Search Results (59)

Background: The ankle joint plays a key role in stabilizing the lower limb during interaction with ground reaction forces. Instability can result in pain, weakness, and impaired movement. Although assessing ankle stability is important, few studies examine existing in vivo methodologies for dynamic load assessment, limiting effective injury management. Objective: To identify in vivo techniques using objective measurement tools for assessing ankle stability during dynamic exercise. Methods: A scoping review was performed based on PRISMA-ScR criteria. Five databases—PubMed, PEDro, Embase, SPORTDiscus, and CDSR—were searched from inception to September 2024. Results: Out of 1678 records, 32 studies met the inclusion criteria. A total of 1142 subjects were included: 293 females (25.6%), 819 males (71.7%), and 30 unspecified (2.62%). Six categories of dynamic exercise were identified: analytical, functional, balance, stair climbing, running, and walking. The techniques used included 3D motion capture, force and pressure platforms, dynamometry, electromyography, accelerometers, pressure and speed sensors, instrumented treadmills, and inertial measurement units. Conclusions: The 3D motion capture systems (240 Hz) and the force platforms (1000 Hz) were most frequently used in functional tasks and walking. Combining these with multisegmented foot models appears optimal, though tool selection depends on study goals. This review enhances our understanding of ankle stability assessment. Full article

Background/Objectives: This study aimed to translate the Foot Function Index Revised Short Form (FFI RSF-Sp) into Spanish, perform a transcultural adaptation, and validate the resulting questionnaire on foot and ankle function. The revised instrument, derived from the original version of the FFI, incorporates additional subscales on ankle stiffness and overall quality of life that help produce a more comprehensive assessment and overcome some limitations. Methods: The final sample consisted of 306 participants from Malaga and Seville (Spain), all aged over 18 years and native Spanish speakers. These participants also completed the FAAM-Sp and VASFA-Sp validated questionnaires. The measurement properties of FFI RSF-Sp were evaluated in accordance with the Consensus-Based Standards for the Selection of Health Status Measurements Instruments (COSMIN) recommendations. Results: Factor analysis confirmed the existence of a five-factor structure, explaining 67.6% of the total variance, with an RMSEA of 0.0785 and a Tucker–Lewis index of 0.874. Internal consistency was excellent, with an overall Cronbach’s alpha of 0.963 and subscale values ranging from 0.776 to 0.886. Furthermore, moderate to strong correlations were obtained with the VASFA-Sp (r = −0.651) and FAAM ADL (r = −0.737, p < 0.001), and the obtained area under the curve value was 0.806 (p < 0.001), confirming the discriminatory capacity of the instrument. Conclusions: The FFI RSF-Sp is a valid, reliable, and sensitive instrument for assessing foot and ankle function in a Spanish-speaking population. The incorporation of additional dimensions improves the understanding of dysfunction, and the robust psychometric indicators of this questionnaire make it appropriate for use in clinical and research settings, facilitating the detection of functional alterations and enhancing treatment follow up. Full article

Fish are generally rich sources of n-3 polyunsaturated fatty acids such as EPA and DHA, and although the edible part (fillet) has been analyzed in many species, less is known about the composition of fish by-products. The analysis of these materials allows them to be evaluated as raw sources of EPA and DHA, thus contributing to sustainable practices to produce healthy oils for human consumption. This work provides information on the fatty acid profiles, lipid quality indices and α-tocopherol, squalene and cholesterol contents in fillets, heads, bones and viscera of three fish species (anchovy, chub mackerel and Chilean jack mackerel). Samples were lyophilized and lipids were extracted using either the Folch or Hara and Radin methods. FA profiles were obtained by gas chromatography coupled with flame ionization detection, and tocopherol, squalene and cholesterol analyses were performed by high performance liquid chromatography with UV-visible detection. The highest levels of EPA were found in anchovy fillet (18.9–20.6%) and bone (14.7%), while DHA was more abundant in anchovy fillet (16.9–22.0%) and Chilean jack mackerel fillet (15.4–16.6%) and bone (13.1–13.8%). α-Tocopherol, squalene and cholesterol contents ranged from 0.18 to 1.35 mg/100 g, 0.07 to 0.80 mg/100 g and 30.46 to 246.17 mg/100 g, respectively, in the different tissues analyzed from the three fish species. Full article

Growing concern about food quality and safety has driven the search for natural food additives. Furthermore, maqui leaf residue (MLR), recovered from infusions that have long been part of traditional Chilean herbal medicine, still conserves a high content of phenolic compounds. The aim of this study was to optimize the ultrasound-assisted extraction (UAE) of phenolics from MLR and evaluate their potential effect on salmon preservation. The optimized parameters for UAE (6 min, MLR:Water = 1:30, and 70 W), obtained by using a Box–Behnken design, rendered the highest total phenolic content and antioxidant capacity. Furthermore, UAE showed a higher concentration of phenolic compounds compared to conventional extraction techniques, as evaluated by UPLC-ESI-MS/MS. The salmon with MLR had up to four times lower levels of thiobarbituric acid reactive substances, induced by ozonated water treatment, than the sample without this natural antioxidant. Moreover, MLR exhibited similar or superior effectiveness compared to synthetic antioxidants, such as butylated hydroxyanisole (BHA), 3,5-Di-tert-4butylhydroxytoluene (BHT). This study highlights the use of short-time aqueous ultrasound extraction as an environmentally friendly technology that allows the recovery of phenolic compounds from MLR, with potential application as a natural preservative that may replace or decrease the use of BHA and/or BHT. Full article

London rocket (Sisymbrium irio) is a wild green consumed globally, yet its phytochemical composition remains underexplored. In this study, we analyzed the leaves of wild S. irio plants and those grown in controlled environments (GCE) with varying electrical conductivities (EC) and light spectra. Plants were assessed for growth, phenolic content, vitamin C, antioxidant activity, glucosinolates, and antiproliferative effects against HT-29 human colorectal cancer cells. The optimal biomass yield occurred at the EC levels of 3.0–3.5 dS m⁻¹ under Valoya^® LED light. Wild plants showed higher antioxidant activity (DPPH and ABTS assays) than GCE samples, with values of 8.03–8.67 and 6.49–6.81 mmol TE per 100 g dry weight, respectively. The vitamin C range was 50.7–84.3 and 84.5–186.9 mg 100 g⁻¹ fresh weight for GCE and wild samples, respectively. Phenolic content was higher in wild plants than in the GCE ones, with apigetrin as the primary phenolic compound. The MTT assay showed that ethanol extracts from wild plants weakly inhibited HT-29 cell growth, with a GI₅₀ of 210–380 µg mL⁻¹ after 72 h of cells exposure to plant extracts. Principal Component Analysis suggested that EC and UV exposure increase the antioxidant activity, total phenolics, and glucosinolates in wild plants, offering insights into the bioactive profiles of S. irio leaves. Full article

Rugulopteryx okamurae is an invasive brown macroalga that has recently proliferated in the western Mediterranean Sea, causing significant environmental challenges. This alga, however, contains valuable bioactive compounds—alginate, mannitol, and phlorotannins—that can serve as biofertilizers to promote plant growth and aid in bioremediation of degraded or contaminated soils. This study focused on optimizing the extraction of these compounds from R. okamurae, transforming an ecological issue into a beneficial resource. Algae samples collected from the Spanish Mediterranean coast were processed through a randomized factorial response surface design. Extraction conditions varied by time, temperature, algae-to-solvent ratio, and ethanol-to-water ratio to determine optimal yields. The highest yields achieved were 29.4, 11.9, and 0.35 g/100 g for alginate, mannitol, and phlorotannin’s under extraction conditions of 6, 6, and 3 h; 58.8, 60.0, and 60.0 °C; and an algae:solvent ratio of 1:50, 2:45, and 1.40 g/mL, respectively. Characterization of the extracted sodium alginate using ¹H-NMR, FTIR, and high-resolution electron microscopy confirmed its high purity and typical morphological features. This study highlights a sustainable approach to mitigating the invasive spread of R. okamurae while supporting soil health and sustainable agriculture. Harnessing this invasive species’ biofertilizer potential provides a dual solution, aiding marine ecosystem conservation and developing eco-friendly agricultural practices. Full article

Triacylglycerols containing medium-chain fatty acids at the sn-1,3 positions and a long-chain fatty acid at the sn-2 position (MLM-TAG) are of nutritional interest. However, they are scarce in common food sources and are usually synthesized by chemical or enzymatic methods. In this work, the enzymatic synthesis of MLM-TAG was attempted using sn-2 monoacylglycerols (sn-2 MAG) from the ethanolysis of an arachidonic acid-rich fraction from ARASCO and fatty acid ethyl esters from the ethanolysis of coconut oil as substrates. The highest yield of sn-2 MAG (23.3 mol%) was obtained after 1 h of ethanolysis with Novozym 435 lipase at 25 °C, and the best profile of the ethanolysis products of coconut oil was obtained after 24 h of reaction catalyzed by the lipase from Thermomyces lanuginosus. Regarding the enzymatic synthesis of structured TAG, the lipase from Rhizopus oryzae gave better results than those from Thermomyces lanuginosus and Rhizomucor miehei, with the sn-2 position mainly esterified with arachidonic acid (34.8%) and the sn-1,3 positions mainly esterified with capric and lauric acids (35.1%). This work focuses on a simple process for the enzymatic production of structured TAG without prior purification of the sn-2 MAG. Full article

Walnut milk residues (WMR) were investigated for the first time through their phenolic characterization including soluble (free, esterified, and etherified) phenolics and those released from their insoluble-bound form (insoluble-bound phenolic hydrolysates, IBPHs) and their antioxidant properties. Free phenolics were recovered and alkaline or acid hydrolysis were used to recover the remaining phenolic fractions. Total phenolic compounds (TPCs) and their antioxidant activity were analyzed by Folin–Ciocalteu, FRAP, and ORAC methods, respectively. Soluble phenolics (free + esterified + etherified fractions) showed a higher TPC (275.3 mg GAE 100 g⁻¹ dw) and antioxidant activity (FRAP: 138.13 µmol TE g⁻¹ dw; ORAC: 45.41 µmol TE g⁻¹ dw) with respect to the IBPH. There was a significant correlation between TPC and FRAP and ORAC values regardless of the fraction and tested sample. Phenolic acids and flavonoids were identified and quantified by ultra-performance liquid chromatography–electrospray tandem mass spectrometry (UPLC-ESI-MS/MS). Gallic acid, mainly in the free form (3061.0 µg 100 g⁻¹), was the most representative, followed by biochanin A, identified for the first time in a walnut product and mostly present in the fraction released from the esterified form (593.75 µg 100 g⁻¹). No detrimental cytotoxic impact on Caco-2 cells was observed. Hence, WMR could be considered a potential source for the development of nutraceutical and/or antioxidant food additives. Full article

This study developed, validated, and tested a simple method for tocopherol analysis on five different food matrices (sunflower oil, mackerel fillets, almonds, spinach, and avocado pulp). Tocopherol extraction from foods was carried out by the Folch method and with n-hexane, and the identification and quantification of tocopherol isoforms (α, β, γ, and δ) was performed using normal-phase liquid chromatography with ultraviolet–visible detection (NP-HPLC–UV–Vis). The normal-phase column fully separated the four tocopherol isoforms in less than ten minutes. Linearity was shown to be excellent for the four isoforms in the assayed range (10–375 ppm, R² > 0.99). Furthermore, the limits of detection (LOD) and quantification (LOQ) ranged from 0.32 to 0.63 ppm, and from 1.08 to 2.11 ppm, respectively. The intra-day and inter-day precision were assessed at different concentrations (10, 100, and 250 ppm) for each tocopherol isoform and they were within the range of acceptable values. Recovery rates were above 80% in most cases for all of the assayed food matrices, regardless of the extraction method (Folch solvents or n-hexane). α-Tocopherol was the main isoform found in all tested foods, and sunflower oil was the sample with the highest content, followed by almond, avocado pulp, mackerel fillet, and spinach. This method provides a convenient alternative for obtaining a complete profile of the four tocopherol isoforms in a variety of food matrices and for tracking the potential degradation kinetics of fortified foods during their processing and storage. Full article

Amaranthus viridis L. is a wild edible plant that occasionally is cultivated as an alternative crop because of its interest as a functional food and its adaptation to high-saline soils. In this work, leaves from A. viridis were compared with their grown in controlled environment (GCE) counterparts in a soilless system at electrical conductivities (EC) and different light exposures for assessing growth parameters, moisture, total phenolic and total flavonoid content, phenolic compound profiles, vitamin C, antioxidant activity, and antiproliferative activity against the HT–29 human colorectal cancer cell line. The highest biomass production was obtained using EC of 2.5 dS m⁻¹ and the AP67 Milk LED lamp. Vitamin C in wild samples ranged from 83.1 to 104.9 mg 100 g⁻¹ fresh weight (fw), and in GCE ones, it ranged from 112.3 to 236.7 mg 100 g⁻¹ fw. Measured by the DPPH and ABTS assays, the antioxidant activity was higher in wild than in GCE plants: the ranges for wild samples were in the 1.8–4.9 and 2.0–3.9 mmol of Trolox Equivalent (TE) 100 g⁻¹ dry weight (dw) ranges, and for GCE ones in the 1.3–1.9 and 1.5–2.2 mmol TE 100 g⁻¹ dw ranges, respectively. As for phenolic compounds, in wild samples, the range was from 14.65 to 22.70 mg 100 g⁻¹ fw, and these amounts were much higher than those found in their GCE counterparts, in which the range was from 2.58 to 5.95 mg 100 g⁻¹ fw. In wild plants three compounds, namely trans-p-coumaric acid, isorhamnetin-3-O-glucoside, and nicotiflorin, accounted for more than half of the total quantified phenolic compounds. The MTT assay revealed concentration- and time-dependent inhibitory effects on HT–29 cells for all checked extracts. Cancer cells were less influenced by extracts from GCE plants, which showed higher GI₅₀ compared to wild plants. This work improves knowledge on the growth parameters, phytochemical profiles, and biological activities of wild and GCE A. viridis. Full article

The shoots of Asparagus L. are consumed worldwide, although most species belonging to this genus have a restricted range, and several taxa remain unstudied. In this work, a total of four taxa from different locations were scrutinized and compared with cultivated A. officinalis. All shoots were screened for saponins via LC-MS, and in vitro antiproliferative activities against the HT-29 colorectal cancer cell line were assessed via the MTT assay. The total saponins (TS) contained in the crude extracts ranged from 710.0 (A. officinalis) to 1258.6 mg/100 g dw (A. acutifolius). The richness of the compounds detected in this work stands out; a total of 47 saponins have been detected and quantified in the edible parts (shoots) of five taxa of Asparagus. The structure of all the saponins found present skeletons of the furostane and spirostane type. In turn, the structures with a furostane skeleton are divided into unsaturated and dioxygenated types, both in the 20–22 position. The sum of dioscin and derivatives varied largely among the studied taxa, reaching the following percentages of TS: 27.11 (A. officinalis), 18.96 (A. aphyllus), 5.37 (A. acutifolius), and 0.59 (A. albus); while in A. horridus, this compound remains undetected. Aspachiosde A, D, and M varied largely among samples, while a total of seven aspaspirostanosides were characterized in the analyzed species. The hierarchical cluster analysis of the saponin profiles clearly separated the various taxa and demonstrated that the taxonomic position is more important than the place from which the samples were acquired. Thus, saponin profiles have chemotaxonomic significance in Asparagus taxa. The MTT assay showed dose- and time-dependent inhibitory effects of all saponins extracts on HT-29 cancer cells, and the strongest cell growth inhibition was exercised by A. albus and A. acutifolius (GI₅₀ of 125 and 175 µg/mL). This work constitutes a whole approach to evaluating the saponins from the shoots of different Asparagus taxa and provides arguments for using them as functional foods. Full article

We describe a family of paraxial and quasi-monochromatic optical wave packets with finite energy and smoothly shaped amplitude in space and time that develops a singularity in the intensity when spatio-temporally focused by imparting a converging spherical wavefront and a negative temporal chirp. This singular behavior upon ideal focusing is manifested in actual focusing with finite apertures and in media with high-order dispersion with “exploding” behavior featuring an indefinitely increasing concentration of the energy when opening the aperture radius, thus exercising continuous control on the focal intensity and spatial and temporal resolution. These wave packets offer a new way of focusing that outperforms what can be achieved with standard Gaussian wave packets in terms of focal intensity and resolution, providing new possibilities in applications where energy concentration and its control are crucial. Full article

The seeds of Boraginaceae species constitute a source of γ-linolenic acid (GLA, 18:3n-6)-rich oils, mainly those of Borago officinalis. However, there are many wild unexplored Boraginaceae taxa that are potentially GLA producers. This work scrutinizes the seeds of several Greek Boraginaceae species for fatty acids (FAs), phenolic compounds, antioxidant activity, and in vitro antiproliferative activity against HT-29 colorectal cancer cells. The FA profiles were checked by GC-FID, and GLA (FA% of total FA) highlights in Symphytum bulbosum (26.2), S. creticum (23.2), and Lithodora zahnii (17.4). The total FA content ranged from 9.2 in Alkanna methanaea to 27.3 g/100 g of seeds in Alkanna corcyrensis. The antioxidant activity of the water/methanol extracts, evaluated by the ABTS and DPPH methods, was in line with other GLA producer seeds. Phenolic acids were analyzed by HPLC-DAD and LC-MS, and rosmarinic acid ranged from 160.2 in S. bulbosum to 479.7 mg/100 g in L. zahnii. The MTT assay showed dose- and time-dependent inhibitory effects of the water/methanol extracts against HT-29 cancer cells, especially those of C. major and L. zahnii (GI₅₀ of 600 and 375 μg/mL for 72 h-exposed cells). This work constitutes the first approach to evaluate the seeds of Boraginaceae taxa from Greece as functional oil providers. Full article

Spent coffee grounds (SCG) are a type of food waste and are produced in abundance around the world. However, their utilization as a soil organic amendment is challenging due to their phytotoxic effect. In the present work, the impact of agronomic biofortification on Dutch cucumbers was investigated using different chemically modified SCG and analyzing their effects on iron contents, their capacity for releasing antioxidants, and the production of short-chain fatty acids after in vitro digestion–fermentation. The results indicated variations in the iron contents and chemical compositions of cucumbers according to the treatment groups. Functionalized and activated hydrochar from SCG increased Fe levels in cucumbers. Although activated hydrochar obtained at 160 °C and functionalized with Fe showed the highest iron supply per serving, differences in antioxidant capacity and short-chain fatty acid production were observed between the groups. It is concluded that growing conditions and the presence of iron may significantly influence the contribution of these cucumbers to the dietary intake of nutrients and antioxidants, which could have important implications for human health and nutrition. Full article

Spent coffee grounds (SCGs) are a food waste with a large generation around the world. However, their utilization as a soil organic amendment is difficult due to their phytotoxic effect. In the present work, the impact of agronomic biofortification on Dutch cucumbers was studied by using different chemically modified SCGs, analyzing their effects on Zn content, the release of antioxidant capacity and the production of short-chain fatty acids after in vitro digestion–fermentation. The results indicated variations in the Zn content and chemical composition of cucumbers according to the treatment groups. The functionalized with Zn and activated SCGs were able to increase Zn levels in cucumbers. Meanwhile, the activated hydrochar obtained at 160 °C and the activated and functionalized with Zn SCGs showed the highest Zn supply per serving. Differences in the antioxidant capacity and short-chain fatty acid production were observed between the groups. It is concluded that the growing conditions and the presence of Zn may significantly influence the contribution of these cucumbers to the dietary intake of nutrients and antioxidants, which could have important implications for human health and nutrition. Full article