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17 pages, 1097 KiB  
Review
Natural Feed Additives in Sub-Saharan Africa: A Systematic Review of Efficiency and Sustainability in Ruminant Production
by Zonaxolo Ntsongota, Olusegun Oyebade Ikusika and Thando Conference Mpendulo
Ruminants 2025, 5(3), 36; https://doi.org/10.3390/ruminants5030036 - 6 Aug 2025
Abstract
Ruminant livestock production plays a crucial role in the agricultural systems of Sub-Saharan Africa, significantly supporting rural livelihoods through income generation, improved nutrition, and employment opportunities. Despite its importance, the sector continues to face substantial challenges, such as low feed quality, seasonal feed [...] Read more.
Ruminant livestock production plays a crucial role in the agricultural systems of Sub-Saharan Africa, significantly supporting rural livelihoods through income generation, improved nutrition, and employment opportunities. Despite its importance, the sector continues to face substantial challenges, such as low feed quality, seasonal feed shortages, and climate-related stresses, all of which limit productivity and sustainability. Considering these challenges, the adoption of natural feed additives has emerged as a promising strategy to enhance animal performance, optimise nutrient utilisation, and mitigate environmental impacts, including the reduction of enteric methane emissions. This review underscores the significant potential of natural feed additives such as plant extracts, essential oils, probiotics, and mineral-based supplements such as fossil shell flour as sustainable alternatives to conventional growth promoters in ruminant production systems across the region. All available documented evidence on the topic from 2000 to 2024 was collated and synthesised through standardised methods of systematic review protocol—PRISMA. Out of 319 research papers downloaded, six were included and analysed directly or indirectly in this study. The results show that the addition of feed additives to ruminant diets in all the studies reviewed significantly (p < 0.05) improved growth parameters such as average daily growth (ADG), feed intake, and feed conversion ratio (FCR) compared to the control group. However, no significant (p > 0.05) effect was found on cold carcass weight (CCW), meat percentage, fat percentage, bone percentage, or intramuscular fat (IMF%) compared to the control. The available evidence indicates that these additives can provide tangible benefits, including improved growth performance, better feed efficiency, enhanced immune responses, and superior meat quality, while also supporting environmental sustainability by reducing nitrogen excretion and decreasing dependence on antimicrobial agents. Full article
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15 pages, 744 KiB  
Article
Investigation of Effects of Low Ruminal pH Values on Serum Concentrations of Macrominerals, Trace Elements, and Vitamins and Oxidative Status of Dairy Cows
by Panagiotis D. Katsoulos, Bengü Bilgiç, Duygu Tarhan, Fatma Ateş, Suat Ekin, Süleyman Kozat, Banu Dokuzeylül, Mehmet Erman Or, Emmanouil Kalaitzakis, Georgios E. Valergakis and Nikolaos Panousis
Ruminants 2025, 5(3), 35; https://doi.org/10.3390/ruminants5030035 - 2 Aug 2025
Viewed by 298
Abstract
Due to the feeding system (high-concentrate diet) during the early lactation stage, ruminal pH in dairy cows follows a diurnal pattern and can remain below the critical level of 5.5 for extended periods of the day. This study aimed to evaluate the effect [...] Read more.
Due to the feeding system (high-concentrate diet) during the early lactation stage, ruminal pH in dairy cows follows a diurnal pattern and can remain below the critical level of 5.5 for extended periods of the day. This study aimed to evaluate the effect of low ruminal pH on blood concentrations of certain macrominerals, trace minerals, and fat-soluble vitamins and on the oxidative status of dairy cows during the first half of lactation. Fifty-three randomly selected lactating Holstein cows were used; blood and ruminal fluid samples were collected from all cows on days 30, 90 and 150 of lactation. Blood samples were obtained via coccygeal venipuncture, while the ruminal fluid was obtained by rumenocentesis and the pH was measured immediately after collection. Using a threshold pH of 5.5, samples were classified as normal (pH > 5.5) or low pH (pH ≤ 5.5). Serum concentrations of Ca, Mg, K, Cr, Mn, Zn, Se, and vitamins A, D3, E, and K were not significantly affected by ruminal pH, either by days in milk or by their interaction (p > 0.05). Plasma malondialdehyde and reduced glutathione followed the same trend (p > 0.05). Copper concentration was significantly higher (p < 0.05), and Fe concentration tended to be higher in cows with low pH compared to those with normal pH (p = 0.052). On day 150 of lactation, Cu, Fe, and Co concentrations were significantly higher in low-pH cows compared to normal-pH cows (p < 0.05). Low ruminal pH is associated with significant changes in serum concentrations of copper, iron, and cobalt but has no significant effect on the oxidative status of the animals or on the serum concentrations of the macro elements and fat-soluble vitamins studied. Full article
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11 pages, 270 KiB  
Article
Comparison of Contemporary Grazing Cattle and Bison Greenhouse Gas Emissions in the Southern Great Plains
by Maria De Bernardi, Carlee M. Salisbury, Haley E. Larson, Matthew R. Beck and Logan R. Thompson
Ruminants 2025, 5(3), 34; https://doi.org/10.3390/ruminants5030034 - 28 Jul 2025
Viewed by 304
Abstract
The objective of this analysis was to compare the greenhouse gas (GHG) emissions from contemporary grazing cattle production with bison grazing, both modern and historical. The data sets used in this analysis were derived from existing research and conservation properties located outside of [...] Read more.
The objective of this analysis was to compare the greenhouse gas (GHG) emissions from contemporary grazing cattle production with bison grazing, both modern and historical. The data sets used in this analysis were derived from existing research and conservation properties located outside of Manhattan, KS (USA), which are home to stocker cattle, cow–calf production (CCS), and grazing bison. For stocker cattle, 10 years of animal production data (2007–2016) from season-long stocking (SLS, grazing 156 d) and intensive early stocking systems (IES; 76 grazing d and 2× stocking density) were used for GHG calculations. Enteric CH4, manure CH4, and direct nitrous oxide emissions were estimated using the IPCC tier 2 methodology. Historic bison (HGB) enteric CH4 estimates were calculated using a stocking density of 0.15 ha/animal and assuming that only 13% of grassland was used by bison each year. Within contemporary systems, IES had the lowest emissions (463.3 kg CO2-eq./ha/yr), while SLS, CCS, and MGB had the highest estimates (494.7, 493.9, and 595.9 kg CO2-eq./ha/yr, respectively). HGB had the lowest estimated annual emissions at 295.7 kg CO2-eq./ha/yr. These results imply that the historic grazing baseline of this grassland system is lower but similar to that of contemporary grazing cattle in the Great Plains region. Full article
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11 pages, 1796 KiB  
Article
Head Sexual Characterization of Sanmartinero Creole Bovine Breed Assessed by Geometric Morphometric Methods
by Arcesio Salamanca-Carreño, Pere M. Parés-Casanova, Mauricio Vélez-Terranova, David E. Rangel-Pachón, Germán Martínez-Correal and Jaime Rosero-Alpala
Ruminants 2025, 5(3), 33; https://doi.org/10.3390/ruminants5030033 - 21 Jul 2025
Viewed by 380
Abstract
Geometric morphometrics is performed on different species in different contexts. Here, the aim was to investigate morphological differences in the head of the Sanmartinero Creole bovine to examine head shape variations between sexes using geometric morphometric methods. A sample of cranial pictures of [...] Read more.
Geometric morphometrics is performed on different species in different contexts. Here, the aim was to investigate morphological differences in the head of the Sanmartinero Creole bovine to examine head shape variations between sexes using geometric morphometric methods. A sample of cranial pictures of 43 animals (13 males and 30 females) was obtained, and form (size + shape) was studied by means of geometric morphometric techniques using a set of 14 landmarks. This approach eliminated potential dietary effects, ensuring that the observed shape variations were primarily due to intrinsic morphological differences. Sexual dimorphism was found in form (for both size and shape) of the head of the Sanmartinero Creole bovine breed. Males had significantly larger heads based on centroid size (U = 714, p = 0.0004), confirming true sexual size differences, and Principal Component Analysis revealed overlapping head shapes with sexual dimorphism concentrated at midline sagittal landmarks (between the most rostral and caudal orbit points) and paired lateral points, indicating that males have broader and longer heads. The two evaluated characters (head size and shape) are of special interest for the conservation of the breed, especially in those cases whose objectives are to maintain the uniqueness, distinctiveness, and uniformity of the populations. This study analyzed animals subjected to the same feeding program, ensuring the elimination of additional variables. Full article
(This article belongs to the Special Issue Feature Papers of Ruminants 2024–2025)
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22 pages, 929 KiB  
Article
Effects of Salsola tragus as a Forage Source During Fattening on Productivity and Meat Metabolomics of Rambouillet Lambs
by José Alejandro Roque-Jiménez, Lorena Diaz de León-Martinez, German David Mendoza-Martínez, Rogelio Flores-Ramírez, Guillermo Espinosa-Reyes, Alejandro E. Relling, Ulises Macias-Cruz, Marisol López-Romero and Héctor Aarón Lee-Rangel
Ruminants 2025, 5(3), 32; https://doi.org/10.3390/ruminants5030032 - 16 Jul 2025
Viewed by 254
Abstract
The aims of the current study were to characterize the natural compounds of Salsola tragus via GC-MS and determine its effects as a forage source on lamb growth, in vitro rumen fermentation kinetics, carcass quality, cellular damage, and metabolomic meat interaction. Twenty-one Rambouillet [...] Read more.
The aims of the current study were to characterize the natural compounds of Salsola tragus via GC-MS and determine its effects as a forage source on lamb growth, in vitro rumen fermentation kinetics, carcass quality, cellular damage, and metabolomic meat interaction. Twenty-one Rambouillet lambs were randomly assigned to one of three experimental diets (seven lambs per treatment): (1) a control diet (W/o-Salsola) containing 300 g/Kg dry matter (DM) of sorghum stover; (2) a diet with a medium inclusion of Salsola tragus (15-Salsola), which contained 150 g/Kg DM of Salsola tragus and 150 g/Kg DM of sorghum stover; and (3) a diet comprising 300 g/Kg of Salsola tragus (30-Salsola). The results showed there were no differences (p > 0.05) in lamb growth performance during the fattening phase. The in vitro gas analysis demonstrated that the 30-Salsola treatment increased lag time h−1 (p < 0.05) and reduced gas production (p = 0.03). The metabolomic analysis findings suggest that the treatments that included Salsola tragus significantly positively affect the metabolomic composition of meat (p < 0.05). The use of 15-Salsola as a source of forage is promising for feeding lambs during the fattening phase. Full article
15 pages, 2474 KiB  
Article
Evaluation of Creatinine-Based Methods for Estimating the Urine Volume of Lactating and Dry Dairy Cows with Special Consideration of Using Spot Urine Samples
by Katharina Padberg, Ulrich Meyer, Dirk von Soosten, Fabian Billenkamp, Liane Hüther, Christin Unruh, Christian Visscher and Sven Dänicke
Ruminants 2025, 5(3), 31; https://doi.org/10.3390/ruminants5030031 - 16 Jul 2025
Viewed by 235
Abstract
A key factor in calculating dairy cows’ nitrogen (N) excretion is knowing the amount of daily excreted urine. The present study aimed to investigate two methods to calculate the daily urine volume (UV) excreted using spot urine samples. Data were obtained from nine [...] Read more.
A key factor in calculating dairy cows’ nitrogen (N) excretion is knowing the amount of daily excreted urine. The present study aimed to investigate two methods to calculate the daily urine volume (UV) excreted using spot urine samples. Data were obtained from nine balance experiments involving 47 lactating and seven non-lactating German Holstein cows, with an average body weight (BW) of 620 ± 95 kg and an average age of 5.6 ± 1.4 years. Daily urinary creatinine (Cr) and UVs were known for all animals. The first method was developed by linearly regressing the daily excreted amount of Cr in urine against BW (p < 0.001; R2 = 0.51; RSE: 2.8). The slope of the regression was used to calculate UV. The second method includes a non-linear regression of UV on Cr concentration in urine, allowing direct estimation of UV without knowledge of BW (p < 0.001; RSE: 8.13). Both estimation methods were compared to the standard method to determine UV from balance trials using Lin’s concordance correlation coefficient (CCC) and Bland–Altman plots. The first method had a CCC of 0.81, and the second method had a CCC of 0.85. Both methods can confidently be applied to calculate UV. Therefore, the second method is usable if BW is unavailable. Full article
(This article belongs to the Topic The Environmental Footprint of Animal Production)
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12 pages, 869 KiB  
Review
Factors Influencing the Setting of Automatic Teat Cup Removal at the End of Machine Milking in Dairy Cows—An Overview
by Shehadeh Kaskous
Ruminants 2025, 5(3), 30; https://doi.org/10.3390/ruminants5030030 - 1 Jul 2025
Viewed by 248
Abstract
Overmilking occurs when the teat cups remain attached to the udder during milking, even though there is little or no milk flow. This puts pressure on the teat tissue and reduces milk production due to longer milking times, meaning fewer cows are milked [...] Read more.
Overmilking occurs when the teat cups remain attached to the udder during milking, even though there is little or no milk flow. This puts pressure on the teat tissue and reduces milk production due to longer milking times, meaning fewer cows are milked per hour. Therefore, the correct removal of the teat cup at the end of mechanical milking is crucial for the milking process. The aim of this study was to describe the factors influencing automatic teat cup removal (ATCR) at the end of mechanical milking and to demonstrate its importance for udder health, milk production and milk quality. There are considerable differences between milking system suppliers and countries regarding the minimum removal of the teat cup at the end of the milking process. However, to ensure good milk quality, prevent teat damage and reduce the risk of mastitis, it is important to shorten the working time of the milking machine on the udder in both automatic and conventional milking systems. For this reason, several studies have shown that increasing the milk flow switch point effectively reduces milking time, especially in automatic milking systems where dairy cows are milked more than twice a day. However, when the ATCR setting was increased above 0.5 kg·min−1, milk production decreased, and the number of somatic cells in the milk produced increased. Therefore, the use of ATCR at a milk flow rate of 0.2 kg·min−1 significantly increased milk production, improved milk quality and maintained udder health when a low vacuum level (34–36 kPa) was used in milking machines such as MultiLactor and StimuLactor (Siliconform, Germany). In conclusion, ATCR at a milk flow of 0.2–0.3 kg·min−1 is a useful level to achieve various goals on dairy farms when a low vacuum of 34–36 is used in the milking machine. If the milking machine uses a higher vacuum, it is possible to program a higher ATCR at a milk flow of up to 0.5 kg·min−1. Full article
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24 pages, 348 KiB  
Review
Knowledge Gaps in the Nutrient Requirements of Beef Cattle
by Michael L. Galyean, Karen A. Beauchemin, Joel S. Caton, N. Andy Cole, Joan H. Eisemann, Terry E. Engle, Galen E. Erickson, Clint R. Krehbiel, Ronald P. Lemenager and Luis O. Tedeschi
Ruminants 2025, 5(3), 29; https://doi.org/10.3390/ruminants5030029 - 29 Jun 2025
Viewed by 664
Abstract
The 8th revised edition of the Nutrient Requirements of Beef Cattle was released in 2016, with the recommendations provided in the publication being used extensively in both research and production settings. In the context of research needs identified in that publication, our objective [...] Read more.
The 8th revised edition of the Nutrient Requirements of Beef Cattle was released in 2016, with the recommendations provided in the publication being used extensively in both research and production settings. In the context of research needs identified in that publication, our objective was to review research on beef cattle nutrient requirements published since 2016 and identify knowledge gaps that should be addressed. Relative to energy requirements, the effects of environmental temperature and grazing activity, along with stress and disease, on maintenance requirements are inadequately characterized or defined. In addition, relationships between retained energy and protein should be more fully elucidated, and additional guidance on body weight at a target compositional endpoint is needed. Areas of continuing concern include accurately and precisely predicting microbial protein supply, predicting N recycling, and the metabolizable protein requirements for maintenance. Mineral and vitamin requirements are often challenging because of a lack of consistency in models used to determine requirements and potential effects of unique production settings on requirements. Based on recent research with feedlot cattle, zinc and chromium requirements should be examined more closely. Because predictions of dry matter intake are critical to supplying nutrients, additional development of prediction equations is needed, especially for beef cows and grazing beef cattle in general. Given considerable research in prediction of greenhouse gases, reevaluation of 2016 recommendations is warranted, along with a need for the updating of equations to predict excretions of N and P. Composition of feeds, particularly byproducts from ethanol production or other industrial streams, represents a knowledge gap, with obtaining reliable energy values of these feeds being a notable challenge. Nutritional models provide the means to integrate nutrient requirement recommendations into practice, and moving towards mechanistic models that take advantage of artificial intelligence and precision livestock farming technologies will be critical to developing future modeling systems. Full article
13 pages, 567 KiB  
Article
An Observational Study of the Microbiological Quality of Bovine Colostrum Fed to Calves on Three Dairy Farms
by Flávio G. Silva, Marta Laranjo, Severiano R. Silva, Cristina Conceição and Joaquim L. Cerqueira
Ruminants 2025, 5(3), 28; https://doi.org/10.3390/ruminants5030028 - 22 Jun 2025
Viewed by 323
Abstract
This study aimed to evaluate the microbiological quality of colostrum on three dairy farms with different colostrum management hygiene practices and to compare it with the current colostrum quality guidelines. On farm A, colostrum was fed raw, while on farms B and C [...] Read more.
This study aimed to evaluate the microbiological quality of colostrum on three dairy farms with different colostrum management hygiene practices and to compare it with the current colostrum quality guidelines. On farm A, colostrum was fed raw, while on farms B and C it was heat treated. On farms A and B, the feeding equipment was cleaned manually, while on farm C, an automated cleaning system was used. Samples were collected from the calf-feeding equipment and submitted for microbial culture: total plate count (TPC); total coliform count (TCC); and E. coli, enterobacteria (ENTB), staphylococci (STAP), and lactic acid bacteria counts. In addition, pH, water activity (aW), and Brix were analyzed. Colostrum quality was defined as follows: good quality (GQ)—TPC < 100,000, TCC < 10,000, STAP < 50,000 cfu/mL, and Brix ≥ 22%; excellent quality (EQ)—TPC < 20,000, TCC < 100, STAP < 5000 cfu/mL, and Brix ≥ 25%. Mean concentrations were as follows: TPC was 3.99 × 105 cfu/mL (min: 40.00, max: 1.32 × 107 cfu/mL); TCC was 1.17 × 104 cfu/mL (min: <detection limit, max: 6.37 × 105 cfu/mL); and STAP was 1.77 × 104 cfu/mL (min: <detection limit, max: 3.50 × 105 cfu/mL). Approximately 54% (GQ) and 32% (EQ) of samples met the defined criteria. Farm C consistently showed lower microbial counts across all culture types. Colostrum from farm B had lower TCC, LAB, and E. coli counts than farm A but not TPC, STAP, and ENTB. These results showed that a considerable proportion of calves were fed colostrum with suboptimal quality, especially when less rigorous hygiene practices were implemented. Full article
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12 pages, 226 KiB  
Article
Genetic Evaluation of Reproductive and Productive Traits in Zaraibi Goats Under Tropical Climatic Conditions
by Aya Esam Hemada, Heba Abd El-Halim Ahmed, Asmaa Zayed Mohamed, Adel Salah Khattab, Oludayo Michael Akinsola and Thiruvenkadan Aranganoor Kannan
Ruminants 2025, 5(2), 27; https://doi.org/10.3390/ruminants5020027 - 17 Jun 2025
Viewed by 348
Abstract
Understanding the genetic and phenotypic basis of economically important traits is essential for designing effective breeding programs in livestock. This study aimed to evaluate the phenotypic performance and estimate genetic parameters for one reproductive trait—litter size at birth (LSB)—and three pre-weaning growth traits—birth [...] Read more.
Understanding the genetic and phenotypic basis of economically important traits is essential for designing effective breeding programs in livestock. This study aimed to evaluate the phenotypic performance and estimate genetic parameters for one reproductive trait—litter size at birth (LSB)—and three pre-weaning growth traits—birth weight (BW), weaning weight (WW), and average daily gain (ADG)—in a population of 1888 Zaraibi kids born between 2018 and 2023. Genetic parameters were estimated using animal models implemented in the MTDFREML software. The overall least squares means (±standard error) for LSB, BW, WW, and ADG were 2.22 ± 0.02, 2.03 ± 0.01 kg, 10.22 ± 0.05 kg, and 90.00 ± 0.50 g/day, respectively. Statistical analyses indicated that month of birth, year of birth, and type of birth had significant (p < 0.05) effects on all traits, while the sex of the kids had no significant effect (p > 0.05) on LSB. Direct heritability estimates (h2d) derived from Model 1 (including additive genetic, permanent environmental, and residual effects) were 0.13 ± 0.01 for LSB, 0.30 ± 0.04 for BW, 0.38 ± 0.01 for WW, and 0.30 ± 0.10 for ADG. Under Model 2 (which incorporated maternal genetic effects and their covariance with direct genetic effects), maternal heritability (h2m) estimates for LSB, BW, WW, and ADG were 0.05 ± 0.01, 0.15 ± 0.01, 0.12 ± 0.01, and 0.14 ± 0.01, respectively. Overall, the results emphasize the importance of maternal genetic effects in influencing pre-weaning growth traits. Therefore, maternal genetic components should be explicitly considered in genetic evaluation and selection strategies aimed at improving early growth performance in Zaraibi goats. Full article
16 pages, 1446 KiB  
Article
Ethnozootechnical Perspectives on the Decline of Traditional Knowledge About Local Goat and Sheep Breeds in the Semi-Arid Region of Paraíba, Brazil
by Raissa C. Silva, Marilene N. Melo, Carlos F. T. de Oliveira, José V. Cardoso, Luis A. C. Cevallos, Laura L. da Rocha, Janaina K. G. Arandas and Maria N. Ribeiro
Ruminants 2025, 5(2), 26; https://doi.org/10.3390/ruminants5020026 - 13 Jun 2025
Cited by 1 | Viewed by 1013
Abstract
The conservation of local breeds plays a strategic role in maintaining genetic variability, ensuring adaptive responses to environmental challenges, and preserving the cultural and socioeconomic structures of traditional communities. In this context, this study explores the potential disappearance of traditional knowledge about local [...] Read more.
The conservation of local breeds plays a strategic role in maintaining genetic variability, ensuring adaptive responses to environmental challenges, and preserving the cultural and socioeconomic structures of traditional communities. In this context, this study explores the potential disappearance of traditional knowledge about local breeds from an ethnozootechnical perspective. The objectives were (I) to establish the breeding history of goat and sheep breeds/ecotypes in the semi-arid region of Paraíba; (II) to estimate the diversity index; and (III) to evaluate the selection criteria used by local communities in four territories: Coletivo, Borborema, Folia, and Casaco. The study aims to support genetic conservation and improvement programs. Data collection was participatory, involving breeders from all territories. To recover the breeds’ history, questionnaires were applied to the oldest breeders, called the “guardians.” Two workshops were held to assess the diversity of breeds in the past landscape (PP) and current landscape (PA), using the Recall technique. Responses were recorded in spreadsheets for analysis. Descriptive statistics and multiple correspondence analysis (MCA) were used to assess animal distribution. The Shannon index indicated a drop in goat breed diversity, from 1.3 (PP) to 0.87 (PA). For sheep breeds, it decreased slightly from 0.7 to 0.66. Breeders reported valuing traits such as adaptability, disease resistance, fertility, and conformation. Their strong emotional connection with the animals highlights the breeds’ cultural relevance. A strong connection was found between the loss of genetic material in the studied territories and the extinction of local communities’ knowledge about local breeds. Full article
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2 pages, 128 KiB  
Editorial
Recent Research on Livestock Microbiota, Its Role in Digestion and Its Impact on Methane Emissions
by Magdalena Arévalo-Turrubiarte and Ana Isabel Roca-Fernández
Ruminants 2025, 5(2), 25; https://doi.org/10.3390/ruminants5020025 - 11 Jun 2025
Viewed by 298
Abstract
The rumen is the largest compartment of the ruminant stomach and plays a central role in the digestive physiology of bovine, ovine, and caprine species [...] Full article
25 pages, 1328 KiB  
Article
Cytokine Profiling and Puberty Enhancement Post Altrenogest Feeding in Prepubertal Murrah Buffalo (Bubalus bubalis) Heifers
by Sneha Swapna Haridas, Prahlad Singh, Navdeep Singh Ratta, Chanchal Singh and Mrigank Honparkhe
Ruminants 2025, 5(2), 24; https://doi.org/10.3390/ruminants5020024 - 10 Jun 2025
Viewed by 569
Abstract
Cytokine and Anti-Müllerian Hormone (AMH) profiling were performed in prepubertal Murrah buffalo heifers’ post-progesterone supplementation and Co-synchronization (Co-synch) protocol. Cytokine (IFNγ, IL6, IL1, IL13, TNFα, and TGFβ), AMH, progesterone, and estrogen hormone estimations were conducted. The ovarian follicular pattern and fertility outcome were [...] Read more.
Cytokine and Anti-Müllerian Hormone (AMH) profiling were performed in prepubertal Murrah buffalo heifers’ post-progesterone supplementation and Co-synchronization (Co-synch) protocol. Cytokine (IFNγ, IL6, IL1, IL13, TNFα, and TGFβ), AMH, progesterone, and estrogen hormone estimations were conducted. The ovarian follicular pattern and fertility outcome were recorded. Eighteen prepubertal heifers, 15–17 months of age, 250–300 kg body weight (BWt) were randomly divided into three groups, Group 1: n = 6, supplemented with altrenogest 0.044 mg/kg BWt/day/heifer orally for 14 days along with Co-synch program; Group 2: n = 6, implanted Controlled Internal Drug Release (CIDR) for 14 days and co-synch program, Group 3: n = 6, received Co-synch program. Ultrasonography was performed to determine ovarian follicle status on respective days of sampling. Pregnancy diagnosis was performed around 45 and 60 days post artificial insemination (AI). The Estradiol 17-β level remained constant in Group 1, Group 2, and Group 3 during the progesterone treatment and Co-synch treatment. Estradiol levels during Co-synch were significantly less (p = 0.024) on day 9 of Co-synch (14.41 ± 1.97 pg/mL) than on day 0 (20.11 ± 0.36 pg/mL) and on day 7 (19.77 ± 0.34 pg/mL) in prepubertal buffalo heifers in Group 1. However, no significance was observed in other groups. Progesterone levels in buffalo heifers subjected to synchronization protocols varied significantly (p < 0.05) on day 7 of progesterone (P4) treatment between Group 1, Group 2, and Group 3. Supplementation of altrenogest showed a significant (p = 0.043) increase in progesterone levels by day 14 of altrenogest treatment. Progesterone varied significantly in all groups on day 9 of Co-synch protocol [Group 1 (p = 0.020), Group 2 (p = 0.041), and Group 3 (p = 0.007)]. Cytokine IFNγ showed high correlation with progesterone, indicating the role of IFNγ in puberty in buffalo heifers (r = 0.626, p < 0.01). Anti-Müllerian Hormone had a significant positive correlation when supplemented with altrenogest with IFNγ (r = 0.673, p < 0.01) and TGFβ (r = 0.463, p < 0.01), whereas it was a negatively correlated with TNFα (r = −0.34, p < 0.05). Based on Karl Pearson correlation coefficients, IL13 and TGFβ could be considered as markers for puberty in buffalo heifers. Full article
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13 pages, 275 KiB  
Article
Addition of Exogenous Fibrolytic Enzymes to the Feed of Confined Steers Modulates Fat Profile in Meat
by Rafael Vinicius Pansera Lago, Joana Morais da Cruz, Gabriel J. Wolschick, Mateus H. Signor, Michel Breancini, Bruna Klein, Luiz Eduardo Lobo Silva, Roger Wagner, Maria Eduarda Pieniz Hamerski, Gilberto V. Kozloski and Aleksandro Schafer da Silva
Ruminants 2025, 5(2), 23; https://doi.org/10.3390/ruminants5020023 - 9 Jun 2025
Viewed by 425
Abstract
The objective of this study was to evaluate whether the addition of fibrolytic enzymes to the diet of cattle in confinement improves feed digestibility weight gain; as well as evaluating the profile of fatty acids in the ruminal environment and in meat fatty [...] Read more.
The objective of this study was to evaluate whether the addition of fibrolytic enzymes to the diet of cattle in confinement improves feed digestibility weight gain; as well as evaluating the profile of fatty acids in the ruminal environment and in meat fatty acids profile. In total, 24 male dairy cattle (Holstein) aged 8 months and weighing an average of 212 kg, were divided into 2 groups: control (without additive) and treatment (0.25 g of enzymes/kg of dry matter of total diet). The experiment lasted 120 days. The first 20 days are allocated for the adaptation period. During the study, samples of blood, ruminal fluid, and feces were collected, as well as weighing the cattle and measuring their daily feed consumption. There was no effect of treatment on body weight, feed intake, feed efficiency, and nutrient digestibility (p > 0.05). Cholesterol levels were higher in the serum of cattle that consumed the enzyme; serum amylase activity was higher in cattle that received the additive only on day 120 of the experiment (p < 0.05). There was a greater amount of volatile fatty acids in the ruminal fluid, combined with a greater amount of acetic acid. The amount of fat in the meat of cattle that consumed fibrolytic enzymes was higher compared to the control group (p < 0.05). Meat from cattle in the treatment group had lower amounts of saturated fatty acids and higher amounts of unsaturated fatty acids (p < 0.05). The fibrolytic enzymes addition altered rumen fermentation in such a way that lipid metabolism was changed, which had a serious impact on cholesterol and tissue levels, that is, in the meat that had a greater amount of total lipids, an unsaturated fat. Full article
(This article belongs to the Special Issue Nutrients and Feed Additives in Ruminants)
11 pages, 269 KiB  
Article
The Effect of a Pre-Mix of Essential Organic Minerals on Growth, Antioxidant Indices, and the Diarrhea Incidence in Dairy Calves Breed in Arid Climates
by M. S. Mortazavi, M. Hajmohammadi, Giovanni Buonaiuto, Riccardo Colleluori, Martina Lamanna, Damiano Cavallini, R. Valizadeh, S. H. Ebrahimi and C. A. F. Oliveira
Ruminants 2025, 5(2), 22; https://doi.org/10.3390/ruminants5020022 - 30 May 2025
Viewed by 891
Abstract
This study evaluated the effects of organic trace mineral supplementation on growth performance, antioxidant indices, mineral status, and diarrhea incidence in dairy calves raised in arid climates. Twenty-five male Holstein calves were randomly assigned to five dietary treatments for 21 days, as follows: [...] Read more.
This study evaluated the effects of organic trace mineral supplementation on growth performance, antioxidant indices, mineral status, and diarrhea incidence in dairy calves raised in arid climates. Twenty-five male Holstein calves were randomly assigned to five dietary treatments for 21 days, as follows: (1) control group (no organic mineral supplementation), (2) copper-methionine (Cu-Met) supplemented diet, (3) zinc-methionine (Zn-Met) supplemented diet, (4) manganese-methionine (Mn-Met) supplemented diet, and (5) Zn-Met + Cu-Met + and Mn-Met in a premix supplemented diet. Mineral supplementation had no effect on final body weight or average daily gain. However, the concentrations of Zn, Cu, and Mn significantly increased (p < 0.01) in blood and feces of treated animals. The highest blood concentrations of Zn and Mn were observed in calves receiving Zn-Met, while Mn-Met supplementation significantly influenced blood Cu levels. The highest Zn excretion was recorded in calves receiving the organic mineral premix, whereas the highest Mn and Cu excretion was observed in the Cu-Met group. Additionally, mineral supplementation enhanced total antioxidant capacity and superoxide dismutase activity in plasma samples (p < 0.01). These findings suggest that organic mineral supplementation could be an effective strategy to improve mineral bioavailability and support the health of dairy calves during early life in arid climates. Full article
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