Special Issue "New Frontier in Mass Spectrometry Imaging for Metabolomics and Lipidomics"

A special issue of Metabolites (ISSN 2218-1989). This special issue belongs to the section "Metabolomic Profiling Technology".

Deadline for manuscript submissions: 31 January 2023 | Viewed by 1892

Special Issue Editors

Prof. Dr. Shuichi Shimma
E-Mail Website
Guest Editor
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan
Interests: mass spectrometry imaging; metabolomics; target and nontarget analysis
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Eiichiro Fukusaki
E-Mail Website
Co-Guest Editor
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan
Interests: metabolomics; food; microorganisms; fermentation; sensory evaluation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Since the first report in 1997, mass spectrometry imaging (MSI) has been applied in various fields, including medicine and basic biology, due to the development of techniques for sample preparation, analysis, and informatics for data processing; it is no exaggeration to say that it has become one of the main fields of molecular imaging. This Special Issue provides an overview of current MSI, including basic and applied research on sample preparation methods for MSI in metabolomics and lipidomics and new measurement methods using state-of-the-art instruments. Therefore, it will cover applied studies conducting MSI on various samples such as human, animal, and cultured cells, as well as bacteria; this can be in the context of, but is not limited to, the application of metabolomics and lipidomics methods in medicine or biotechnology, data mining methods based on nontargeted analytical data, and high-sensitivity quantitative methods in MSI. Manuscripts addressing other relevant and challenging problems are also strongly encouraged.

Prof. Dr. Shuichi Shimma
Prof. Dr. Eiichiro Fukusaki
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Metabolites is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • metabolomics
  • lipidomics
  • medical and biological applications
  • sample preparation
  • target analysis
  • nontarget analysis
  • data mining

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Article
Visualization of Glutamate Decarboxylase Activity in Barley Seeds under Salinity Stress Using Mass Microscope
Metabolites 2022, 12(12), 1262; https://doi.org/10.3390/metabo12121262 - 14 Dec 2022
Viewed by 432
Abstract
γ-Aminobutyric acid (GABA) accumulates in plants in response to environmental stresses. The activity levels of glutamate decarboxylase (GAD), an enzyme involved in GABA biosynthesis, are reported to increase during germination under salinity stress. However, it is not clear which tissues of the plant [...] Read more.
γ-Aminobutyric acid (GABA) accumulates in plants in response to environmental stresses. The activity levels of glutamate decarboxylase (GAD), an enzyme involved in GABA biosynthesis, are reported to increase during germination under salinity stress. However, it is not clear which tissues of the plant seeds are affected by GAD activity in response to salinity stress. In this study, the effects of salinity stress on the distribution of barley seeds GAD activity during germination were investigated. The mass spectrometry imaging (MSI) method was optimized, and the distribution of GAD activity in germinated seeds exposed to salinity stress at different germination stages from 12 to 48 h after imbibition was investigated. In this study, MSI was successfully applied to enzyme histochemistry to visualize the relative GAD activity in germinating barley seeds for the first time. The salinity stress increased the GAD activity, mostly due to the increase in relative GAD activity in the embryo. Higher GAD activity was detected in seeds exposed to salinity stress in the scutellum or aleurone layer, which are difficult to separate for extraction. This method can be used to clarify the role of GABA shunts, including GAD enzyme responses, in barley seeds under stress. Full article
Show Figures

Graphical abstract

Article
MALDI Mass Spectrometry Imaging Reveals the Existence of an N-Acyl-homoserine Lactone Quorum Sensing System in Pseudomonas putida Biofilms
Metabolites 2022, 12(11), 1148; https://doi.org/10.3390/metabo12111148 - 21 Nov 2022
Viewed by 671
Abstract
Quorum sensing (QS) is generally used to describe the process involving the release and recognition of signaling molecules, such as N-acyl-homoserine lactones, by bacteria to coordinate their response to population density and biofilm development. However, detailed information on the heterogeneity of QS [...] Read more.
Quorum sensing (QS) is generally used to describe the process involving the release and recognition of signaling molecules, such as N-acyl-homoserine lactones, by bacteria to coordinate their response to population density and biofilm development. However, detailed information on the heterogeneity of QS metabolites in biofilms remains largely unknown. Here, we describe the utilization of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to follow the production of specific metabolites, including QS metabolites, during Pseudomonas putida biofilm development. To do so, a method to grow an agar-based biofilm was first established, and MALDI-MSI was used to detect and visualize the distribution of QS metabolites in biofilms at different cultivation times. This study demonstrated that N-acyl-homoserine lactones are homogeneously produced in the early stages of P. putida biofilm formation. In contrast, the spatial distribution of quinolones and pyochelin correlated with the swarming motility of P. putida in mature biofilms. These two metabolites are involved in the production of extracellular polymeric substances and iron chelators. Our study thus contributes to establishing the specific temporal regulation and spatial distribution of N-acyl-homoserine lactone-related metabolites and quinolone and pyochelin in P. putida biofilms. Full article
Show Figures

Figure 1

Back to TopTop