Next Article in Journal
A Multidisciplinary Approach to Fungal Infections: One-Year Experiences of a Center of Expertise in Mycology
Next Article in Special Issue
Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation in Colletotrichum gloeosporioides
Previous Article in Journal
Preterm Infants Harbour a Rapidly Changing Mycobiota That Includes Candida Pathobionts
Previous Article in Special Issue
In Vitro or In Vivo Models, the Next Frontier for Unraveling Interactions between Malassezia spp. and Hosts. How Much Do We Know?
Article

Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes

1
Department of R&D Planning & Management, Theragen Bio, Suwon 16229, Korea
2
Department of Biology, Chungbuk National University, Cheongju 28644, Korea
3
Forest Mushroom Research Center, National Forestry Cooperative Federation, Yeoju 12653, Korea
4
Division of Applied Life Science and Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Korea
5
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea
6
Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
J. Fungi 2020, 6(4), 272; https://doi.org/10.3390/jof6040272
Received: 19 October 2020 / Revised: 4 November 2020 / Accepted: 5 November 2020 / Published: 9 November 2020
(This article belongs to the Special Issue Emergent Fungal Models for Genetics and Cell Biology)
Lentinula edodes is a globally important edible mushroom species that is appreciated for its medicinal properties as well as its nutritional value. During commercial cultivation, a mycelial brown film forms on the surface of the sawdust growth medium at the late vegetative stage. Mycelial film formation is a critical developmental process that contributes to the quantity and quality of the mushroom yield. However, little is known regarding the genetic underpinnings of brown film formation on the surface of mycelial tissue. A novel causal gene associated with the formation of the mycelial brown film, named ABL (Abnormal browning related to light), was identified in this study. The comparative genetic analysis by dihybrid crosses between normal and abnormal browning film cultivars demonstrated that a single dominant allele was responsible for the abnormal mycelium browning phenotype. Whole-genome sequencing analysis of hybrid isolates revealed five missense single-nucleotide polymorphisms (SNPs) in the ABL locus of individuals forming abnormal partial brown films. Additional whole-genome resequencing of a further 16 cultivars showed that three of the five missense SNPs were strongly associated with the abnormal browning phenotype. Overexpression of the dominant abl-D allele in a wild-type background conferred the abnormal mycelial browning phenotype upon transformants, with slender hyphae observed as a general defective mycelial growth phenotype. Our methodology will aid the future discovery of candidate genes associated with favorable traits in edible mushrooms. The discovery of a novel gene, ABL, associated with mycelial film formation will facilitate marker-associated breeding in L. edodes. View Full-Text
Keywords: Lentinula edodes; mycelium brown film; abnormal browning related to light Lentinula edodes; mycelium brown film; abnormal browning related to light
Show Figures

Figure 1

MDPI and ACS Style

Hong, C.P.; Moon, S.; Yoo, S.-i.; Noh, J.-H.; Ko, H.-G.; Kim, H.A.; Ro, H.-S.; Cho, H.; Chung, J.-W.; Lee, H.-Y.; Ryu, H. Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes. J. Fungi 2020, 6, 272. https://doi.org/10.3390/jof6040272

AMA Style

Hong CP, Moon S, Yoo S-i, Noh J-H, Ko H-G, Kim HA, Ro H-S, Cho H, Chung J-W, Lee H-Y, Ryu H. Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes. Journal of Fungi. 2020; 6(4):272. https://doi.org/10.3390/jof6040272

Chicago/Turabian Style

Hong, Chang P., Suyun Moon, Seung-il Yoo, Jong-Hyun Noh, Han-Gyu Ko, Hyun A. Kim, Hyeon-Su Ro, Hyunwoo Cho, Jong-Wook Chung, Hwa-Yong Lee, and Hojin Ryu. 2020. "Functional Analysis of a Novel ABL (Abnormal Browning Related to Light) Gene in Mycelial Brown Film Formation of Lentinula edodes" Journal of Fungi 6, no. 4: 272. https://doi.org/10.3390/jof6040272

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop