Search Results (17)

Soil degradation and erosion pose significant threats to agricultural sustainability in fragile ecosystems, such as the Loess Plateau in northern China. This study examines the long-term impacts of fertilization regimes and land-use systems on soil health, focusing on soil aggregate stability, fertility, and crop productivity. Six treatment combinations were evaluated in our study, including three continuous alfalfa fields (AL-CK, AL-P, and AL-NPM) and three continuous wheat fields (WH-NPM, WH-NP, and WH-P), each representing a combination of land use and three fertilization treatments: (1) no fertilization (CK), (2) inorganic fertilization (120 kg ha⁻¹ N, 60 kg ha⁻¹ P-NP), and (3) a combination of organic and inorganic fertilization (75 t ha⁻¹ cow manure-NPM). Soil samples were collected from three depths (0–10 cm, 10–20 cm, and 20–30 cm) to assess physical and chemical properties. We evaluated the long-term effects of different fertilization treatments on soil stability, fertility, and crop yield to explore the interactions among soil’s physical and chemical properties under two land-use types and to assess the effectiveness of combined organic and inorganic fertilization strategies in improving soil health and mitigating erosion in vulnerable landscapes. The study revealed significant depth-specific variations with surface layers (0–10 cm) showing the greatest improvement under NPM treatments, particularly in continuous alfalfa fields, which exhibited higher soil fertility, improved soil structure, and crop yield. In contrast, continuous wheat fields with minimal fertilization demonstrated significantly lower soil quality and productivity. Using the combination of mineral fertilizers and organic amendments, such as cow manure, proved to be the most effective strategy for significantly enhancing nutrient availability and overall soil health. Partial Least Squares Modeling (PLS-M) and Mantel analysis highlighted the critical role of fertilization management in maintaining soil quality, boosting crop productivity, and mitigating erosion in high-risk areas. This study emphasizes the importance of integrated nutrient management for sustainable land use and soil conservation in erosion-prone regions. Full article

Background/Objectives: Inflammation and analgesia are two prominent symptoms and often lead to chronic medical conditions. To control inflammation and analgesia, many marketed drugs are in practice but the majority of them have severe side effects. Methods: This study involved the synthesis of a pivalate-based Michael product and evaluated it for in vitro COX-1, COX-2, and 5-LOX inhibitory potentials using specific assays. Molecular docking studies were also assessed. Based on the in vitro results, the compound was also subjected to in vivo anti-inflammatory and antinociceptive studies. Results: The pivalate-based Michael product (MAK01) was synthesized by an organocatalytic asymmetric Michael addition of ethyl isobutyrate to N-phenylmaleimide with an isolated yield of 96%. The structure of the compound was confirmed through ¹H and ¹³C NMR analyses. The observed IC₅₀ values for COX-1, COX-2, and 5-LOX were 314, 130, and 105 μg/mL, respectively. The molecular docking studies on the synthesized compound showed binding interactions with the minimized pockets of the respective enzymes. In a carrageenan model, a percent reduction in edema when administered at 10 mg/kg (a reduction of 33.3 ± 0.77% at the second hour), 20 mg/kg (a reduction of 34.7 ± 0.74% at the second hour), and 30 mg/kg (a reduction of 40.58% ± 0.84% after the fifth hour) was observed. The compound showed a significant response at concentrations of 50, 100, and 150 mg/kg with latency times of 10.32 ± 0.82, 12.16 ± 0.51, and 12.93 ± 0.45 s, respectively. Conclusion: In this study, we synthesized a pivalate-based Michael product for the first time. Moreover, based on its rationality and potency, it was found to be an effective future medicine for the management of analgesia and inflammation. Full article

This study investigated the stability of bipartite nonlinear fractional-order multi-agent systems (FOMASs) in the presence of false data injection attacks (FDIAs) in a hostile environment. To tackle this problem we used signed graph theory, the Razumikhin methodology, and the Lyapunov function method. The main focus of our proposed work is to provide a method of stability for FOMASs against FDIAs. The technique of Razumikhin improves the Lyapunov-based stability analysis by supporting the handling of the intricacies of fractional-order dynamics. Moreover, utilizing signed graph theory, we analyzed both hostile and cooperative interactions between agents within the MASs. We determined the system stability requirements to ensure robustness against erroneous data injections through comprehensive theoretical investigation. We present numerical examples to illustrate the robustness and efficiency of our proposed technique. Full article

Soil water infiltration is a key mechanism for meeting plant water demand and groundwater recharge cycles; however, unreasonable land use practices cause reduced infiltration capacity and greater soil erosion. To date, differences in the properties of aeolian sandy soil and Pisha sandstone soil under different utilization methods as well as in soil properties, aggregates, and infiltration among kind of soil types, remain poorly understood. In this work, 54 soil samples of cropland and grassland were selected to identify the unique characteristics of soil infiltration processes under transition from cropland to grassland and contributions of soil properties to soil infiltrability in the Loess Plateau of China. The results showed that converting cropland to grassland could enhance the stable infiltration capacity of shallow soils of aeolian sandy soil and loess soil by 43.6% and 35.7%, respectively. Compared with cropland, the root properties and soil aggregate formation of the three soil types increased during grassland use, with the largest increase in soil organic matter content (32.14%) and total porosities (6.4%). As determined by the ring knife method, the saturated infiltration capacity of Pisha sandstone soil was significantly lower than in aeolian sandy soil and loess soil (p < 0.5). Moreover, its saturated infiltration capacity of cropland was better than grassland. Spearman’s correlation analysis and structural equation modeling (SEM) revealed that soil infiltration capacity appeared to be the most influenced by soil organic matter, and aggregate structure. These results highlight that fifteen years of returning cropland to grassland is not enough to affect the infiltration ability of deep soil (≥20 cm), and this improvement requires longer term maintenance. Full article

Coordination complexes (1–4) of 2-amino-4-methylbenzothiazole and 2-amino-3-methylpyridine with Cu(CH₃COO)₂ and AgNO₃ were prepared and characterized by UV/Vis and FT-IR spectroscopy. The molecular structure for single crystals of silver complexes (2 and 4) were determined by X-ray diffraction. The coordination complex (2) is monoclinic with space group P21/c, wherein two ligands are coordinated to a metal ion, affording distorted trigonal geometry around the central Ag metal ion. The efficient nucleophilic center, i.e., the endocyclic nitrogen of the organic ligand, binds to the silver metal. Ligands are coordinated to adopt cis arrangement, predominantly due to steric reasons. The O(2) and O(3) atoms of the $N{O}_3^{-}$ group further play an important role in such type of ligand arrangement by hydrogen bonding with the NH₂ group of ligands. Complex (4) is orthorhombic, P212121, comprising two molecules of 2-amino-3-methylpyridine as ligand coordinated with the metal ion, affording a polymeric structure. The coordination behavior of the ligand is identical to that in complex 2, wherein ring nitrogen is coordinated to the metal center and bridged to another metal ion through an NH₂ group. The resulting product is polymeric in nature with the Ag metal in the backbone and ligand as the bridge. Compounds (2–4) were found to be luminescent, while 1 did not show such activity. All compounds were screened for their preliminary biological activities such as antibacterial, antioxidant and enzyme inhibition. Compounds exhibited moderate activity in these tests. Full article

This study aimed to evaluate 2-(N-((2′-(2H-tetrazole-5-yl)-[1,1′-biphenyl]-4yl)-methyl)-pentanamido)-3-methyl butanoic acid-based ester derivatives as a new class of angiotensin-II receptor antagonists. For this purpose, a series of compounds were synthesized using a variety of phenols. Their chemical characterization was established by FTIR, ¹HNMR, and ¹³CNMR techniques. The biological activities including antioxidant potentials using the DPPH assay, the antihypertensive assay, the urease enzyme inhibition assay, and the antibacterial assay using agar well diffusion methods were performed. All the new compounds showed significant free radical scavenging potentials more than the parent drug while retaining antihypertensive potentials along with urease inhibition properties. However, the AV2 test compound was found to be the most potent against hypertension. Most of the synthesized analogs showed urease inhibitory actions. Molecular docking studies were performed for all the active analogs to decode the binding detail of the ligands with receptors of the enzyme’s active site. Full article

Postaxial Polydactyly (PAP) is a congenital disorder of limb abnormalities characterized by posterior extra digits. Mutations in the N-terminal region of the Zinc finger protein 141 (ZNF141) gene were recently linked with PAP type A. Zinc finger proteins exhibit similarity at their N-terminal regions due to C2-H2 type Zinc finger domains, but their functional preferences vary significantly by the binding patterns of DNA. Methods: This study delineates the pathogenic association, miss-fold aggregation, and conformational paradigm of a missense variant (c.1420C > T; p.T474I) in ZNF141 gene segregating PAP through a molecular dynamics simulations approach. Results: In ZNF141 protein, helices play a crucial role by attaching three specific target DNA base pairs. In ZNF141^T474I protein, H1, H3, and H6 helices attain more flexibility by acquiring loop conformation. The outward disposition of the proximal portion of H9-helix in mutant protein occurs due to the loss of prior beta-hairpins at the C terminal region of the C2-H2 domain. The loss of hydrogen bonds and exposure of hydrophobic residues to solvent and helices turning to loops cause dysfunction of ZNF141 protein. These significant changes in the stability and conformation of the mutant protein were validated using essential dynamics and cross-correlation maps, which revealed that upon point mutation, the overall motion of the proteins and the correlation between them were completely different, resulting in Postaxial polydactyly type A. Conclusions: This study provides molecular insights into the structural association of ZNF141 protein with PAP type A. Identification of active site residues and legends offers new therapeutic targets for ZNF141 protein. Further, it reiterates the functional importance of the last residue of a protein. Full article

The global hydrological cycle is susceptible to climate change (CC), particularly in underdeveloped countries like Pakistan that lack appropriate management of precious freshwater resources. The study aims to evaluate CC impact on stream flow in the Soan River Basin (SRB). The study explores two general circulation models (GCMs), which involve Access 1.0 and CNRM-CM5 using three metrological stations (Rawalpindi, Islamabad, and Murree) data under two emission scenarios of representative concentration pathways (RCPs), such as RCP-4.5 and RCP-8.5. The CNRM-CM5 was selected as an appropriate model due to the higher coefficient of determination (R²) value for future the prediction of early century (2021–2045), mid-century (2046–2070), and late century (2071–2095) with baseline period of 1991–2017. After that, the soil and water assessment tool (SWAT) was utilized to simulate the stream flow of watersheds at the SRB for selected time periods. For both calibration and validation periods, the SWAT model’s performance was estimated based on the coefficient of determination (R²), percent bias (PBIAS), and Nash Sutcliffe Efficiency (NSE). The results showed that the average annual precipitation for Rawalpindi, Islamabad, and Murree will be decrease by 43.86 mm, 60.85 mm, and 86.86 mm, respectively, while average annual maximum temperature will be increased by 3.73 °C, 4.12 °C, and 1.33 °C, respectively, and average annual minimum temperature will be increased by 3.59 °C, 3.89 °C, and 2.33 °C, respectively, in early to late century under RCP-4.5 and RCP-8.5. Consequently, the average annual stream flow will be decreased in the future. According to the results, we found that it is possible to assess how CC will affect small water regions in the RCPs using small scale climate projections. Full article

Nanoparticles typically have dimensions of less than 100 nm. Scientists around the world have recently become interested in nanotechnology because of its potential applications in a wide range of fields, including catalysis, gas sensing, renewable energy, electronics, medicine, diagnostics, medication delivery, cosmetics, the construction industry, and the food industry. The sizes and forms of nanoparticles (NPs) are the primary determinants of their properties. Nanoparticles’ unique characteristics may be explored for use in electronics (transistors, LEDs, reusable catalysts), energy (oil recovery), medicine (imaging, tumor detection, drug administration), and more. For the aforementioned applications, the synthesis of nanoparticles with an appropriate size, structure, monodispersity, and morphology is essential. New procedures have been developed in nanotechnology that are safe for the environment and can be used to reliably create nanoparticles and nanomaterials. This research aims to illustrate top-down and bottom-up strategies for nanomaterial production, and numerous characterization methodologies, nanoparticle features, and sector-specific applications of nanotechnology. Full article

The adoption of mobile Internet technology (MIT) in organizational systems is rapidly increasing. MIT has developed agricultural structures and dramatically altered farming activities to improve agricultural systems. MIT is considered one of the most essential technologies because of its huge impact on agriculture, economy, and our daily lives. In this study, we utilized sample data from 460 wheat growers in the Khyber Pakhtunkhwa (KP) province of Pakistan to analyze the adoption of MIT and its impact on the promotion of wheat productivity. This study used the propensity score matching (PSM) technique to address probable self-selection bias. Existing research outcomes show that decision making, education, farm size, cooperative membership, gender, extension services, access to credit, weather forecast information, risk perception, market distance, and other factors significantly affect productivity and subsidy awareness agendas. However, outcomes signify that MIT adoption will have a significant positive impact on wheat productivity. This research concludes with a policy impact, emphasizing that it is essential to accelerate MIT adoption by wheat growers as a policy to enhance Pakistan’s agriculture or wheat productivity and food security. Full article

To overcome the issue of multidrug resistant (MDR) microbes, the exploration of ways to improve the antimicrobial efficiency of existing antibiotics is one of the promising approaches. In search of synthons with higher efficiency, in current investigations, cocrystal and amorphous salt of levofloxacin hemihydrate (LEV) were developed with phthalimide (PTH) and caffeic acid (CFA). New materials were characterized with the help of FT-IR, Raman spectroscopy, powder X-ray diffraction (PXRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Shifting, attenuation, appearance/disappearance and broadening of bands were observed in the FT-IR and Raman spectra of the materials as evidence of the required product. The PXRD diffraction pattern observed for LEV-PTH indicated cocrystal while halo diffractogram of LEV-CFA revealed amorphous nature. DSC/TG analysis confirmed the hydrated nature of the cocrystal/salt. The dissolution rate and antimicrobial activity against selected strains, K.pneumonia, E. coli and S. typhi of parent drug and the new material were compared. The zone of inhibition (ZI) observed for 5 µg LEV-PTH was 30.4 + 0.36 (K. pneumonia), 26.33 + 0.35 (E. coli) and 30.03 + 0.25 mm (S. typhi) while LEV-CFA salt (5 µg) against the same strains inhibited 33.96 ± 0.25, 31.66 ± 0.35 and 27.93 ± 0.40 mm, respectively. These novel formulations enhance the dissolution rate as well as antibacterial efficiency and are expected to be potent against MDR bacterial strains. Full article

A molecularly imprinting polymer (MIP) was synthesized for Basic Blue 3 dye and applied to wastewater for the adsorption of a target template. The MIPs were synthesized by bulk polymerization using methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA). Basic Blue 3 dye (BB-3), 2,2′-azobisisobutyronitrile (AIBN) and methanol were used as a functional monomer, cross linker, template, initiator and porogenic solvent, respectively, while non-imprinting polymers (NIP) were synthesized by the same procedure but without template molecules. The contact time was 25 min for the adsorption of BB-3 dye from 10 mL of spiked solution using 25 mg polymer. The adsorption of dye (BB-3) on the MIP followed the pseudo-second order kinetic (k₂ = 0.0079 mg·g⁻¹·min⁻¹), and it was according to the Langmuir isotherm, with maximum adsorption capacities of 78.13, 85.4 and 99.0 mg·g⁻¹ of the MIP at 283 K, 298 K and 313 K, respectively and 7 mg·g⁻¹ for the NIP. The negative values of ΔG° indicate that the removal of dye by the molecularly imprinting polymer and non-imprinting polymer is spontaneous, and the positive values of ΔH° and ΔS° indicate that the process is endothermic and occurred with the increase of randomness. The selectivity of the MIP for BB-3 dye was investigated in the presence of structurally similar as well as different dyes, but the MIP showed higher selectivity than the NIP. The imprinted polymer showed 96% rebinding capacity at 313 K towards the template, and the calculated imprinted factor and Kd value were 10.73 and 2.62, respectively. In this work, the MIP showed a greater potential of selectivity for the template from wastewater relative to the closely similar compounds. Full article

Due to its recalcitrant and carcinogenic nature, the presence of methyl orange (MO) in the environment is a serious threat to human and animal life and is also toxic to plants. MO being recalcitrant cannot be effectively reclaimed from industrial effluents through physical and chemical approaches. Biological methods on the other hand have the potential to degrade such dyes because of their compatibility with nature and low chances of adverse effects on the environment. Bacteria, due to their fast growth rate and capability of surviving in extreme environments can effectively be used for this purpose. In the current research study, Pseudomonas aeruginosa was isolated and characterized using 16rRNA from textile wastewater. In the preliminary tests it was found that Pseudomonas aeruginosa has the ability to degrade and mineralize methyl orange effectively. The physicochemical conditions were then optimized, in order to get maximum degradation of MO which was achieved at 37 °C, a pH of 7, a low salt concentration of 0.1 g/15 mL, a high carbon source of 0.6 g/15 mL, and 72 h experimental time. In a single set of experiments where all these optimum conditions were combined, 88.23% decolorization of the selected dye was achieved. At the end of the experimental cycle, the aliquots were homogenized and filtered. The filtrates were subjected to FTIR and GC-MS analysis where azo linkage breaking was confirmed from the FTIR spectra. The filtrates were then extracted with ethyl acetate and then passed through a silica gel column. On the basis of Rf value (TLC plates used) similar fraction were combined which were then subjected to NMR analysis. The compounds detected through GC-MS, peaks were not observed in proton and C-13 NMR. Instead, solvent and some impurity peaks were present, showing that complete mineralization of the dye had occurred due to the action of different bacterial enzymes such as azoreductase, peroxidases, and classes on MO. The prosed mechanism of complete mineralization is based on spectral data that needs to be verified by trapping the individual step products through the use of appropriate inhibitors of individual enzymes. Full article

The colossal depths of the deep neural network sometimes suffer from ineffective backpropagation of the gradients through all its depths, whereas the strong performance of shallower multilayer neural structures proves their ability to increase the gradient signals in the early stages of training, which easily gets backpropagated for global loss corrections. Shallow neural structures are always a good starting point for encouraging the sturdy feature characteristics of the input. In this research, a shallow, deep neural structure called PrimeNet is proposed. PrimeNet is aimed to dynamically identify and encourage the quality visual indicators from the input to be used by the subsequent deep network layers and increase the gradient signals in the lower stages of the training pipeline. In addition to this, the layer-wise training is performed with the help of locally generated errors, which means the gradient is not backpropagated to previous layers, and the hidden layer weights are updated during the forward pass, making this structure a backpropagation free variant. PrimeNet has obtained state-of-the-art results on various image datasets, attaining the dual objective of: (1) a compact dynamic deep neural structure, which (2) eliminates the problem of backward-locking. The PrimeNet unit is proposed as an alternative to traditional convolution and dense blocks for faster and memory-efficient training, outperforming previously reported results aimed at adaptive methods for parallel and multilayer deep neural systems. Full article

Lectins are unique glycoproteins that react with specific sugar residues on cell surfaces resulting in agglutination. They offer enormous applications in therapeutics, diagnostics, medicine, and agriculture. Rice lectins are naturally expressed during biotic and abiotic stresses suggesting their importance in stress resistance physiology. The objective of this study was to determine the presence and relative concentration of lectins in different accessions of rice obtained from IABGR/NARC Islamabad mainly originated from Pakistan. About 210 rice accessions including 02 local varieties and 05 transgenic seeds were screened for seed lectins using a hemagglutination (HA) assay with 5% Californian bred rabbits’ erythrocytes. A protein concentration of 3–8 mg/100 mg of seed flour was measured for all the rice accessions; the highest was 8.03 mg for accession 7600, while the lowest noted was 3.05 mg for accession 7753. Out of 210 accessions, 106 showed the highest HA activity. These 106 genotypes were further screened for titer analysis and specific activity. The highest titer and specific activity were observed for accession 7271 as 1024 and 236 hemagglutination unit (HAU), respectively. The selected accessions’ relative affinity and HA capability were evaluated using blood from four different sources: human, broiler chicken, local rabbit, and Californian-breed rabbit. The highest HA activity was observed with Californian-breed rabbit RBCs. The lectin assay was stable for about 1–2 h. After the required investigations, the accessions with higher lectin concentration and HA capability could be used as a readily available source of lectins for further characterization and utilization in crop improvement programs. Full article