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Open AccessArticle

Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust

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Instituto de Investigación y Desarrollo de Procesos Químicos, Facultad de Ingeniería, Universidad Mayor de San Andrés, P.O. Box 12958 La Paz, Bolivia
2
Division of Biotechnology, Faculty of Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden
3
National Nanotechnology Laboratory, National Center for High Technology, San José 10109, Costa Rica
4
Planta Piloto de Bioprocesos, Facultad de Ciencias y Tecnología, Universidad Mayor de San Simón, Cochabamba, Bolivia
5
Department of Chemistry, Umeå University, P.O. Box 901 87 Umeå, Sweden
*
Authors to whom correspondence should be addressed.
First authorship shared by D.C. and L.R.-S.
Academic Editor: Diomi Mamma
Fermentation 2021, 7(1), 33; https://doi.org/10.3390/fermentation7010033
Received: 19 January 2021 / Revised: 22 February 2021 / Accepted: 24 February 2021 / Published: 28 February 2021
(This article belongs to the Special Issue Food Wastes: Feedstock for Value-Added Products 2.0)
The halotolerant bacterial strain BU-4, isolated from a hypersaline environment, was identified as an exopolysaccharide (EPS) producer. Pretreatment liquids of steam-exploded quinoa stalks and enzymatic hydrolysates of Curupaú sawdust were evaluated as carbon sources for EPS production with the BU-4 strain, and the produced EPS was characterized using FTIR, TGA, and SEM. Cultivation was performed at 30 °C for 48 h, and the cells were separated from the culture broth by centrifugation. EPS was isolated from the cell pellets by ethanol precipitation, and purified by trichloroacetic acid treatment, followed by centrifugation, dialysis, and freeze-drying. EPS production from quinoa stalks- and Curupaú sawdust-based substrates was 2.73 and 0.89 g L−1, respectively, while 2.34 g L−1 was produced when cultivation was performed on glucose. FTIR analysis of the EPS revealed signals typical for polysaccharides, as well as ester carbonyl groups and sulfate groups. High thermal stability, water retention capacity and gel-forming ability were inferred from SEM and TGA. The capability of the halotolerant isolate for producing EPS from pretreatment liquids and hydrolysates was demonstrated, and characterization of the EPS revealed their broad application potential. The study shows a way for producing value-added products from waste materials using a bacterium from a unique Bolivian ecosystem. View Full-Text
Keywords: exopolysaccharides; bacterial cultivation; halotolerant bacterial isolate; Salar de Uyuni; quinoa stalks; Curupaú sawdust; lignocellulose bioconversion exopolysaccharides; bacterial cultivation; halotolerant bacterial isolate; Salar de Uyuni; quinoa stalks; Curupaú sawdust; lignocellulose bioconversion
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MDPI and ACS Style

Chambi, D.; Romero-Soto, L.; Villca, R.; Orozco-Gutiérrez, F.; Vega-Baudrit, J.; Quillaguamán, J.; Hatti-Kaul, R.; Martín, C.; Carrasco, C. Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust. Fermentation 2021, 7, 33. https://doi.org/10.3390/fermentation7010033

AMA Style

Chambi D, Romero-Soto L, Villca R, Orozco-Gutiérrez F, Vega-Baudrit J, Quillaguamán J, Hatti-Kaul R, Martín C, Carrasco C. Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust. Fermentation. 2021; 7(1):33. https://doi.org/10.3390/fermentation7010033

Chicago/Turabian Style

Chambi, Diego; Romero-Soto, Luis; Villca, Roxana; Orozco-Gutiérrez, Felipe; Vega-Baudrit, José; Quillaguamán, Jorge; Hatti-Kaul, Rajni; Martín, Carlos; Carrasco, Cristhian. 2021. "Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust" Fermentation 7, no. 1: 33. https://doi.org/10.3390/fermentation7010033

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