The carP lncRNA Is a carS-Related Regulatory Element with Broad Effects on the Fusarium fujikuroi Transcriptome

Pardo-Medina, Javier; Gutiérrez, Gabriel; Limón, M. Carmen; Avalos, Javier

doi:10.3390/ncrna7030046

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

The carP lncRNA Is a carS-Related Regulatory Element with Broad Effects on the Fusarium fujikuroi Transcriptome

and

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Seville, Spain

Author to whom correspondence should be addressed.

Academic Editors: Simon Conn and Brian D. Gregory

Non-Coding RNA 2021, 7(3), 46; https://doi.org/10.3390/ncrna7030046

Received: 28 April 2021 / Revised: 23 July 2021 / Accepted: 29 July 2021 / Published: 2 August 2021

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

Carotenoid biosynthesis in the fungus Fusarium fujikuroi is regulated by environmental factors, with light being the main stimulating signal. The CarS RING-finger protein plays an important role in the downregulation of structural genes of the carotenoid pathway. A recent transcriptomic analysis on the effect of carS mutation identified a gene for a long non-coding RNA (lncRNA) upstream of carS, called carP, the deletion of which results in increased carS mRNA levels and lack of carotenoid production. We have investigated the function of carP by studying the transcriptomic effect of its deletion and the phenotypes resulting from the reintroduction of carP to a deletion strain. The RNA-seq data showed that the loss of carP affected the mRNA levels of hundreds of genes, especially after illumination. Many of these changes appeared to be cascade effects as a result of changes in carS expression, as suggested by the comparison with differentially expressed genes in a carS mutant. Carotenoid production only recovered when carP was integrated upstream of carS, but not at other genomic locations, indicating a cis-acting mechanism on carS. However, some genes hardly affected by CarS were strongly upregulated in the carP mutant, indicating that carP may have other regulatory functions as an independent regulatory element. View Full-Text

Keywords: RNA-seq; carP lncRNA; cis-acting mechanism; carotenoid biosynthesis; light regulation

▼ Show Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary File 1:
ZIP-Document (ZIP, 7295 KiB)

Share and Cite

MDPI and ACS Style

Pardo-Medina, J.; Gutiérrez, G.; Limón, M.C.; Avalos, J. The carP lncRNA Is a carS-Related Regulatory Element with Broad Effects on the Fusarium fujikuroi Transcriptome. Non-Coding RNA 2021, 7, 46. https://doi.org/10.3390/ncrna7030046

AMA Style

Pardo-Medina J, Gutiérrez G, Limón MC, Avalos J. The carP lncRNA Is a carS-Related Regulatory Element with Broad Effects on the Fusarium fujikuroi Transcriptome. Non-Coding RNA. 2021; 7(3):46. https://doi.org/10.3390/ncrna7030046

Chicago/Turabian Style

Pardo-Medina, Javier, Gabriel Gutiérrez, M. Carmen Limón, and Javier Avalos. 2021. "The carP lncRNA Is a carS-Related Regulatory Element with Broad Effects on the Fusarium fujikuroi Transcriptome" Non-Coding RNA 7, no. 3: 46. https://doi.org/10.3390/ncrna7030046

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

The carP lncRNA Is a carS-Related Regulatory Element with Broad Effects on the Fusarium fujikuroi Transcriptome

Abstract

Supplementary Material

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI