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Article

Identification of the Ricin-B-Lectin LdRBLk in the Colorado Potato Beetle and an Analysis of Its Expression in Response to Fungal Infections

Institute of Systematics and Ecology of Animals of Siberian Branch of the Russian Academy of Science, Frunze Str. 11, 630091 Novosibirsk, Russia
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Academic Editor: Joseph M. Bliss
J. Fungi 2021, 7(5), 364; https://doi.org/10.3390/jof7050364
Received: 1 April 2021 / Revised: 26 April 2021 / Accepted: 4 May 2021 / Published: 6 May 2021
(This article belongs to the Special Issue Host Defense against Fungi)
Ricin-B-lectins (RBLs) have been identified in many groups of organisms, including coleopterans insects, particularly the Colorado potato beetle Leptinotarsa decemlineata (LdRBLs). We hypothesized that one of these LdRBLs (LdRBLk) may be involved in the immune response to fungal infections. We performed a theoretical analysis of the structure of this protein. Additionally, the expression levels of the LdRBlk gene were measured in L. decemlineata in response to infections with the fungi Metarhizium robertsii and Beauveria bassiana. The expression levels of LdRBlk in the L. decemlineata cuticle and fat body were increased in response to both infections. The induction of LdRBlk expression was dependent on the susceptibility of larvae to the fungi. Upregulation of the LdRBlk gene was also observed in response to other stresses, particularly thermal burns. Elevation of LdRBlk expression was frequently observed to be correlated with the expression of the antimicrobial peptide attacin but was not correlated with hsp90 regulation. Commercially available β-lectin of ricin from Ricinuscommunis was observed to inhibit the germination of conidia of the fungi. We suggest that LdRBLk is involved in antifungal immune responses in the Colorado potato beetle, either exerting fungicidal properties directly or acting as a modulator of the immune response. View Full-Text
Keywords: antifungal peptide; attacin; C-type lectins; heat shock protein; domain pfam00652; RCA antifungal peptide; attacin; C-type lectins; heat shock protein; domain pfam00652; RCA
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MDPI and ACS Style

Rotskaya, U.N.; Kryukov, V.Y.; Kosman, E.; Tyurin, M.; Glupov, V.V. Identification of the Ricin-B-Lectin LdRBLk in the Colorado Potato Beetle and an Analysis of Its Expression in Response to Fungal Infections. J. Fungi 2021, 7, 364. https://doi.org/10.3390/jof7050364

AMA Style

Rotskaya UN, Kryukov VY, Kosman E, Tyurin M, Glupov VV. Identification of the Ricin-B-Lectin LdRBLk in the Colorado Potato Beetle and an Analysis of Its Expression in Response to Fungal Infections. Journal of Fungi. 2021; 7(5):364. https://doi.org/10.3390/jof7050364

Chicago/Turabian Style

Rotskaya, Ulyana N., Vadim Y. Kryukov, Elena Kosman, Maksim Tyurin, and Viktor V. Glupov. 2021. "Identification of the Ricin-B-Lectin LdRBLk in the Colorado Potato Beetle and an Analysis of Its Expression in Response to Fungal Infections" Journal of Fungi 7, no. 5: 364. https://doi.org/10.3390/jof7050364

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