Search Results (840)

Quinoa (Chenopodium quinoa), a stress-tolerant pseudocereal ideal for studying abiotic stress responses, was used to systematically identify optimal reference genes for qPCR normalization under gradient stresses: low temperatures (LT group: −2 °C to −10 °C), heat (HT group: 39° C to 45 °C), and drought (DR group: 7 to 13 days). Through multi-algorithm evaluation (GeNorm, NormFinder, BestKeeper, the ΔCt method, and RefFinder) of eleven candidates, condition-specific optimal genes were established as ACT16 (Actin), SAL92 (IT4 phosphatase-associated protein), SSU32 (Ssu72-like family protein), and TSB05 (Tryptophan synthase beta-subunit 2) for the LT group; ACT16 and NRP13 (Asparagine-rich protein) for the HT group; and ACT16, SKP27 (S-phase kinase), and NRP13 for the DR group, with ACT16, NRP13, WLIM96 (LIM domain-containing protein), SSU32, SKP27, SAL92, and UBC22 (ubiquitin-conjugating enzyme E2) demonstrating cross-stress stability (global group). DHDPS96 (dihydrodipicolinate synthase) and EF03 (translation elongation factor) showed minimal stability. Validation using stress-responsive markers—COR72 (LT), HSP44 (HT), COR413-PM (LT), and DREB12 (DR)—confirmed reliability; COR72 and COR413-PM exhibited oscillatory cold response patterns, HSP44 peaked at 43 °C before declining, and DREB12 showed progressive drought-induced upregulation. Crucially, normalization with unstable genes (DHDPS96 and EF03) distorted expression profiles. This work provides validated reference standards for quinoa transcriptomics under abiotic stresses. Full article

Background/Objectives: Chemotherapy-induced neuropathic pain (CINP) represents a critical challenge in oncology, emerging as a common and debilitating side effect of widely used chemotherapeutic agents, such as paclitaxel (PTX). Current therapeutic interventions and preventive strategies for CINP are largely insufficient, as they fail to address the underlying peripheral nerve damage, highlighting an urgent need for the development of new drugs. This study aimed to investigate the dual-function effects on normal cell protection and tumor suppression of BMX-001, a redox-active manganese metalloporphyrin that has demonstrated antioxidant and anti-inflammatory properties, which offers potential in protecting central nervous system tissues and treating CINP. Methods: This study assessed BMX-001’s different roles in protecting normal cells while acting as a pro-oxidant and pro-inflammatory molecule in cancer cells in vitro. We also evaluated its neuroprotective effect in preclinical PTX-induced CINP models in vivo. Results: Our results showed significant reductions in mechanical and cold allodynia, decreased pro-inflammatory cytokine levels, and restored antioxidant capacity in peripheral nerves and dorsal root ganglia (DRGs) following BMX-001 treatment. Conclusions: Overall, our study highlights the therapeutic potential of BMX-001 to mitigate CINP and enhance anticancer efficiency. Its dual-selective mechanism supports the future clinical investigation of BMX-001 as a novel adjunct to chemotherapeutic regimens. Full article

Consider a class of coupled discrete-time Riccati equations arising from jump systems. To compute their solutions when systems reach a steady state, we propose an operator Newton method and correspondingly establish its quadratic convergence under suitable assumptions. The advantage of the proposed method lies in the fact that its subproblems are solved using the operator Smith method, which allows it to maintain quadratic convergence in both the inner and outer iterations. Moreover, it does not require the constant term matrix of the equation to be invertible, making it more broadly applicable than existing inverse-free iterative methods. For large-scale problems, we develop a low-rank variant by incorporating truncation and compression techniques into the operator Newton framework. A complexity analysis is also provided to assess its scalability. Numerical experiments demonstrate that the presented low-rank operator Newton method is highly effective in approximating solutions to large-scale structured coupled Riccati equations. Full article

Visual object tracking is one of the core techniques in human-centered artificial intelligence, which is very useful for human–machine interaction. State-of-the-art tracking methods have shown their robustness and accuracy on many challenges. However, a large amount of videos with precisely dense annotations are required for fully supervised training of their models. Considering that annotating videos frame-by-frame is a labor- and time-consuming workload, reducing the reliance on manual annotations during the tracking models’ training is an important problem to be resolved. To make a trade-off between the annotating costs and the tracking performance, we propose a weakly supervised tracking method based on co-saliency learning, which can be flexibly integrated into various tracking frameworks to reduce annotation costs and further enhance the target representation in current search images. Since our method enables the model to explore valuable visual information from unlabeled frames, and calculate co-salient attention maps based on multiple frames, our weakly supervised methods can obtain competitive performance compared to fully supervised baseline trackers, using only 3.33% of manual annotations. We integrate our method into two CNN-based trackers and a Transformer-based tracker; extensive experiments on four general tracking benchmarks demonstrate the effectiveness of our method. Furthermore, we also demonstrate the advantages of our method on egocentric tracking task; our weakly supervised method obtains 0.538 success on TREK-150, which is superior to prior state-of-the-art fully supervised tracker by 7.7%. Full article

With the increasing severity of urban noise pollution, its detrimental impact on public health has garnered growing attention. However, accurate identification and classification of noise sources in complex urban acoustic environments remain major technical challenges for achieving refined noise management. To address this issue, this study presents two key contributions. First, we construct a new urban noise classification dataset, namely the urban noise 15-category dataset (UN15), which consists of 1620 audio clips from 15 representative categories, including traffic, construction, crowd activity, and commercial noise, recorded from diverse real-world urban scenes. Second, we propose a novel deep neural network architecture based on a residual network and integrated with a time–frequency attention mechanism, referred to as residual network with temporal–frequency attention (ResNet-TF). Extensive experiments conducted on the UN15 dataset demonstrate that ResNet-TF outperforms several mainstream baseline models in both classification accuracy and robustness. These results not only verify the effectiveness of the proposed attention mechanism but also establish the UN15 dataset as a valuable benchmark for future research in urban noise classification. Full article

Phosphogypsum, a by-product of phosphate fertilizer production, was predominantly used as a supplementary additive in recycled construction materials. However, there are few detailed studies on utilizing phosphogypsum as the primary component in inorganic cementing materials while achieving cost-effective detoxification. This study aimed to develop a harmless phosphogypsum-based inorganic cementing material (PICM) mainly based on phosphogypsum, in which cement, quicklime, and a stabilizer were used as additives. Harmful ions and acidity were first detected through X-ray fluorescence and ion chromatography and then harmlessly treated with quicklime. Compaction parameters, mechanical performance, X-ray diffraction analysis, moisture, and freezing resistance were characterized successively. The results illustrated that fluoride and phosphate ions were the primary soluble contaminants, whose leaching solution concentration can be reduced to 15.31 mg/L and undetectable with 2% quicklime through the mass proportion of phosphogypsum added and mixed. Meanwhile, the corresponding pH value was also raised to over 8. Cement content and quicklime were positively correlated with PICM’s maximum dry density. PICM with 25% cement and 2.5% stabilizer presented the highest unconfined compression strength, and flexural strength did not show significant regularity. PICM was mainly composed of quartz, gypsum, ettringite, and calcite, whose content decreased as cement content and quicklime content increased. Stabilizer, quicklime and cement content were positively correlated with PICM’s freezing and moisture resistance. Full article

The rapid evolution of pathogens and the limited genetic diversity of hosts are two major factors contributing to the plant pathogenic phenomenon known as the loss of disease resistance in maize (Zea mays L.). It has emerged as a significant biological stressor threatening the global food supplies and security. Based on previous cross-species homologous gene screening assays conducted in the laboratory, this study identified the maize disease-resistance candidate gene ZmNLR-7 to investigate the maize immune regulation mechanism against Bipolaris maydis. Subcellular localization assays confirmed that the ZmNLR-7 protein is localized in the plasma membrane and nucleus, and phylogenetic analysis revealed that it contains a conserved NB-ARC domain. Analysis of tissue expression patterns revealed that ZmNLR-7 was expressed in all maize tissues, with the highest expression level (5.11 times) exhibited in the leaves, and that its transcription level peaked at 11.92 times 48 h post Bipolaris maydis infection. Upon inoculating the ZmNLR-7 EMS mutants with Bipolaris maydis, the disease index was increased to 33.89 and 43.33, respectively, and the lesion expansion rate was higher than that in the wild type, indicating enhanced susceptibility to southern corn leaf blight. Physiological index measurements revealed a disturbance of ROS metabolism in ZmNLR-7 EMS mutants, with SOD activity decreased by approximately 30% and 55%, and POD activity decreased by 18% and 22%. Moreover, H₂O₂ content decreased, while lipid peroxide MDA accumulation increased. Transcriptomic analysis revealed a significant inhibition of the expression of the key genes NPR1 and ACS6 in the SA/ET signaling pathway and a decrease in the expression of disease-related genes ERF1 and PR1. This study established a new paradigm for the study of NLR protein-mediated plant immune mechanisms and provided target genes for molecular breeding of disease resistance in maize. Overall, these findings provide the first evidence that ZmNLR-7 confers resistance to southern corn leaf blight in maize by synergistically regulating ROS homeostasis, SA/ET signal transduction, and downstream defense gene expression networks. Full article

Hydraulic actuated legged robots display bright prospects and significant research value in areas such as unmanned area surveying, disaster rescue, military fields, and other scenarios owing to their excellent bionic characteristics, particularly their heavy payload capabilities and high power density. To realize the all-terrain adaptation locomotion of the hydraulic hexapod robot (HHR) with a heavy payload, one alternative control framework is position–posture control based on joint position control. As the foundation for the steady locomotion of HHRs, it is imperative to realize high-precision joint position control to improve the robustness under external disturbances during the walking process and to complete the attitude control task. To address the above issues, this paper proposes a sliding mode repetitive control based on the unknown dynamics estimator (SMRC + UDE) for the knee and hip joints of the HHR with a two-stage supply pressure hydraulic system (TSS). The effectiveness of the SMRC + UDE method is verified using a simulation environment and the ZJUHEX01 prototype experimental platform, and it is compared with the results for PID and adaptive robust sliding mode control (ARSMC). The results show that SMRC + UDE may be more suitable for our HHR, considering both the control performance and efficiency factors. Full article

Black rockfish (Sebastes schlegelii) is a marine ovoviviparous teleost that exhibits significant sexual dimorphism, with females growing faster and reaching larger sizes than males. Establishing stable oogonial stem cells (OSCs) is critical for understanding germline stem cell dynamics and facilitating all-female breeding. In this study, we successfully isolated and cultured OSCs from S. schlegelii for 12 passages. These cells exhibited alkaline phosphatase activity, expressed germline marker genes (ddx4, cdh1, klf4), and maintained a diploid karyotype (2n = 48). Transcriptomic comparisons between early (P3) and late (P12) passages revealed significant metabolic dysfunction and cell cycle arrest in the late-passage cells. Specifically, the down-regulation of glutathione-related and glycolysis-related genes (gstm3, gstt1, mgst3, gsta1, gsta4, gsto1, gapdh) and key mitotic regulators (cdk1, chk1, cdk4, e2f3, ccne2, ccnb1) suggested that metabolic imbalance contributes to oxidative stress, resulting in cell cycle inhibition and eventual senescence. This study provides a marine fish model for investigating metabolism-cell cycle interactions in germline stem cells and lays the foundation for future applications in germ cell transplantation and all-female breeding. Full article

The building sector holds a significant position in the global energy consumption share, and its environmental impact continues to intensify, making the construction industry a key player in sustainable development. The application of life cycle assessment on buildings (LCA-B) is widely employed to evaluate building energy and environment performance, and thus is of great significance for ensuring the sustainability of the project. This work aims to provide a systematic overview of LCA-B development based on reviewed literature. A three-stage mixed research method is adopted in this study: Firstly, an overall analysis framework is constructed, and 327 papers related to building life cycle assessment published between 2009 and 2025 are screened out by using the bibliometric method; Then, through scientometrics analysis, the journal regions, sources, scholars, and keyword evolution are revealed and analyzed using VOSviewer tool, and the hotspots in the field of LCA-B (e.g., integration of building information modeling (BIM) in LCA-B, multi-dimensional framework of environment–society–culture) are preliminarily explored based on the selected highly cited papers. The research finds that: (1) the performance of low energy buildings is better than that of net zero energy buildings from the perspective of LCA; (2) software compatibility and data exchange are the main obstacles in the integration of BIM-LCA; (3) a multi-dimensional LCA framework covering the social or cultural aspects is expected for a comprehensive assessment of building performance. This study provides a systematic analysis and elaboration of review articles related to LCA-B and thereby provides researchers with in-depth insight into this field. Full article

Multilayer diffractive optical elements (MLDOEs), which have broadband imaging performance, are widely used in lightweight and compact optical systems on the surface of the refractive lenses forming refractive–diffractive lenses. However, current research is generally limited to its broadband diffraction efficiency distribution and rarely involves the study of the imaging quality of multilayer refractive–diffractive optical elements in the broadband. The lack of research on its aberration principle and the absence of methods on how to achieve average aberration control in the broadband have led to a decline in imaging quality when it is applied to the optical system. Therefore, in this paper, we have derived in detail the aberration theory of multilayer refractive–diffractive optical elements and proposed the polychromatic integral aberration (PIA) method to evaluate the aberration characteristics of multilayer diffraction optical elements in the whole broadband. First, we start with the aberrations of diffractive optical elements in the air, and then derive the overall aberrations applied to multilayer refractive–diffractive optical elements. Then, based on the performance of the average aberrations throughout the entire broadband, a broadband aberration evaluation method named PIA is proposed. Finally, the design of traditional multilayer diffraction optical elements, the design of refractive–diffractive multilayer optical elements based on the derivation, and the design of multilayer refraction diffraction optical elements under the PIA method are compared. The results show that the multilayer refractive–diffractive optical element designed by PIA can effectively achieve aberration control and balanced aberration performance in the whole broadband. This research provides a practical and feasible path for exploring the imaging quality of broadband multilayer refractive–diffractive optical elements. Full article

Species characterized by undetermined clade affiliations, limited research coverage, and deficient systematic investigation serve as enigmatic entities in plant and animal taxonomy, yet hold critical significance for exploring phylogenetic relationships and evolutionary trajectories. Subgenus Cyathophora (Allium, Amayllidaceae), a small taxon comprising approximately five species distributed in the Qinghai–Tibet Plateau (QTP) and adjacent regions might contain an enigmatic species that has long remained unexplored. In this study, we collected data on species from subgenus Cyathophora and its close relatives in subgenus Rhizirideum, as well as the enigmatic species Allium siphonanthum. Combining phylogenomic datasets and morphological evidence, we investigated species relationships and the underlying mechanism of phylogenetic discordance. A total of 1662 single-copy genes (SCGs) and 150 plastid loci were filtered and used for phylogenetic analyses based on concatenated and coalescent-based methods. Furthermore, to systematically evaluate phylogenetic discordance and decipher its underlying drivers, we implemented integrative analyses using multiple approaches, such as coalescent simulation, Quartet Sampling (QS), and MSCquartets. Our phylogenetic analyses robustly resolve A. siphonanthum as a member of subg. Cyathophora, forming a sister clade with A. spicatum. This relationship was further corroborated by their shared morphological characteristics. Despite the robust phylogenies inferred, extensive phylogenetic conflicts were detected not only among gene trees but also between SCGs and plastid-derived species trees. These significant phylogenetic incongruences in subg. Cyathophora predominantly stem from incomplete lineage sorting (ILS) and reticulate evolutionary processes, with historical hybridization events likely correlated with the past orogenic dynamics and paleoclimatic oscillations in the QTP and adjacent regions. Our findings not only provide new insights into the phylogeny of subg. Cyathophora but also significantly enhance our understanding of the evolution of species in this subgenus. Full article

In this study, a terahertz microfluidic multi-band sensor was designed. Unlike previous microfluidic absorption sensors that rely on dipole resonance, the proposed sensor uses a physical mechanism for absorption by exciting higher-order lattice resonances in microfluidic structures. With a Fabry–Perot cavity, the sensor can form an absorption peak with a high quality factor (Q) and narrow full width at half maximum (FWHM). A high Q value and a narrow FWHM are valuable in the field of sensing and provide strong support for high-precision sensing. On this basis, the sensing performance of the device was investigated. The simulation results clearly show that the absorption sensor has ultra-high sensitivity, which reaches 400 GHz/Refractive Index Unit (RIU). In addition, the sensor generates three absorption peaks, overcoming the limitations of a single frequency band in a composite resonance mode and multidimensional frequency response, which has potential application value in the field of volatile organic compound (VOC) sensing. Full article

The effects of heavy traffic and complex natural environmental conditions have made the problem of the inadequate life expectancy of asphalt pavements increasingly pronounced. In this study, finite-element software was used to establish the three-dimensional analytical model of temperature-load coupling under different axial loads and calculate the distribution law of temperature-load coupling stress under the most unfavorable loading conditions. By comparing temperature and coupled stresses at different depths, the extent to which combined stress changes due to environmental factors affect different depths was determined. Finally, the fatigue life patterns of asphalt pavements under different seasons and axle loads were analyzed. The results showed that the temperature-load coupling stress varied periodically under different axial loads. Among them, the temperature stress had less influence on the coupling stress in spring and fall and more influence in winter. As the depth increases, the coupling stresses and their range of influence gradually decrease. Also, the farther away from the wheel load position, the smaller the traveling load disturbance and the closer the coupling stresses were to the temperature stresses. Under the most unfavorable loading conditions, the change rule of the degree of influence of environmental effects along the depth direction showed that the winter gradually decreased, the spring and fall seasons for the first time decreased and then increased, and the minimum influence on the road surface was at 9 cm. Overall, the degree of influence of environmental action at different axial loads was 70.53%, 41.90%, 27.13%, and 23.77% along the depth direction. Full article