Search Results (5)

Monitoring the state of oceans and their evolution in space and time is of fundamental importance as they are severely impacted by climate change, showing an increase in temperature, acidity and stratification. The role of metrology in the marine sector is relevant for helping oceanographers consolidate measurement approaches already in place by introducing concepts like metrological traceability and measurement uncertainty. The aim of this paper is to present some examples of successful and potential applications of metrology in oceanographic research, with a focus on past and ongoing activities in the framework of joint research cooperation, which could be applied by oceanographers to consolidate the comparability of data acquired in different experimental conditions, and places and time for some essential ocean variables. Scientific cooperation in the framework of joint research projects is particularly useful for supporting measurement capabilities in marine research worldwide, and the technologies and methods developed so far represent a starting point for improvements in international monitoring networks. These techniques may be applied by laboratories and centres working in the marine sector. Applications and possible future developments will also be discussed in this paper. Full article

Conjugation with the small ubiquitin-like modifier (SUMO) modulates protein interactions and localisation. The kinase Aurora B, a key regulator of mitosis, was previously identified as a SUMOylation target in vitro and in assays with overexpressed components. However, where and when this modification genuinely occurs in human cells was not ascertained. Here, we have developed intramolecular Proximity Ligation Assays (PLA) to visualise SUMO-conjugated Aurora B in human cells in situ. We visualised Aurora B-SUMO products at centromeres in prometaphase and metaphase, which declined from anaphase onwards and became virtually undetectable at cytokinesis. In the mitotic window in which Aurora B/SUMO products are abundant, Aurora B co-localised and interacted with NUP358/RANBP2, a nucleoporin with SUMO ligase and SUMO-stabilising activity. Indeed, in addition to the requirement for the previously identified PIAS3 SUMO ligase, we found that NUP358/RANBP2 is also implicated in Aurora B-SUMO PLA product formation and centromere localisation. In summary, SUMOylation marks a distinctive window of Aurora B functions at centromeres in prometaphase and metaphase while being dispensable for functions exerted in cytokinesis, and RANBP2 contributes to this control, adding a novel layer to modulation of Aurora B functions during mitosis. Full article

Radiosoundings are essential for weather and climate applications, as well as for calibration and validation of remote sensing observations. Vaisala RS92 radiosondes have been widely used on a global scale until 2016; although in the fall of 2013, Vaisala introduced the RS41 model to progressively replace the RS92. To ensure the highest quality and homogeneity of measurements following the transition from RS92 to RS41, intercomparisons of the two radiosonde models are needed. A methodology was introduced to simultaneously test and compare the two radiosonde models inside climatic chambers, in terms of noise, calibration accuracy, and bias in temperature measurements. A pair of RS41 and RS92 radiosondes has been tested at ambient pressure under very different temperature and humidity conditions, reproducing the atmospheric conditions that a radiosonde can meet at the ground before launch. The radiosondes have also been tested before and after fast (within ≈ 10 s) temperature changes of about ±20 °C, simulating a scenario similar to steep thermal changes that radiosondes can meet when passing from indoor to outdoor environment during the pre-launch phase. The results show that the temperature sensor of RS41 is less affected by noise and more accurate than that of RS92, with noise values less than 0.06 °C for RS41 and less than 0.1 °C for RS92. The deviation from the reference value, referred to as calibration error, is within ±0.1 °C for RS41 and the related uncertainty (hereafter with coverage factor k = 1) is less than 0.06 °C, while RS92 is affected by a cold bias in the calibration, which ranges from 0.1 °C up to a few tenths of a degree, with a calibration uncertainty less than 0.1 °C. The temperature bias between RS41 and RS92 is within ±0.1 °C, while its uncertainty is less than 0.1 °C. The fast and steep thermal changes that radiosondes can meet during the pre-launch phase might lead to a noise increase in temperature sensors during radiosoundings, up to 0.1 °C for RS41 and up to 0.3 °C for RS92, with a similar increase in their calibration uncertainty, as well as an increase in the uncertainty of their bias up to 0.3 °C. Full article

Characterization of dynamics inside clouds remains a challenging task for weather forecasting and climate modeling as cloud properties depend on interdependent natural processes at micro- and macro-scales. Turbulence plays an important role in particle dynamics inside clouds; however, turbulence mechanisms are not yet fully understood partly due to the difficulty of measuring clouds at the smallest scales. To address these knowledge gaps, an experimental method for measuring the influence of small-scale turbulence in cloud formation in situ and producing an in-field cloud Lagrangian dataset is being developed by means of innovative ultralight radioprobes. This paper presents the electronic system design along with the obtained results from laboratory and field experiments regarding these compact (diameter ≈30 cm), lightweight (≈20 g), and expendable devices designed to passively float and track small-scale turbulence fluctuations inside warm clouds. The fully customized mini-radioprobe board (5 cm × 5 cm) embeds sensors to measure local fluctuations and transmit data to the ground in near real time. The tests confirm that the newly developed probes perform well, providing accurate information about atmospheric turbulence as referenced in space. The integration of multiple radioprobes allows for a systematic and accurate monitoring of atmospheric turbulence and its impact on cloud formation. Full article

Kinetochores are large protein assemblies that connect chromosomes to microtubules of the mitotic and meiotic spindles in order to distribute the replicated genome from a mother cell to its daughters. Kinetochores also control feedback mechanisms responsible for the correction of incorrect microtubule attachments, and for the coordination of chromosome attachment with cell cycle progression. Finally, kinetochores contribute to their own preservation, across generations, at the specific chromosomal loci devoted to host them, the centromeres. They achieve this in most species by exploiting an epigenetic, DNA-sequence-independent mechanism; notable exceptions are budding yeasts where a specific sequence is associated with centromere function. In the last 15 years, extensive progress in the elucidation of the composition of the kinetochore and the identification of various physical and functional modules within its substructure has led to a much deeper molecular understanding of kinetochore organization and the origins of its functional output. Here, we provide a broad summary of this progress, focusing primarily on kinetochores of humans and budding yeast, while highlighting work from other models, and present important unresolved questions for future studies. Full article