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Article

A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples

by 1,2,3,*,†, 1,2,3,†, 1,2,3, 2,3 and 1,4,*
1
Leibniz Institute on Aging—Fritz Lipmann Institute, 07745 Jena, Germany
2
Faculty of Medicine, Friedrich-Schiller-University Jena, 07743 Jena, Germany
3
Institute of Molecular Cell Biology, University Hospital Jena, Friedrich-Schiller-University, 07745 Jena, Germany
4
International Center for Aging and Cancer, Hainan Medical University, Haikou 571199, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Hiroyuki Takeno
Received: 28 November 2021 / Revised: 20 December 2021 / Accepted: 1 January 2022 / Published: 7 January 2022
(This article belongs to the Special Issue Advance in Composite Gels)
Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA) assay tends to underestimate protein concentrations of tissue samples. We present a Ponceau S staining-based dot-blot assay as an alternative for protein quantification. This method is simple, rapid, more reliable than the BCA assay, compatible with biological samples lysed in RIPA or 2x SDS gel-loading buffer, and also inexpensive. View Full-Text
Keywords: protein quantification; gel electrophoresis; Western blot; dot blot protein quantification; gel electrophoresis; Western blot; dot blot
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MDPI and ACS Style

Helbing, D.L.; Böhm, L.; Oraha, N.; Stabenow, L.K.; Cui, Y. A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples. Gels 2022, 8, 43. https://doi.org/10.3390/gels8010043

AMA Style

Helbing DL, Böhm L, Oraha N, Stabenow LK, Cui Y. A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples. Gels. 2022; 8(1):43. https://doi.org/10.3390/gels8010043

Chicago/Turabian Style

Helbing, Dario L., Leopold Böhm, Nova Oraha, Leonie K. Stabenow, and Yan Cui. 2022. "A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples" Gels 8, no. 1: 43. https://doi.org/10.3390/gels8010043

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