Application of Microbial Metallurgy Process in Metal Extraction

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Purification Technology".

Deadline for manuscript submissions: closed (30 April 2024) | Viewed by 1311

Special Issue Editors


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Guest Editor
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China
Interests: biometallurgy and environmental microbiology; omics, molecular biology, structural biology; minerals processing and mine pollution remediation; biosensor and environmental monitoring

Special Issue Information

Dear Colleagues,

Biometallurgy is a technology that extracts valuable metals from ores through the use of microorganisms. In this process, ores are decomposed under the actions of microorganisms, so that the valuable metals in the minerals are dissolved into or exposed onto solution, and further extracted using various physical and chemical methods, e.g., liquid-phase extraction, electrodeposition, adsorption. This technology has been successfully applied in metal extraction in a dozen of metals, e.g., Cu, Au, U, Zn, Ag, Ni, Co, Sn, Sb, and become one of the most important industrial methods of processing low-grade complex refractory ores. The environments of the minerals bioleaching are usually extremely acid and full of various toxic metal ions in very high concentrations; thus, the microorganisms that survive in these environments are considered a miracle of life and possess many peculiar abilities and properties. The microorganisms and these genes and proteins that they harbor are also precious resources for various applications’ developments, e.g., cell biosensors, Taq DNA polymerase enzyme. Therefore, studies in this field are of both great interest and great application significance.

We invite you to contribute to this Special Issue dedicated to biometallurgy and all its relevant aspects, e.g., minerals bioleaching, microorganisms, community structure and function, genes and proteins, microbe–mineral interface, solution reactions, metals extraction, mechanism and techniques, are in consideration.

Dr. Yuandong Liu
Prof. Dr. Ruiyong Zhang
Guest Editors

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Keywords

  • minerals bioleaching
  • microbe–mineral interface
  • solution reactions
  • microorganisms
  • community structure and function
  • genes and proteins
  • multiple bio-omics
  • bioleaching mechanism
  • biometallurgy techniques
  • new applications development

Published Papers (1 paper)

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Research

16 pages, 2688 KiB  
Article
Expression, Purification and Characterization of a Novel Rusticyanin from the Psychrotolerant Acidithiobacillus ferrivorans
by Yuandong Liu, Lixiang Chen, Xiangdong Shangguan, Jingying Ouyang, Jiayu He, Kan Wang, Yan Tong, Runlan Yu, Weimin Zeng, Xueling Wu, Li Shen and Guanzhou Qiu
Separations 2023, 10(8), 448; https://doi.org/10.3390/separations10080448 - 13 Aug 2023
Cited by 1 | Viewed by 959
Abstract
Rusticyanin plays a crucial role in ferrous oxidation of sulfide minerals during bioleaching for industrial metal extraction. Diverse isoforms of rusticyanin have been found, but until now, except for type-A rusticyanin, other isoforms or sources of rusticyanin have been scarcely investigated. Here, a [...] Read more.
Rusticyanin plays a crucial role in ferrous oxidation of sulfide minerals during bioleaching for industrial metal extraction. Diverse isoforms of rusticyanin have been found, but until now, except for type-A rusticyanin, other isoforms or sources of rusticyanin have been scarcely investigated. Here, a rusticyanin (gene locus 0470) from the psychrophilic Acidithiobacillus ferrivorans was gene-cloned, expressed, purified, and assembled in vitro. All forms of the protein exhibit extreme acid stability, even at pH 0.3. The stability of the protein is obviously enhanced after binding of the copper cofactor; the oxidation state is more stable than the reduced state. The protein has characteristic UV-vis peaks and EPR signals similar to type-A or type-B rusticyanin but is different with a small position shift and an obvious intensity change. The vibrational spectrum of the apoprotein was more different than these between the oxidation and reduced states of the protein. The ferrous oxidation kinetic rate constant of the protein is obviously faster than that of both the type-A and type-B rusticyanins previously reported. Further bioinformatics analysis reveal their changes in sequence and molecule: the mutations related to the peculiar shield belt mostly account for the variation in the properties of the protein, and the classification of the protein as a new isoform, type-C rusticyanin, is proposed. Full article
(This article belongs to the Special Issue Application of Microbial Metallurgy Process in Metal Extraction)
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