Seeds, Volume 5, Issue 2 (April 2026) – 7 articles

Rose hip processing generates seed-rich by-products that remain underexplored beyond oil extraction, despite their potential as a source of phenolic compounds and antioxidant activity. This study investigates the effect of bioprocessing (short-term fermentation) on the phenolic composition and antioxidant activity of rose hip (Rosa spp.) seed by-products, with relevance to cosmetic-oriented applications related to oxidative stress modulation. Rose hip seeds were obtained after juice production and subjected to short-term fermentation (14 days at 21 °C) using Saccharomyces cerevisiae, followed by mechanical separation and drying. Non-fermented and bioprocessed seeds were analyzed for individual phenolic compounds and antioxidant activity (DPPH, ABTS, FRAP), and correlation and multivariate analyses were conducted. Bioprocessing reduced total identified phenolics from 15.79 to 10.72 mg/g DW (≈32%), primarily due to a decrease in epigallocatechin (10.89 to 6.50 mg/g DW). In parallel, the relative contribution of phenolic acids increased, including gallic acid (0.50 to 0.60 mg/g DW) and salicylic acid (0.98 to 1.20 mg/g DW), indicating a selective compositional redistribution accompanied by partial degradation. Antioxidant activity decreased after bioprocessing (DPPH ~340 to ~250 µmol TE/g DW) but remained substantial. Correlation analysis identified epigallocatechin as the main contributor to antioxidant capacity. These findings show that rose hip seeds behave as a process-sensitive phenolic matrix in which bioprocessing alters the balance of individual compounds without complete loss of antioxidant activity. The results indicate that seed-derived by-products retain functional potential for further valorization in cosmetic-oriented applications. Full article

Durum wheat (Triticum turgidum L. var. durum) plays a central role in global food and nutritional security, yet its grain mineral and protein quality remain highly variable across varieties and environments. This study aimed to evaluate the combined effects of varieties, nitrogen (N) fertilization, foliar zinc (Zn) and sulfur (S) application, and seasonal variation on grain macro- and micronutrients (Ca, Mg, K, Zn) and protein content, adopting a system-level approach that integrates varieties, nutrient management, and seasonal variability. We hypothesized that aligning variety selection with precision nutrient management would improve grain nutritional quality while enhancing resilience to environmental variation. Significant differences among varieties (p < 0.05) were observed, with the spring-sown variety Durablank exhibiting the highest grain Zn concentration (34.70 mg kg⁻¹) compared with MV Pelsodur (23.0 mg kg⁻¹) and GK Julidur (23.8 mg kg⁻¹), representing a 50.87% varietal difference. Grain Ca, Mg, and K varied widely across varieties and seasons, with drought in 2022 increasing grain Zn, while the wetter 2023 season enhanced Mg and K accumulation. A clear yield–protein trade-off was found, where high-yielding MV Pelsodur maintained 14.3% protein while moderate-yielding Durablank reached 16.8%. Foliar Zn and S applications significantly increased grain Zn, particularly in Zn-efficient varieties. Nitrogen fertilization at 100 kg ha⁻¹ improved macronutrient accumulation by 12–20% compared with 60 kg N ha⁻¹. Significant varieties × N × season interactions (p < 0.01) highlight the need for adjusted varieties -specific nutrient management strategies. Overall, the findings demonstrate that integrating genetic selection with optimized fertilization and season-responsive management practices may contribute to improving grain nutritional quality of durum wheat. Full article

The collection of high-quality seeds to produce forest seedlings is closely linked with the time of harvesting. Climate warming is already having visible effects in all life stages of forest tree species, including the timing of seed maturation. The purpose of this study was to update the knowledge on seed collection timing and to identify the indicators of physiological maturity for three key Eastern European tree species—silver fir (Abies alba), European larch (Larix decidua), and small-leaved lime (Tilia cordata). Seeds and cones were collected from Romanian clonal seed orchards and evaluated at several stages of seed maturation using germination tests for European larch and tetrazolium viability tests for silver fir and small-leaved lime. The results revealed species-specific differences in seed maturation timing: in silver fir seed viability increased slightly from late August to early September, in European larch germination remained low (≈20%) regardless of harvest time, while small-leaved lime viability declined significantly after late August. These findings suggest that the harvest period observed during the study years occurred earlier than the traditionally recommended intervals and could be linked to recent warming trends. This study highlights the relevance of re-evaluating seed collection schedules under changing climatic conditions, while further multi-year studies are required to confirm these patterns and refine practical recommendations. Full article

Viviparity is rarely reported in species with seed physical dormancy. This is because physical dormancy, common in tropical trees, results from the impermeability of the seed tegument to water and regulates the timing of germination. Records of germination within the fruit in such species suggest greater functional plasticity than traditionally assumed. This study reports and quantifies viviparity in Hymenaea martiana seeds over four consecutive years (2019–2022) and assessed seed dormancy status through vigor tests. Sixty fruits were collected annually, and viviparity was estimated as the proportion of fruits containing at least one seed with radicle protrusion. Non-viviparous seeds were subjected to emergence tests with and without mechanical scarification, and cumulative emergence, percentages of dead and water-impermeable seeds, and seed water content were evaluated. Viviparity occurred consistently, affecting approximately 10–15% of the fruits, with no significant differences between years, indicating that the phenomenon was not associated with extreme environmental events. However, early germination did not result in seedling establishment because the emerging roots dehydrated while still inside the fruit. Mechanical scarification significantly increased emergence, confirming the presence of seed physical dormancy. These results show that, although viviparity was observed recurrently in the studied population of H. martiana, seed physical dormancy remains the main mechanism controlling germination in this species. Full article

The objective of this research was to apply morphometric and chemometric techniques to analyze the influence of climate and soil type on the morphological, proximate, and fatty acid fingerprints of moringa (Moringa oleifera Lam.) seeds cultivated in different regions of Mexico. Seeds were collected from the states of Chiapas, Michoacán, Nuevo León, Oaxaca, Veracruz, and Yucatán. The morphological traits of the seeds were evaluated, while the proximate composition and fatty acid profiles of the seed flours were analyzed using gas chromatography–mass spectrometry (GC–MS). Data were assessed through analysis of variance (ANOVA) and linear discriminant analysis to develop their fingerprint profiles. The results showed that the morphological variables that constituted the climate-based morphological fingerprint were seed length, width, seed weight, and kernel weight, whereas for the soil type-based fingerprint, only seed length was significant. Regarding the proximate chemical composition, all variables (fat, ash, moisture, and protein), except fiber content, were influenced by both climate and soil type, forming the proximate chemical fingerprint. The fatty acid fingerprint consisted of 21 compounds, with oleic, behenic, stearic, palmitic, and arachidic acids present in the highest concentrations. The fingerprints obtained from the different determinations were confirmed through cross-validation values exceeding 50%, according to the linear discriminant analysis validation technique. The fatty acid and proximate composition determinations showed the highest classification values (83–100%) and contributed most significantly to ensuring the fingerprinting of moringa seeds cultivated in Mexico. Full article

Seed dispersal by humans plays an important role in determining vegetation structure. The seeds of Asian plantain (Plantago asiatica L.) form adhesive mucilage upon hydration, facilitating their attachment to shoes and subsequent dispersal via epizoochory. We investigated the efficacy of this mechanism under various urban environmental conditions. After trampling wild P. asiatica stands, the number of seeds attached to shoe soles was counted. The remaining seeds were then counted after walking at designated distances (1, 2, 5, 10, 20, 50, 100, 200, 500, and 1000 m). The following results were obtained: (1) The retention rate after walking 1000 m varied by shoe type (slip-on (kakkusu) work shoes, 15.4%; leather shoes, 3.4%; rubber boots, 2.7%; running shoes, 13.5%; and sandals, 12.4%). (2) Within the first 50 m of walking, on average more than half of the attached seeds fell off under all investigated conditions. Significantly fewer seeds remained after walking 50 m on asphalt (30.9% of the initial seeds) than on grass (48.2%), whereas after walking 1000 m, similar proportions (15.4% on asphalt and 15.7% on grass) remained on the work shoes. These results indicate that human-mediated short- and long-distance dispersal of mucilaginous seeds of this species is effective in diverse urban environments. Full article

Moringa oleifera (Lam.) is a multipurpose agroforestry tree cultivated worldwide for its nutritional, medicinal, and economic value, and it is increasingly grown commercially in subtropical regions, including Nepal. While vegetative propagation is feasible, large-scale production relies predominantly on seeds, making efficient germination critical for seedling establishment, uniform growth, sustainable production, and preservation of genetic diversity. Seed pre-treatments are widely recognized as a simple and effective approach to enhance germination, early seedling vigor, and nursery performance. This study evaluated the effects of seven pre-sowing treatments under controlled nursery conditions to determine the most effective method for improving Moringa oleifera seedling production. A total of 2100 seeds were used, with 100 seeds per treatment and three replicates, arranged in a Completely Randomized Design (CRD). Treatments included control (no pretreatment), normal water soaking (12 h and 24 h), alternating wetting (water) and drying cycles (12 h each), hot water soaking (60 °C for 5 min), cow urine soaking (1:2 of urine to water proportions for 12 h), and hydrochloric acid soaking (35% for 20 min). All pre-treatments were conducted at room temperature, and the seeds were subsequently sown in controlled nursery conditions. Seed germination was monitored twice daily for 30 days, and data were analyzed using one-way ANOVA and Tukey’s HSD test to identify significant differences in germination performances. Results demonstrated that alternating wetting and drying produced the highest germination percentage (89%), shortest mean germination time (8.44 days), and strongest seedling vigor, outperforming all other treatments. Conversely, cow urine and acid treatments completely inhibited germination. This study recommends alternating wetting and drying as a simple, low-cost, and chemical-free pre-treatment to optimize Moringa oleifera seedling production in nurseries. These findings provide practical guidance for commercial and smallholder farmers, contributing to sustainable agroforestry, food security, and climate-resilient livelihoods in resource-limited habitats. Full article