Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites

Setiawan, Andi; Widyastuti, Widyastuti; Irawan, Arik; Wijaya, Oklis Syahrin; Laila, Aspita; Setiawan, Wawan Abdullah; Juliasih, Ni Luh Gede Ratna; Nonaka, Kenichi; Arai, Masayoshi; Hendri, John

doi:10.3390/fermentation7040247

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Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites

Andi Setiawan

Widyastuti Widyastuti

¹,

Arik Irawan

Oklis Syahrin Wijaya

Aspita Laila

¹,

Wawan Abdullah Setiawan

²,

Ni Luh Gede Ratna Juliasih

Kenichi Nonaka

³,

Masayoshi Arai

⁴

and

John Hendri

^1,*

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Lampung University, Bandar Lampung 35145, Indonesia

Department of Biology, Faculty of Mathematics and Natural Science, Lampung University, Bandar Lampung 35145, Indonesia

Ōmura Satoshi Memorial Institute, Kitasato University, 5-9-1 Shirokane Minato-ku, Tokyo 108-8641, Japan

⁴

Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita 565-0871, Japan

Author to whom correspondence should be addressed.

Academic Editor: Thaddeus Ezeji

Fermentation 2021, 7(4), 247; https://doi.org/10.3390/fermentation7040247

Received: 8 September 2021 / Revised: 25 September 2021 / Accepted: 14 October 2021 / Published: 29 October 2021

(This article belongs to the Special Issue Fermentation and Bioactive Metabolites 3.0)

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Abstract

Marine actinomycetes are prolific microorganisms; however, knowledge of their diversity, distribution, and secondary metabolites is limited. Marine actinomycetes represent an untapped source of novel bioactive compounds. In this study, we investigated shrimp shell as substrates for model production bioactive metabolites from actinomycetes under solid state fermentation (SSF) conditions. A total of fifteen actinomycetes were isolated from six sponges and one tunicate. The isolated actinomycetes were grown on solid shrimp shells. Cultures of actinomycetes were extracted with ethyl acetate (EtOAc) and extracts were bioassayed for activity against Staphylococcus aureus. One isolate 18A13O1 from the sponge, Rhabdastrella globostellata, exhibited antibacterial activity on primary screening compared to the other samples and was chosen for further study. Visualization using SEM showed aerial and substrate mycelia. Through phylogenetic analysis, it was confirmed that isolate 18A13O1 is a Pseudonocardia carboxydivorans. Purification of an EtOAc extract yielded A13B2, which showed a minimum inhibition concentration against S. aureus at 15.6 μg/mL. It can be concluded that this basic information is very important for further studies related to the development of the production of bioactive secondary metabolites through the solid state fermentation process. View Full-Text

Keywords: actinomycetes; antibacteria; Staphylococcus aureus; solid state cultivation

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MDPI and ACS Style

Setiawan, A.; Widyastuti, W.; Irawan, A.; Wijaya, O.S.; Laila, A.; Setiawan, W.A.; Juliasih, N.L.G.R.; Nonaka, K.; Arai, M.; Hendri, J. Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites. Fermentation 2021, 7, 247. https://doi.org/10.3390/fermentation7040247

AMA Style

Setiawan A, Widyastuti W, Irawan A, Wijaya OS, Laila A, Setiawan WA, Juliasih NLGR, Nonaka K, Arai M, Hendri J. Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites. Fermentation. 2021; 7(4):247. https://doi.org/10.3390/fermentation7040247

Chicago/Turabian Style

Setiawan, Andi, Widyastuti Widyastuti, Arik Irawan, Oklis S. Wijaya, Aspita Laila, Wawan A. Setiawan, Ni L.G.R. Juliasih, Kenichi Nonaka, Masayoshi Arai, and John Hendri. 2021. "Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites" Fermentation 7, no. 4: 247. https://doi.org/10.3390/fermentation7040247

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Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites

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