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Article

Archaeal tRNA-Splicing Endonuclease as an Effector for RNA Recombination and Novel Trans-Splicing Pathways in Eukaryotes

by
Giuseppe D. Tocchini-Valentini
1,2,* and
Glauco P. Tocchini-Valentini
1,2
1
Istituto di Biochimica e Biologia Cellulare, Campus Internazionale “A. Buzzati-Traverso”, Dipartimento Scienze Biomediche, Consiglio Nazionale delle Ricerche, via Ramarini 32, 00015 Monterotondo, Rome, Italy
2
Dipartimento Scienze Biomediche, European Mouse Mutant Archive (EMMA), INFRAFRONTIER-IMPC, Monterotondo Mouse Clinic, Campus Internazionale “A. Buzzati-Traverso”, Consiglio Nazionale delle Ricerche, via Ramarini 32, 00015 Monterotondo, Rome, Italy
*
Author to whom correspondence should be addressed.
J. Fungi 2021, 7(12), 1069; https://doi.org/10.3390/jof7121069
Submission received: 20 November 2021 / Revised: 9 December 2021 / Accepted: 9 December 2021 / Published: 12 December 2021
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Abstract

We have characterized a homodimeric tRNA endonuclease from the euryarchaeota Ferroplasma acidarmanus (FERAC), a facultative anaerobe which can grow at temperatures ranging from 35 to 42 °C. This enzyme, contrary to the eukaryal tRNA endonucleases and the homotetrameric Methanocaldococcus jannaschii (METJA) homologs, is able to cleave minimal BHB (bulge–helix–bulge) substrates at 30 °C. The expression of this enzyme in Schizosaccharomyces pombe (SCHPO) enables the use of its properties as effectors by inserting BHB motif introns into hairpin loops normally seen in mRNA transcripts. In addition, the FERAC endonuclease can create proteins with new functionalities through the recombination of protein domains.
Keywords: tRNA-splicing endonuclease; RNA processing; RNA recombination tRNA-splicing endonuclease; RNA processing; RNA recombination

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MDPI and ACS Style

Tocchini-Valentini, G.D.; Tocchini-Valentini, G.P. Archaeal tRNA-Splicing Endonuclease as an Effector for RNA Recombination and Novel Trans-Splicing Pathways in Eukaryotes. J. Fungi 2021, 7, 1069. https://doi.org/10.3390/jof7121069

AMA Style

Tocchini-Valentini GD, Tocchini-Valentini GP. Archaeal tRNA-Splicing Endonuclease as an Effector for RNA Recombination and Novel Trans-Splicing Pathways in Eukaryotes. Journal of Fungi. 2021; 7(12):1069. https://doi.org/10.3390/jof7121069

Chicago/Turabian Style

Tocchini-Valentini, Giuseppe D., and Glauco P. Tocchini-Valentini. 2021. "Archaeal tRNA-Splicing Endonuclease as an Effector for RNA Recombination and Novel Trans-Splicing Pathways in Eukaryotes" Journal of Fungi 7, no. 12: 1069. https://doi.org/10.3390/jof7121069

APA Style

Tocchini-Valentini, G. D., & Tocchini-Valentini, G. P. (2021). Archaeal tRNA-Splicing Endonuclease as an Effector for RNA Recombination and Novel Trans-Splicing Pathways in Eukaryotes. Journal of Fungi, 7(12), 1069. https://doi.org/10.3390/jof7121069

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