Molecular Biology and Genetics in Aquaculture

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Animal Genetics and Genomics".

Deadline for manuscript submissions: closed (25 December 2024) | Viewed by 2799

Special Issue Editor


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Guest Editor
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
Interests: molecular biology; evolution biology; comparative immunology; genetics and genomics

Special Issue Information

Dear Colleagues,

The application of molecular biology and genetics is the key to promoting the healthy and sustainable development of aquaculture. In recent years, molecular biology and genetics have found successful applications in the study of genetic breeding, disease diagnosis, gene editing, genetic diversity protection, and nutrition and feeding science in aquaculture. This Special Issue, entitled “Molecular Biology and Genetics in Aquaculture”, aims to collect high-quality research articles, review articles, and communications focused on advances in the research area of molecular biology and genetics in aquaculture. Since the aim of this Special Issue is to illustrate, through selected works, advances in the research of molecular biology and genetics in aquaculture, we sincerely invite contributions to this Special Issue from relevant experts and colleagues. Topics of interest include, but are not limited to, the following:

Genetic breeding;

Disease diagnosis;

Gene editing;

Genetic diversity protection;

Comparative immunology;

Gene function and regulation;

Expression and evolution of gene families;

Molecular mechanisms of sex determination and differentiation.

Dr. Xiaojun Song
Guest Editor

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Keywords

  • molecular biology
  • genetics and genomics
  • evolution biology
  • regulatory network
  • aquaculture

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Published Papers (2 papers)

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Research

17 pages, 8313 KiB  
Article
Exposure to High Concentrations of Tetrabromobisphenol A Slows the Process of Tissue Regeneration and Induces an Imbalance of Metabolic Homeostasis in the Regenerated Intestines of Apostichopus japonicus
by Zi Wang, Xiaojun Song, Wenhui Yin, Kuntao Shi, Ying Lin, Jixiang Liu, Xiaohan Li, Jiabo Tan, Junjie Rong, Kefeng Xu and Guodong Wang
Genes 2024, 15(11), 1448; https://doi.org/10.3390/genes15111448 - 9 Nov 2024
Cited by 1 | Viewed by 1055
Abstract
Background: Tissue regenerative capacity following evisceration, potentially influenced by environmental contaminants and intestinal microflora, is essential for the financial success of Apostichopus japonicus farming. However, the morphological structure, gut microbiome composition, and genes expression pattern of the regenerated gut after exposure to high [...] Read more.
Background: Tissue regenerative capacity following evisceration, potentially influenced by environmental contaminants and intestinal microflora, is essential for the financial success of Apostichopus japonicus farming. However, the morphological structure, gut microbiome composition, and genes expression pattern of the regenerated gut after exposure to high levels of TBBPA remain poorly unclear. Methods: In this research, the effect of TBBPA exposure on tissue regeneration in A. japonicus was investigated through a comprehensive multi-omics approach. Results: Our results showed that the integrity, the intestinal wall thickness, and the villi length of the regenerated intestines in A. japonicus decreased after treatment with high levels of TBBPA. The findings from PCoA and NMDS analyses revealed that the microbial community composition was significantly altered following exposure to high concentrations of TBBPA in the regenerated intestines of A. japonicus. The KEGG pathway enrichment analysis indicated that the DEGs (differentially expressed genes) were predominantly enriched on metabolism and immunity-related signaling pathways after exposure to high levels of TBBPA. These included pathways involved in the PPAR signaling pathway, ECM receptor interaction, glycerolipid metabolism, and fatty acid degradation. Interestingly, the results have demonstrated that there are 77 transcript factors that were significantly different after exposure to TBBPA. Conclusions: These results suggested that high levels of exposure to TBBPA induces an imbalance of the metabolic homeostasis by regulating the expression levels of transcription factors in the regenerated intestines of A. japonicus. Full article
(This article belongs to the Special Issue Molecular Biology and Genetics in Aquaculture)
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18 pages, 2999 KiB  
Article
Next-Generation Sequencing to Determine Changes in the Intestinal Microbiome of Juvenile Sturgeon Hybrid (Acipenser gueldenstaedtii♀ × Acipenser baerii♂) Resulting from Sodium Butyrate, Β-Glucan and Vitamin Supplementation
by Martyna Arciuch-Rutkowska, Joanna Nowosad, Michał Krzysztof Łuczyński, Syed Makhdoom Hussain and Dariusz Kucharczyk
Genes 2024, 15(10), 1276; https://doi.org/10.3390/genes15101276 - 28 Sep 2024
Cited by 4 | Viewed by 1279
Abstract
Background/Objectives: The effect of sodium butyrate (NaB), β-glucan (βG) and vitamins in the diet on gut microbiome, cortisol level, lysozyme activity and growth parameters of juvenile hybrid sturgeon (Acipenser gueldenstaedtii♀ × Acipenser baerii♂) was determined. Methods: Sturgeon hybrids (n [...] Read more.
Background/Objectives: The effect of sodium butyrate (NaB), β-glucan (βG) and vitamins in the diet on gut microbiome, cortisol level, lysozyme activity and growth parameters of juvenile hybrid sturgeon (Acipenser gueldenstaedtii♀ × Acipenser baerii♂) was determined. Methods: Sturgeon hybrids (n = 144) were divided into three groups with enriched feeding (mg/kg of feed): FQV1 (50 NaB; 20 βG; const. vitamins), FQV2 (150 NaB; 20 βG; const. vitamins), FQV3 (50 NaB; 60 βG; const. vitamins) and control (not supplemented), each group in triplicate, 12 fish in each repetition. Rearing was carried out for 30 days in controlled conditions. Gut microbiome was characterized using Next Generation Sequencing (NGS) of DNA samples isolated from intestinal content. Cortisol level was determined using the ELISA test. Lysozyme activity was measured by turbidimetric test. Results: Based on data obtained from NGS, it was determined that the FQV1 group is characterized by the highest values of diversity indices (Shannon, Simpson and Chao-1) and the largest number of ASVs (Amplicon Sequence Variants). The highest abundance of probiotic bacteria (Lactobacillus, Lactococcus) was determined in the FQV1 group. The highest cortisol concentration was determined in the control (33.26 ng/mL), while the lowest was in FQV3 (27.75 ng/mL). The highest lysozyme activity was observed in FQV1 (154.64 U/mL), and the lowest in FQV2 (104.39 U/mL) and control (121.37 U/mL) (p < 0.05). FQV2 was characterized by significantly more favorable values of breeding indicators (p < 0.05). Conclusions: The obtained results prove that an appropriate composition of NaB, βG and vitamins can be used in the commercial breeding of juvenile hybrid sturgeons. Full article
(This article belongs to the Special Issue Molecular Biology and Genetics in Aquaculture)
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