Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
4.4
4.2
Journals
Membranes
Special Issues
Nanoscale Membranes Structure and Dynamics
share announcement

Nanoscale Membranes Structure and Dynamics

A special issue of Membranes (ISSN 2077-0375). This special issue belongs to the section "Inorganic Membranes".

Deadline for manuscript submissions: closed (31 August 2021) | Viewed by 13348

Special Issue Editors

Dr. Silvia Galiani

E-Mail Website
Guest Editor
MRC Human Immunology Unit and Wolfson Imaging Centre, MRC Weatherall Institute of Molecular Medicine, University of Oxford, Headley Way, Oxford OX3 9DS, UK
Interests: fluorescence microscopy; STED microscopy; advanced microscopy techniques in living cells; development of bespoken microscopy
Special Issues, Collections and Topics in MDPI journals
Dr. Kseniya Korobchevskaya

E-Mail Website
Guest Editor
Biophysical Immunology Lab, Kennedy Institute of Rheumatology, University of Oxford, Oxford OX3 7FY, UK
Interests: super-resolution microscopy; structured illumination microscopy; fluorescence microscopy; image analysis; high spatial–temporal resolution microscopy for life sciences
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Cellular membranes are a heterogeneous and complex architecture of domains, clusters and nanostructures that constitute one of the main hubs of cellular signaling. This generates a wide interest and a strong motivation to disclose nanoscale structures and dynamics of cellular membranes. Advanced microscopy techniques have successfully opened the pathway to resolve the molecular organization and nanoscale mobility of the cells at a yet unseen level of detail.

This Special Issue on “Nanoscale Membranes Structure and Dynamics” of the journal Membranes seeks contributions to assess state-of-the-art and future developments in the field of advanced techniques to disclose membrane structures and dynamics. Topics include, but are not limited to super-resolution microscopy techniques, model systems, spectroscopy techniques, and new dye development. Authors are invited to submit their latest results; both original papers and reviews are welcome.

Dr. Silvia Galiani
Dr. Kseniya Korobchevskaya
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Membranes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (3 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

14 pages, 2619 KiB  
Article
Quantitative Super-Resolution Microscopy to Assess Adhesion of Neuronal Cells on Single-Layer Graphene Substrates
by Silvia Scalisi, Francesca Pennacchietti, Sandeep Keshavan, Nathan D. Derr, Alberto Diaspro, Dario Pisignano, Agnieszka Pierzynska-Mach, Silvia Dante and Francesca Cella Zanacchi
Membranes 2021, 11(11), 878; https://doi.org/10.3390/membranes11110878 - 15 Nov 2021
Cited by 3 | Viewed by 2427
Abstract
Single Layer Graphene (SLG) has emerged as a critically important nanomaterial due to its unique optical and electrical properties and has become a potential candidate for biomedical applications, biosensors, and tissue engineering. Due to its intrinsic 2D nature, SLG is an ideal surface [...] Read more.
Single Layer Graphene (SLG) has emerged as a critically important nanomaterial due to its unique optical and electrical properties and has become a potential candidate for biomedical applications, biosensors, and tissue engineering. Due to its intrinsic 2D nature, SLG is an ideal surface for the development of large-area biosensors and, due to its biocompatibility, can be easily exploited as a substrate for cell growth. The cellular response to SLG has been addressed in different studies with high cellular affinity for graphene often detected. Still, little is known about the molecular mechanism that drives/regulates the cellular adhesion and migration on SLG and SLG-coated interfaces with respect to other substrates. Within this scenario, we used quantitative super-resolution microscopy based on single-molecule localization to study the molecular distribution of adhesion proteins at the nanoscale level in cells growing on SLG and glass. In order to reveal the molecular mechanisms underlying the higher affinity of biological samples on SLG, we exploited stochastic optical reconstruction microscopy (STORM) imaging and cluster analysis, quantifying the super-resolution localization of the adhesion protein vinculin in neurons and clearly highlighting substrate-related correlations. Additionally, a comparison with an epithelial cell line (Chinese Hamster Ovary) revealed a cell dependent mechanism of interaction with SLG. Full article
(This article belongs to the Special Issue Nanoscale Membranes Structure and Dynamics)
►▼ Show Figures

Figure 1

Review

Jump to: Research

14 pages, 2145 KiB  
Review
How Does Liquid-Liquid Phase Separation in Model Membranes Reflect Cell Membrane Heterogeneity?
by Taras Sych, Cenk Onur Gurdap, Linda Wedemann and Erdinc Sezgin
Membranes 2021, 11(5), 323; https://doi.org/10.3390/membranes11050323 - 28 Apr 2021
Cited by 28 | Viewed by 7326
Abstract
Although liquid–liquid phase separation of cytoplasmic or nuclear components in cells has been a major focus in cell biology, it is only recently that the principle of phase separation has been a long-standing concept and extensively studied in biomembranes. Membrane phase separation has [...] Read more.
Although liquid–liquid phase separation of cytoplasmic or nuclear components in cells has been a major focus in cell biology, it is only recently that the principle of phase separation has been a long-standing concept and extensively studied in biomembranes. Membrane phase separation has been reconstituted in simplified model systems, and its detailed physicochemical principles, including essential phase diagrams, have been extensively explored. These model membrane systems have proven very useful to study the heterogeneity in cellular membranes, however, concerns have been raised about how reliably they can represent native membranes. In this review, we will discuss how phase-separated membrane systems can mimic cellular membranes and where they fail to reflect the native cell membrane heterogeneity. We also include a few humble suggestions on which phase-separated systems should be used for certain applications, and which interpretations should be avoided to prevent unreliable conclusions. Full article
(This article belongs to the Special Issue Nanoscale Membranes Structure and Dynamics)
►▼ Show Figures

Figure 1

16 pages, 2225 KiB  
Review
What Can Mushroom Proteins Teach Us about Lipid Rafts?
by Maja Grundner, Anastasija Panevska, Kristina Sepčić and Matej Skočaj
Membranes 2021, 11(4), 264; https://doi.org/10.3390/membranes11040264 - 6 Apr 2021
Cited by 10 | Viewed by 2935
Abstract
The lipid raft hypothesis emerged as a need to explain the lateral organization and behavior of lipids in the environment of biological membranes. The idea, that lipids segregate in biological membranes to form liquid-disordered and liquid-ordered states, was faced with a challenge: to [...] Read more.
The lipid raft hypothesis emerged as a need to explain the lateral organization and behavior of lipids in the environment of biological membranes. The idea, that lipids segregate in biological membranes to form liquid-disordered and liquid-ordered states, was faced with a challenge: to show that lipid-ordered domains, enriched in sphingomyelin and cholesterol, actually exist in vivo. A great deal of indirect evidence and the use of lipid-binding probes supported this idea, but there was a lack of tools to demonstrate the existence of such domains in living cells. A whole new toolbox had to be invented to biochemically characterize lipid rafts and to define how they are involved in several cellular functions. A potential solution came from basic biochemical experiments in the late 1970s, showing that some mushroom extracts exert hemolytic activities. These activities were later assigned to aegerolysin-based sphingomyelin/cholesterol-specific cytolytic protein complexes. Recently, six sphingomyelin/cholesterol binding proteins from different mushrooms have been identified and have provided some insight into the nature of sphingomyelin/cholesterol-rich domains in living vertebrate cells. In this review, we dissect the accumulated knowledge and introduce the mushroom lipid raft binding proteins as molecules of choice to study the dynamics and origins of these liquid-ordered domains in mammalian cells. Full article
(This article belongs to the Special Issue Nanoscale Membranes Structure and Dynamics)
►▼ Show Figures

Graphical abstract

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-3
Back to TopTop