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Separations
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Trends in Modern High Performance Liquid Chromatography Separations
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Trends in Modern High Performance Liquid Chromatography Separations

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (31 May 2019) | Viewed by 4272

Special Issue Editor

Dr. Serban C. Moldoveanu

E-Mail Website
Guest Editor
R.J. Reynolds Tobacco Co., Winston-Salem, NC 27105, USA
Interests: HPLC; GC; MS; pyrolysis; sample preparation

Special Issue Information

Dear Colleagues,

High performance liquid chromatography (HPLC) is very likely the most utilized technique in analytical chemistry and it sees a continuous progress. This progress is significant, with novel column technologies, new instruments development including a variety of new detection techniques mainly based on mass spectrometry, as well as numerous new applications. The goal of this Special Issue is to publish further results reflecting the progress in modern HPLC. This may include original research or reviews related to the use of special HPLC columns (sub 2 mm particles, superficially porous particles, organic polymeric columns, bio-inert columns, monolithic columns, micro-fabricated columns, etc.), special HPLC and UHPLC instrumentation, as well as new applications of HPLC and UHPLC. These applications may include the use of new types of columns or instrumentation, but also methods for ultra trace analyses, biological samples analysis, or any new method applying HPLC for solving difficult analytical problems. Results regarding new theoretical developments in HPLC will also be considered.

I would like to invite colleagues to contribute to this special issue with any kind of original results or reviews covering latest trends in HPLC.

Dr. Serban C. Moldoveanu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • HPLC
  • UHPLC
  • LC/MS special
  • HPLC columns modern
  • HPLC instrumentation trace analysis

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Published Papers (1 paper)

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Research

8 pages, 1178 KiB  
Article
To Develop the Method for UHPLC-HRMS to Determine the Antibacterial Potential of a Central American Medicinal Plant
by Gaganpreet Kaur Monga, Anima Ghosal and Dil Ramanathan
Separations 2019, 6(3), 37; https://doi.org/10.3390/separations6030037 - 29 Jul 2019
Cited by 4 | Viewed by 3787
Abstract
The development of antibiotic resistance by microbials has long been acknowledged. The major challenge worldwide is to develop novel, natural, and potent antibiotics against the multidrug resistant bacteria. In this study, our aim was to develop the method for a highly sensitive instrument, [...] Read more.
The development of antibiotic resistance by microbials has long been acknowledged. The major challenge worldwide is to develop novel, natural, and potent antibiotics against the multidrug resistant bacteria. In this study, our aim was to develop the method for a highly sensitive instrument, ultra-high performance liquid chromatograph-high resolution mass spectrometer (UHPLC-HRMS), to evaluate the antibacterial property of a natural product. Aechmea magdalenae (Andre) Andre ex Baker, a plant belonging to the family Bromeliaceae, a native of Central America was used in this study. Based on the available literature, it was hypothesized that Aechmea magdalenae has antibacterial activity. In addition, the profiling done on A. magdalenae using gas chromatography-mass spectrometry (GC-MS) also revealed the presence of medicinally important chemical compounds, such as acetic acid. Minimum inhibitory concentration (MIC) of dried Aechmea plant extract was determined for the first time using 96-well plate assay, followed by determination of antibacterial potential using LC-MS. The reason being that other dried methanolic plant extracts, such as Vismia macrophylla, lined up for antibacterial testing have dark extracts, for which determining the antibacterial potential and reading the results with the naked eye would be challenging. To overcome the situation of dark plant extracts, a generalized novel LC-MS method was developed that was used for the plant A. magdalenae, and would be used further for other plants. A blue indicator called resazurin was added to the wells; resazurin, upon incubation with the living cells, got reduced to resorufin (which was pink), while it remained blue with bacterial growth inhibition. The mass difference created due to reduction of resazurin to resorufin was detected by using LTQ Orbitrap Discovery in positive ion mode to determine the antibacterial activity of the plant extract. The sample preparation for LC-MS assay included centrifugation of the samples taken from 96-well plate, followed by filtration of the supernatant, before exposing them to C-18 column. The results obtained from full scan LC-MS spectrum consistently demonstrated the presence of resorufin from wells with bacterial growth, and resazurin from wells with inhibition through peaks of relevant masses. Full article
(This article belongs to the Special Issue Trends in Modern High Performance Liquid Chromatography Separations)
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