Search Results (22)

As COVID-19 transitions from pandemic to endemic, our prevention and control policies have shifted from broad, strict community interventions to focusing on the prevention of cluster outbreaks. Currently, information on the characteristics of cluster outbreaks remains limited. This study describes the features of COVID-19 clusters in Shanghai. It aims to provide valuable insights for managing localized outbreaks. We conducted a retrospective analysis of clusters of confirmed COVID-19 cases. Epidemiological descriptions, the transmission characteristics of clusters, and individual risk factors for contagiousness were analyzed. A total of 381 cases of COVID-19 were confirmed and 67 clusters were identified. Most clusters (58.21%, 39/67) only had two cases, with a declining proportion held by clusters of more cases. Familial transmission was predominant, accounting for 79.10% (53/67) of clusters. Although other types of cluster outbreaks, such as those in workplaces (1.49%, 1/67), occur less frequently compared to household clusters, they tend to involve larger scales and more cases. Workplaces and similar venues are more likely to experience large-scale cluster outbreaks. Contagiousness was higher among cases with runny nose (risk ratio [RR]: 4.8, 95% CI: 1.40–16.44, p-value = 0.01) and those with diabetes (RR: 3.8, 95% CI: 1.01–14.60, p-value = 0.05). In conclusion, household cluster outbreaks, in particular, are both a key priority and a foundational issue. Establishing an indicator system based on the transmissibility of cases holds significant practical value for infectious disease prevention and control. By enhancing household hygiene and developing a case classification and management system based on transmissibility, it is possible to better prevent and control regional COVID-19 outbreaks. Full article

The piggyBac+TET-on transposon induction system has a high efficiency in integrating exogenous genes in multiple cell types, can precisely integrate to reduce genomic damage, has a flexible gene expression regulation, and a strong genetic stability. When used in conjunction with somatic cell nuclear transfer experiments, it can precisely and effectively reveal the intrinsic mechanisms of early biological development. This study successfully reprogrammed black-boned sheep fibroblasts (SFs) into induced pluripotent stem cells (iPSCs) using the piggyBac+TET-on transposon system and investigated their impact on early embryonic development. Seven exogenous reprogramming factors (bovine OCT4, SOX2, KLF4, cMyc, porcine NANOG, Lin-28, and SV40 Large T) were delivered into SFs, successfully inducing iPSCs. A growth performance analysis revealed that iPSC clones exhibited a raised or flat morphology with clear edges, positive alkaline phosphatase staining, and normal karyotypes. The transcriptome analysis indicated a significant enrichment of iPSCs in oxidative phosphorylation and cell proliferation pathways, with an up-regulated expression of the ATP5B, SDHB, Bcl-2, CDK1, and Cyclin D1 genes and a down-regulated expression of BAX (p < 0.05). Somatic cell nuclear transfer experiments demonstrated that the cleavage rate (85% ± 2.12) and blastocyst rate (52% ± 2.11) of the iPSCs were significantly higher than those of the SFs (p < 0.05). The detection of trilineage marker genes confirmed that the expression levels of endoderm (DCN, NANOS3, FOXA2, FOXD3, SOX17), mesoderm (KDR, CD34, NFH), and ectoderm (NEUROD) markers in iPSCs were significantly higher than in SFs (p < 0.01). The findings demonstrate that black-boned sheep iPSCs possess pluripotency and the potential to differentiate into all three germ layers, revealing the mechanisms by which reprogrammed iPSCs influence early embryonic development and providing a critical foundation for research on sheep pluripotent stem cells. Full article

This study designed three sgRNAs (sgRNA139, sgRNA128, and sgRNA109) targeting the prolactin gene receptor (PRLR) in fetal cattle, utilized Cas9 to cleave endogenous DNA, and screened stable cell lines for somatic cell nuclear transfer experiments to investigate the impact of different editing sites on embryonic development. The results showed that sgRNA139 had the highest cleavage efficiency (Fcut = 0.65, Indels = 42.19%), while sgRNA109 had the lowest (Fcut = 0.45, Indels = 35.31%). No significant differences were observed in cell growth status after electroporation (p > 0.05), and the transfection efficiency exceeded 90% after five days of culture. In the evaluation of key embryonic development indicators, sgRNA109 significantly reduced the cleavage rate and blastocyst rate (p < 0.01), whereas sgRNA139 showed no significant effect on the cleavage rate (p > 0.05), but its blastocyst rate was slightly lower than that of the control group (p > 0.05). This study demonstrates that highly specific sgRNAs and stable edited cell lines used as donor cells can significantly regulate the later stages of embryonic development. This study not only provides new experimental evidence for the functional study of the PRLR but also lays an important theoretical foundation for the innovation of molecular breeding technologies in dairy cattle. Full article

The Rural Revitalization Strategy is an important policy enacted by the Chinese government, aimed at bridging the disparity between urban and rural areas while enhancing economic, cultural, and social development levels in rural areas. Research evaluating return-to-hometown entrepreneurship policies is pivotal for facilitating the sustained flow and comprehensive sustainable development of capital, technology, and talent to rural areas within the Rural Revitalization Strategy framework. This study employs NVivo12 and the policy modeling consistency (PMC) index to analyze trends, characteristics, and policy tool utilization quantitatively, as well as regional differences or commonalities in 55 return-to-hometown entrepreneurship policies of 16 provinces and autonomous regions in China from 2013 to 2023. The content analysis of these policy texts reveals trends characterized by multistakeholder participation, an increasing emphasis on technology integration, and increased efforts toward talent retention. Overall, the results of the PMC model analysis indicate that the scores for return-to-hometown entrepreneurship policies across 16 provinces (municipalities and autonomous regions) are relatively high in terms of the indices of policy field, policy content, and policy tools, whereas the scores are comparatively low in terms of the indices of policy nature and policy timeliness. The remaining indices present moderate scores with slight differences. When examining the average scores between the eastern and central-western regions, it is observed that there is only a very slight difference in the index of policy timeliness and policy implementation. Additionally, the performance of eastern regions is superior in terms of the index of policy issuer, policy functions, and policy content. However, central-western regions demonstrate higher scores in the indices of policy nature, policy field, policy tools, and policy evaluation. Full article

With increasing societal attention being directed to resource and environment problems, the research focus on high reclaimed asphalt content mixtures has become pertinent. The degree of asphalt fusion in the thermal regeneration process of a high RAP content reclaimed asphalt mixture has a great influence on its performance. In order to explore the development process of hot in-place recycling mixture performance along with internal asphalt fusion, this study conducted research on a geothermal regeneration mixture with 80% RAP content. Dynamic shear rheology (DSR), infrared spectroscopy, and scanning electron microscopy were used to investigate the fusion of recycled mixture under different placement times (1 day, 4 days, and 7 days), and the road performance and fatigue life of the recycled mixture under different placement times were then studied. The results showed that the fusion degree of old asphalt and new asphalt in a recycled asphalt mixture reached 100%, and gradually increased with the extension of placement time. With the increase in placement time, the high temperature performance of the regenerated mixture gradually decreased, the water stability gradually increased, and the low-temperature performance and fatigue life significantly increased from 1 day to 7 days, by 19% and 32%, respectively. Full article

An advanced strategy was developed for the synthesis of molecular sieve-supported Pd catalysts. In this method, reductant containing C=C was in-situ prepared and pre-dispersed in the pore of the zeolites. The C=C group in the reductant can reduce the Pd²⁺ to Pd⁰ efficiently, leading to the formation of small and uniform Pd nanoparticles (~2 nm). The physical and chemical properties of the catalyst were characterized by XRD, TEM, XPS (ICP-OES), N₂ isothermal adsorption-desorption, and H₂-TPR. These catalysts showed high catalytic performance for the hydrogenation of nitrobenzene to aniline. All the TOFs for 1.5 Pd/Y, 1.5 Pd/ZSM-5, and 1.5 Pd/MOR with 1.5 wt% Pd loading are higher than 1000 h⁻¹ at 30 °C and 0.1 MPa H₂. Meanwhile, kinetic analysis for 2.0 Pd/Y was carried out, and an apparent activation energy of 28.88 kJ mol⁻¹ was obtained, which is lower than most of the reported values in the literature. Furthermore, these catalysts were stable and recyclable. Full article

The catalytic transformation of biomass-based furan compounds (furfural and HMF) for the synthesis of organic chemicals is one of the important ways to utilize renewable biomass resources. Among the numerous high-value products, cyclopentanone derivatives are a kind of valuable compound obtained by the hydrogenation rearrangement of furfural and HMF in the aqueous phase of metal–hydrogen catalysis. Following the vast application of cyclopentanone derivatives, this reaction has attracted wide attention since its discovery, and a large number of catalytic systems have been reported to be effective in this transformation. Among them, the design and synthesis of metal catalysts are at the core of the reaction. This review briefly introduces the application of cyclopentanone derivatives, the transformation mechanism, and the pathway of biomass-based furan compounds for the synthesis of cyclopentanone derivatives. The important progress of metal catalysts in the reaction since the first report in 2012 up to now is emphasized, the characteristics and catalytic performance of different metal catalysts are introduced, and the critical role of metal catalysts in the reaction is discussed. Finally, the future development of this transformation process was prospected. Full article

When using ultrasound to detect the thickness of protective coatings on assembled steel structures, the coatings are extremely thin, which can cause echo signals to overlap and impair the detection accuracy. Therefore, the study of the separation of the superimposed signals is essential for the precise measurement of the thickness of thinner coatings. A method for signal time domain feature extraction based on an adaptive feature dictionary and K–SVD is investigated. First, the wavelet transform, which is sensitive to singular signal values, is used to identify the extreme values of the signal and use them as the new signal to be processed. Then, the feature signal extracted by wavelet transform is transformed into Hankel matrix form, and the initial feature dictionary is constructed by period segmentation and random extraction. The optimized feature dictionary is subsequently obtained by enhancing the K–SVD algorithm. Finally, the time domain signal is reconstructed using the optimized feature dictionary. Simulations and experiments demonstrate that the method is more accurate in separating mixed signals and extracting signal time domain feature information than the conventional wavelet transform and Gabor dictionary-based MP algorithm, and that it is more advantageous in detecting the thickness of protective coatings. Full article

Recently, scientists have been facing major obstacles in terms of improving the performances of dielectric materials for triboelectric nanogenerators. The triboelectric nanogenerator (TENG) is one of the first green energy technologies that can convert random mechanical kinetic energy into electricity. The surface charge density of TENGs is a critical factor speeding up their commercialization, so it is important to explore unique methods to increase the surface charge density. The key to obtaining a high-performance TENG is the preparation of dielectric materials with good mechanical properties, thermal stability and output performance. To solve the problem of the low output performance of PI-based triboelectric nanogenerators, we modified PI films by introducing nanomaterials and designed a new type of sandwich-shaped nanocomposite film. Herein, we used polyimide (PI) with ideal mechanical properties, excellent heat resistance and flexibility as the dielectric material, prepared an A-B-A sandwich structure with PI in the outer layer and modified a copper calcium titanate/polyimide (CCTO/PI) storage layer in the middle to improve the output of a TENG electrode. The doping amount of the CCTO was tailored. The results showed that at 8 wt% CCTO content, the electrical output performance was the highest, and the open-circuit voltage of CCTO/PI was 42 V. In the TENG, the open-circuit voltage, short-circuit current and transfer charge of the prepared sandwich-structured film were increased by 607%, 629% and 672% compared to the TENG with the PI thin film, respectively. This study presents a novel strategy of optimizing dielectric materials for triboelectric nano-generators and has great potential for the future development of high output-performance TENGs. Full article

The widespread use of cross-linked polyethylene (XLPE) as insulation in cables may be attributed to its outstanding mechanical and dielectric properties. In order to quantitatively evaluate the insulation status of XLPE after thermal ageing, an accelerated thermal ageing experimental platform is established. Polarization and depolarization current (PDC) as well as elongation at break of XLPE insulation under different ageing durations are measured. XLPE insulation state is determined by the elongation at break retention rate (ER%). Based on the extended Debye model, the paper proposed the stable relaxation charge quantity and the dissipation factor at 0.1 Hz to evaluate the insulation state of XLPE. The results show that the ER% of XLPE insulation decreases with the growth of ageing degree. The polarization and depolarization current of XLPE insulation will increase obviously with thermal ageing. Conductivity and trap level density will also increase. The number of branches of the extended Debye model increases, and new polarization types appear. Stable relaxation charge quantity and dissipation factor at 0.1 Hz proposed in this paper have a good fitting relationship with ER% of XLPE insulation, which can evaluate the thermal ageing state of XLPE insulation effectively. Full article

Evapotranspiration (ET) estimations at high spatiotemporal resolutions in urban areas are crucial for extreme weather forecasting and water management. However, urban ET estimation remains a major challenge in current urban hydrology and regional climate research due to highly heterogeneous environments, human interference, and a lack of observations. In this study, an urban ET model, called the PT-Urban model, was proposed for half-hourly ET estimations at a 10 m resolution. The PT-Urban model was validated using observations from the Hotel Torni urban flux site during the 2018 growing season. The results showed that the PT-Urban model performed satisfactorily, with an R² and root-mean-square error of 0.59 and 14.67 W m⁻², respectively. Further analysis demonstrated that urban canopy heat storage and shading effects are essential for the half-hourly urban energy balance. Ignoring the shading effects led to a 38.7% urban ET overestimation. Modeling experiments further proved that flux footprint variations were critical for the accurate estimation of urban ET. The setting source areas either as an invariant 70% historical footprint or as a circle with a 1 km radius both resulted in poor performances. This study presents a practical method for the accurate estimation of urban ET with high spatiotemporal resolution and highlights the importance of real-time footprints in urban ET estimations. Full article

The problems of environmental pollution are increasingly severe. Among them, industrial wastewater is one of the primary sources of pollution, so it is essential to deal with wastewater, especially oil and water mixtures. At present, biomimetic materials with special wettability have been proven to be effective in oil-water separation. Compared with three-dimensional (3D) materials, two-dimensional (2D) materials show unique advantages in the preparation of special wettable materials due to their high specific surface area, high porosity, controlled structure, and rich functional group rich on the surface. In this review, we first introduce oil–water mixtures and the common oil–water separation mechanism. Then, the research progress of 2D materials in oil–water separation is presented, including but not limited to their structure, types, preparation principles, and methods. In addition, it is still impossible to prepare 2D materials with large sizes because they are powder-like, which greatly limits the application in oil–water separation. Therefore, we provide here a review of several ways to transform 2D materials into 3D materials. In the end, the challenges encountered by 2D materials in separating oil–water are also clarified to promote future applications. Full article

With industry development, the separation of oily wastewater is becoming more critical. Inspired by organisms such as lotus leaves, biomimetic superhydrophobic surfaces with micro-nano structures have shown great potential in this regard. In this work, PDMS/PVDF oil–water separation membranes with designed microstructures were prepared by electrospinning technology. The membrane-forming effect of electrospinning with different ratios of PDMS and PVDF was studied. The study found that membranes with high PDMS content were more likely to form microspheres, and PDMS tended to concentrate on the microspheres. The results also showed that the microspheres would bring better hydrophobicity to the membrane. When the ratio of PDMS to PVDF is 1:2, the membrane has a water contact angle of up to 150° and an oil contact angle of 0°. At this ratio, the separation efficiency of the membrane for the water-in-oil emulsion is 98.7%, and it can still maintain more than 98% after ten separation cycles, which is a good candidate for oil–water separation. Furthermore, microspheres enable the membrane to achieve macroscopic uniformity and microscopic phase separation so that the membranes have both good elongation and fracture strength. In addition, the PDMS/PVDF membranes also exhibit excellent UV resistance, and their UV protection factor is greater than 185, making them a potential UV protective material. Full article

The majority of proteins are subjected to post-translational modifications (PTMs), regardless of whether they occur in or after biosynthesis of the protein. Capable of altering the physical and chemical properties and functions of proteins, PTMs are thus crucial. By fostering the proliferation, migration, and invasion of cancer cells with which they communicate in the tumor microenvironment (TME), M2 macrophages have emerged as key cellular players in the TME. Furthermore, growing evidence illustrates that PTMs can occur in M2 macrophages as well, possibly participating in molding the multifaceted characteristics and physiological behaviors in the TME. Hence, there is a need to review the PTMs that have been reported to occur within M2 macrophages. Although there are several reviews available regarding the roles of M2 macrophages, the majority of these reviews overlooked PTMs occurring within M2 macrophages. Considering this, in this review, we provide a review focusing on the advancement of PTMs that have been reported to take place within M2 macrophages, mainly in the TME, to better understand the performance of M2 macrophages in the tumor microenvironment. Incidentally, we also briefly cover the advances in developing inhibitors that target PTMs and the application of artificial intelligence (AI) in the prediction and analysis of PTMs at the end of the review. Full article

In this paper, a two-dimensional axisymmetric module of gas arc extinguishing was simulated using energy balance theories. We used simulation to study the energy distribution change during the gas arc-extinguishing process. We built a lightning impulse current experimental platform according to the IEC standard, and experiments verified the preliminary conclusions of the simulation. Comparison curves of the experimental data and simulation calculations were drawn in the range of 20 kV to 70 kV. Simulation and experimental results showed that the arc-extinguishing ability of long-gap gas arcs is negatively correlated with voltage level and positively correlated with distance. Furthermore, within the allowable range of conditions, increasing the length of the chamber rather than shortening it helps to extinguish the arc more effectively and quickly. Full article