Search Results (13)

The fully polarimetric aperture synthesis radiometer (FPASR) is capable of acquiring the fully polarimetric brightness temperature (BT), which has become increasingly significant in remote sensing. Antenna pattern errors can introduce significant errors to the reconstructed image of the FPASR. Analyzing and correcting the antenna pattern errors is crucial for obtaining high-quality BT images. In this paper, the antenna pattern errors are analyzed and classified into additive and multiplicative errors. A two-step correction method is proposed to reduce the influence of antenna pattern errors on the reconstructed BT. An end-to-end simulator for FPASR has been developed to assess both the antenna pattern errors and the effectiveness of the correction method. The simulation results show that the two-step correction method can reduce the brightness temperature error caused by the antenna pattern errors by over 70%. The successful image of the flight experiment validates the correction method as well. Full article

The Ascomycete genus Cordyceps affects plant crops significantly, filling an important ecological niche. Cordyceps militaris (L.) Fr. presents many health benefits for humans, but its population history has not been reported. The objective of this research was to report the collection, population structure, demographic history, diversity, and cytosine deaminases of 43 wild strains of C. militaris in China through resequencing using an Illumina HiseqTM platform. All strains were assigned to the warm, subtropical, and middle temperate zone populations, confirmed by ADMIXTURE-1.3.0, PCA, and phylogenic analysis. Their population sizes declined historically, suggesting that this species suffered from bottlenecks in the wild. LD decays (r²) revealed a north-to-south migration pattern of wild C. militaris, consistent with the MSMC2-v2.1.4 analysis. The regions of high Pi were aggregating at the chromosomes CP023325.1 (51) and CP023323.1 (9), playing a key role in adaptation, especially for the sites on cytosine deaminase. Within the species, genetic differentiation was relatively high among the three populations (F_st = 0.083, 0.092, and even 0.109). According to the artificial intelligence-assisted (RoseTTAFold) predicted structures of the cytosine deaminases, they were classified into eight clades with unique, distinct, and structurally conserved domains, offering a potential suite of single- and double-stranded deaminases of great promise as tunable base editors for therapeutic and agricultural breeding applications. These provided new insights for mining novel proteins from macrofungi, structurally and functionally. Full article

Since the characteristics of plug seedlings affect the effectiveness of automatic transplanting, this study aimed to explore the effect of the addition of biochar into substrates on the growth of plug seedlings before and after transplanting. The physicochemical properties of substrates with 0%, 5%, 10%, 15%, 20%, and 25% biochar addition all met the requirements of seedling cultivation. The growth trend, root systems, and mechanical properties of seedlings before transplanting and the leaf gas exchange parameters of seedlings after transplanting were measured in this study. The results indicated that the seedlings cultivated with 10% biochar added to the substrate achieved the best growth trend and physiological indices, and the root systems under this treatment were also stronger than those of other treatments, while the seedlings cultivated with 25% biochar treatment were the worst, with less than 22.23% of the growth seen in the 10% biochar treatment, and even less than 1.5% of the growth of the seedlings cultivated without biochar treatment. Since the strong root systems could enhance the mechanical properties of seedling pots, the seedling pots cultivated with 10% biochar added into the substrate possessed the best compression resistance properties, with the maximum value of 49.52 N, and could maintain maximum completeness after free-fall impacting, wherein the loss of root and substrate was only 8.22%. The analysis results of seedlings cultivated after impacting proposed that the seedlings with better growth trends and root systems before transplanting could obtain better leaf gas exchange parameters during the flower stage after transplanting, so the seedlings cultivated with 5%~10% biochar added into the substrate grew better after impacting and then transplanting. It was noticed that the seedlings cultivated with appropriate biochar added into the substrate were able to achieve the optimal growth parameters and mechanical properties before and after transplanting, which were better able to meet the requirements of automatic transplanting. Thus, this study can promote the development of automatic transplanting technology to some extent. Full article

There exists an inaccurate measurement problem in permanent magnet synchronous motors (PMSMs) due to low motor speed operation, high temperatures and humid environments, which will degrade the motion performance and stability of PMSMs. In this study, a model reference adaptive system without position measurement is presented in a PMSM to improve the output performance with external disturbance suppression caused by an environmental change. Firstly, a PI adaptive estimation law is designed to identify the motor speed. Then, a optimization method based on the sliding mode variable structure technique is proposed to realize the stability augmentation of the speed loop by using the parametric fuzzy logic design. To reject the current loop noise, an extended Kalman filter (EKF) is adopted to compensate the input signal in the current loop. The effectiveness of this proposed method is verified via a numerical simulation in the case of different speeds and external loads. Full article

Russula griseocarnosa, an edible and medicinal mushroom abundant in nutrients and notable bioactivities, is predominantly grown in the broad-leaved forest with trees of the family Fagaceae in southern China. This species forms ectomycorrhizal associations with plant roots and cannot be artificially cultivated currently. Previous research indicates a strong correlation between the growth of R. griseocarnosa and factors such as the host plant, climate variables (specifically mean temperature and precipitation from June to October), and the rhizosphere microbiota of its habitat. However, comprehensive studies on the fundamental biology of this species are lacking. The interaction between R. griseocarnosa and its host plant, as well as the mechanisms underlying the microbial community dynamics within its habitat, remain ambiguous. The limited repertoire and diversity of carbohydrate-active enzymes (CAZymes) in R. griseocarnosa relative to saprophytic fungi may contribute to its recalcitrance to cultivation on synthetic media. The specific core enzyme and the substances provided by the host plant to facilitate growth are yet to be elucidated, posing a significant challenge in the artificial cultivation of R. griseocarnosa. The habitat of R. griseocarnosa harbours unique microbial communities, indicating the presence of potentially beneficial microorganisms that could be exploited for artificial propagation and conservation efforts. However, the lack of definitive functional verification experiments hinders the realization of this promising prospect. This review offers a comprehensive overview of the nutritional profile and health benefits of R. griseocarnosa, emphasizing recent developments in its isolation, molecular ecology, and artificial cultivation. Additionally, it explores prospective advancements in R. griseocarnosa research, aiming to enrich our foundational understanding for applied purposes and fostering progress in the realm of ectomycorrhizal edible mushrooms. Full article

For an aperture synthesis radiometer (ASR), the visibility and the modified brightness temperature (BT) are related to the Fourier transform when the distance between the system and the source is in the far-field region. BT reconstruction can be achieved using G-matrix imaging. However, for ASRs with large array sizes, the far-field condition is not satisfied when performing performance tests in an anechoic chamber due to size limitations. Using far-field imaging methods in near-field conditions can introduce errors in the images and fail to correctly reconstruct the BT. Most of the existing methods deal with visibilities, converting near-field visibilities to far-field visibilities, which are suitable for point sources but not good for extended source correction. In this paper, two near-field imaging methods are proposed based on the near-field distance. These methods enable BT reconstruction in near-field conditions by generating improved resolving matrices: the near-field G-matrix and the F-matrix. These methods do not change the visibility measurements and can effectively image both the point source and the extended source in the near field. Simulations of point sources and extended sources in near-field conditions demonstrate the effectiveness of both methods, with F-matrix imaging outperforming near-field G-matrix imaging. The feasibility of both near-field imaging methods is further validated by carrying out experiments on a 10-element Y-array system. Full article

In mirrored aperture synthesis (MAS), the antenna array determines the rank of the transformation matrix connecting the cross-correlations to the cosine visibilities. However, the transformation matrix is rank-deficient, resulting in errors in the reconstructed brightness temperature (BT) image. In this paper, the signal propagations for the vertically polarized wave and horizontally polarized wave are analyzed. Then, the optimization model of the antenna array based on dual-polarization is established. The optimal array configurations are presented, with the corresponding transformation matrices being almost column full ranks. Simulation results demonstrate the validity of the proposed optimization model. Full article

Ganoderma lucidum is an important medicinal mushroom widely cultured in Asian countries. Exopolysaccharides are bioactive compounds of G. lucidum with health benefits. Limited exopolysaccharide content hinders its extraction from G. lucidum. The addition of Tween80 had an enhanced effect on G. lucidum exopolysaccharide production in submerged fermentation. However, the mechanism of this effect remains unclear. In this study, we report on a high-quality assembly of G. lucidum strain yw-1-5 to lay the foundation for further transcriptome analysis. The genome sequence was 58.16 Mb and consisted of 58 scaffolds with an N50 of 4.78 Mb. A total of 13,957 protein-coding genes were annotated and Hi-C data mapped to 12 pseudo-chromosomes. Genes encoding glycosyltransferases and glycoside hydrolases were also obtained. Furthermore, RNA-seq was performed in a Tween80-treated group and control group for revealing the enhanced effect of Tween80 on exopolysaccharide production. In total, 655 genes were identified as differentially expressed, including 341 up-regulated and 314 down-regulated. Further analysis of differentially expressed genes showed that groups of MAPK, amino sugar and nucleotide sugar metabolism, autophagy, ubiquitin-mediated proteolysis, peroxisome, starch and sucrose metabolism, TCA cycle, glycolysis/gluconeogenesis KEGG pathway, glycosyltransferases and glycoside hydrolases played important roles in the enhanced effect of Tween80 on exopolysaccharide production. This work provides a valuable resource for facilitating our understanding of the synthesis of polysaccharides and accelerating the breeding of new strains with a high content of exopolysaccharides. Full article

When observing the Earth from space, the synthetic aperture radiometer antenna array is sometimes set as a non-uniform array. In non-uniform synthetic aperture radiometer image reconstruction, the existing brightness temperature image reconstruction methods include the grid method and array factor forming (AFF) method. However, when using traditional methods for imaging, errors are usually introduced or some prior information is required. In this article, we propose a new IASR imaging method with deep convolution neural network (CNN). The frequency domain information is extracted through multiple convolutional layers, global pooling layers, and fully connected layers to achieve non-uniform synthetic aperture radiometer imaging. Through extensive numerical experiments, we demonstrate the performance of the proposed imaging method. Compared to traditional imaging methods such as the grid method and AFF method, the proposed method has advantages in image quality, computational efficiency, and noise suppression. Full article

Liquefied natural gas (LNG) is the cleanest fossil fuel available, producing less carbon emissions and fewer pollutants than other fossil fuels. Marine transportation is a key process in the LNG supply chain. The use of tank containers, which are portable equipment, can effectively facilitate multi-mode transportation. LNG evaporation causes pressurization, which is a safety concern during transportation. Ship motion and environmental temperature are the main factors affecting pressure variations. In this study, the effect of ship motion on pressurization and holding time was investigated through three types of experiments, namely, prototype, field, and self-pressurization experiments. The results showed that while increased boil-off gas was generated due to ship motion, this evaporation remained stable in dynamic cases. Higher evaporation rates were obtained under more severe dynamic conditions, and the holding time was shortened. The two different effects of ship motion on pressure development discussed here are the facilitation of pressurization due to the enhancement of heat transfer and the prevention of pressurization due to gas condensation at the vapor–liquid interface. These two effects show varied levels of predominance over the pressure variations depending on different stages of transportation. The holding time in the experiments was able to reach 87 days under the most severe condition, which is long enough for long-term shipping; the safety of transporting LNG in tank containers is further discussed based on the experimental results herein. Full article

Crop type classification is critical for crop production estimation and optimal water allocation. Crop type data are challenging to generate if crop reference data are lacking, especially for target years with reference data missed in collection. Is it possible to transfer a trained crop type classification model to retrace the historical spatial distribution of crop types? Taking the Hetao Irrigation District (HID) in China as the study area, this study first designed a 10 m crop type classification framework based on the Google Earth Engine (GEE) for crop type mapping in the current season. Then, its interannual transferability to accurately retrace historical crop distributions was tested. The framework used Sentinel-1/2 data as the satellite data source, combined percentile, and monthly composite approaches to generate classification metrics and employed a random forest classifier with 300 trees for crop classification. Based on the proposed framework, this study first developed a 10 m crop type map of the HID for 2020 with an overall accuracy (OA) of 0.89 and then obtained a 10 m crop type map of the HID for 2019 with an OA of 0.92 by transferring the trained model for 2020 without crop reference samples. The results indicated that the designed framework could effectively identify HID crop types and have good transferability to obtain historical crop type data with acceptable accuracy. Our results found that SWIR1, Green, and Red Edge2 were the top three reflectance bands for crop classification. The land surface water index (LSWI), normalized difference water index (NDWI), and enhanced vegetation index (EVI) were the top three vegetation indices for crop classification. April to August was the most suitable time window for crop type classification in the HID. Sentinel-1 information played a positive role in the interannual transfer of the trained model, increasing the OA from 90.73% with Sentinel 2 alone to 91.58% with Sentinel-1 and Sentinel-2 together. Full article

Wireless power transfer (WPT) for electric vehicles is an emerging technology and a future trend. To increase power density, the coupling coefficient of coils can be designed to be large, forming a strongly coupled WPT system, different from the conventional loosely coupled WPT system. In this way, the power density and efficiency of the WPT system can be improved. This paper investigates the dual-side phase-shift control of the strongly coupled series–series compensated WPT systems. The mathematical models based on the conventional first harmonic approximation and differential equations for the dual-side phase-shift control are built and compared. The dual-side phase-shift angle and its impact on the power transfer direction and soft switching are investigated. It is found that synchronous rectification at strong couplings can lead to hard switching because the dual-side phase shift in this case is over 90°. In comparison, a relatively high efficiency and soft switching can be realized when the dual-side phase shift is below 90°. The experimental results have validated the analysis. Full article

Ganoderma lucidum spores (GLS), the mature germ cells ejected from the abaxial side of the pileus, have diverse pharmacological effects. However, the genetic regulation of sporulation in this fungus remains unknown. Here, samples corresponding to the abaxial side of the pileus were collected from strain YW-1 at three sequential developmental stages and were then subjected to a transcriptome assay. We identified 1598 differentially expressed genes (DEGs) and found that the genes related to carbohydrate metabolism were strongly expressed during spore morphogenesis. In particular, genes involved in trehalose and malate synthesis were upregulated, implying the accumulation of specific carbohydrates in mature G. lucidum spores. Furthermore, the expression of genes involved in triterpenoid and ergosterol biosynthesis was high in the young fruiting body but gradually decreased with sporulation. Finally, spore development-related regulatory pathways were explored by analyzing the DNA binding motifs of 24 transcription factors that are considered to participate in the control of sporulation. Our results provide a dataset of dynamic gene expression during sporulation in G. lucidum. They also shed light on genes potentially involved in transcriptional regulation of the meiotic process, metabolism pathways in energy provision, and ganoderic acids and ergosterol biosynthesis. Full article