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Open AccessArticle

Enhanced Production and Quantitative Evaluation of Nigericin from the Algerian Soil-Living Streptomyces youssoufiensis SF10 Strain

1
Laboratory of Bioorganic Chemistry, Department of Physics, University of Trento, via Sommarive 14, I-38123 Povo-Trento, Italy
2
Microbiological Laboratory Engineering and Application, Mentouri Brothers University, Constantine 25000, Algeria
3
Department of Cellular and Molecular Biology, University Abbes Laghrour Khenchela, Khenchela 40000, Algeria
4
Laboratory of Translational Genomics, Department CIBIO, University of Trento, Via Sommarive 9, I-38123 Povo-Trento, Italy
*
Author to whom correspondence should be addressed.
Fermentation 2019, 5(1), 13; https://doi.org/10.3390/fermentation5010013
Received: 18 December 2018 / Revised: 17 January 2019 / Accepted: 22 January 2019 / Published: 26 January 2019
(This article belongs to the Special Issue Fermentation and Bioactive Metabolites)
Nigericin, one of the main ionophoric polyethers produced by various Streptomyces strains, presents relevant biological activities including antibacterial and recently studied antitumor properties. This work describes the influence of different culture conditions on the production of this metabolite by Streptomyces sp. SF10, isolated from a semi-arid soil sample collected at Chélia Mountain, in Khenchela (Northeastern Algeria) and identified as Streptomyces youssoufiensis. The extracts from the strain, cultured in a solid state or submerged fermentation conditions, using several carbon sources at different pH values, in the presence or absence of iron (II) sulfate and in co-culture with other Streptomyces species, were analyzed using a high-performance liquid chromatography (HPLC) system equipped with an evaporative light scattering detector (ELSD). The best culture conditions provided a concentration of nigericin of 0.490 ± 0.001 mg/mL in the extract. The HPLC-ELSD method, optimized here for the quantitative detection of nigericin, can find wider applications in the analysis of several other metabolites characterized by a similar polycyclic polyether structure or, more generally, by the lack of significant chromophores in their molecular structure. View Full-Text
Keywords: polyether ionophores; nigericin; HPLC analysis; Actinobacteria; Streptomyces; fermentation; culture media polyether ionophores; nigericin; HPLC analysis; Actinobacteria; Streptomyces; fermentation; culture media
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MDPI and ACS Style

Leulmi, N.; Sighel, D.; Defant, A.; Khenaka, K.; Boulahrouf, A.; Mancini, I. Enhanced Production and Quantitative Evaluation of Nigericin from the Algerian Soil-Living Streptomyces youssoufiensis SF10 Strain. Fermentation 2019, 5, 13.

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