A B-Box Transcription Factor CoBBX24 from Camellia oleifera Delays Leaf Senescence and Enhances Drought Tolerance in Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatments
2.2. RNA Extraction and Real-Time Quantitative Polymerase Chain Reaction (qPCR)
2.3. Isolation and Sequence Analysis of CoBBX24
2.4. Subcellular Localization Analysis of CoBBX24
2.5. Transactivation Activity Analysis of CoBBX24
2.6. Transformation of Arabidopsis
2.7. ABA Treatment of Transgenic Arabidopsis
2.8. Determination of Chlorophyll Content
2.9. Statistical Analysis
3. Results
3.1. Cloning and Sequence Analysis of CoBBX24
3.2. Subcellular Localization, Transcriptional Activation, and Transcriptional Profiling of CoBBX24
3.3. Overexpression of CoBBX24 Confers Drought Tolerance in Arabidopsis
3.4. Overexpression of CoBBX24 in Arabidopsis Improves ABA Sensitivity
3.5. Overexpression of CoBBX24 Reduces ABA-Induced Leaf Senescence in Arabidopsis
3.6. CoBBX24 Regulates the Transcription of SAGs and CCGs in Arabidopsis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Liu, Y.; Zhu, Z.; Wu, Y.; Gao, Y.; Zhang, L.; Yu, C.; Ye, S.; Liu, W. A B-Box Transcription Factor CoBBX24 from Camellia oleifera Delays Leaf Senescence and Enhances Drought Tolerance in Arabidopsis. Horticulturae 2023, 9, 991. https://doi.org/10.3390/horticulturae9090991
Liu Y, Zhu Z, Wu Y, Gao Y, Zhang L, Yu C, Ye S, Liu W. A B-Box Transcription Factor CoBBX24 from Camellia oleifera Delays Leaf Senescence and Enhances Drought Tolerance in Arabidopsis. Horticulturae. 2023; 9(9):991. https://doi.org/10.3390/horticulturae9090991
Chicago/Turabian StyleLiu, Yanan, Zhiguo Zhu, Yang Wu, Yinxiang Gao, Lisha Zhang, Changshuai Yu, Sicheng Ye, and Wenxin Liu. 2023. "A B-Box Transcription Factor CoBBX24 from Camellia oleifera Delays Leaf Senescence and Enhances Drought Tolerance in Arabidopsis" Horticulturae 9, no. 9: 991. https://doi.org/10.3390/horticulturae9090991