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Human Brain Shows Recurrent Non-Canonical MicroRNA Editing Events Enriched for Seed Sequence with Possible Functional Consequence

CSIR-Institute of Genomics & Integrative Biology, Delhi 110025, India
Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh 201002, India
Translational Medicine Unit, Biomedical Research Centre, University of Salford, Manchester M5 4WT, UK
Authors to whom correspondence should be addressed.
Non-Coding RNA 2020, 6(2), 21;
Received: 28 April 2020 / Revised: 25 May 2020 / Accepted: 27 May 2020 / Published: 2 June 2020
(This article belongs to the Special Issue Non-Coding RNAs: Variations and Disease)
RNA editing is a post-transcriptional modification, which can provide tissue-specific functions not encoded in DNA. Adenosine-to-inosine is the predominant editing event and, along with cytosine-to-uracil changes, constitutes canonical editing. The rest is non-canonical editing. In this study, we have analysed non-canonical editing of microRNAs in the human brain. We have performed massively parallel small RNA sequencing of frontal cortex (FC) and corpus callosum (CC) pairs from nine normal individuals (post-mortem). We found 113 and 90 unique non-canonical editing events in FC and CC samples, respectively. More than 70% of events were in the miRNA seed sequence—implicating an altered set of target mRNAs and possibly resulting in a functional consequence. Up to 15% of these events were recurring and found in at least three samples, also supporting the biological relevance of such variations. Two specific sequence variations, C-to-A and G-to-U, accounted for over 80% of non-canonical miRNA editing events—and revealed preferred sequence motifs. Our study is one of the first reporting non-canonical editing in miRNAs in the human brain. Our results implicate miRNA non-canonical editing as one of the contributing factors towards transcriptomic diversity in the human brain. View Full-Text
Keywords: microRNA; RNA editing; human brain microRNA; RNA editing; human brain
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Paul, D.; Ansari, A.H.; Lal, M.; Mukhopadhyay, A. Human Brain Shows Recurrent Non-Canonical MicroRNA Editing Events Enriched for Seed Sequence with Possible Functional Consequence. Non-Coding RNA 2020, 6, 21.

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