Discovery with Optimized Time and Storage
by Gamal F. Elhady, Haitham Elwahsh, Maazen Alsabaan, Mohamed I. Ibrahem and Ebtesam Shemis
Mathematics 2024, 12(22), 3590; https://doi.org/10.3390/math12223590 - 16 Nov 2024
Cited by 1 | Viewed by 1183
Abstract
Association Rule Mining (ARM) relies on concept lattices as an effective knowledge representation structure. However, classical ARM methods face significant limitations, including the generation of misleading rules during data-to-formal-context mapping and poor handling of heterogeneous data types such as linguistic, continuous, and imprecise [...] Read more.
Association Rule Mining (ARM) relies on concept lattices as an effective knowledge representation structure. However, classical ARM methods face significant limitations, including the generation of misleading rules during data-to-formal-context mapping and poor handling of heterogeneous data types such as linguistic, continuous, and imprecise data. This study aims to address these limitations by introducing a novel fuzzy data structure called the “fuzzy iceberg lattice” and its corresponding construction algorithm. The primary objectives of this study are to enhance the efficiency of extracting and visualizing frequent fuzzy closed item sets and to optimize both execution time and storage requirements. The necessity of this research stems from the high computational cost and redundancy associated with traditional fuzzy approaches, which, while capable of managing quantitative and imprecise data, are often impractical for large-scale applications in real scenarios. The proposed approach incorporates a ‘fuzzy min-max basis algorithm’ to derive exact and approximate rule bases from the extracted fuzzy closed item sets, eliminating redundancy while preserving valuable insights. Experimental results on benchmark datasets demonstrate that the proposed fuzzy iceberg lattice outperforms traditional fuzzy concept lattices, achieving an average reduction of 74.75% in execution time and 70.53% in memory usage. This efficiency gain, coupled with the lattice’s ability to handle crisp, quantitative, fuzzy, and heterogeneous data types, underscores its potential to advance ARM by yielding a manageable number of high-quality fuzzy concepts and rules. Full article
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13 pages, 851 KiB  
Article
Natural Inhibitors of the Polyphenol Oxidase Activity Isolated from Shredded Stored Iceberg Lettuce (Lactuca sativa L.)
by Małgorzata Sierocka and Michał Świeca
Appl. Sci. 2024, 14(21), 9980; https://doi.org/10.3390/app14219980 - 31 Oct 2024
Cited by 1 | Viewed by 2716
Abstract
Polyphenol oxidase (PPO) is the key enzyme responsible for enzymatic browning. To extend the shelf life of shredded lettuce, knowledge about biochemical PPO properties is required. The characterization of the enzyme from shredded, cold-stored lettuce was performed using pyrocatechol and the endogenous substrate [...] Read more.
Polyphenol oxidase (PPO) is the key enzyme responsible for enzymatic browning. To extend the shelf life of shredded lettuce, knowledge about biochemical PPO properties is required. The characterization of the enzyme from shredded, cold-stored lettuce was performed using pyrocatechol and the endogenous substrate (ES) (lettuce phenolics). The optimum pH and temperature for PPO activity were 5 and 50 °C, respectively. Natural infusions used as the PPO inhibitors (IC50) were ranked as follows: lovage (0.09%), marjoram (0.13%), orange peel (0.14%), oregano (0.15%), basil (0.22%), lemon peel (0.24%), parsley leaves (0.58%), and wheat bran (1.06%). Among well-recognized PPO inhibitors, kojic acid (0.00043%), ascorbic acid (0.00053%), and L-cysteine (0.00085%) were the most effective. Among the metal ions, MgCl2, FeCl2, and CaCl2 at 0.5 mM inhibited the PPO activity most effectively (by 28%, 27%, and 21%, respectively). The substrate used (pyrocatechol/ES) significantly influenced the enzyme inhibition. Using pyrocatechol, the lovage extract acted in a mixed mode (Kmi = 27.8 mM, Vmaxi = 2.03 mU), while the ES acted according to the non-competitive mode (Kmi= 0.57 mg GAE/mL, Vmax = 0.0046 U). The study confirms that natural extracts are more effective than L-cysteine when the ES is used. A pre-storage treatment with an infusion may be potentially used to improve the quality of shredded lettuce. Full article
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20 pages, 5447 KiB  
Article
Plastic Polymers and Antibiotic Resistance in an Antarctic Environment (Ross Sea): Are We Revealing the Tip of an Iceberg?
by Gabriella Caruso, Maurizio Azzaro, Ombretta Dell’Acqua, Maria Papale, Angelina Lo Giudice and Pasqualina Laganà
Microorganisms 2024, 12(10), 2083; https://doi.org/10.3390/microorganisms12102083 - 17 Oct 2024
Cited by 3 | Viewed by 1522
Abstract
Microbial colonization of plastic polymers in Antarctic environments is an under-investigated issue. While several studies are documenting the spread of plastic pollution in the Ross Sea, whether the formation of a plastisphere (namely the complex microbial assemblage colonizing plastics) may favor the spread [...] Read more.
Microbial colonization of plastic polymers in Antarctic environments is an under-investigated issue. While several studies are documenting the spread of plastic pollution in the Ross Sea, whether the formation of a plastisphere (namely the complex microbial assemblage colonizing plastics) may favor the spread of antibiotic-resistant bacteria (ARB) in this marine environment is unknown yet. A colonization experiment was performed in this ecosystem, aiming at exploring the potential role of plastic polymers as a reservoir of antibiotic resistance. To this end, the biofilm-producing activity and the antibiotic susceptibility profiles of bacterial strains isolated from biofilms colonizing submerged polyvinylchloride and polyethylene panels were screened. The colonization experiment was carried out at two different sites of the Ross Sea, namely Road Bay and Tethys Bay. Most of bacterial isolates were able to produce biofilm; several multidrug resistances were detected in the bacterial members of biofilms associated to PVC and PE (also named as the plastisphere), as well as in the bacterial strains isolated from the surrounding water. The lowest percentage of ARB was found in the PE-associated plastisphere from the not-impacted (control) Punta Stocchino station, whereas the highest one was detected in the PVC-associated plastisphere from the Tethys Bay station. However, no selective enrichment of ARB in relation to the study sites or to either type of plastic material was observed, suggesting that resistance to antibiotics was a generalized widespread phenomenon. Resistance against to all the three classes of antibiotics assayed in this study (i.e., cell wall antibiotics, nucleic acids, and protein synthesis inhibitors) was observed. The high percentage of bacterial isolates showing resistance in remote environments like Antarctic ones, suffering increasing anthropic pressure, points out an emerging threat with a potential pathogenic risk that needs further deepening studies. Full article
(This article belongs to the Special Issue Microbial Colonization in Marine Environments)
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