Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics

Nashiruddin, Noor Idayu; Abd Rahman, Noor Hidayah; A. Rahman, Roshanida; Md. Illias, Rosli; Ghazali, Nazlee Faisal; Abomoelak, Bassam; El Enshasy, Hesham Ali

doi:10.3390/fermentation8110640

Open AccessArticle

Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics

by

Noor Idayu Nashiruddin

¹,

Noor Hidayah Abd Rahman

^1,*,

Roshanida A. Rahman

^1,2,*,

Rosli Md. Illias

^1,2,

Nazlee Faisal Ghazali

¹,

Bassam Abomoelak

³ and

Hesham Ali El Enshasy

^1,2,4

¹

Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Skudai 81310, Malaysia

²

Institute of Bioproduct Development, Universiti Teknologi Malaysia, Skudai 81310, Malaysia

³

Arnold Palmer Pediatric Gastroenterology Clinic, Orlando Health, Orlando, FL 32806, USA

⁴

City of Scientific Research and Technology Applications (SRTA), New Burg Al-Arab, Alexandria 21934, Egypt

^*

Authors to whom correspondence should be addressed.

Fermentation 2022, 8(11), 640; https://doi.org/10.3390/fermentation8110640

Submission received: 19 September 2022 / Revised: 1 November 2022 / Accepted: 11 November 2022 / Published: 14 November 2022

(This article belongs to the Special Issue Functional Foods and Probiotic Food Development: Trends, Concepts, and Products)

Download

Browse Figures

Versions Notes

Abstract

The biofibre complex structure in pineapple leaf fibres (PALFs) can be disrupted using the alkaline pre-treatment method with sodium hydroxide (NaOH). Nonetheless, the pre-treated structures have an impact on the bioconversion of PALFs into sugar. Thus, the impact necessitates precise reaction conditions, which are required for the enzymatic hydrolysis of PALFs. In this study, the Box–Behnken design (BBD) was utilised to achieve maximum sugar yield from PALFs. The optimised enzymatic hydrolysis conditions were 229 µg/mL of cellulase, a reaction temperature of 45 °C, and a hydrolysis time of 69 h. Resultantly, an 84% increase in sugar yield was observed, from 17.26 mg/mL to 108.74 mg/mL after the optimisation process. Moreover, a significant influence (p = 0.0009) of the enzymatic hydrolysis on hydrolysis time and temperature was observed. Additionally, the kinetic study analysis of NaOH pre-treatment and the enzymatic hydrolysis process were investigated. The kinetics of enzyme concentrations on total reducing sugar (TRS) production using an insoluble substrate were investigated based on modified Michaelis–Menten and Chrastil models. The result of the models is in good agreement with the experimental data, as the PALF hydrolysis process was reasonably well predicted. This study provides valuable information for predicting the sugar yield of the PALF bioconversion.

Keywords: pineapple leaf fibres; enzymatic hydrolysis; reducing sugar; Michaelis–Menten; Chrastil model

Share and Cite

MDPI and ACS Style

Nashiruddin, N.I.; Abd Rahman, N.H.; A. Rahman, R.; Md. Illias, R.; Ghazali, N.F.; Abomoelak, B.; El Enshasy, H.A. Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics. Fermentation 2022, 8, 640. https://doi.org/10.3390/fermentation8110640

AMA Style

Nashiruddin NI, Abd Rahman NH, A. Rahman R, Md. Illias R, Ghazali NF, Abomoelak B, El Enshasy HA. Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics. Fermentation. 2022; 8(11):640. https://doi.org/10.3390/fermentation8110640

Chicago/Turabian Style

Nashiruddin, Noor Idayu, Noor Hidayah Abd Rahman, Roshanida A. Rahman, Rosli Md. Illias, Nazlee Faisal Ghazali, Bassam Abomoelak, and Hesham Ali El Enshasy. 2022. "Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics" Fermentation 8, no. 11: 640. https://doi.org/10.3390/fermentation8110640

APA Style

Nashiruddin, N. I., Abd Rahman, N. H., A. Rahman, R., Md. Illias, R., Ghazali, N. F., Abomoelak, B., & El Enshasy, H. A. (2022). Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics. Fermentation, 8(11), 640. https://doi.org/10.3390/fermentation8110640

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Improved Sugar Recovery of Alkaline Pre-Treated Pineapple Leaf Fibres via Enzymatic Hydrolysis and Its Enzymatic Kinetics

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI