Next Article in Journal
Hypoxia-Induced MicroRNA-210 Targets Neurodegenerative Pathways
Next Article in Special Issue
Long Non-Coding RNAs in Obesity-Induced Cancer
Previous Article in Journal / Special Issue
RNA Surveillance by the Nuclear RNA Exosome: Mechanisms and Significance
Open AccessFeature PaperReview

Strengths and Weaknesses of the Current Strategies to Map and Characterize R-Loops

UMR5239 CNRS, Université de Lyon, ENS-Lyon, 46 allée d’Italie, 69007 Lyon, France
Non-Coding RNA 2018, 4(2), 9;
Received: 1 March 2018 / Revised: 22 March 2018 / Accepted: 23 March 2018 / Published: 27 March 2018
(This article belongs to the Special Issue Genomic Instability and Non-Coding RNA)
R-loops are evolutionarily conserved three-stranded structures that result from the formation of stable DNA:RNA hybrids in the genome. R-loops have attracted increasing interest in recent years as potent regulators of gene expression and genome stability. In particular, their strong association with severe replication stress makes them potential oncogenic structures. Despite their importance, the rules that govern their formation and their dynamics are still controversial and an in-depth description of their direct impact on chromatin organization and DNA transactions is still lacking. To better understand the diversity of R-loop functions, reliable, accurate, and quantitative mapping techniques, as well as functional assays are required. Here, I review the different approaches that are currently used to do so and to highlight their individual strengths and weaknesses. In particular, I review the advantages and disadvantages of using the S9.6 antibody to map R-loops in vivo in an attempt to propose guidelines for best practices. View Full-Text
Keywords: R-loops; S9.6; RNase H1; DRIP; R-ChIP R-loops; S9.6; RNase H1; DRIP; R-ChIP
Show Figures

Graphical abstract

MDPI and ACS Style

Vanoosthuyse, V. Strengths and Weaknesses of the Current Strategies to Map and Characterize R-Loops. Non-Coding RNA 2018, 4, 9.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop