Rickettsial Agents Associated with Ectoparasites in Attica, Greece
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
3. Results
4. Discussion
4.1. Rickettsia Felis
4.2. Rickettsia Conorii
4.3. Ehrlichia canis
4.4. Wolbachia pipientis
4.5. Candidatus Midichloria mitochondrii
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Target Species | Target Gene | PCR Primer and Probe Sequences (5′–3′) | Product Size (bp) | Reference |
---|---|---|---|---|
SFG Rickettsia genus specific | gltA | F: GTG AAT GAA AGA TTA CAC TAT TTAT | 166 | [23] |
R: GTA TCT TAG CAA TCA TTC TAA TAG C | ||||
FAM-CTA TTA TGC TTG CGG CTG TCG GTT C-TAMRA | ||||
Rickettsia conorii | Putative acetyltransferase | F: TTG GTA GGC AAG TAG CTA AGC AAA | 116 | [23] |
R: GGA AGT ATA TGG GAA TGC TTT GAA | ||||
FAM-GCG GTT ATT CCT GAA AAT AAG CCG GCA-TAMRA | ||||
Rickettsia felis specific | Biotin synthase | F: ATG-TTC-GGG-CTTCCG-GTA-TG | 120 | [21] |
R: CCG-ATT-CAG-CAGGTT-CTT-CAA | ||||
6-FAM- GCT-GCG-GCGGTA-TTT-TAG-GAA-TGGG-TAMRA | ||||
Anaplasmataceae | 16S rRNA | EHR16SD: GGT ACC YAC AGA AGA AGTCC | 345 | [17] |
EHR16SR: TAG CAC TCA TCG TTT ACAGC | ||||
PanWolbachia | 23S rRNA | F: CCA AAA TTA CAG CTA AGT GG | 100 | [19] |
R: AGT GAG CTG TTA CGC TTT CT 6FAM-TACAGCTAGGAGGTTGGCTT-TAMRA | ||||
Wolbachia pipientis | rpoB | F: TCATGCGCGTTCAGTAGGAC | 103 | Unpublished |
R: TGCCCAACATTCCATTTCAC 6FAM-AGGAAAGTCTCATTTTGGTGGTAGCG-TAMRA |
Rickettsial Agent | Pathogen/ Endosymbiont | Prevalence (%) | Examined Ectoparasite | Method of Detection | Sequence Identity (%) | Query Cover (%) | GenBank ID |
---|---|---|---|---|---|---|---|
Anaplasmataceae | Ehrlichia canis | 2.4% | R. sanguineus s.l. | PCR | 100% (305/305 bp) | 100% | MN922610 |
Ca. Midichloria mitochondrii | 2.4% | Ixodes sp. | PCR | 100% (305/305 bp) | 100% | KX359181 | |
Wolbachia pipientis | 58.7% a | C. felis | PCR | 100% (305/305 bp) | 100% | CP051156 | |
Wolbachia pipientis | 61.5% | C. felis | qPCR | na b | na b | ||
SFG Rickettsiae | Rickettsia conorii | 7.3% | R. sanguineus s.l. | qPCR | na b | na b | |
Rickettsia felis | 4.8% | C. felis | qPCR | na b | na b |
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Liodaki, M.; Angelakis, E.; Spanakos, G.; Papadogiannaki, I.; Samarkos, M.; Daikos, G.L.; Christopoulou, B.; Piperaki, E.-T. Rickettsial Agents Associated with Ectoparasites in Attica, Greece. Zoonotic Dis. 2022, 2, 247-257. https://doi.org/10.3390/zoonoticdis2040020
Liodaki M, Angelakis E, Spanakos G, Papadogiannaki I, Samarkos M, Daikos GL, Christopoulou B, Piperaki E-T. Rickettsial Agents Associated with Ectoparasites in Attica, Greece. Zoonotic Diseases. 2022; 2(4):247-257. https://doi.org/10.3390/zoonoticdis2040020
Chicago/Turabian StyleLiodaki, Maria, Emmanouil Angelakis, Gregory Spanakos, Ioanna Papadogiannaki, Michael Samarkos, George L. Daikos, Barbara Christopoulou, and Evangelia-Theophano Piperaki. 2022. "Rickettsial Agents Associated with Ectoparasites in Attica, Greece" Zoonotic Diseases 2, no. 4: 247-257. https://doi.org/10.3390/zoonoticdis2040020