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Article

Transcriptional Analysis of Coccidioides immitis Mycelia and Spherules by RNA Sequencing

1
Department of Medicine, Division of Infectious Disease, U.C. San Diego School of Medicine, La Jolla, CA 92093, USA
2
J. Craig Venter Institute, La Jolla, CA 92037, USA
3
Department of Microbiology and Plant Pathology, Institute for Integrative Genome Biology, University of California-Riverside, Riverside, CA 92521, USA
4
Infectious Diseases Section, VA Healthcare San Diego, San Diego, CA 92161, USA
5
Department of Pathology, U.C. San Diego School of Medicine, La Jolla, CA 92093, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Aitor D. Rementeria-Ruiz
J. Fungi 2021, 7(5), 366; https://doi.org/10.3390/jof7050366
Received: 27 March 2021 / Revised: 13 April 2021 / Accepted: 22 April 2021 / Published: 7 May 2021
(This article belongs to the Special Issue Coccidioides and Coccidioidomycosis 2020)
Coccidioides immitis and C. posadasii are dimorphic fungi that transform from mycelia with internal arthroconidia in the soil to a tissue form known as a spherule in mammals. This process can be recapitulated in vitro by increasing the temperature, CO2 and changing other culture conditions. In this study, we have analyzed changes in gene expression in mycelia and young and mature spherules. Genes that were highly upregulated in young spherules include a spherule surface protein and iron and copper membrane transporters. Genes that are unique to Coccidioides spp. are also overrepresented in this group, suggesting that they may be important for spherule differentiation. Enriched GO terms in young spherule upregulated genes include oxidation-reduction, response to stress and membrane proteins. Downregulated genes are enriched for transcription factors, especially helix–loop–helix and C2H2 type zinc finger domain-containing proteins, which is consistent with the dramatic change in transcriptional profile. Almost all genes that are upregulated in young spherules remain upregulated in mature spherules, but a small number of genes are differentially expressed in those two stages of spherule development. Mature spherules express more Hsp31 and amylase and less tyrosinase than young spherules. Some expression of transposons was detected and most of the differentially expressed transposons were upregulated in spherules. View Full-Text
Keywords: Coccidioides immitis; coccidioidomycosis; fungus; dimorphic fungus; mycelium; spherule; differentiation; RNA-Seq; differential gene expression Coccidioides immitis; coccidioidomycosis; fungus; dimorphic fungus; mycelium; spherule; differentiation; RNA-Seq; differential gene expression
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MDPI and ACS Style

Carlin, A.F.; Beyhan, S.; Peña, J.F.; Stajich, J.E.; Viriyakosol, S.; Fierer, J.; Kirkland, T.N. Transcriptional Analysis of Coccidioides immitis Mycelia and Spherules by RNA Sequencing. J. Fungi 2021, 7, 366. https://doi.org/10.3390/jof7050366

AMA Style

Carlin AF, Beyhan S, Peña JF, Stajich JE, Viriyakosol S, Fierer J, Kirkland TN. Transcriptional Analysis of Coccidioides immitis Mycelia and Spherules by RNA Sequencing. Journal of Fungi. 2021; 7(5):366. https://doi.org/10.3390/jof7050366

Chicago/Turabian Style

Carlin, Aaron F., Sinem Beyhan, Jesús F. Peña, Jason E. Stajich, Suganya Viriyakosol, Joshua Fierer, and Theo N. Kirkland 2021. "Transcriptional Analysis of Coccidioides immitis Mycelia and Spherules by RNA Sequencing" Journal of Fungi 7, no. 5: 366. https://doi.org/10.3390/jof7050366

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