Search Results (65)

The Songhua River Basin (SRB) in Northeast China is a high-latitude basin experiencing significant snow cover changes under global warming. This study quantified spatiotemporal changes in snowmelt in the SRB (1961–2020). A specific focus was placed on the changes at event scale, including frequency, magnitude and duration, that have been underexplored in previous work. Correlations between snowmelt and key driving factors were assessed to identify the dominant controls governing the melt process. A significant elevation-dependent decreasing trend in annual snowmelt was found over the decades, with the decrease most pronounced at lower elevations. Relative to the baseline period (1961–1990), the snowmelt dates during 1991–2020 advanced, with the 25%, 50%, and 75% cumulative levels occurring 9, 6, and 2 days earlier, respectively. Seasonally, snowmelt increased significantly in early spring (February to March) but decreased notably in late spring (April to May). Snowmelt events exhibited reduced frequency, total volume, peak value, and mean rate, along with fewer extreme events. The strongest correlation across snowmelt event types was found with mean snow depth for complete depletion and with accumulated sunshine duration for incomplete depletion, while Rain-on-Snow Melt events were most closely associated with sunshine and temperature. This study can provide a crucial reference for sustainable water management and spring agricultural irrigation in the SRB. Full article

Biped robots based on parallel mechanisms hold great potential for applications in complex terrains. Based on a 4-UPU parallel mechanism, this paper proposes a novel biped robot that achieves alternating bipedal locomotion and turning with only six actuators by employing fixed/moving platform switching and following an “upper platform + lower foot” continuous gait strategy. Using the influence coefficient method, the first order and second order kinematic influence coefficient matrices of the biped robot were derived. Based on the principle of virtual work, a dynamic model of the robot was formulated, and its validity was verified through numerical simulations. The dynamic performance of the robot was further evaluated using the Dynamic Manipulability Ellipsoid (DME) index, while its stability during step-climbing and turning was analyzed using the Zero-Moment Point (ZMP) method. The results demonstrate that the dual-platform biped robot features a rational structure and exhibits robust stability during step-climbing and turning. Full article

The pervasive environmental contamination by micro- and nanoplastics (MNPs) presents a formidable analytical challenge, necessitating the development of rapid and sensitive detection methods. While conventional techniques often suffer from limitations in sensitivity and throughput, fluorescent probe-based technology has emerged as a powerful alternative. This review charts the evolution of these probes, from initial stains relying on hydrophobic adsorption to advanced molecular designs engineered for specific chemical recognition. We critically examine key operational mechanisms, including the solvatochromic response of Nile Red, polarity-discriminatory probes enabling a “microplastic rainbow,” and targeted systems achieving turn-on fluorescence via restriction of intramolecular rotation. Furthermore, we highlight cutting-edge signal enhancement strategies, such as plasmon- and metal-enhanced fluorescence, which amplify detection to the femtogram level. Special emphasis is placed on the distinct challenges posed by nanoplastics, including their propensity for aggregation in aqueous matrices that exacerbates false positives and their superior ability to breach biological barriers, and how AIE luminogens and PEF/MEF strategies mitigate these issues through enhanced signal-to-noise ratios and subcellular resolution, differing from their application to microplastics. Critically, we address the imperative for low-toxicity probe designs, emphasizing biocompatibility and biodegradability criteria to facilitate safe, long-term in vivo tracking and widespread ecological surveillance. The integration of these sophisticated probes with smart, “activate-on-target” systems is paving the way for next-generation MNP analysis, offering critical insights for environmental monitoring and toxicological assessment. Full article

Dendrobium is an orchid genus with high economic and ecological importance, but its taxonomy based on morphology remains controversial. Dendrobium linawianum, a critically endangered species with both ornamental and medicinal value, represents a key taxon within this genus. However, its phylogenetic relationship has long been unplaced due to similar morphological traits. Despite its conservation and taxonomic importance, its complete chloroplast genome has not been previously characterized. Here, we newly sequenced and assembled the complete chloroplast genome of D. linawianum. The 150,497 bp genome exhibits a typical quadripartite structure, encoding 119 genes. A total of 161 simple sequence repeats (SSRs) were identified, predominantly mononucleotide and dinucleotide motifs. Condon usage analysis revealed leucine as the most abundant amino acid. Phylogenetic analysis based on complete chloroplast genome sequences strongly supported the close relationship of D. linawianum with D. hercoglossum, D. thyrsiflorum, and D. moniliforme, resolving its taxonomic position within the genus. The complete chloroplast genomes successfully resolved the phylogenetic relationships among 35 Dendrobium species, demonstrating their efficacy as powerful molecular markers for resolving taxonomic ambiguities within this morphologically complex genus. Our findings provide a genomic foundation for precise species identification and molecular breeding of D. linawianum, and enhance understanding of phylogenetic relationships in this taxonomically challenging group. Full article

RNA interference (RNAi) represents a promising pest control strategy, applicable to both insect-resistant genetically modified (IRGM) crops and sprayable RNAi insecticides. These products can achieve sequence-specific gene silencing and require rigorous environmental risk assessment (ERA) prior to approval. However, current environmental safety assessments of RNAi products and other RNAi experiments frequently use double-stranded EGFP (dsEGFP) as a negative control, while suitable RNAi-based positive controls are lacking. Sometimes conventional chemical toxins (e.g., chlorpyrifos) or protein inhibitors (e.g., trypsin inhibitors) are used as substitutes, but their distinct mechanisms, persistence, and metabolism make them inappropriate for RNAi-specific evaluations. In this study, we evaluated the suitability of RNAi-based positive controls for assessing non-target effects on Harmonia axyridis, a widely distributed predatory beetle used as a bioindicator in biosafety assessments. Under laboratory conditions, we tested one microRNA (miR-92a) and two double-stranded RNAs (dsHaSnf7 and dsHaDiap1) for their effects on H. axyridis. Injection of miR-92a showed no significant difference in mortality compared to controls, whereas dsHaSnf7 and dsHaDiap1 significantly reduced survival rates and target gene expression, as confirmed by qPCR. These findings suggest that HaSnf7 and HaDiap1 are suitable candidate genes for establishing RNAi-specific positive controls in environmental risk assessments of RNAi-based products. Full article

Acmella radicans (Jacquin) R.K.Jansen is an annual herb native to Central America. In China, it is becoming increasingly invasive and often co-occurs with the native congener A. paniculata (Wall. ex DC.) R.K.Jansen in some habitats. In order to understand the invasion mechanism of A. radicans, we investigated the growth parameters of both the invasive A. radicans and the native congener, A. paniculata, under different light conditions (5%, 25%, 50%, 75%, and 100% of light availability) using potted plants in a glasshouse. Light level, plant species, and their interaction were significant, with plant species generally having a greater effect than light level. Acmella radicans and A. paniculata showed great phenotypic plasticity to various light intensities and had a similar trend with increased shade. The plasticity indices of all parameters of A. radicans, except for branch length and inflorescence number, were greater than those of A. paniculata under the same light intensity. The physiological parameters for A. radicans under both favorable (high light intensity) and unfavorable (low light intensity) conditions showed less inhibition than those of A. paniculata. All these responses indicated that A. radicans had greater phenotypic plasticity and higher adaptability to low light, which may contribute to its invasion success. Full article

High-speed laser cladding technology is an innovative process that reduces costs and enhances coating quality. In this study, CoCrMo wear-resistant coatings were fabricated on a 40Cr steel substrate using high-speed laser cladding technology and compared to CoCrMo coatings produced by traditional methods. The effects of both processes on the microstructure, nanoindentation characteristics, and wear behavior of CoCrMo coatings were examined. The results show that the phase compositions of both coatings include γ-Co solid solution and ε-Co solid solution. The high cooling rate of high-speed laser cladding significantly suppressed Mo precipitation, enhancing Mo solid solution strengthening. Additionally, the fine-grain strengthening effect induced by the high cooling rate contributed significantly to the coatings’ mechanical properties. The nano-hardness of the HS-CoCrMo coatings reached approximately 5.18 ± 0.23 GPa, 1.2 times higher than that of the N-CoCrMo coatings. Furthermore, the generalized hardness, H/E ratio, and H³/E² ratio of HS-CoCrMo coatings were improved. This increase in nano-hardness significantly boosted the wear resistance of HS-CoCrMo coatings, yielding an average friction coefficient of approximately 0.466, with wear volume and specific wear rate values of 6.55 × 10⁶ μm³ and 0.87 × 10⁻⁵ mm³/N·m, respectively, outperforming the N-CoCrMo coatings. The main wear mechanisms for the HS-CoCrMo coatings were abrasive wear, adhesive wear, and oxidative wear. In conclusion, high-speed laser cladding technology produces high-performance, wear-resistant coatings with high productivity, offering broader application prospects for the metallurgical and power industries, while effectively reducing production cycles and usage costs. Full article

To improve the mobility of mobile robots in complex terrain environments, a novel 2-UPS&PRPU parallel mechanism is proposed, for which the parallel mechanism branched-chain decomposition and synthesis method is adopted. Based on the structural characteristics of the Hooke joint kinematic substructure, an inverse solution calculation for the mechanism is carried out, and the parameters of the simulation model are formulated to determine the workspace of the parallel mechanism. The linear velocity dexterity and minimum output carrying capacity of the parallel mechanism are analyzed, allowing the optimal parameters of the mechanism to be selected through dimension optimization, thus greatly improving the mechanism’s linear velocity dexterity and carrying capacity. The results show that the proposed parallel mechanism can satisfy the mobility requirements of mobile robots. Full article

Background: The open-stance forehand is a fundamental technique in tennis, playing a crucial role in competitive performance. Its execution depends heavily on lower limb coordination and neuromuscular control. Athletes of different skill levels often display distinct muscle activation strategies. This study employs non-negative matrix factorization (NMF) to analyze lower limb muscle synergy patterns during the forehand open stance across skill levels and explores their potential influence on stroke performance. Methods: A total of 30 tennis players, including 15 elite and 15 amateur athletes, participated in this study. Surface electromyography (sEMG) was used to record the activity of major lower limb muscles during the forehand open stance. Muscle synergy patterns were extracted using NMF, and K-means clustering was applied to classify synergy patterns. Independent sample t-tests were conducted to examine differences between muscle synergies. Results: Significant differences (p < 0.05) were observed in the spatial characteristics of each synergy component across different movement phases. However, temporal characteristics showed a significant difference only in Syn2 during the mid-phase of the backswing (BS) (56.2–60.4%) (p = 0.033). Conclusions: Elite athletes exhibited more optimized and stable muscle activation patterns, enabling more efficient coordination of major muscle groups. Based on sEMG decomposition and muscle synergy analysis, these activation patterns may contribute to improved stroke efficiency and energy transfer and potentially reduce the risk of sports-related injuries. Full article

The kinematic and kinetic performance of tennis players differs across skill levels, with joint range of motion (ROM), moments, and stiffness being strongly linked to injury risk. Focusing on the biomechanical characteristics of lower-limb joints throughout the landing stage, especially among athletes of different skill levels, aids in understanding the link between injury risk and performance level. This study recruited 15 male campus tennis enthusiasts and 15 male professional tennis players. The kinematic and kinetic differences between amateur and professional players during the landing phase of the tennis serve were analyzed using SPM1D 0.4.11 and SPSS 27.0.1, with independent-sample t-tests applied in both cases. Throughout the tennis serve’s landing stage, the professional group exhibited significantly greater sagittal plane hip-joint stiffness (p < 0.001), horizontal plane moment (59~91%; p = 0.036), and a significantly higher peak moment (p = 0.029) in comparison with the amateur group. For the knee joint, the professional group exhibited significantly larger ROM in flexion–extension (0~82%; p = 0.003); along with greater ROM (0~29%; p = 0.042), moment (12~100%; p < 0.001), peak moment (p < 0.001) in adduction-abduction; and internal–external rotational moments (19~100%; p < 0.001) were markedly higher. The professional group showed significantly higher ankle joint ROM (p < 0.001) and moments (6~74%; p = 0.004) in the sagittal plane, as well as greater horizontal-plane ROM (27~67%; p = 0.041) and peak moments (p < 0.001). Compared with amateur tennis players, professional tennis players exhibit greater ROM, joint moments, and stiffness in specific planes, potentially increasing their risk of injury during the landing phase. Full article

In China, the current policy of the financial sector on water conservation and management is being vigorously pursued; therefore, efficient synergy between the two systems is of great significance. In this study, the coupling and coordination degree (CCD) between reclaimed water usage efficiency (RWUE) and high-quality development of the financial sector (HQDFS) was assessed using a coupling coordination model with panel data from 27 provinces in China during 2010–2021, and a more in-depth coupling and coordination relationship (CCR) was carried out using a spatiotemporal evolution methodology and PVAR model. The results of this study show the following: (1) CCD exhibits a continuous upward trajectory. At the end of the study period, the eastern, central, western, and northeastern regions moved to the primary coordination level. (2) The eastern and northeastern regions show an increasing trend in absolute differences and polarization. Meanwhile, the central region experiences a gradual rise in polarization. (3) The elliptical plot of the CCD’s standard deviation tends toward a circular shape with a positive aspect ratio. An expanding trend of absolute differences and polarization is observed in the eastern and northeastern regions. (4) The PVAR results show that the two systems can promote each other in the early stages and have a negative impact in the later stages. This study provides policy recommendations for a balanced development of the two systems and the formulation of regional development strategies based on the state of coupling and coordination between the two. Full article

Based on the reconfigurable revolute (rR) pair, a reconfigurable decoupled parallel mechanism is proposed, which is composed of three serial chains. Traditional serial chain 1 and 2 are of types PRRR and URC, respectively, where P denotes a prismatic pair, R denotes a revolute pair, U denotes a universal pair, and C denotes a cylindric pair. The reconfigurable serial chain 3 can switch between PRRP and RRPP configurations by changing the axis of reconfigurable pair rR, thus enabling the parallel mechanism to switch between two motion modes of 2T1R (where R represents rotation and T represents translation) and 1T2R. By investigating the relationship between the mechanism’s motion output, input, and the Jacobian matrix, it is verified that the parallel mechanism is a completely decoupled mechanism in the 2T1R motion mode and a partially decoupled mechanism in the 1R2T motion mode. Finally, the performance indexes of the mechanism in both motion modes were discussed using screw theory, and the dimensions of the mechanism were optimized in scale, thereby enhancing the motion performance of the parallel mechanism. The results indicate that the decoupling characteristics of the reconfigurable parallel mechanism have significant advantages in both 2T1R and 1T2R motion modes, providing a theoretical basis for the study of reconfigurable and decoupled parallel mechanisms. Full article

Background: Barbell squats are commonly used in strength training, but the anterior–posterior displacement of the Center of Mass (COM) may impair joint stability and increase injury risk. This study investigates the key factors influencing COM displacement during different squat modes.; Methods: This study recruited 15 male strength training enthusiasts, who performed 60% of their one-repetition maximum (1RM) in the Front Barbell Squat (FBS), High Bar Back Squat (HBBS), and Low Bar Back Squat (LBBS). Joint moments at both the hip, knee, and ankle were collected using a motion capture system and force plates, and a factor regression analysis was conducted using SPSS.; Results: In the FBS, primary factors influencing COM displacement included right knee adduction–abduction (38.59%), knee flexion–extension (31.08%), and hip internal–external rotation (29.83%). In the HBBS, they were right ankle internal–external rotation (19.13%), hip flexion–extension (−19.07%), and left knee flexion–extension (19.05%). In the LBBS, the key factors were left knee adduction–abduction (27.82%), right ankle internal–external rotation (27.59%), and left ankle internal–external rotation (26.12%).; Conclusion: The study identifies key factors affecting COM displacement across squat modes, with knee flexion–extension being dominant in the FBS and hip moments more significant in the HBBS and LBBS. These findings have implications for optimizing squat training and injury prevention strategies. Full article

The formation of animal breeds usually begins with a small subsample from their ancestral population. Deleterious mutations accumulate in the population under genetic drift, inbreeding, and artificial selection during the development and maintenance of traits desired by humans. White raccoon dogs are among the most popular breeds of farmed raccoon dogs, but white raccoon dogs are more susceptible to disease and have a lower reproductive ability. However, the accumulation of deleterious mutations in this white breed is largely unknown. By analyzing and comparing whole-genome sequencing data from 20 white raccoon dogs and 38 normal raccoon dogs, we detected an increased occurrence of loss-of-function (LoF) mutations in white raccoon dogs compared with normal raccoon dogs. With the finding of a significantly higher dosage of homozygous missense mutations in the white raccoon dog genome, we detected a greater fitness cost in white raccoon dogs. Although a much higher FROH level for ROH fragments longer than 1 Mb has been reported in white raccoon dogs, we did not detect a genetic signal of genetic purging in white raccoon dogs. This study provides valuable genomic resources and new insights into the accumulation of mutation loads in farmed raccoon dogs. Full article

A low-cost, wavelength-tunable single-longitudinal-mode (SLM) thulium–holmium co-doped fiber laser (THDFL) in a 2 μm band with a simple structure is described in the present paper. To obtain a stable SLM and narrow laser linewidth, a five-coupler-based three-ring (FCTR) filter is utilized in the ring cavity of the fiber laser. Tunable SLM wavelength output from THDFLs with kHz linewidths can be achieved by designing the FCTR filter with an effective free-spectral range and a 3 dB bandwidth at the main resonant peak. The measurement results show that the laser is in the SLM lasing state, with a highly stabilized optical spectrum, a linewidth of approximately 9.45 kHz, an optical signal-to-noise ratio as high as 73.6 dB, and a relative intensity noise of less than −142.66 dB/Hz. Furthermore, the wavelength can be tuned in the range of 2.6 nm. The proposed fiber laser has a wide range of applications, including coherence optical communication, optical fiber sensing, and dense wavelength-division-multiplexing. Full article