Recent Developments in Optical Imaging

Dear Colleagues,

Recent developments in optical imaging have revolutionized our understanding of biological systems, offering unprecedented insights into cellular structures, dynamics, and functions. This Special Issue of J.Imaging focuses on highlighting cutting-edge advancements in optical imaging techniques and methodologies.

Super-resolution microscopy, including techniques such as STED, DNA-PAINT, and Single-Molecule Localization Microscopy (SMLM), has pushed the boundaries of spatial resolution, enabling the visualization of subcellular structures with nanometer-scale precision. Spatial omics approaches integrate high-resolution imaging with molecular profiling, facilitating the spatial mapping of biomolecules within cellular environments.

Time-lapse imaging and spatio-temporal imaging capture dynamic processes in real-time, providing valuable insights into cellular behaviors and interactions. Multi-photon microscopy and deep tissue imaging techniques penetrate thick samples, allowing for in vivo studies of biological processes.

Organoids, three-dimensional cell culture models, offer physiological relevance for studying developmental processes and disease mechanisms. Label-free imaging techniques, adaptive optics, and multiplex imaging methodologies further enhance the capabilities of optical imaging, preserving native cellular environments and enabling simultaneous visualization of multiple targets.

SMART microscopy focuses on high-resolution, multiplexed imaging, while event-driven microscopy selectively captures dynamic events in real-time. These emerging technologies provide novel insights into complex biological phenomena, offering new avenues for understanding cellular processes and disease mechanisms.

This Special Issue welcomes contributions that showcase recent developments in optical imaging, including novel imaging technologies, applications across various biological systems, and computational methods for data analysis. Research articles, reviews, and methodological advancements are invited for submission to highlight the latest innovations in the field of optical imaging and their implications for advancing our understanding of biology and medicine.

By bringing together diverse perspectives and cutting-edge research in optical imaging, this Special Issue aims to foster interdisciplinary collaboration and drive further advancements in the field. Contributions from researchers across disciplines are encouraged to contribute to this exciting endeavor in advancing optical imaging techniques and applications.

Keywords:

• multiplex optical imaging;
• adaptive optics;
• single molecule localisation microscopy;
• spatial omics;
• time-lapse imaging;
• multi-photon microscopy;
• TIRF;
• FCS/;FLIM;
• SRRF;
• hyperspectral imaging;
• volumetric imaging;
• correlative microscopy;
• intravital imaging;
• light sheet imaging;
• super-resolution imaging;
• image formation;
• optical design;
• organoids;
• label-free techniques;
• spatio-temporal imaging;
• dynamic imaging;
• multiplex imaging;
• SMART microscopy;
• event-driven microscopy;
• biomedical visualisation;
• computational analysis (AI, KI);
• holographic microscopy and tomography;
• image restoration

Dr. Ralf Palmisano
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Imaging is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

• multiplex optical imaging
• adaptive optics
• single molecule localization microscopy
• spatial omics
• multi-photon microscopy
• correlative microscopy
• intravital imaging
• light sheet imaging
• super-resolution imaging
• optical design
• spatio-temporal imaging
• SMART microscopy
• event-driven microscopy
• biomedical visualization
• holographic microscopy and tomography