Protein Delivery In Vitro or In Vivo

A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Biomedical Materials and Nanomedicine".

Deadline for manuscript submissions: closed (31 August 2020) | Viewed by 3102

Special Issue Editor


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Guest Editor
College of Pharmacy, Ajou University, Gyeonggi-do 16499, Korea
Interests: drug/gene/protein delivery nano-carrier system; magnetic nanoparticle; nanoliposome; microbubble; in vivo imaging; theranostics

Special Issue Information

Dear Colleagues,

Many attempts at protein delivery are being made in the field of biotechnology, due to the developments in drug development, molecular science, and material engineering. This can be used to identify the role of organelles in cells, the role of various biomaterials made from the nucleus, and can sometimes be a way to control cell death and growth. In general, due to the size and solubility of proteins, it is not easy to deliver them into cells or organs, and many researchers have tried to overcome this by developing various nano-carrier systems. Approaches to the delivery of peptides or genes pose challenges such as integration, short lifetime, and easy degradation. Recently, nano-carrier systems with organic and inorganic components have been developed, and efforts are being made to deliver various protein hybrids to cells or desired organs using them. This Special Issue focuses on the recent advances in protein delivery using nano-carrier systems in vitro or in vivo. The goal is to stimulate the related research and contribute to the development of various drugs, the study of mechanisms for cell growth inhibition, and the development of material engineering science.

Prof. Dr. Tae-Jong Yoon
Guest Editor

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Keywords

  • protein delivery
  • nanoparticles
  • targets
  • material sciences
  • encapsulation
  • hybrid protein complex delivery

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Published Papers (1 paper)

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Research

19 pages, 3529 KiB  
Article
Amphiphilic QP(DMAEMA-co-LMA)-b-POEGMA Random-Block Terpolymers as Nanocarriers for Insulin
by Martha Kafetzi, Stergios Pispas, Xiaoyan Bao and Ping Yao
Biomedicines 2020, 8(10), 392; https://doi.org/10.3390/biomedicines8100392 - 4 Oct 2020
Cited by 11 | Viewed by 2685
Abstract
We report on the utilization of the amphiphilic poly[quaternized (2-(N,N-dimethylamino) ethyl methacrylate)]-co-(lauryl methacrylate))-b-poly[(oligo ethylene glycol) methyl ether methacrylate] QP(DMAEMA-co-LMA)-b-POEGMA cationic diblock terpolymer aggregates as nanocarriers for insulin delivery applications. QP(DMAEMA-co-LMA)-b [...] Read more.
We report on the utilization of the amphiphilic poly[quaternized (2-(N,N-dimethylamino) ethyl methacrylate)]-co-(lauryl methacrylate))-b-poly[(oligo ethylene glycol) methyl ether methacrylate] QP(DMAEMA-co-LMA)-b-POEGMA cationic diblock terpolymer aggregates as nanocarriers for insulin delivery applications. QP(DMAEMA-co-LMA)-b-POEGMA random diblock terpolymer is derived from the chemical modification of the precursor amino diblock copolymer via quaternization, producing permanent positive charges on the macromolecular chain. The QP(DMAEMA-co-LMA)-b-POEGMA diblock terpolymer as well as its amino precursor investigated self-assemble in aqueous media, forming aggregates. In vitro cytotoxicity and in vivo biocompatibility studies on QP(DMAEMA-co-LMA)-b-POEGMA and its amino precursor aggregates, showed good cytocompatibility and biocompatibility. QP(DMAEMA-co-LMA)-b-POEGMA aggregates were chosen to be complexed with insulin due to their self-assembly features and the permanent positive charge in each amino group. QP(DMAEMA-co-LMA)-b-POEGMA aggregates were complexed with insulin through electrostatic interactions. Light scattering techniques were used in order to study the ability of the polymer aggregates to complex with insulin, to determine critical physicochemical parameters such as size, mass, and surface charge of the stable complexes and study the effect of salt addition on their properties. The results showed that in both cases, the complexation process was successful and as the insulin concentration increases, nanosized complexes of different physicochemical characteristics (mass, size, surface charge) and spherical morphology are formed. UV-Vis and fluorescence spectroscopy studies showed that no conformational changes of insulin occurred after the complexation. Full article
(This article belongs to the Special Issue Protein Delivery In Vitro or In Vivo)
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