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Article

The Siderophore Transporters Sit1 and Sit2 Are Essential for Utilization of Ferrichrome-, Ferrioxamine- and Coprogen-Type Siderophores in Aspergillus fumigatus

by
Mario Aguiar
,
Thomas Orasch
,
Matthias Misslinger
,
Anna-Maria Dietl
,
Fabio Gsaller
and
Hubertus Haas
*
Institute of Molecular Biology/Biocenter, Medical University of Innsbruck, A-6020 Innsbruck, Austria
*
Author to whom correspondence should be addressed.
J. Fungi 2021, 7(9), 768; https://doi.org/10.3390/jof7090768
Submission received: 26 August 2021 / Revised: 13 September 2021 / Accepted: 14 September 2021 / Published: 16 September 2021
(This article belongs to the Special Issue Molecular Processes of Fungi)
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Abstract

Siderophore-mediated acquisition of iron has been shown to be indispensable for the virulence of several fungal pathogens, the siderophore transporter Sit1 was found to mediate uptake of the novel antifungal drug VL-2397, and siderophores were shown to be useful as biomarkers as well as for imaging of fungal infections. However, siderophore uptake in filamentous fungi is poorly characterized. The opportunistic human pathogen Aspergillus fumigatus possesses five putative siderophore transporters. Here, we demonstrate that the siderophore transporters Sit1 and Sit2 have overlapping, as well as unique, substrate specificities. With respect to ferrichrome-type siderophores, the utilization of ferrirhodin and ferrirubin depended exclusively on Sit2, use of ferrichrome A depended mainly on Sit1, and utilization of ferrichrome, ferricrocin, and ferrichrysin was mediated by both transporters. Moreover, both Sit1 and Sit2 mediated use of the coprogen-type siderophores coprogen and coprogen B, while only Sit1 transported the bacterial ferrioxamine-type xenosiderophores ferrioxamines B, G, and E. Neither Sit1 nor Sit2 were important for the utilization of the endogenous siderophores fusarinine C and triacetylfusarinine C. Furthermore, A. fumigatus was found to lack utilization of the xenosiderophores schizokinen, basidiochrome, rhizoferrin, ornibactin, rhodotorulic acid, and enterobactin. Taken together, this study characterized siderophore use by A. fumigatus and substrate characteristics of Sit1 and Sit2.
Keywords: fungi; molds; Aspergillus fumigatus; iron; siderophore; xenosiderophores; transporter fungi; molds; Aspergillus fumigatus; iron; siderophore; xenosiderophores; transporter

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MDPI and ACS Style

Aguiar, M.; Orasch, T.; Misslinger, M.; Dietl, A.-M.; Gsaller, F.; Haas, H. The Siderophore Transporters Sit1 and Sit2 Are Essential for Utilization of Ferrichrome-, Ferrioxamine- and Coprogen-Type Siderophores in Aspergillus fumigatus. J. Fungi 2021, 7, 768. https://doi.org/10.3390/jof7090768

AMA Style

Aguiar M, Orasch T, Misslinger M, Dietl A-M, Gsaller F, Haas H. The Siderophore Transporters Sit1 and Sit2 Are Essential for Utilization of Ferrichrome-, Ferrioxamine- and Coprogen-Type Siderophores in Aspergillus fumigatus. Journal of Fungi. 2021; 7(9):768. https://doi.org/10.3390/jof7090768

Chicago/Turabian Style

Aguiar, Mario, Thomas Orasch, Matthias Misslinger, Anna-Maria Dietl, Fabio Gsaller, and Hubertus Haas. 2021. "The Siderophore Transporters Sit1 and Sit2 Are Essential for Utilization of Ferrichrome-, Ferrioxamine- and Coprogen-Type Siderophores in Aspergillus fumigatus" Journal of Fungi 7, no. 9: 768. https://doi.org/10.3390/jof7090768

APA Style

Aguiar, M., Orasch, T., Misslinger, M., Dietl, A.-M., Gsaller, F., & Haas, H. (2021). The Siderophore Transporters Sit1 and Sit2 Are Essential for Utilization of Ferrichrome-, Ferrioxamine- and Coprogen-Type Siderophores in Aspergillus fumigatus. Journal of Fungi, 7(9), 768. https://doi.org/10.3390/jof7090768

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