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Article

Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices

1
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Universidad Complutense de Madrid, 28040 Madrid, Spain
2
TECNALIA, Basque Research and Technology Alliance (BRTA), E20009 Donostia-San Sebastian, Spain
3
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain
4
CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, 28040 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Joseph M. Bliss
J. Fungi 2021, 7(5), 392; https://doi.org/10.3390/jof7050392
Received: 12 April 2021 / Revised: 12 May 2021 / Accepted: 15 May 2021 / Published: 17 May 2021
(This article belongs to the Special Issue Host Defense against Fungi)
Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario. View Full-Text
Keywords: Candida albicans; macrophage; extracellular matrix; decellularization; immunocompetence; phagocytosis Candida albicans; macrophage; extracellular matrix; decellularization; immunocompetence; phagocytosis
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MDPI and ACS Style

Cicuéndez, M.; Casarrubios, L.; Feito, M.J.; Madarieta, I.; Garcia-Urkia, N.; Murua, O.; Olalde, B.; Briz, N.; Diez-Orejas, R.; Portolés, M.T. Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices. J. Fungi 2021, 7, 392. https://doi.org/10.3390/jof7050392

AMA Style

Cicuéndez M, Casarrubios L, Feito MJ, Madarieta I, Garcia-Urkia N, Murua O, Olalde B, Briz N, Diez-Orejas R, Portolés MT. Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices. Journal of Fungi. 2021; 7(5):392. https://doi.org/10.3390/jof7050392

Chicago/Turabian Style

Cicuéndez, Mónica, Laura Casarrubios, María J. Feito, Iratxe Madarieta, Nerea Garcia-Urkia, Olatz Murua, Beatriz Olalde, Nerea Briz, Rosalía Diez-Orejas, and María T. Portolés 2021. "Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices" Journal of Fungi 7, no. 5: 392. https://doi.org/10.3390/jof7050392

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