Search Results (128)

Reducing structural mass and volume is critical to improving efficiency and payload capacity in next-generation small satellites and CubeSats. Additive manufacturing, particularly material extrusion, offers design flexibility and enables the production of lightweight, functional metallic components. This study investigates the integration of nickel–titanium shape memory alloy wires into aluminum-based matrices using a sinter-based material extrusion process, aiming to develop compact actuator systems for aerospace applications. A customized AlSi7Mg aluminum alloy feedstock was extruded into filament form, printed, and embedded with shape memory alloy wires, allowing consolidation during sintering. X-ray micro-computed tomography was used to analyze internal defects and matrix–wire interfacial contact, before and after sintering. Tensile testing of the embedded actuator structures revealed effective mechanical bonding and actuation behavior. The results demonstrate that controlled shrinkage and interfacial bonding enable reliable embedding of shape memory elements without compromising structural integrity. This work provides a promising framework for developing multifunctional aerospace components, where active actuation and structural efficiency can be combined through advanced material extrusion-based manufacturing. Full article

Infections with the Hepatitis B (HBV) and Hepatitis C (HCV) viruses share some transmission routes, which is why coinfection with these viruses becomes common, especially in endemic areas. This study evaluated the immunological response profile, viral load, and liver damage in groups monoinfected with HBV or HCV and in those co-infected with HBV/HCV. The groups were composed of 22 patients monoinfected by HCV, 22 patients monoinfected by HBV, and 34 co-infected by HBV/HCV, according to serological markers and molecular biology tests. The study was carried out from December 2017 to October 2019. Virus detection employed enzyme immunoassay, Enzyme-Linked Immunosorbent Assay (ELISA), and real-time PCR, while liver function and fibrosis were assessed using biochemical tests and Fibroscan. To research the immunological profile, cytokines were quantified using the BIO-Plex Pro Human Cytokine. Comparing the groups, both mono- and co-infected patients exhibited a Th1 immune response profile. HCV monoinfection notably showed significantly elevated serum levels of INF-γ (p < 0.01) and TNF-α (p < 0.01). The viral load was significantly higher in the HCV monoinfected group when compared to the other groups. Regarding liver damage, patients with a high level of fibrosis (F4) presented significant levels of cytokines INF-γ (p < 0.001), IL-17 (p < 0.0001), and TNF-α (p < 0.0001). Full article

Piroplasmids (Babesia spp., Rangelia spp., Theileria spp., Cytauxzoon spp.) are tick-borne apicomplexan protozoa that infect, depending on the species, erythrocytes and leucocytes in a wide variety of mammals and birds. The genera Bartonella and Borrelia include vector-borne bacteria that can infect and cause disease in both animals and humans. Detection of hemotropic bacteria and piroplasmids in wild animals is often challenging due to low bacteremia or parasitemia. Digital (d)PCR has proven to be an effective modality for the detection and quantification of DNA of hemotropic pathogens with low parasitemia. This study compared dPCR results from 366 biological samples from seven different Brazilian wild animal groups (5 Xenarthra species, 5 deer species, 3 felid species, 1 canid species, 3 rodent species, 1 bat species, 1 tapir species, and 12 bird species) to two other molecular diagnostic techniques: quantitative real-time (qPCR) and nested (nPCR). For this study, DNA extracted from wild animal blood and spleen samples were subjected to a multiplex dPCR assay for piroplasmids, Bartonella spp., and Borrelia spp. For comparison, the same primers and probes for each agent were used in qPCR assays. Additionally, an nPCR based on the 18S rRNA gene for piroplasmids was performed. The proportions of positive results obtained using dPCR were 85.5% for piroplasmids, 33.6% for Bartonella spp., and 16.7% for Borrelia spp. For all tested agents, dPCR proved to be the technique with the highest sensitivity, making it a useful tool for screening vector-borne agents in biological samples from wild animals with low parasitemia. Full article

Background/Objective: Urinary tract infections (UTIs) are a significant public health concern worldwide, yet longitudinal data from Portuguese hospital settings remain limited. This study aimed to characterize epidemiological trends, microbial etiology, antimicrobial resistance patterns, and associated risk factors of UTIs over a five-year period (2018–2022) in a central Portuguese hospital. Methods: In this retrospective observational study, 23,682 positive urine cultures were analyzed from specimens collected between January 2018 and December 2022. Data were extracted from the laboratory information system and included patient demographics, clinical service of origin, isolated microorganisms, resistance profiles, and annual antibiotic consumption (Defined Daily Dose (DDD) per 1000 patient-days). UTI prevalence was calculated as the proportion of positive cultures among all urine samples processed annually. Results: The positivity rate increased from 18.7% in 2018 to 22.7% in 2022, with a peak in 2019. Women represented around 70% of cases throughout the study period. Most infections originated from inpatient wards, followed by emergency services. Escherichia coli remained the leading pathogen (≈62%), followed by Klebsiella pneumoniae (≈14%) and Enterococcus faecalis (≈8%). Risk factors included catheterization (37.2%), prior UTI history (22.1%), and diabetes mellitus (18.5%). Longer hospital stays (>7 days) were associated with increased positivity. For E. coli, resistance ranged from 2% (amikacin) to 41% (ampicillin), with increasing resistance to ertapenem and fosfomycin and decreasing resistance to several key antibiotics. K. pneumoniae showed 4–36% resistance across antimicrobials, with notable increases for fosfomycin, meropenem, and cefuroxime axetil. Antibiotic usage trends reflected these patterns, with declining use of amikacin and rising use of cefuroxime axetil and meropenem. Conclusions: Over the five-year period, both UTI prevalence and resistance to critical antimicrobials increased, reinforcing the need to update empirical treatment guidelines. Identified risk factors may inform targeted prevention strategies. Ongoing surveillance and antimicrobial stewardship are crucial to mitigate the rising burden of UTIs and resistance Full article

Incorporating solid waste into polymeric matrices has proven effective in developing composites with enhanced mechanical and thermal properties. This study investigates a composite based on recycled high-density polyethylene (HDPE), reinforced with fine charcoal particles, assessing its thermal, microstructural, and density properties. Two processing methods (compression molding and extrusion) and four charcoal concentrations (0%, 5%, 10%, and 15 wt%) were evaluated. Thermal characterization was performed using thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FTIR). The microstructure was analyzed through scanning electron microscopy (SEM) and X-ray diffraction (XRD), while the density was determined via X-ray densitometry. SEM revealed increased porosity with charcoal addition. The thermal properties and crystallinity of the composites were not significantly affected by variations in the manufacturing method or charcoal concentration. FTIR analysis identified characteristic peaks, while TGA indicated mass loss between 400 and 500 °C, with a maximum decomposition temperature of 487 °C. XRD confirmed the semicrystalline structure typical of HDPE. Thus, incorporating charcoal residues can reduce the use of fossil-based materials while providing a sustainable application for industrial waste. Full article

Background: Lung cancer (LC) is the leading cause of cancer-related deaths worldwide. Effective screening strategies for early diagnosis that could improve disease prognosis are lacking. Non-invasive breath analysis of volatile organic compounds (VOC) is a potential method for earlier LC detection. This study explores the association of VOC profiles with artificial intelligence (AI) to achieve a sensitive, specific, and fast method for LC detection. Patients and methods: Exhaled breath air samples were collected from 123 healthy individuals and 73 LC patients at two clinical sites. The enrolled patients had LC diagnosed with different stages. Breath samples were collected before undergoing any treatment, including surgery, and analyzed using gas chromatography coupled to ion-mobility spectrometry (GC-IMS). AI methods classified the overall chromatographic profiles. Results: GC-IMS is highly sensitive, yielding detailed chromatographic profiles. AI methods ranked the sets of exhaled breath profiles across both groups through training and validation steps, while qualitative information was deliberately not taking part nor influencing the results. The K-nearest neighbor (KNN) algorithm classified the groups with an accuracy of 90% (sensitivity = 87%, specificity = 92%). Narrowing the LC group to those only in early-stage IA, the accuracy was 90% (sensitivity = 90%, specificity = 93%). Conclusions: Evaluation of the global exhaled breath profiles using AI algorithms enabled LC detection and demonstrated that qualitative information may not be required, thus easing the frustration that many studies have experienced so far. The results show that this approach coupled with screening protocols may improve earlier detection of LC and hence its prognosis. Full article

This overview aimed at assessing botulinum toxin type A (BoNT-A)’s effectiveness in managing bruxism compared to a placebo, the absence of treatment, or other interventions in adults. Only systematic reviews (SRs), with or without a meta-analysis, were included. A comprehensive literature search was conducted on 16 June 2024, encompassing seven databases and grey literature sources. Experts and reference lists of the included SRs were also consulted. Study selection was conducted in two phases by two independent authors. Methodological quality was evaluated using AMSTAR-2. Overlap was assessed using the corrected covered area. The 14 included SRs addressed several outcomes. In most studies, BoNT-A showed effectiveness in reducing pain (n = 10), the frequency of bruxism events (n = 7), and the maximum bite force (n = 5). None of the SRs effectively evaluated BoNT-A’s impact on functional movements. All of the included SRs scored “critically low” and “low” confidence rates in AMSTAR-2. Only one SR attempted to assess the certainty of the evidence, although unsuccessfully. The overlap across included SRs was rated as “high”, with a corrected covered area of 10.11%. The current systematic reviews on BoNT-A for bruxism lack methodological quality, limiting the reliability of their findings. Despite evidence indicating a potential reduction in pain, bruxism events, and the maximum bite force, methodological limitations prevent definitive conclusions from being drawn. High-quality research with standardized methodologies is essential to establish BoNT-A’s efficacy and support evidence-based clinical practice. Full article

Urinary tract infections (UTIs) are among the most prevalent infectious diseases in older women, especially those over 65 years of age. Physiological changes related to aging, comorbidities, and frequent use of medical devices such as urinary catheters increase susceptibility. Increasing antimicrobial resistance further complicates treatment strategies. This study aims to describe the epidemiological profile of UTI in women over 65 years of age, focusing on the characterization of etiological agents, observed antimicrobial resistance patterns, and commonly reported risk factors. We conducted a retrospective analysis of microbiological and clinical data from elderly women diagnosed with UTIs. Bacterial isolates were identified and antimicrobial susceptibility profiles were evaluated over a specified period. A statistical analysis was performed to determine the prevalence of different pathogens and antibiotic resistance trends. Escherichia coli was the predominant uropathogen, consistent across different clinical scenarios and patient conditions. The four most common bacterial strains—E. coli, Klebsiella pneumoniae, Proteus mirabilis, and Enterococcus faecalis—aligned with global epidemiological data. In Escherichia coli a significant increase in resistance to nitrofurantoin was observed, possibly indicating excessive empirical use, while resistance to other antibiotics, such as amoxicillin/clavulanic acid and ertapenem, remained stable or decreased. Institutional antibiotic stewardship programs likely contributed to this trend. The study highlights E. coli as the main etiological agent in elderly women with UTIs. The observed resistance patterns emphasize the need for localized antimicrobial surveillance and personalized therapeutic approaches. Continuous microbiological monitoring and rational use of antibiotics are crucial to optimize treatment outcomes and control the development of resistance. Full article

The lack of personalized wound dressings tailored to individual needs can significantly hinder wound healing. Hydrogels offer a promising solution, as they can be engineered to mimic the extracellular matrix (ECM), providing an optimal environment for wound repair. The integration of digital light processing (DLP), a high-resolution 3D printing process, allows precise customization of hydrogel-based wound dressings. In this study, gelatin methacrylate (GelMA)-based formulations were prepared in combination with three different polymeric precursors: methacrylated hyaluronic acid (HAMA), poly (ethylene glycol) diacrylate (PEGDA) and allyl cellulose (MCCA). These precursors were used to print high-resolution micropatterned patches. The printed constructs revealed a high gel content and a good resistance to hydrolytic degradation. To improve the adhesive and antioxidant properties of the printed patches, gallic acid (GA) was incorporated through surface functionalization. This enabled the scavenging of approximately 80% of free radicals within just 4 h. The adhesive properties of the printed wound dressings were also significantly improved, with further enhancement observed upon the addition of Fe³⁺ ions. In vitro cytocompatibility tests using a fibroblast (NHDF) cell line confirmed the suitability of the materials for biomedical applications. Thus, this study demonstrates the potential of DLP-printed hydrogels as advanced personalized wound dressing materials. Full article

Non-biodegradable superabsorbent polymers (SAPs) in personal care products (PCPs) pose significant environmental and health concerns despite their high absorption capacity. The aim of this study was to develop cellulose-based hydrogels as a sustainable alternative to those conventional SAPs, taking advantage of cellulose properties such as biocompatibility, biodegradability, and hydrophilicity. A synthesized allyl cellulose (AC) derivative was copolymerized with unusual monomers used in the production of SAPs, and the influence of monomer ratios, crosslinking density, and the ratio of cellulose to monomers on the absorption capacity was investigated and optimized. The most promising hydrogels were fully characterized for the proposed application and compared with a commercial SAP extracted from a baby diaper. The cellulose-based hydrogels showed promising absorption capacities in synthetic urine (~15 g/g), and a high centrifuge retention capacity (12.5 g/g), which was only slightly lower than the commercial SAP. These new hydrogels exhibited excellent biocompatibility and outperformed the established commercial diaper SAP. This study represents a more sustainable alternative to conventional SAPs, potentially reducing health risks while increasing the bio-based content of PCPs. Further optimization of these hydrogels could transform the hygiene product industry, by providing a balance between performance and environmental sustainability. Full article

Background: Gaseous elemental mercury (Hg⁰ or GEM) is an atmospheric form of mercury (Hg)—a toxic heavy metal—that is naturally released in volcanic environments. Research with wild mice demonstrates that chronic exposure to a hydrothermal volcanic environment leads to the bioaccumulation of Hg in the lungs, but also in both the central (CNS) and peripheric (PNS) nervous systems, with marked indications of neurotoxicity. Studies addressing human exposure to volcanogenic Hg⁰ are scarce, hence its risks are still unknown. This study aims to evaluate the level of exposure to Hg⁰ in children living in a volcanically active environment. Methodology and main findings: Two groups of school-aged children (from 6 to 9 years old) were part of this study: one with children inhabiting a hydrothermal area (exposed group) and another with children inhabiting an area without volcanic activity (non-exposed group). Hair samples were collected from each individual for Hg level analysis. It was found that the levels of Hg in the hair of exposed children were 4.2 times higher than in that of non-exposed children (≈1797.84 ± 454.92 ppb vs. 430.69 ± 66.43 ppb, respectively). Conclusion: Given the vast health risks Hg poses, the need to monitor the health of populations inhabiting volcanically active areas is highlighted. Because little is known about the fate, modifications, and effects of Hg⁰ in the human body, particularly regarding its effects on the nervous system in children, the development of further research within the scope is strongly encouraged. Full article

Background/Objectives: Chronic wounds pose a significant health concern, with their prevalence increasing due to various etiologies. The global aging population further contributes to this rise, placing a substantial burden on healthcare systems in developed countries. This work aimed to develop new therapeutic options in the form of creams and dressings based on honey, gellan gum, and hyaluronic acid for preventing and treating chronic wounds across all stages. Methods: To address this, after the formulations were developed, in vitro cytocompatibility was determined. To confirm biocompatibility, an ovine wound model was used: full-thickness excisional wounds were treated with three formulations, namely gellan gum and honey sponges (GG-HNY), gellan gum, honey and hyaluronic acid sponges (GG-HA-HNY) and a honey-based cream (cream FB002). Daily assessments, including visual evaluation and wound scoring, were conducted for 30 days. Following the study period, tissues were collected for histological analyses. Results: The macroscopic examination revealed that all therapeutic groups facilitated lesion closure. Lesion size reduction, granulation tissue disappearance, and scar tissue development were consistent across all groups, with the group receiving cream demonstrating an advanced stage of healing. All groups achieved substantial wound closure by day 30, with no significant differences. Histopathological analysis following ISO standards revealed that GG-HA-HNY had the lowest ISO score, indicating minimal reactivity and inflammation, which corroborated the cytocompatibility. Conclusions: Combining these insights with previous findings enhances our understanding of wound regeneration dynamics and contributes to refining therapeutic strategies for chronic wounds. The formulations were designed to balance therapeutic efficacy with cost-effectiveness, leveraging low-cost raw materials and straightforward production methods. Full article

This study explores the prevalence and antibiotic resistance of urinary tract infections (UTIs) in pregnant women in central Portugal. A retrospective observational study was conducted on 201 positive urine cultures from pregnant women at a hospital center between January 2018 and December 2022. The data collected included age, hospital admission source, history of antibiotic therapy, catheterization status, identity of bacterial isolates, and their antibiotic profile. The most common bacterial strains were Escherichia coli (52.4%) and Streptococcus agalactiae (16.9%). In terms of antibiotic resistance, Escherichia coli demonstrated complete sensitivity to ertapenem, while Streptococcus agalactiae showed sensitivity to four antibiotics, including trimethoprim/sulfamethoxazole. Notably, most infections occurred in the third trimester, underscoring the need for continuous monitoring throughout pregnancy. This study emphasizes the importance of tailored treatment strategies to manage UTIs in pregnancy effectively, reducing the potential maternal and fetal complications. These findings contribute to regional data on UTI management in pregnant populations and aim to support improved healthcare practices. These regional data provide a solid foundation for optimizing healthcare practices in pregnant women, suggesting targeted approaches to combat antibiotic resistance and improve maternal–fetal safety during UTI treatment. Full article

The lesser mealworm, Alphitobius diaperinus, is a widespread pest in poultry farming, causing economic losses and facilitating the spread of pathogens. Current control methods rely heavily on synthetic insecticides, which raise environmental concerns and contribute to resistance. This study investigated the insecticidal potential of Moringa oleifera seed preparations, including an aqueous extract (AE), a protein-enriched fraction (PF), and an isolated lectin (WSMoL), against A. diaperinus. Contact assays demonstrated that AE and PF reduced adult survival by up to 90% and 100%, respectively, while WSMoL was effective only against larvae, causing 48% mortality. Ingestion assays showed that AE and PF reduced adult survival by 60% and 30%, respectively; impaired diet utilization; and caused significant weight loss. WSMoL exhibited antinutritional effects, including a 94% reduction in trypsin-like activity, but did not cause lethality, although it may impair long-term survival. Midgut histological analysis revealed epithelial disorganization, vacuolization, and nuclear condensation in the treated insects. These findings suggest that M. oleifera seed preparations are promising candidates for sustainable pest control, offering both lethal and sublethal effects on A. diaperinus. Future research should focus on the development of formulations and long-term impact assessments on pest populations and non-target organisms, paving the way for eco-friendly alternatives in integrated pest management. Full article