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Article

Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization

Centro de Investigaciones Biológicas Margarita Salas (CIB), Consejo Superior de Investigaciones Científicas (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain
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Academic Editor: Baojun Xu
J. Fungi 2021, 7(5), 363; https://doi.org/10.3390/jof7050363
Received: 14 April 2021 / Revised: 28 April 2021 / Accepted: 3 May 2021 / Published: 6 May 2021
(This article belongs to the Special Issue Fungal Biotechnology and Application)
As β-glucosidases represent the major bottleneck for the industrial degradation of plant biomass, great efforts are being devoted to discover both novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme β-glucosidase-enriched cocktails. Approximately 11 U/mL β-glucosidase was detected in these cultures, constituting the major cellulolytic activity. Proteomic analysis showed BGL-3 as the most abundant protein and the main β-glucosidase. This crude enzyme was successfully used to supplement a basal commercial cellulolytic cocktail (Celluclast 1.5 L) for saccharification of pretreated wheat straw, corroborating that even hardly exploitable industrial wastes, such as glycerol, can be used as secondary raw materials to produce valuable enzymatic preparations in a framework of the circular economy. View Full-Text
Keywords: biodiesel by-products; cellulases; fungi; hydrolases; wastes biodiesel by-products; cellulases; fungi; hydrolases; wastes
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MDPI and ACS Style

Méndez-Líter, J.A.; de Eugenio, L.I.; Hakalin, N.L.S.; Prieto, A.; Martínez, M.J. Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization. J. Fungi 2021, 7, 363. https://doi.org/10.3390/jof7050363

AMA Style

Méndez-Líter JA, de Eugenio LI, Hakalin NLS, Prieto A, Martínez MJ. Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization. Journal of Fungi. 2021; 7(5):363. https://doi.org/10.3390/jof7050363

Chicago/Turabian Style

Méndez-Líter, Juan A., Laura I. de Eugenio, Neumara L. S. Hakalin, Alicia Prieto, and María Jesús Martínez. 2021. "Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization" Journal of Fungi 7, no. 5: 363. https://doi.org/10.3390/jof7050363

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