Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
2.5
2.6
Journals
Separations
Special Issues
Fractionation and Sample Preparation Techniques Used in Bioanalyses
share announcement

Fractionation and Sample Preparation Techniques Used in Bioanalyses

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Bioanalysis/Clinical Analysis".

Deadline for manuscript submissions: closed (15 June 2022) | Viewed by 2950

Special Issue Editor

Dr. Eszter Simon

E-Mail Website
Guest Editor
Federal Office for the Environment (FOEN), Bern, Switzerland
Interests: in vitro bioassays; endocrine disruption; effect-directed analysis; sample preparation; environmental water analysis

Special Issue Information

Dear Colleagues,

Sample preparation is key. Whether performing bioanalysis on biological samples themselves (e.g., tissues, homogenates, blood samples) or on extracts of various environmental compartments (e.g., water, soil, sediment, suspended matters, passive samplers), sample preparation predetermines the success of subsequent analysis. Organic and inorganic matrix components, such as lipids, proteins, salts, and humic acids can interfere with analysis by masking the biological effects of interest. Fractionation techniques can strengthen and complement bioanalyses by separating target analytes from such matrix components, providing improved detection of bioactive substances and better chances of identification.

The Special Issue welcomes any developments in sample preparation and/or fractionation techniques that enhance, specify, and are beneficial for the bioanalysis of biotic and abiotic samples.

Dr. Eszter Simon
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioassay
  • effect-based methods
  • separation
  • extraction
  • cleanup

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

14 pages, 2316 KiB  
Article
Alternative Method for HDL and Exosome Isolation with Small Serum Volumes and Their Characterizations
by Rian Ka Praja, Wisitsak Phoksawat, Patcharaporn Tippayawat, Amonrat Jumnainsong and Chanvit Leelayuwat
Separations 2021, 8(11), 204; https://doi.org/10.3390/separations8110204 - 03 Nov 2021
Cited by 3 | Viewed by 2329
Abstract
High-density lipoprotein (HDL) and exosomes are promising sources of biomarkers. However, the limited sample volume and access to the ultracentrifuge equipment are still an issue during HDL and exosome isolation. This study aimed to isolate HDL and exosomes using an ultracentrifugation-free method with [...] Read more.
High-density lipoprotein (HDL) and exosomes are promising sources of biomarkers. However, the limited sample volume and access to the ultracentrifuge equipment are still an issue during HDL and exosome isolation. This study aimed to isolate HDL and exosomes using an ultracentrifugation-free method with various small serum volumes. HDL was isolated from 200 µL (HDL200) and 500 µL (HDL500) of sera. Three different volumes: 50 µL (Exo50), 100 µL (Exo100), and 250 µL (Exo250) were used for exosome isolation. HDL and exosomes were isolated using commercial kits with the modified method and characterized by multiple approaches. The HDL levels of HDL200 and HDL500 were not significantly different (p > 0.05), with percent recoveries of >90%. HDL200 and HDL500 had the same protein pattern with a biochemical similarity of 99.60 ± 0.10%. The particle sizes of Exo50, Exo100, and Exo250 were in the expected range. All isolated exosomes exhibited a similar protein pattern with a biochemical similarity of >99%. In conclusion, two different serum volumes (200 and 500 µL) and three different serum volumes (50, 100, and 250 µL) can be employed for HDL and exosome isolation, respectively. The possibility of HDL and exosome isolation with small volumes will accelerate biomarker discoveries with various molecular diagnostic approaches. Full article
(This article belongs to the Special Issue Fractionation and Sample Preparation Techniques Used in Bioanalyses)
►▼ Show Figures

Graphical abstract

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop