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Open AccessArticle

Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid

Bio-Organic Chemistry Unit, Institute of Biomolecular Chemistry CNR, Via Campi Flegrei 34, Pozzuoli, 80078 Naples, Italy
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These authors have equally contributed to this work.
Fermentation 2019, 5(2), 34; https://doi.org/10.3390/fermentation5020034
Received: 28 February 2019 / Revised: 3 April 2019 / Accepted: 6 April 2019 / Published: 11 April 2019
(This article belongs to the Special Issue Lactic Acid Fermentation and the Colours of Biotechnology)
The industrial production of lactic acid (LA) is mainly based on bacterial fermentation. This process can result in enantiopure or racemic mixture according to the producing organism. Between the enantiomers, L-lactic acid shows superior market value. Recently, we reported a novel anaplerotic pathway called capnophilic lactic fermentation (CLF) that produces a high concentration of LA by fermentation of sugar in the anaerobic thermophilic bacterium Thermotoga neapolitana. The aim of this work was the identification of the enantiomeric characterization of the LA produced by T. neapolitana and identification of the lactate dehydrogenase in T. neapolitana (TnLDH) and related bacteria of the order Thermotogales. Chemical derivatization and GC/MS analysis were applied to define the stereochemistry of LA from T. neapolitana. A bioinformatics study on TnLDH was carried out for the characterization of the enzyme. Chemical analysis showed a 95.2% enantiomeric excess of L-LA produced by T. neapolitana. A phylogenetic approach clearly clustered the TnLDH together with the L-LDH from lactic acid bacteria. We report for the first time that T. neapolitana is able to produce almost enantiopure L-lactic acid. The result was confirmed by bioinformatics analysis on TnLDH, which is a member of the L-LDH sub-family. View Full-Text
Keywords: optical purity; lactate dehydrogenase; stereochemistry; fermentation; thermophilic bacteria optical purity; lactate dehydrogenase; stereochemistry; fermentation; thermophilic bacteria
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Nuzzo, G.; Landi, S.; Esercizio, N.; Manzo, E.; Fontana, A.; d’Ippolito, G. Capnophilic Lactic Fermentation from Thermotoga neapolitana: A Resourceful Pathway to Obtain Almost Enantiopure L-lactic Acid. Fermentation 2019, 5, 34.

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