Methylpiperidinopyrazole Attenuates Estrogen-Induced Mitochondrial Energy Production and Subsequent Osteoblast Maturation via an Estrogen Receptor Alpha-Dependent Mechanism
Abstract
:1. Introduction
2. Results
2.1. MPP Did Not Induce Cytotoxicity in Rat Calvarial Osteoblasts
2.2. MPP Diminished Estradiol-Induced Translocation of ERα from the Cytoplasm To Mitochondria in Rat Calvarial Osteoblasts
2.3. MPP Concurrently Inhibited Estradiol-Induced Expressions of Mitochondrial Energy Production-Linked COX I and COX II mRNAs in Rat Calvarial Osteoblasts
2.4. MPP Subsequently Lowered Estradiol-Triggered Enhancements of Mitochondrial Complex Enzyme Activities and Cellular ATP Levels in Rat Calvarial Osteoblasts
2.5. MPP Accordingly Inhibited Estradiol-Induced Expressions of Osteoblast Maturation-Associated BMP-6 and Type I Collagen mRNAs in Rat Calvarial Osteoblasts
2.6. MPP Lowered Estradiol-Induced Activation and Mineralization of Rat Calvarial Osteoblasts
3. Discussion
4. Materials and Methods
4.1. Preparation of Rat Osteoblasts
4.2. Drug Treatment
4.3. Analyses of Cell Morphology and Cell Survival
4.4. Preparation of Mitochondrial Proteins
4.5. Immunodetection of Mitochondrial ERα Protein
4.6. Real-Time Polymerase Chain Reaction (PCR)
4.7. Assay of Mitochondrial Enzyme Activity
4.8. Measurement of Cellular ATP
4.9. Examination of Osteoblast Mineralization
4.10. Analysis of ALP Activity
4.11. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Sample of the compound methylpiperidinopyrazole is available from the authors. |
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Yeh, P.-S.; Chen, J.-T.; Cherng, Y.-G.; Yang, S.-T.; Tai, Y.-T.; Chen, R.-M. Methylpiperidinopyrazole Attenuates Estrogen-Induced Mitochondrial Energy Production and Subsequent Osteoblast Maturation via an Estrogen Receptor Alpha-Dependent Mechanism. Molecules 2020, 25, 2876. https://doi.org/10.3390/molecules25122876
Yeh P-S, Chen J-T, Cherng Y-G, Yang S-T, Tai Y-T, Chen R-M. Methylpiperidinopyrazole Attenuates Estrogen-Induced Mitochondrial Energy Production and Subsequent Osteoblast Maturation via an Estrogen Receptor Alpha-Dependent Mechanism. Molecules. 2020; 25(12):2876. https://doi.org/10.3390/molecules25122876
Chicago/Turabian StyleYeh, Poh-Shiow, Jui-Tai Chen, Yih-Giun Cherng, Shun-Tai Yang, Yu-Ting Tai, and Ruei-Ming Chen. 2020. "Methylpiperidinopyrazole Attenuates Estrogen-Induced Mitochondrial Energy Production and Subsequent Osteoblast Maturation via an Estrogen Receptor Alpha-Dependent Mechanism" Molecules 25, no. 12: 2876. https://doi.org/10.3390/molecules25122876