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Search Results (7)

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Authors = Patrick Chaimbault ORCID = 0000-0001-7588-8751

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14 pages, 712 KiB  
Article
Daniellia oliveri (Rolfe) Hutch and Dalziel: Antimicrobial Activities, Cytotoxicity Evaluation, and Phytochemical Identification by GC-MS
by Nassifatou Koko Tittikpina, Gilbert Kirsch, Raphaël Emmanuel Duval, Patrick Chaimbault and Claus Jacob
Antibiotics 2022, 11(12), 1699; https://doi.org/10.3390/antibiotics11121699 - 25 Nov 2022
Cited by 7 | Viewed by 2503
Abstract
During a previous study that identified plants used in traditional medicine in Togo to treat infectious diseases, Daniellia oliveri was specifically reported to treat intertrigo and candidiasis. Consequently, to explore the anti-infective potential of this plant, we investigated the antibacterial and the antifungal [...] Read more.
During a previous study that identified plants used in traditional medicine in Togo to treat infectious diseases, Daniellia oliveri was specifically reported to treat intertrigo and candidiasis. Consequently, to explore the anti-infective potential of this plant, we investigated the antibacterial and the antifungal activity of the plant’s parts, as well as the cytotoxic activities of raw extracts and subsequent fractions, and the chemical composition of the most active fractions. In order to evaluate the antimicrobial activity, MICs were determined using the broth dilution method. Then, the most active fractions were evaluated for cytotoxicity by using normal human cells (MRC-5 cells) via the MTT assay. Finally, the most active and not toxic fractions were phytochemically investigated by GC-MS. Interestingly, all the raw extracts and fractions were active against the bacteria tested, with MICs ranging from 16 µg/mL to 256 µg/mL, while no antifungal activity was observed at 256 µg/mL, the highest tested concentration. Moreover, no toxicity was observed with most of the active fractions. The subsequent chemical investigation of the most interesting fractions led to identifying terpenes, phytosterols, phenolic compounds, and fatty acids as the main compounds. In conclusion, this study demonstrated that D. oliveri possesses valuable antibacterial activities in accordance with traditional use. Full article
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13 pages, 4119 KiB  
Article
Elicitation of Antimicrobial Active Compounds by Streptomyces-Fungus Co-Cultures
by Matthieu Nicault, Ali Zaiter, Stéphane Dumarcay, Patrick Chaimbault, Eric Gelhaye, Pierre Leblond and Cyril Bontemps
Microorganisms 2021, 9(1), 178; https://doi.org/10.3390/microorganisms9010178 - 15 Jan 2021
Cited by 25 | Viewed by 5032
Abstract
The bacteria of the genus Streptomyces and Basidiomycete fungi harbor many biosynthetic gene clusters (BGCs) that are at the origin of many bioactive molecules with medical or industrial interests. Nevertheless, most BGCs do not express in standard lab growth conditions, preventing the full [...] Read more.
The bacteria of the genus Streptomyces and Basidiomycete fungi harbor many biosynthetic gene clusters (BGCs) that are at the origin of many bioactive molecules with medical or industrial interests. Nevertheless, most BGCs do not express in standard lab growth conditions, preventing the full metabolic potential of these organisms from being exploited. Because it generates biotic cues encountered during natural growth conditions, co-culture is a means to elicit such cryptic compounds. In this study, we explored 72 different Streptomyces-fungus interaction zones (SFIZs) generated during the co-culture of eight Streptomyces and nine fungi. Two SFIZs were selected because they showed an elicitation of anti-bacterial activity compared to mono-cultures. The study of these SFIZs showed that co-culture had a strong impact on the metabolic expression of each partner and enabled the expression of specific compounds. These results show that mimicking the biotic interactions present in this ecological niche is a promising avenue of research to explore the metabolic capacities of Streptomyces and fungi. Full article
(This article belongs to the Special Issue Natural Products from Streptomyces)
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4 pages, 526 KiB  
Abstract
Diagnosis of Biological Activities by Mass Spectrometry
by Vincent Carré, Pierre Leroy and Patrick Chaimbault
Proceedings 2019, 11(1), 36; https://doi.org/10.3390/proceedings2019011036 - 29 Apr 2019
Viewed by 1289
Abstract
Oxidative reactions are vital but also cause important stresses and cellular damages resulting in cancers, cardiovascular or neurodegenerative diseases. Antioxidant secondary metabolites from plant can be mobilized for the cell defense and their main source is precisely the food intake such as vegetables, [...] Read more.
Oxidative reactions are vital but also cause important stresses and cellular damages resulting in cancers, cardiovascular or neurodegenerative diseases. Antioxidant secondary metabolites from plant can be mobilized for the cell defense and their main source is precisely the food intake such as vegetables, fruits or beverages. Screening natural active metabolites in plants requires different analytical techniques among which mass spectrometry became one of the most popular, not just because of its ability to provide structural information on involved molecules but also because this technique belongs to the arsenal of diagnostic tool for the determination of biological activities. Full article
(This article belongs to the Proceedings of CA16112 - Luxemburg 2019)
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13 pages, 988 KiB  
Review
Natural and Synthetic Coumarins with Effects on Inflammation
by Gilbert Kirsch, Ahmed Bakr Abdelwahab and Patrick Chaimbault
Molecules 2016, 21(10), 1322; https://doi.org/10.3390/molecules21101322 - 2 Oct 2016
Cited by 110 | Viewed by 10019
Abstract
In this review, we will present the different aspects of coumarins and derivatives, from natural origins or synthetically prepared, and their action on inflammation. Coumarins and also furo- and pyranocoumarins are found in many different plants. These compounds are very often investigated for [...] Read more.
In this review, we will present the different aspects of coumarins and derivatives, from natural origins or synthetically prepared, and their action on inflammation. Coumarins and also furo- and pyranocoumarins are found in many different plants. These compounds are very often investigated for antioxidant properties. Other biological properties are also possible and anti-inflammation activity is one of these. As coumarins are also available quite easily via synthesis, natural ones can be prepared this way but derivatives with special substituents are also feasible. A review on the same topic appeared in 2004 and our contribution will take into account everything published since then. Full article
(This article belongs to the Special Issue Natural Products and Inflammation)
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12 pages, 7354 KiB  
Communication
Turning Waste into Value: Nanosized Natural Plant Materials of Solanum incanum L. and Pterocarpus erinaceus Poir with Promising Antimicrobial Activities
by Sharoon Griffin, Nassifatou Koko Tittikpina, Adel Al-marby, Reem Alkhayer, Polina Denezhkin, Karolina Witek, Koffi Apeti Gbogbo, Komlan Batawila, Raphaël Emmanuel Duval, Muhammad Jawad Nasim, Nasser A. Awadh-Ali, Gilbert Kirsch, Patrick Chaimbault, Karl-Herbert Schäfer, Cornelia M. Keck, Jadwiga Handzlik and Claus Jacob
Pharmaceutics 2016, 8(2), 11; https://doi.org/10.3390/pharmaceutics8020011 - 19 Apr 2016
Cited by 22 | Viewed by 7396
Abstract
Numerous plants are known to exhibit considerable biological activities in the fields of medicine and agriculture, yet access to their active ingredients is often complicated, cumbersome and expensive. As a consequence, many plants harbouring potential drugs or green phyto-protectants go largely unnoticed, especially [...] Read more.
Numerous plants are known to exhibit considerable biological activities in the fields of medicine and agriculture, yet access to their active ingredients is often complicated, cumbersome and expensive. As a consequence, many plants harbouring potential drugs or green phyto-protectants go largely unnoticed, especially in poorer countries which, at the same time, are in desperate need of antimicrobial agents. As in the case of plants such as the Jericho tomato, Solanum incanum, and the common African tree Pterocarpus erinaceus, nanosizing of original plant materials may provide an interesting alternative to extensive extraction and isolation procedures. Indeed, it is straightforward to obtain considerable amounts of such common, often weed-like plants, and to mill the dried material to more or less uniform particles of microscopic and nanoscopic size. These particles exhibit activity against Steinernema feltiae or Escherichia coli, which is comparable to the ones seen for processed extracts of the same, respective plants. As S. feltiae is used as a model nematode indicative of possible phyto-protective uses in the agricultural arena, these findings also showcase the potential of nanosizing of crude “waste” plant materials for specific practical applications, especially—but not exclusively—in developing countries lacking a more sophisticated industrial infrastructure. Full article
(This article belongs to the Special Issue Nanocrystals)
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22 pages, 1044 KiB  
Article
New Peptide-Conjugated Chlorin-Type Photosensitizer Targeting Neuropilin-1 for Anti-Vascular Targeted Photodynamic Therapy
by Ezatul Ezleen Kamarulzaman, Amirah Mohd Gazzali, Samir Acherar, Céline Frochot, Muriel Barberi-Heyob, Cédric Boura, Patrick Chaimbault, Estelle Sibille, Habibah A. Wahab and Régis Vanderesse
Int. J. Mol. Sci. 2015, 16(10), 24059-24080; https://doi.org/10.3390/ijms161024059 - 12 Oct 2015
Cited by 32 | Viewed by 7781
Abstract
Photodynamic therapy (PDT) is a cancer treatment modality that requires three components, namely light, dioxygen and a photosensitizing agent. After light excitation, the photosensitizer (PS) in its excited state transfers its energy to oxygen, which leads to photooxidation reactions. In order to improve [...] Read more.
Photodynamic therapy (PDT) is a cancer treatment modality that requires three components, namely light, dioxygen and a photosensitizing agent. After light excitation, the photosensitizer (PS) in its excited state transfers its energy to oxygen, which leads to photooxidation reactions. In order to improve the selectivity of the treatment, research has focused on the design of PS covalently attached to a tumor-targeting moiety. In this paper, we describe the synthesis and the physico-chemical and photophysical properties of six new peptide-conjugated photosensitizers designed for targeting the neuropilin-1 (NRP-1) receptor. We chose a TPC (5-(4-carboxyphenyl)-10,15, 20-triphenyl chlorine as photosensitizer, coupled via three different spacers (aminohexanoic acid, 1-amino-3,6-dioxaoctanoic acid, and 1-amino-9-aza-3,6,12,15-tetraoxa-10-on-heptadecanoic acid) to two different peptides (DKPPR and TKPRR). The affinity towards the NRP-1 receptor of the conjugated chlorins was evaluated along with in vitro and in vivo stability levels. The tissue concentration of the TPC-conjugates in animal model shows good distribution, especially for the DKPPR conjugates. The novel peptide–PS conjugates proposed in this study were proven to have potential to be further developed as future NRP-1 targeting photodynamic therapy agent. Full article
(This article belongs to the Special Issue Advances in Photodynamic Therapy)
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14 pages, 739 KiB  
Article
MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves
by Loïc Becker, Vincent Carré, Anne Poutaraud, Didier Merdinoglu and Patrick Chaimbault
Molecules 2014, 19(7), 10587-10600; https://doi.org/10.3390/molecules190710587 - 21 Jul 2014
Cited by 48 | Viewed by 15393
Abstract
To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic acid (DHB) was found [...] Read more.
To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic acid (DHB) was found to be the most efficient matrix allowing a broader range of detected stilbene phytoalexins. Resveratrol, but also more toxic compounds against fungi such as pterostilbene and viniferins, were identified and mapped. Their spatial distributions on grapevine leaves irradiated by UV show their specific colocation around the veins. Moreover, MALDI MSI reveals that resveratrol (and piceids) and viniferins are not specifically located on the same area when leaves are infected by Plasmopara viticola. Results obtained by MALDI mass spectrometry imaging demonstrate that this technique would be essential to improve the level of knowledge concerning the role of the stilbene phytoalexins involved in a stress event. Full article
(This article belongs to the Special Issue Phytoalexins: Current Progress and Future Prospects)
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