Search Results (313)

Butterworts (Pinguicula spp.) are carnivorous plants often restricted to small, isolated populations in mountainous microclimates, making them vulnerable to inbreeding and demographic decline. In Mexico, the global hotspot for Pinguicula diversity (>50 species, 90% endemic), conservation is limited by scarce data on population biology, demography, and vulnerability. This study provides the first population size estimate for the endemic P. gracilis and evaluates how patch and population size influence biological fitness components across three localities in northeastern Mexico. Using a multi-component fitness framework and statistical models, we found that smaller populations and patches exhibit significantly reduced reproductive fitness, particularly in fruit set and seed quality. Patch size showed strong positive correlations with fruit production (r = 0.45–0.53), while isolation negatively affected seed output (r = −0.48). These results highlight the role of landscape structure in shaping fitness components, with patch size influencing reproductive effort and connectivity determining reproductive success. Our findings provide the first germplasm and population baseline for P. gracilis and a framework to identify vulnerable populations, prioritize conservation actions, and guide restoration strategies. Full article

Managing tigers (Panthera tigris) under human care poses significant risks, particularly with individuals exhibiting aggressive behaviors. Although operant conditioning is widely recommended, structured protocols for reducing aggression in large carnivores remain scarce in the literature. This study investigated the efficacy of a constructional approach, using negative reinforcement as a methodological bridge to positive reinforcement in three adult tigers with a history of generalized aggression. The intervention was based on functional distance applied through negative reinforcement by the trainer’s withdrawal as a consequence of the desired behavior, where the researcher’s presence acted as a functional stimulus and strategic retreat was contingent upon micro-behaviors of calmness. This methodology allowed the animals to exert control over the social interaction, facilitating a systematic transition to positive reinforcement and strengthening voluntary approximation. The results indicated a significant reduction in agonistic responses and a rapid transition toward cooperative behaviors. This work provides detailed evidence on how the constructional approach can overcome food refusal and establish a secure training foundation. We conclude that the protocol offers a replicable methodological framework for managing aggression in wild animals initially resistant to traditional methods, promoting safety and welfare through structured behavioral interactions. Full article

Otters spend a considerable amount of time vocalizing within zoos and other captive settings. Although associations between these vocalizations and social activity have been investigated prior, it is not always clear how this is reflected in interactions with people under typical zoo conditions, including interactions with zoo visitors and staff. In addition, the implications for zoo welfare and their relation to vocalizing are also not clear, warranting greater investigation to identify potential future welfare metrics for otter species. We examined zoo Asian small-clawed otter vocalizations and general activity in the presence of people, including both visitors and staff. Our focus was on measuring vocalizations as a binomial response (yes/no) in relation to two variables: (a) people, and (b) time. In addition, we also measured differences in vocalizations during sessions, when visitors were present with or without staff. Finally, we measured general otter activity during sessions when 50% or more of the observation intervals had people present (More People), as opposed to sessions where less than 50% of all intervals had a person present (Less People). Overall, otters were statistically more active and less out of sight when more people were present; a weak to moderate correlation was observed between vocalizations and people, a weak correlation was observed between vocalizations and time, and there was no statistically significant difference in vocalizations of the otters in the presence of visitors regardless of staff presence. These results suggest that the measured variables showed no evidence of an overtly negative visitor effect for the otters and may have produced a potentially enriching effect. In addition, measuring multiple variables, including time of day, diurnal patterns, feeding patterns, weather, and providing experimental control could aid in identifying the potential visitor effect for otters and other zoo animals. Full article

Probiotics are widely studied as antibiotic alternatives in commercial aquaculture, yet their effects on fish maintained under long-term aquarium conditions remain poorly understood. This study addressed this gap by evaluating dietary Escherichia coli Nissle 1917 (EcN) supplementation on gut microbiota and inflammatory cytokine expression in hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) from a recirculating aquarium system. In this study, hybrid grouper were maintained in triplicate tanks under long-term aquarium environments, and fed a basal diet with 1 × 10⁸ CFU/g EcN (SS group) or a control diet (CS group) for 28 consecutive days. Based on 16S rRNA high-throughput sequencing and qPCR, the intestinal microbiota and expression levels of IL-4, TNF-α, and IL-1β were measured. At the phylum level, the relative abundance of Firmicutes increased from 15.63% (CS) to 66.70% (SS), while Proteobacteria decreased from 76.77% to 30.61%. At the genus level, Exiguobacterium became the dominant taxon in the SS group. Furthermore, EcN supplementation significantly upregulated IL-4 expression and downregulated TNF-α and IL-1β expression. EcN supplementation significantly altered gut microbiota composition, with marked changes in community structure and notable shifts in dominant taxa. Thus, this study provides one of the investigations into EcN-mediated restructuring of intestinal bacterial communities and modulation of host immune transcriptional responses in hybrid grouper maintained under controlled aquarium settings. These findings offer a foundation for designing microbiome-targeted interventions in captive marine fish systems. Full article

Empathy toward animals has received increasing attention because of its relationship to prosocial attitudes, conservation engagement, and environmental concern. Despite growing interest, the way empathy toward animals is measured varies widely across disciplines and applied contexts, making it difficult to compare findings or assess the strength of existing instruments. This paper examines the measurement landscape of empathy toward animals by identifying and describing tools used in both academic research and conservation practice. A search of Web of Science yielded 2155 unique records, resulting in a final sample of 65 peer-reviewed studies with empathy assessment instruments published between 2000 and 2025. These were supplemented by 42 instruments shared by members of the Advancing Conservation through Empathy for Wildlife (ACE for Wildlife^®) Network, one of the largest known networks of professionals focused on enhancing and evaluating empathy toward animals. Across these sources, we observe substantial variation in how empathy is operationalized, including differences in construct emphasis, focal species, intended audiences, and attention to reliability and validity. Academic studies primarily use surveys emphasizing affective empathy toward mammals, whereas practitioner-developed tools are more diverse and often assess cognitive and motivational dimensions across cohort groups. In mapping differences in approaches, we identify persistent gaps and provide suggestions to better align scholarly and applied assessment tools. Full article

Moving zoo animals to new enclosures is a normal part of zoo management, but little is known about how animals respond to these changes. Understanding how species adjust after a move can help zoos improve animal management and support smoother transitions between enclosures. This study investigated the behaviour and enclosure use of 121 Chilean flamingos at WWT Slimbridge after they moved from an enclosure that may have housed flamingos for up to 60 years into a newly designed environment. Animal care staff photographed the flock once daily before afternoon feeding and recorded the number of birds foraging, moving, preening, or inactive. The presence of wild birds, visitors, and staff was also recorded to assess possible influences on behaviour. Behaviour in the new enclosure was compared with activity levels previously recorded in the former enclosure. Results showed that flamingos spent more time foraging in the first month post-move and were more often seen in their pool. Activity did not differ between the former and new enclosures. This research provides important information on how flamingos respond to a housing change, showing that birds are more explorative of their new environment before settling into a previously documented routine. Flamingo preening showed an association with wild birds (increase preening) and visitor presence (reduced preening). Such human influences should be further investigated to determine whether flamingos change behaviour due to visitors or whether flamingo activity draws more visitors to the exhibit. Ultimately, our research shows that flamingos settle quickly into a new environment and do not display obvious signs of discomfort when a move is well planned and when enclosure modifications consider species’ natural history. We show the usefulness of a quick-and-easy data collection protocol, implemented by animal care staff, to the continual monitoring and evaluation of zoo husbandry. Full article

Itraconazole is an antifungal drug used to treat chytridiomycosis, one of the leading causes of global amphibian decline in species such as the critically endangered Panamanian golden frog (Atelopus zeteki). Despite its widespread use for prophylaxis and treatment in both assurance colonies and free-ranging amphibians, there is minimal pharmacologic information to guide dosing. In experiment A, frogs were exposed to 0.01% itraconazole for 10 min and tissue samples were analyzed at various time points from 1 to 84 h. In experiment B, frogs were divided into six groups and exposed to itraconazole in different combinations of concentration (0.01% or 0.001%) and time (5, 10, or 15 min) over 10 days of treatment. Tissue concentrations were quantified via high-performance liquid chromatography. In experiment A, following a one-time dose, itraconazole concentrations remained high until the end of the experiment at 84 h. In experiment B, at 0.01% itraconazole daily for 10 days, skin and liver concentrations were high and increased substantially over the 10-day treatment course. Frogs exposed at the lower concentration (0.001%) had tissue concentrations that appeared to remain steady. At the reported doses over 10 days, there was no histologic evidence of hepatic toxicity, although one frog was found dead in the low-dose bath at 84 h and could not be further analyzed. This experiment is an excellent example of assurance colonies providing evidence-based information for the improved care and welfare of amphibians in order to prepare for future free-ranging populations. Full article

In today’s tourism industry, service quality is essential for differentiation and achieving sustainable competitive advantage. While much research on service quality exists for the private sector, a notable gap remains regarding service dynamics in non-profit public recreational spaces in emerging economies. This study fills that gap by examining service quality (SQ) at National Botanical Gardens (NBGs) in South Africa, focusing on visitor perceptions of SQ importance and performance. Using an adapted Importance–Performance Analysis (IPA) framework, the research explores how visitor expectations compare with actual experiences. A quantitative approach was adopted, with structured questionnaires given to a convenience sample of visitors at key gardens like Pretoria and Walter Sisulu NBGs. Data analysis centred on correlation coefficients to assess the relationship between perceived importance and actual performance. The findings reveal a generally strong, statistically significant positive correlation; however, some attributes show a ‘weak correlation,’ indicating critical service gaps often overlooked by traditional management models. These insights demonstrate that, in natural attraction settings, visitor-perceived importance primarily drives satisfaction with performance. In addition to offering the South African National Biodiversity Institute (SANBI) a strategic plan to boost visitor retention, this study advances the academic discussion by challenging existing SQ models within non-profit recreational sectors. It lays a vital empirical groundwork for future research on public-sector service excellence in the Global South. Full article

The rare and threatened pleurocarpous semi-aquatic moss Drepanocladus sendtneri (Amblystegiaceae) was the focus of an integrative conservation approach aimed at improving knowledge of its biological and ecological characteristics and enhancing its survival prospects. The results provide insights into both the axenic and xenic propagation of this species, as well as its biomass production under ex situ conditions. The KNOP medium proved to be the most suitable for propagation, particularly when demeristemized shoot tips were cultured in an upright orientation. Exogenous application of IBA increased the production of new shoots and reduced the time required to obtain substantial biomass under axenic conditions. Following successful acclimatisation to controlled xenic laboratory conditions, the moss was able to fully develop and spread in experimental basins maintained under outdoor botanical garden conditions, with humidity carefully regulated during dry periods. Within one year, a small number of initial plantlets expanded to cover approximately 4 dm², spreading efficiently over rainwater-soaked filter paper covered with an inert plastic mesh. These results provide practical guidance for the production and ex situ maintenance of D. sendtneri, thereby supporting the development and improvement of conservation action plans for this rare and threatened moss species. Full article

Understanding how environmental enrichment influences behavioural time allocation is particularly important for threatened primate species maintained under human care. Accordingly, we investigated whether environmental enrichment (EE) influences behavioural time allocation in yellow-breasted capuchin monkeys (Sapajus xanthosternos), aiming to inform evidence-based husbandry practices in zoological settings. Employing the standard ethological approach of behavioural coding, we observed 20 capuchins housed in three groups comprising adult and juvenile males and females. We recorded behavioural categories including: aggressive, exploratory, affiliative/play, general activity, alert, inactivity, and abnormal behaviour. To evaluate individual engagement with EE, we applied the ABA paradigm, wherein phases A1 and A2 (controls) represented standard zoo conditions, while phase B corresponded to the implementation of an EE programme. Each phase spanned 10 days, and behavioural data were collected via focal animal sampling (2 × 10 min focal sessions per animal per day), resulting in a total of 1200 focal sessions. Behavioural time allocation was analysed using a multivariate generalized linear mixed modelling approach that accounted for the interdependence among behavioural categories. Based on previous studies, we predicted that environmental enrichment may promote higher levels of play and exploration and lower aggression and inactivity. However, despite by-eye suggestions of increases in play and decreases in activity during enrichment, when behavioural categories were analysed simultaneously within the multivariate framework, overall behaviour time budgets and behavioural diversity were found not to change significantly across experimental phases. There were also no sex or age effects on behaviour. This indicates that for S. xanthosternos, the enrichment protocol used here did not provide sufficient novelty or complexity to alter established activity patterns. Integrated analytical approaches are needed to further evaluate the effectiveness of enrichment strategies to ensure they are tailored to specific cognitive and social needs of complex species; future studies could explore how social dynamics, enclosure design, and environmental complexity interact to shape behavioural responses to enrichment. Full article

Lesser hedgehog tenrecs (Echinops telfairi) are small, nocturnal insectivores from Madagascar that exhibit hibernation and brief torpor bouts. While multiple studies have investigated torpor physiology, no studies have focused on torpor in tenrecs maintained under human care or their recommended husbandry. We surveyed 96 institutions (71.9% response rate) housing 172 tenrecs to assess husbandry, torpor, handling, and associated weight changes. Most institutions reported that torpor occurred annually, typically between October and April, and lasted approximately 5–6 months. Weight distributions differed significantly pre- versus post-torpor, with females and males losing 34.4 g and 20.9 g on average, respectively. Females were heavier than males before and after torpor, the first report of a sex-based weight difference in this species. Most institutions used tenrecs as ambassador animals, and approximately half continued educational programming during torpor. Tenrecs handled more frequently during torpor tended to gain more weight, likely due to increased energy expenditure and compensatory feeding. Tenrecs entered torpor regardless of daylight, temperature, or humidity, with no significant husbandry differences between torpor- and non-torpor-reporting institutions. Torpor is a critical physiological adaptation supporting energy conservation and species survival. Its expression should be supported through appropriate environmental conditions, diet, minimal disturbance, and monitoring. Full article

Kings Park and Botanic Garden is a globally recognised botanic garden and research institution in the biodiversity hotspot of southwest Western Australia. A history of the contribution of staff and researchers at Kings Park and Botanic Garden to the knowledge and promotion of the flora of Western Australia is provided. Through an analysis of the published literature, reports, and correspondence with former staff, a strong history of collaborative, world-class research is outlined. This paper focuses on the contributions of staff and students who have discovered and promoted Western Australian flora from the planning and establishment of the Botanic Garden in 1961 up to 2015. Early research was conducted by a small number of individuals with structured research programs emerging much later. Significant contributions have been made in the fields of plant conservation, plant ecology, seed biology, ecosystem restoration, plant systematics and mycorrhizal associations. Key partnerships with universities, other government organisations and industry have been fundamental to the focus and success of research programs. Full article

Modern botanical gardens are essential for conservation, research, education, and recreation. However, recreating habitats with extreme edaphic conditions, such as the Iberian gypsum steppes (priority habitat 1520), poses a significant challenge due to the severe physicochemical constraints of gypsisols. This work aimed to present and evaluate a biomimetic protocol for establishing two gypsum botanical gardens in the southeast Iberian Peninsula, one on a university campus and one at a mining concession, to fulfil all four prototypical functions. The design was biomimetic, replicating the floristic (Gypsophiletalia scrublands) and edaphic characteristics of natural gypsum areas. Crucially, gypsum-milling waste (fines) from the mining operation was repurposed as the main substrate to create the artificial gypsisols. Physicochemical analyses confirmed this strategy effectively replicated the key chemical properties of natural gypsisols, including high CaSO₄ concentration, pH, and electrical conductivity, although the artificial soils displayed the low carbon and nitrogen content typical of disturbed gypsum soils. The gardens successfully fulfilled their conservation role by maintaining populations of endemic and threatened gypsophilous species, which flowered and set fruit. The industrial garden validated a research function by serving as a platform for the successful translocation of threatened Narcissus tortifolius bulbs. This project validates a replicable, biomimetic technical protocol that transforms a mining residue into a functional substrate for conservation. The dual model (academic/industrial) maximizes the botanical garden’s functions, offering an effective and highly visible strategy for conserving gypsum biodiversity and countering the social undervaluation of these extreme ecosystems. Full article

This study presents an innovative and interdisciplinary didactic approach for teaching biodiversity to high school students, aiming to enhance basic learning skills and promoting deeper understanding of biological concepts. The designed educational proposal aims to support policy-driven collaboration between schools and botanical gardens within the framework of coordinated and long-term educational policies. The intervention was designed to cultivate both cognitive and metacognitive skills within three (3) didactic hours, offering a holistic learning experience through the study of Tulipa members used as an alluring model. A total of 168 Greek first- and second-grade high school students (on average 16 years old) participated in the study. Biodiversity was examined in a multidisciplinary fashion, focusing on species’ diversity (phenotypic), genetic-karyological diversity, and habitat diversity. The core components of the approach included: (a) the completion of a corresponding worksheet module, which effectively engaged students in the learning process using the 5E didactic model, and (b) a karyotype lab experiment using living Tulipa specimens. The learning process was evaluated through two questionnaires assessing the acquisition of scientific knowledge and behavioral outcomes. The results showed a positive impact on students’ understanding regarding the genetic material and biodiversity, with the karyotype experiment playing a key role in achieving high performance in both cognitive and affective learning objectives. Knowledge scores were higher in the second-grade students (53–73%) than in the first-grade students (44–69%) of high school, especially regarding concepts such as karyotype applications and biodiversity-ecosystem balance. The karyotype experiment positively correlated with both the evaluation of the intervention and the shifts in biodiversity beliefs (Pearson’s r = 0.649, 0.515; p < 0.05, respectively). The modeled inquiry-based approach with living tulips and karyotype experiments can help schools and botanic gardens counteract plant blindness by enhancing cognitive and affective learning outcomes within a limited instructional timeframe. Full article

Historic gardens are dynamic archives of biodiversity and cultural heritage, yet many have been lost or transformed, erasing their original floristic identity. This study reconstructs the experimental agricultural garden of Villa Santa Maria in Potenza (Southern Italy). The garden was founded in 1823 by the Società Economica di Basilicata as a provincial center for agronomic innovation. Through a multidisciplinary approach—archival research, iconographic analysis, and taxonomic revision—we analyzed the Catalogus Plantarum Horti Agrarii Lucani (1846), which listed 670 cultivated taxa, including 196 varieties. Updated nomenclature confirmed the presence of 450 plant taxa. The garden featured a clear functional zoning system integrating ornamental parterres, orchards, nurseries, artificial meadows, and experimental plots for industrial crops and forage species. Chorological analysis reveals a dominant Mediterranean component, along with significant Asiatic and American elements, and smaller Australian and African contributions, reflecting 19th-century plant exchange networks. The conservation assessment shows that many historically cultivated taxa are now nationally protected, and several endemics retain high ecological value. These results highlight the role of historic gardens as living laboratories for biodiversity conservation and cultural continuity. Reintroducing documented species and heritage cultivars within a conservation framework can strengthen urban resilience and identity. This case exemplifies how botanical memory can guide landscape planning by linking historical authenticity with contemporary ecological and educational objectives. Full article